Genomics and Proteomics


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Products

Genomics and proteomics search results

TSX Series High-Performance Undercounter Lab Refrigerators Thermo Scientific™

Designed for your environment, new Thermo Scientific™ TSX Series high-performance undercounter lab refrigerators support sample protection and sustainability objectives for the storage of media, reagents, chemotherapy, pharmaceutical and other research and medical products. Powered by V-drive, the TSX Series delivers low energy, decreased noise levels and improved performance and user experience.  These new undercounter refrigerators are quiet and feature an improved user interface, automated locking and increased storage capacity and reliability.

Diploid Growth Serum-Reduced Medium (SRM) Gibco™

The Gibco Diploid Serum-Reduced Medium (SRM) system is designed to support vaccine manufacture under serum-reduced or serum-free conditions for cell types traditionally cultured in classical media with 10% serum. With this system, human diploid cells, such as MRC-5, Wi38, and KMB17, can be grown with just 1–2% serum. Additionally, it supports growth of chicken embryo fibroblasts under serum-free conditions. Virus production can then be conducted under serum-free conditions without the addition of albumin or other supplements.

The Diploid SRM system is composed of two different kits:
1. Diploid Growth Serum-Reduced Medium (SRM) kit (this page): A basal medium and 100X growth supplement designed for cell expansion
2. Diploid Production Serum-Free Medium (SFM) kit: A basal medium and 100X production supplement designed for virus production

Human cells have traditionally been used to manufacture a variety of vaccines, such as the varicella zoster, polio, smallpox, hepatitis A, measles, mumps, and rubella vaccines. Traditionally, the only way to culture these cells has been to use classical media with 10% serum for growth and 1–5% human albumin (human sourced or recombinant) for virus production. These are two of the highest cost components in vaccine production. The Diploid SRM system helps reduce or eliminate both components while maintaining high virus titers.

Cost savings
Reduction or elimination of serum can significantly reduce cost for a vaccine manufacturing process. Premium FBS is expensive with high price volatility. Given the typical cost pressures on vaccine production, both economic drivers make vaccine manufacturing to a target price extremely challenging. Diploid Growth SRM reduces the impact of this volatility in price and offers a level of cost consistency not available with processes that use serum in high volumes.

In addition, serum requires frozen storage. A 10% serum concentration in growth media can result in high frozen storage requirements for a vaccine process. A process requiring 1,000 L of growth medium would require the frozen storage of 100 L of serum. The Diploid Growth SRM kit comes with a 100X frozen supplement, enabling an 80% reduction in frozen storage requirements. 1,000 L of Diploid Growth SRM would only need 20 L of frozen storage: 10 L for FBS and 10 L for the 100X supplement.

Albumin is commonly added to virus production media. With the Diploid Production SFM, viruses can be produced at comparable titers without any additional supplementation.

No adaptation required
The Diploid SRM system was developed with existing cell banks in mind. Transitioning a vaccine process to a new medium can be challenging due to the need to adapt the cells to the new medium. For human cells such as MRC-5, there is the added risk of reaching the Hayflick limit due to the added passages necessary during cell adaptation and subsequent re-banking of the cells. The transition to the Diploid SRM system does not require adaptation or re-banking of cells when using the Diploid Growth SRM kit. Existing cGMP cells banked with serum may be thawed directly in Diploid Growth SRM supplemented with 1% FBS, allowing a simple transition to this medium.

Mitigate serum supply risk
Obtaining a steady supply of serum can be challenging, especially if the serum required is from a BSE/TSE safe region such as New Zealand or Australia. Serum supply can fluctuate based on several environmental (drought, cattle disease) and economic (feed cost, dairy pricing) factors. With Diploid Growth SRM this dependence is substantially reduced due to the reduced serum requirements.

Diploid Production Serum-Free Medium (SFM) Gibco™

The Gibco Diploid Serum-Reduced Medium (SRM) system is designed to support vaccine manufacture under serum-reduced or serum-free conditions for cell types traditionally cultured in classical media with 10% serum. With this system, human diploid cells, such as MRC-5, Wi38, and KMB17, can be grown with just 1–2% serum. Additionally, it supports growth of chicken embryo fibroblasts under serum-free conditions. Virus production can then be conducted under serum-free conditions without the addition of albumin or other supplements.

