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Do I need to buy the AutoLys M TubeLifter (Cat. No. A37956) or AutoLys M Tube Pliers (Cat. No. A38261) to use the AutoLys M Tubes (Cat. No. A38738)? Product FAQ

Answer

No, the AutoLys M Tubes can be lifted manually, but the tools significantly reduce hand strain and increase ease of use as well as throughput.

Answer Id:: E16496

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Why do you recommend Nunc Cryobank vials for liquid nitrogen storage and the Matrix ScrewTop storage tubes for storage at higher temperatures? Product FAQ

Answer

We recommend using Nunc Cryobank vials for liquid nitrogen storage as these vials are designed for use at ultra-low temperatures. They come without a lid so that they can be easily picked from the rack at ultra-low temperatures using a tube selection or pen tool.
On the other hand, we recommend Matrix ScrewTop storage tubes for use at higher temperatures (-80 degrees C and above). They work well for both manual and automated picking.

Answer Id:: E17636

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Which plastic consumables should I use on my real-time PCR (qPCR)/thermal cycler (PCR) instrument? Product FAQ

Answer

Please select your real-time PCR (qPCR) or thermal cycler (PCR) instrument model from the drop-down menu in the qPCR and PCR Plastics Selection Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/plastics-selection-guide.html) to see the list of all validated plastic consumables (plates, tube strips, single tubes, caps, seals, adhesive films, and accessories) we offer that are compatible with your instrument.

Answer Id:: E18748

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Single-tube genotyping without oligonucleotide probes. Citations & References

  • Authors: Germer S, Higuchi R
  • Journal: Genome Res
  • PubMed ID: 9927486
Catalog #

Peripheral blood gene expression: it all boils down to the RNA collection tubes. Citations & References

  • Authors: Menke A, Rex-Haffner M, Klengel T, Binder EB, Mehta D
  • Journal: BMC Res Notes
  • PubMed ID: 22214347

Can I order my custom sequences (forward primer, probe, and reverse primer) as a premixed assay in a single tube? Product FAQ

Answer

Yes. For a gene expression assay, you can order here (https://www.thermofisher.com/order/custom-genomic-products/tools/gene-expression/), by selecting the “Enter Custom Primer/Probe Pairs” option. For SNP genotyping assays, you can order here (https://www.thermofisher.com/order/custom-genomic-products/tools/genotyping/) by selecting the “Enter Custom Primer Probe Pairs” option.

Answer Id:: E19553

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Tools for flash-photolysis experiments on voltage-clamped muscle fibre segments. Citations & References

  • Authors: Schuhmeier RP, Tewes S, Szentesi P, Melzer W
  • Journal: Pflugers Arch
  • PubMed ID: 10650992
Catalog # D6814(Discontinued)

Typing of dengue viruses in clinical specimens and mosquitoes by single-tube multiplex reverse transcriptase PCR. Citations & References

  • Authors: Harris E; Roberts TG; Smith L; Selle J; Kramer LD; Valle S; Sandoval E; Balmaseda A
  • Journal: Journal of Clinical Microbiology
Catalog #

Brochure: Element GD Plus Glow Discharge Mass Spectrometer – Defining Quality Standards for the Analysis of Solid Samples Product Literature

Which Thermo Scientific 2D barcoded storage tubes should I use for my application? Product FAQ

Answer

Please view the following information regarding the usage of Thermo Scientific 2D barcoded storage tubes:
Tube Type; Typical Storage Temperature; Application(s); Special Feature(s)
Sepraseal; -20 degrees C; Compound storage; Can easily be removed for sample access
Presplit Sepraseal; -20 degrees C; Compound storage; Up to 200 piercings
Duraseal; -20 degrees; Compound storage; Safe seal for multiple piercings
Matrix ScrewTop; -80 degrees C; Blood components, DNA, Bacteria; With the correct torque, there is very little evaporation at -80 C, disposable pipette tips can be used
Nunc Screwcap; -176 degrees C; Mammalian cells; Minimal height, tubes can be removed with cryo gloves

Answer Id:: E17635

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Human T-cell lines with well-defined T-cell receptor gene rearrangements as controls for the BIOMED-2 multiplex polymerase chain reaction tubes. Citations & References

  • Authors: Sandberg Y; Verhaaf B; van Gastel-Mol EJ; Wolvers-Tettero IL; de Vos J; Macleod RA; Noordzij JG; Dik WA; van Dongen JJ; Langerak AW
  • Journal: Leukemia : Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.k

A diagnostic tool for monitoring multidrug resistance expression in human tumor tissues. Citations & References

  • Authors: Schwarzenbach H
  • Journal: Analytical Biochemistry
Catalog #
  • 4318739(Discontinued)
  • 4327059(Discontinued)
  • 4327058(Discontinued)

Chemical Compatibility Quick Reference Chart Product Literature

Primer extension analysis provides a sensitive tool for the identification of PCR-amplified DNA from HIV-1. Citations & References

  • Authors: Boni J; Schupbach J
  • Journal: Journal of Virological Methods
Catalog #

What can I do if the amplification of my target gene is later than expected for my qPCR experiment? Product FAQ

Answer

There are several reasons that amplification could be delayed. Please see the information in our Real-Time Troubleshooting Tool for more details (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/abnormal-amplification-curves/amplification-occurs-later.html).

Answer Id:: E7504

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