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Virological evaluation of domestic water purification devices commonly used in India emphasizes inadequate quality and need for virological standards Citations & References

  • Authors: Verma, V; Arankalle, VA
  • Journal: TROPICAL MEDICINE & INTERNATIONAL HEALTH
Catalog #
  • 4339386
  • 4413750
  • N8050400(Discontinued)
  • N8050200(Discontinued)
  • N8050002(Discontinued)
  • ZGEXSC9700384W
  • ZGEXSC9700384W3Y
  • N8050002R
  • 4441166

I am using the BenchPro 2100 Plasmid Purification System. Can I elute the DNA in less TE? Can I use water instead of TE? Product FAQ

Answer

Yes, you can replace TE with water. Carefully remove all TE solution from the TE-containing well (the one closest to the elution tube slot on the reagent tray), rinse the well twice with purified water and then fill with 1.5 mL purified water. You can also pipette out up to 700 µL of TE, so that the final DNA concentration could be higher. We would not recommend using less than 750 µL TE buffer.

Answer Id:: E7760

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Can I use a frozen cell pellet with the BenchPro 2100 instrument? Product FAQ

Answer

Yes. You can resuspend the pellet in LB media or sterile water such that the OD at 600 nm is 2.0.

Answer Id:: E7783

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An immunomagnetic separation-real-time PCR method for quantification of Cryptosporidium parvum in water samples. Citations & References

  • Authors: Fontaine M; Guillot E
  • Journal: Journal of Microbiological Methods
Catalog #
  • 4318739(Discontinued)
  • 4327059(Discontinued)
  • 4327058(Discontinued)

Microfluidic isolation of leukocytes from whole blood for phenotype and gene expression analysis. Citations & References

  • Authors: Sethu P, Moldawer LL, Mindrinos MN, Scumpia PO, Tannahill CL, Wilhelmy J, Efron PA, Brownstein BH, Tompkins RG, Toner M
  • Journal: Anal Chem
  • PubMed ID: 16878882

My BenchPro 2100 instrument failed several minutes before the end of the run, and I got this error message: "PROTOCOL ABORTED Inlet pressure out of range". Why is this? Product FAQ

Answer

This is usually caused by a sudden dip in the air flow (an air system problem, usually not related to the instrument itself). During the BenchPro 2100 procedure, the lysis step and elution step are the two steps that require higher air pressure, so this error message is usually seen at these two steps. If this error happens during the lysis step, we recommend restarting from the beginning, with a new culture, new card, and new reagent tray. There is no way to recover the sample.

If this happens at the elution step, you can see if you can get any eluted DNA. Hopefully some DNA has already eluted out (usually most DNA is eluted out in the first 1-2 minutes of the elution step). If this happens right before the elution step, with no solution in the elution tube yet, you can empty out the reagent tray reservoirs and refill them with 1.5 ml of TE or water in the right reservoir, then set up the run with the same tray and card and run the protocol again.

Answer Id:: E7802

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High density DNA data storage library via dehydration with digital microfluidic retrieval. Citations & References

  • Authors: Newman S, Stephenson AP, Willsey M, Nguyen BH, Takahashi CN, Strauss K, Ceze L,
  • Journal: Nat Commun
  • PubMed ID: 30979873
Catalog # Q33238

Passively driven integrated microfluidic system for separation of motile sperm. Citations & References

  • Authors: Cho BS, Schuster TG, Zhu X, Chang D, Smith GD, Takayama S
  • Journal: Anal Chem
  • PubMed ID: 12705601
Catalog #

Extended application of flow microfluorometry by means of dual laser excitation. Citations & References

  • Authors: Stöhr M, Eipel H, Goerttler K, Vogt-Schaden M
  • Journal: Histochemistry
  • PubMed ID: 870462

In vitro model of the outer blood-retina barrier. Citations & References

  • Authors: Steuer H, Jaworski A, Stoll D, Schlosshauer B
  • Journal: Brain Res Brain Res Protoc
  • PubMed ID: 15063838

Dynabeads® Oligo (dT)25-61002 Experiment Protocol

Dynabeads® Oligo (dT)25-61005 Experiment Protocol

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