Product FAQ

I am observing poor specific signal in my gel stained with Pro-Q Diamond Phosphoprotein Gel Stain, or also observe a weak total protein staining pattern when I switch to a different excitation or emission filter. What is causing this?

Answer

Many total protein stains including SYPRO Ruby Gel Stain and Coomassie Blue stain will quench the Pro-Q Diamond signal. If you are staining your gels or blots with Pro-Q Diamond stain in containers that have previously been used for a total protein stain, you may be contaminating your gel with residue left on the staining dish from the total protein stain. Either use new containers, such as plastic weigh boats, designated containers for each stain, or rinse the container well in ethanol and wipe out any residual residue with a Kimwipe tissue.

Answer Id: E11296

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Product FAQ

I am observing poor specific signal in my gel stained with Pro-Q Emerald 300/488 Glycoprotein Gel Stain, or also observe a weak total protein staining pattern when I switch to a different excitation or emission filter. What is causing this?

Answer

Many total protein stains including SYPRO Ruby Gel Stain and Coomassie Blue stain will quench the Pro-Q Emerald signal. If you are staining your gels or blots with Pro-Q Emerald stain in containers that have previously been used for a total protein stain, you may be contaminating your gel with residue left on the staining dish from the total protein stain. Either use new containers, such as plastic weigh boats, designated containers for each stain, or rinse the container well in ethanol and wipe out any residual residue with a Kimwipe tissue.

Answer Id: E11300

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