The Diploid SRM system is composed of two different kits:
1. Diploid Growth Serum-Reduced Medium (SRM) kit: A basal medium and 100X growth supplement designed for cell expansion
2. Diploid Production Serum-Free Medium (SFM) kit (this page): A basal medium and 100X production supplement designed for virus production

Human cells have traditionally been used to manufacture a variety of vaccines, such as the varicella zoster, polio, smallpox, hepatitis A, measles, mumps, and rubella vaccines. Traditionally, the only way to culture these cells has been to use classical media with 10% serum for growth and 1–5% human albumin (human sourced or recombinant) for virus production. These are two of the highest cost components in vaccine production. The Diploid SRM system helps reduce or eliminate both components while maintaining high virus titers.

Cost savings
Reduction or elimination of serum can significantly reduce cost for a vaccine manufacturing process. Premium FBS is expensive with high price volatility. Given the typical cost pressures on vaccine production, both economic drivers make vaccine manufacturing to a target price extremely challenging. Diploid Growth SRM reduces the impact of this volatility in price and offers a level of cost consistency not available with processes that use serum in high volumes.

In addition, serum requires frozen storage. A 10% serum concentration in growth media can result in high frozen storage requirements for a vaccine process. A process requiring 1,000 L of growth medium would require the frozen storage of 100 L of serum. The Diploid Growth SRM kit comes with a 100X frozen supplement, enabling an 80% reduction in frozen storage requirements. 1,000 L of Diploid Growth SRM would only need 20 L of frozen storage: 10 L for FBS and 10 L for the 100X supplement.

Albumin is commonly added to virus production media. With the Diploid Production SFM, viruses can be produced at comparable titers without any additional supplementation.

No adaptation required
The Diploid SRM system was developed with existing cell banks in mind. Transitioning a vaccine process to a new medium can be challenging due to the need to adapt the cells to the new medium. For human cells such as MRC-5, there is the added risk of reaching the Hayflick limit due to the added passages necessary during cell adaptation and subsequent re-banking of the cells. The transition to the Diploid SRM system does not require adaptation or re-banking of cells when using the Diploid Growth SRM kit. Existing cGMP cells banked with serum may be thawed directly in Diploid Growth SRM supplemented with 1% FBS, allowing a simple transition to this medium.

Mitigate serum supply risk
Obtaining a steady supply of serum can be challenging, especially if the serum required is from a BSE/TSE safe region such as New Zealand or Australia. Serum supply can fluctuate based on several environmental (drought, cattle disease) and economic (feed cost, dairy pricing) factors. With Diploid Growth SRM this dependence is substantially reduced due to the reduced serum requirements.

Nunc™ Polystyrene Roller Bottles Thermo Scientific™

Grow cells with high reproducibility by using highly-reliable Thermo Scientific™ Nunc™ Polystyrene Roller Bottles. Developed for applications such as the industrial scale production of vaccines, monoclonal antibodies and biotherapeutics. Nunc PS Roller Bottles are sterilized by gamma irradiation and meet sterility assurance level (SAL) 10-6

Calf Serum with Iron (US Origin) Gibco™

Gibco Calf Serum with Iron (US Origin) is collected from calves 1-6 months of age, which naturally have higher levels of transferrin to bind iron. The superior levels of transferrin promote higher performance in cell culture applications.

Key features:
• Iron content: 500–700 µg/dL
• Triple 0.1-micron sterile filtered
• Cost effective
• US sourced

POROS™ Oligo (dT)25 Affinity Resin Thermo Scientific™

POROS Oligo (dT)25 Affinity Resin is designed for the purification and isolation of mRNA from in vitro transcription (IVT) manufacturing processes. The resin effectively separates mRNA from components of the transcription reaction process, such as enzymes and plasmid DNA.

POROS Oligo (dT)25 Affinity Resin was developed to address the selectivity and capacity requirements for large-scale downstream purification of a range of mRNA constructs used in vaccine and gene therapy applications. Unlike alternate approaches, the resin selectively captures the mRNA via the polyadenylated (polyA) tail using simple salt and water purification steps.

Features of POROS Oligo (dT)25 Affinity Resin include:
• Easy mRNA purification from crude transcription mix to effectively remove plasmid DNA and other IVT components
• Simplified workflow helps to maximize efficiency, thereby reducing complexity of subsequent polish steps
• Excellent scalability
• Non-animal derived

POROS Oligo (dT)25 Affinity Resin allows for a robust platform and efficient purification process for a range of mRNA molecules and applications.

Pack sizes of 1 L and above come with a regulatory support package. Worldwide technical support is also available.

