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VEGF Receptor 2 (VEGF R2/VEGFA) Mouse ELISA Kit (Invitrogen™)

VEGF Receptor 2 (VEGF R2/VEGFA) Mouse ELISA Kit for ELISA

VEGF Receptor 1 (Flt1) Monoclonal Antibody (7A6), eBioscience™ (Invitrogen™)

VEGF Receptor 1 (Flt1) Monoclonal Antibody for Western Blot

VEGF-R1 Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human VEGF-R1 Simplex ProcartaPlex kit measures VEGF-R1 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Lab Vision™ Vascular Endothelial Growth Factor (VEGF) Ab-3, Mouse Monoclonal Antibody (Thermo Scientific™)

Provide accurate, reproducible results with the Thermo Scientific™ Vascular Endothelial Growth Factor (VEGF) Ab-3, Mouse Monoclonal Antibody.

VEGF Receptor 1 (Soluble) Human ELISA Kit (Invitrogen™)

VEGF Receptor 1 (Soluble) Human ELISA Kit for ELISA

VEGF Receptor 3 Monoclonal Antibody (AFL4), eBioscience™ (Invitrogen™)

VEGF Receptor 3 Monoclonal Antibody for Western Blot, IHC (F), Flow, IP, Neu, FN

Lab Vision™ Vascular Endothelial Growth Factor (VEGF) Ab-7, Mouse Monoclonal Antibody (Thermo Scientific™)

Provide accurate, reproducible results with the Thermo Scientific™ Vascular Endothelial Growth Factor (VEGF) Ab-7, Mouse Monoclonal Antibody.

VEGF Receptor 3/FLT4 Human ELISA Kit (Invitrogen™)

VEGF Receptor 3/FLT4 Human ELISA Kit for ELISA

VEGF-R2 Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human VEGF-R2 Simplex ProcartaPlex kit measures VEGF-R2 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Phospho-VEGF Receptor 2 (Tyr1054) Polyclonal Antibody (Invitrogen™)

Phospho-VEGF Receptor 2 (Tyr1054) Polyclonal Antibody for Western Blot

VEGF Receptor 2 Polyclonal Antibody (Invitrogen™)

VEGF Receptor 2 Polyclonal Antibody for Western Blot, IP

TaqMan™ Array, Human VEGF Pathway, Fast 96-well (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human VEGF Pathway 96-well Plate contains 44 assays to VEGF Pathway associated genes and 4 assays to candidate endogenous control genes. All assays are plated in duplicate.

VEGF Receptor 1 Polyclonal Antibody (Invitrogen™)

VEGF Receptor 1 Polyclonal Antibody for Western Blot, IF, ICC

Lab Vision™ Vascular Endothelial Growth Factor (VEGF), Rabbit Polyclonal Antibody (Thermo Scientific™)

Take advantage of one marker in a comprehensive antibody portfolio that addresses all major disease states with the Thermo Scientific™ Vascular Endothelial Growth Factor (VEGF), Rabbit Polyclonal Antibody.

EG-VEGF/PROK1 Human ELISA Kit (Invitrogen™)

EG-VEGF/PROK1 Human ELISA Kit for ELISA