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Find the right set of products that can assist you with detection and analysis of your protein. Find a comprehensive suite of solutions for every step of the western blotting workflow in order to help you obtain high-quality, publishable results with minimal time and effort.

(See a list of the products featured in this article.) Signaling pathways control and coordinate all aspects of cell function. Dissecting these pathways leads to the identification of key receptors, enzymes, and signaling molecules that regulate both normal development and disease states.

The term "blotting" refers to the transfer of biological samples from a gel to a membrane and their subsequent detection on the surface of the membrane. A western blot experiment, or western blotting (also called immunoblotting, because an antibody is used to specifically detect its antigen) was...

Chemiluminescent detection for western blotting is popular because it offers several advantages over other detection methods. Using chemiluminescence allows multiple exposures to be made, which enables optimization of signal to noise.

Unlike chemiluminescent or fluorescent blotting applications, chromogenic substrates do not require special equipment for visualization of the assay results. We offer several chromogenic substrates for your different experimental needs.

Protein transfer is a vital step in western blot analysis which involves the transfer of proteins separated in a gel by electrophoresis to a solid support matrix. Immobilizing the protein to a solid support matrix facilitates the detection of specific proteins using antibodies directed against the...

With a variety of detection techniques for western blotting-chemiluminescence, fluorescence, or colorimetric, to choose from, you can select a technology to match your experimental requirements. Chemiluminescent Detection Technical Tip Fluorescent Detection Technical Tip, Protein Electrophoresis and...

Introduction Chromogenic horseradish peroxidase western blot substrates Chromogenic alkaline phosphatase western blot substrates, Chromogenic Western Blot Detection Antibodies for Western Blotting Chloronaphthol Substrate Powder CN/DAB Substrate Kit 1-Step TMB-Blotting Substrate Solution 1-Step...

Obtain answers to your questions about one-dimensional (1D) protein gel electrophoresis using our precast gel systems. Get help with setting up your experiment and find tips and tricks to obtain optimal resolution of your protein.

We offer a wide range of reagents, kits, gels, membranes, antibodies, and equipment to facilitate every step of western blot analysis. Use the BlotBuilder selection tool to choose the appropriate set of products for your western blotting needs.

Thermo Scientific Pierce Fast Western Blot Kits accelerate the Western blotting process with streamlined protocols and optimized reagents that enables accuracy, sensitivity and reliability. The kits help reduce hands-on and overall blotting time to approximately one hour using any of the Thermo...

Multiplex fluorescent western blotting provides accurate, quantitative results, stable signals, and the ability to clearly evaluate multiple protein targets on a single blot, which makes this technique increasingly popular.

Our specialized products, innovative technologies and customized workflow solutions help expand and compliment the portfolios of life science tool providers., PCR & RT-PCR enzymes DNA/RNA modifying enzymes Restriction enzymes Molecular cloning enzymes Lyo-ready enzymes Enzyme engineering and...

Reliable transfer of high molecular weight (HMW) proteins (i.e., >150 kDa) from a gel to membrane during western blotting is a common challenge. Many factors affect the efficiency at which HMW proteins transfer.

Western blotting is a multi-step process that can take hours or one to two days to complete, depending on the methods used. Below are videos that detail the common steps used to perform a western blot, from separating proteins in protein gel electrophoresis to developing the blot.