Main characteristics

Matrix: cross-linked poly(styrene-divinylbenzene) POROS beads
Average particle size: 50 µm
Ligand: poly(dT) 25mer with proprietary linker
Binding capacity: >2 mg mRNA per mL of resin
Binding conditions: 0.5–1 M salt, common buffers, neutral pH
Elution conditions: Low salt, common buffers, neutral pH
Mechanical resistance: 100 bar (1,450 psi; 10 MPa)
Thermal stability: allows sample denaturing at 65°C if needed
Formulation buffer: 18% (v/v) ethanol

Oligo (dT) ligands are manufactured using a synthetic manufacturing process that are free of animal components.

Peptone Preview Pak Gibco™

The Peptone Preview Pak features our new pea, wheat, cotton, soy, and yeast peptones and provides unique nutritional profiles compared to other cell culture supplements.

• Easy-to-test pack of animal origin-free peptones
• Unique nutritional profiles
• Cell culture supplements

The Peptone Preview Pak contains:
• Wheat 100, UF—an ultra-filtered supplement and good source of glutamine, which makes it ideal for mammalian cell culture applications. It is digested for enhanced peptide content resulting in a low concentration of free amino acids and carbohydrates.
• Cotton 100, UF—rich in nucleoside content and digested to enhance peptide content. It is processed to provide higher concentrations of vitamins, which can help maintain performance in mammalian and microbial processes.
• Cotton 200, UF—ultra-filtered and rich in nucleoside content, processed to enhance carbohydrate concentrations, which is important for some cell culture and microbial processes
• Soy 100—a highly digested soy protein peptone that is high in nucleosides and free amino acid content. It has high concentrations of vitamins and carbohydrates and is recommend for a wide variety of processes.
• Pea 100, UF—an ultra-filtered peptone digested to enhance nucleoside and free amino acid content. The high total amino acid content and low carbohydrate content are similar to many high-performance animal origin peptones.
• Yeast 100—designed to enhance the content of vitamins and other key nutritional components found in yeast. It is intended for nutritional supplementation for mammalian, microbial, and insect cell culture.

Peptone Preview Pak supplements are non-GMP prototype products that are not for manufacture use. Larger sizes are available through the Gibco Custom Products Services.

Yeast Extract Gibco™

Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells. They are derived from primary grown baker’s yeast. These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications. Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation.

Related yeast extract products:
• Bacto Yeast Extract—one of the most complete and versatile fermentation supplements available. It is an important ingredient for the microbiological assay of vitamins. Yeast extract is also of value in the assay of antibiotics. B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract.
• Bacto Yeast Extract, Technical—developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics
• Gibco Yeast Extract—developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Difco Yeast Extract UF—ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g.
• Difco Yeast Extract Low-Dusting (LD)—designed to limit the amount of dust generated compared to other spray-dried yeast extracts.

IgA Human Uncoated ELISA Kit with Plates Invitrogen™

IgA Human Uncoated ELISA Kit with Plates for ELISA

resDNASEQ™ Quantitative Vero DNA Kit with PrepSEQ™ Residual DNA Sample Preparation Kit Applied Biosystems™

The resDNASEQ® Quantitative Vero DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Vero (African Green Monkey) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of Vero cell DNA, typically in less than four hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high molecular weight or sheared DNA.

This kit is bundled together with sample preparation reagents in the form of a PrepSEQ® Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts. For the quantitation kit alone without the sample preparation kit, please see resDNASEQ® Quantitative Vero DNA Kit.

• Highly sensitive quantitation using proven TaqMan® real-time qPCR technology
• Offers consistent performance across the expected range of DNA fragment sizes
• Integrated system includes sample preparation kit, master mix, TaqMan® primer/probe mix, and Vero genomic DNA standard

The resDNASEQ® Quantitative Vero DNA Kit is designed to provide highly sensitive detection of Vero DNA, allowing you to use small sample volumes to help generate accurate results. The broad linear range of TaqMan® real-time qPCR technology allows you to test samples with variable levels of Vero DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts.

Easy to use, results in under 4 hours
The easy workflow of the resDNASEQ® Quantitative Vero DNA Kit consists of sample preparation, assay setup, and instrument run and data analysis—all of which you can perform typically in under 4 hours. Start by extracting DNA from your in-process purification and drug substance samples. Then perform qPCR analysis to compare the amount of Vero DNA in your test samples to a standard curve generated with known amounts of purified Vero genomic DNA standard. The resDNASEQ® system includes almost everything you need to carry out these steps, from sample preparation kit to master mix to TaqMan® primer/probe mix to Vero genomic DNA standard, as well as step-by-step instructions.

Consistent performance even with fragmented DNA
For accurate quantitation of residual Vero DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ® system you get consistent assay performance across a range of DNA fragment sizes.

