Manual / Product Insert

Protocol: NativeMark Unstained Protein Standard

Version: MAN0007920 Rev.B 18Mar2014
Catalog #

Manual / Product Insert

Protocol: HiMark Unstained Protein Standard

Version: MAN0009621 Rev.B 18Mar2014
Catalog #

Product FAQ

What are the storage conditions and shelf life for the Invitrogen Sharp Unstained Protein Standard?

Answer

We recommend storing the Invitrogen Sharp Unstained Protein Standard at -20 degrees C. It is stable for 12 months when properly stored.

Answer Id: E11693

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Manual / Product Insert

Protocol: Mark 12 Unstained Protein Standard

Version: MAN0007919 Rev. A.0 (18Mar2014)
Catalog #

Product FAQ

What are the molecular weights of the proteins in the NativeMark Unstained Protein Standard?

Answer

The NativeMark Unstained Protein Standard allows the estimation of molecular weight of proteins when performing native electrophoresis. It is suitable for use with NativePAGE Bis-Tris gels, or gradient Tris-Glycine and Tris-Acetate gels run using Tris-Glycine Native Running buffer. Here are the molecular weights of the proteins in the NativeMark Unstained Protein Standard:

Band 1: IgM hexamer; 1236 kDa in NativePAGE 3-12% Bis-Tris, NuPAGE 3-8% Tris-Acetate, and 4-12% Tris-Glycine gels
Band 2: IgM pentamer; 1048 kDa in NativePAGE 3-12% Bis-Tris, NativePAGE 4-16% Bis-Tris, NuPAGE 3-8% Tris-Acetate, and 4-12% Tris-Glycine gels
Band 3: Apoferritin band 1; 720 kDa in NativePAGE 3-12% Bis-Tris, NativePAGE 4-16% Bis-Tris, NuPAGE 3-8% Tris-Acetate, and 4-12% Tris-Glycine gels
Band 4: Apoferritin band 2; 480 kDa in NativePAGE 3-12% Bis-Tris, NativePAGE 4-16% Bis-Tris, NuPAGE 3-8% Tris-Acetate, and 4-12% Tris-Glycine gels
Band 5: β-phycoerythrin*; 242 kDa in NativePAGE 3-12% Bis-Tris, NativePAGE 4-16% Bis-Tris, NuPAGE 3-8% Tris-Acetate, and 4-12% Tris-Glycine gels
Band 6: Lactate dehydrogenase; 146 kDa in NativePAGE 3-12% Bis-Tris, NativePAGE 4-16% Bis-Tris, NuPAGE 3-8% Tris-Acetate, and 4-12% Tris-Glycine gels
Band 7: Bovine serum albumin; 66 kDa in NativePAGE 3-12% Bis-Tris, NativePAGE 4-16% Bis-Tris, and 4-12% Tris-Glycine gels
Band 8: Soybean trypsin inhibitor; 20 kDa in NativePAGE 3-12% Bis-Tris, NativePAGE 4-16% Bis-Tris, and 4-12% Tris-Glycine gels

*Note: The B-phycoerythrin band may appear pink on the gel as the protein has an intrinsic pink color and autofluoresces.

Answer Id: E11712

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Product FAQ

What are the origins of the proteins in the Invitrogen Sharp Unstained Protein Standard?

Answer

The proteins in the Invitrogen Sharp Unstained Protein Standard are synthetic fusion proteins and their origins are proprietary. The 10, 20, 60 and 80 kDa proteins in the Invitrogen Sharp Unstained Protein Standard have C-terminal 6xHis tags and hence are shown to cross react with the anti-His C-term antibody.

Answer Id: E11694

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Product FAQ

Can I use the NativeMark Unstained Protein Standard for denaturing electrophoresis applications?

Answer

The NativeMark Unstained Protein Standard is designed for use with native gels such as NativePAGE Bis-Tris gels, or gradient Tris-Glycine and Tris-Acetate gels run using Tris-Glycine Native Running buffer. Using this standard with denaturing gels will result in inaccurate molecular weight estimation.

Answer Id: E11714

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Manual / Product Insert

Protocol: Novex Sharp Unstained Protein Standard

Version: MAN0009622 Rev.B 18Mar2014
Catalog #

Product FAQ

What are the storage conditions and shelf life for the NativeMark Unstained Protein Standard?

Answer

We recommend storing the NativeMark Unstained Protein Standard at -20 degrees C in aliquots to avoid repeated freezing and thawing. After a vial is thawed for use, we recommend storing it at 4 degrees C for up to one month. It is stable for 6 months when stored at -20 degrees C.

Answer Id: E11711

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Product FAQ

What are the molecular weights of the proteins in the HiMark Unstained Protein Standard?

Answer

The HiMark Unstained Protein Standard allows the accurate estimation of molecular weight of high molecular weight proteins on NuPAGE Tris-Acetate Gels with Tris-Acetate SDS buffer system. Here are the molecular weights of the proteins in the HiMark Unstained Protein Standard:

- Band 1: 500 kDa
- Band 2: 290 kDa
- Band 3: 240 kDa
- Band 4: 160 kDa
- Band 5: 116 kDa
- Band 6: 97 kDa
- Band 7: 66 kDa
- Band 8: 55 kDa
- Band 9: 40 kDa

Answer Id: E11691

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Product FAQ

What are the storage conditions and shelf life for the HiMark Unstained Protein Standard?

Answer

We recommend storing the HiMark Unstained Protein Standard at -20 degrees C in aliquots to avoid repeated freeze thawing. The ladder is stable for 6 months when properly stored.

Answer Id: E11690

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Manual / Product Insert

E-PAGE MagicMark Unstained Protein Standard

Version: 17 January 2012
Catalog #
  • LC5701(Discontinued)

Product FAQ

What are the molecular weights of the proteins in the Invitrogen Sharp Unstained Protein Standard?

Answer

A wide range of molecular weight bands consisting of 12 proteins in the range of 3.5 - 260 kDa. Please see figure on this webpage (http://www.thermofisher.com/order/catalog/product/LC5801?ICID=search-product) showing the molecular weights of the proteins in the Invitrogen Sharp Unstained Protein Standard. These molecular weights apply to NuPAGE Bis-Tris, Tris-Glycine and Tricine gels. This standard has not been tested on NuPAGE Tris-Acetate gels. Note that the 3.5 kDa band is visible only on NuPAGE Bis-Tris gels run using 1X MES SDS Running buffer or Tricine gels. This band is also not visible when stained with SYPRO Ruby's microwave staining protocol.

Answer Id: E11696

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Product FAQ

Can I use the Invitrogen Sharp Unstained Protein Standard on zymogram gels?

Answer

The Invitrogen Sharp Unstained Protein Standard has not been tested on zymogram gels. We recommend using the SeeBlue Plus2 standard (at 2-3X concentration) for those gels.

Answer Id: E11695

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