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Midstream urine collection Product Literature

Peezy Midstream Product Overview Product Literature

Molecular Urine Collection & Transport Products Product Literature

AssayAssure Universal Urine Collection & Transport Products Product Literature

Brochure: Thermo Scientific Samco Containers and sample handling products Product Literature

How do I process urine samples for use on the Luminex assay platform? Product FAQ

Answer

Use only freshly collected urine samples. Dilute as recommended in the user guide provided with the kit. As needed, clarify samples by centrifugation (14,000 rpm for 10 mins) and/or filter them prior to analysis to prevent clogging of the filter plates and/or probe.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E12644

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Lot # specs Certificate

Catalog # 90111TS

Capitol Vial triple seal design Product Literature

What kind of samples have you worked with for the SMART Digest Trypsin Kit? Product FAQ

Answer

To date we have successful applications in mouse, monkey, beagle and human plasma samples. We also have successful applications in cell lysate, urine and cerebral spinal fluid samples.

Answer Id: E20936

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Thermo Scientific Samco Containers and Sample Handling Product Product Literature

Have you seen any effects from hemolysis influencing exosome isolation? Product FAQ

Answer

Overall, we have not encountered issues related to hemolysis. We would recommend using care with body fluid collection and preservation. For recommendations on standardization of sample collection, isolation, and analysis, please refer to the following reference: Witwer et al., Journal of Extracellular Vesicles (2013) 2:20360.

Answer Id: E7413

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Thermo Scientific Capitol Vial Drugs of Abuse Products [EN] Product Literature

Catalog: laboratory products consumables and small instruments (Japanese) Product Literature

After isolating exosomes using the Total Exosome Isolation reagent, how can I wash them? Product FAQ

Answer

The Total Exosome Isolation reagent precipitates exosomes by tying up water molecules and forcing them out of solution. Once the reagent is completely removed and PBS is added, exosomes will start going back into solution. Typically, no washing is needed. You can collect the remaining droplets of the reagent by addtiional quick centrifugation, and discard them. To do a wash, we would recommend performing a quick rinse in PBS by spinning for 5 minutes at 14,000 g at 4 degrees C. We do not recommend vortexing of the pellet.

Answer Id: E7434

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Can you isolate exosomes from embroyonic stem cell (ESC) culture medium? What type of media and culture conditions do you recommend? Product FAQ

Answer

While we have not tested ESC, for those media that we have tested, the number of secreted exosomes and isolation protocols are very similar. When growing cells, use chemically defined media or exosome-depleted FBS. Exosomes can be collected at 24 hours. 1-5 mL of media is a good starting point to isolate exosomes. This should be enough for several real-time PCR or Western blotting experiments. Sequencing and some other downstream analyses may require more starting material, and for this, we would recommend 30 mL cell culture medium to start.

Answer Id: E7415

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