IgA Human Uncoated ELISA Kit Invitrogen™

IgA Human Uncoated ELISA Kit for ELISA

resDNASEQ™ Quantitative Vero DNA Kit Applied Biosystems™

The resDNASEQ® Quantitative Vero DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Vero (African Green Monkey) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of Vero cell DNA, typically in less than four hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high molecular weight or sheared DNA.

This kit does not include sample preparation reagents—for this, please see resDNASEQ® Quantitative Vero DNA Kit with PrepSEQ® Residual DNA Sample Preparation Kit.

• Highly sensitive quantitation using proven TaqMan® real-time qPCR technology
• Offers consistent performance across the expected range of DNA fragment sizes
• Integrated system includes sample preparation kit, master mix, TaqMan® primer/probe mix, and Vero genomic DNA standard
• Manual and automated sample preparation, optimized for quantitative recovery from complex sample matrices

The resDNASEQ® Quantitative Vero DNA Kit is designed to provide highly sensitive detection of Vero DNA, allowing you to use small sample volumes to help generate accurate results. The broad linear range of TaqMan® real-time qPCR technology allows you to test samples with variable levels of Vero DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts.

Easy to use, results in under 4 hours
The easy workflow of the resDNASEQ® Quantitative Vero DNA Kit consists of sample preparation, assay setup, and instrument run and data analysis—all of which you can perform typically in under 4 hours. Start by extracting DNA from your in-process purification and drug substance samples. Then perform qPCR analysis to compare the amount of Vero DNA in your test samples to a standard curve generated with known amounts of purified Vero genomic DNA standard. The resDNASEQ® system includes almost everything you need to carry out these steps, from sample preparation kit to master mix to TaqMan® primer/probe mix to Vero genomic DNA standard, as well as step-by-step instructions.

Consistent performance even with fragmented DNA
For accurate quantitation of residual Vero DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ® system you get consistent assay performance across a range of DNA fragment sizes.

Resurge™ CD3 Supplement, without glucose and L-glutamine Gibco™

Resurge CD3 Supplement, without glucose and L-glutamine, is an animal origin-free (AOF) protein-free chemically defined supplement. It can be used in batch and fed batch mode in cell culture processes. All components are chemically defined with known molecular formulae and structures and meet our stringent Animal Origin-Free (AOF) specification.

Resurge CD3 Supplement is formulated without glucose, L-glutamine, and sodium bicarbonate. Due to the balanced nutritional nature of this supplement, rich in amino acids, it has also been used in bacterial applications.

Features:
• Animal origin-free
• Chemically defined
• Protein-free
• Rich in amino acids

Applications of the Resurge CD3 Supplement, without glucose and L-glutamine, include:
• Can be used in batch and fed batch mode in cell culture processes
• Use in bacterial applications

Bacto™ Proteose Peptone No. 3 Gibco™

Bacto Proteose Peptone No. 3 is a member of the Bacto Proteose Peptones, which are enzymatic digests of protein. Studies of peptic digests of animal tissue prepared under varying digestion parameters demonstrated that no single peptone is the most suitable nitrogen source for every microbiological application. These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone.

• Bacto Proteose Peptone is used in preparing microbiological culture media and in producing bacterial toxins
• Bacto Proteose Peptone No. 2 is used in preparing microbiological culture media
• Bacto Proteose Peptone No. 3 is used in preparing microbiological and mammalian cell culture media
• Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone. It offers the same beneficial nutrients as Bacto Proteose Peptone for growth promotion and toxin production in a wide range of fastidious microorganisms.

We also offer alternatives to Bacto Proteose Peptones for scale-up to production applications, including BiTek Proteose Peptone.

Sf21 cells in Sf-900™ III SFM Gibco™

Gibco® Sf21 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Gibco® Sf21 cells are adapted to serum-free suspension culture in Gibco® Sf-900™ III SFM, which saves significant time and expense associated with the adaptation of cultures. Gibco® Sf21 cells (frozen in Gibco® Sf-900™ III SFM) feature:
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Quality and performance testing

Recombinant protein expression from a variety of expression systems
High levels of protein expression in Sf21 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.

Good growth in adherent or suspension culture
Each vial containing 1 × 107 cells can be thawed directly into suspension or adherent culture. Protocols for adherent and suspension growth in Gibco® Sf-900™ III SFM are available in the product manual. Cultures can easily be transferred between the two conditions for ease of workflow but we recommend the stock cultures are maintained in suspension.

Quality and performance testing
Each lot of Gibco® Sf21 cells is tested for cell growth and viability post-recovery from cryopreservation.

Product Use
For Research Use Only. Not for any animal or human therapeutic or diagnostic use. Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.
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