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Has the BenchPro 4100 Card Processing Station been discontinued? Product FAQ

Answer

Yes, the BenchPro 4100 Card Processing Station has been discontinued as of December 31, 2014. The current BenchPro 4100 Western Cards (https://www.thermofisher.com/order/catalog/product/WP1001) and Reagent Vials (https://www.thermofisher.com/order/catalog/product/WP3001) will continue to be available for purchase. We offer the new iBind Western Device (http://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/western-blotting/western-blot-detection/ibind-western-system.html) as an alternative system for automated western blot processing.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Answer Id: E10882

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Products for Western Blotting Product Literature

Can I use fluorescent secondary antibodies for western blots? Product FAQ

Answer

Yes, as long as the instrument used to analyze the blots provides the appropriate excitation wavelengths and emission filter sets to detect fluorescence of the selected dye(s). The limits of detection are dependent upon the amount of protein/peptide per spot/band, using the correct excitation and emission settings for the dye-conjugated antibody, and the sensitivity of the camera.
Another alternative to dye-conjugated secondary antibodies are the quantum dot-conjugated antibodies provided in our WesternDot products. For more information: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/fluorescent-western-blot-detection/westerndot-fluorescent-immunodetection-kits.html

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E18039

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Can I use eBioscience antibodies for immunoblotting (WB)? Product FAQ

Answer

We do not routinely test our antibodies in house by western blotting (WB) unless it was specifically developed for that purpose; therefore, we rely on the literature as the basis for reporting WB as an approved application for a given clone. If a product is suitable for WB, it will be noted under reported applications on the products technical data sheet. Please refer to the Product Technical Datasheet for more information.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E14508

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If I want to strip and re-probe a nitrocellulose membrane, what procedure do you recommend? Product FAQ

Answer

Thermo Fisher Scientific sells 4 ready-to-use buffers specifically intended for stripping Western blots prior to re-probing. Three of them can be used with both nitocellulose and PVDF membranes. We have Restore Western Blot Stripping Buffer (Cat. No. 21059), a trial size of this product (Cat. No. 21062), a product called Restore PLUS (Cat. No. 46428), which is formulated to strip off antibodies that are difficult to remove, and Restore Fluorescent Western Blot Stripping Buffer (Cat. No. 62299). The latter is used to strip fluorophore-labeled antibodies from PVDF membranes only.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E5217

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Product Guide: Self Training Kit Western Blotting (Japanese) Product Literature

Article: Biological validation of a novel process and product for quantitating western blots Product Literature

Can I use SuperSignal West Pico PLUS Chemiluminescent Substrate with the identical protocol (i.e., same dilutions) I used with the GE Healthcare ECL Substrate or Thermo Scientific ECL Western Blotting Subtrate (Cat. No. 32106)? Product FAQ

Answer

No, SuperSignal substrates require different dilutions and 5-minute incubation on the blot. You must use less (more dilute) primary and secondary antibodies with SuperSignal West Pico PLUS Chemiluminescent Substrate (see product instructions for recommended ranges). Please see Tech Tip # 67: Chemiluminescent Western Blotting Technical Guide and Protocols (https://tools.thermofisher.com/content/sfs/brochures/TR0067-Chemi-Western-guide.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Answer Id: E8519

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What are the specifications for the BenchPro 4100 fuse, and can I purchase replacement fuses separately? Product FAQ

Answer

The fuse is 250 V, 1.25 A. We do not offer it as a standalone product, but it can be purchased from electronics vendors such as RadioShack.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Answer Id: E10897

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What are the product specifications for the PowerEase 300W Power Supply? Product FAQ

Answer

The product specifications for the PowerEase 300W Power Supply are listed on Page 18 of the manual (https://tools.thermofisher.com/content/sfs/manuals/PowerEase300W_UG_man.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Answer Id: E10950

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Can I purchase the WesternDot Qdot 625 streptavidin conjugate provided in the WesternDot 625 Goat Anti-Mouse Western Blot Kit (Cat. No. W10132) and WesternDot 625 Goat Anti-Rabbit Western Blot Kit (Cat. No. W10142) as a standalone product? Product FAQ

Answer

Yes, it can be purchased as Qdot 625 Streptavidin Conjugate (Cat. No. Q22063, 50 µL or Cat. No. A10196, 200 µL).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Answer Id: E10847

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Do E-PAGE gels contain a preservative? Product FAQ

Answer

E-PAGE gels do not contain a preservative. They undergo UV sterilization during the production process.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Answer Id: E10759

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Can I purchase the Reagent Vials and Reagent Bottles (Sets 1 and 2) for the BenchPro 4100 Card Processing Station separately? Product FAQ

Answer

The 25 mL Reagent Vials can be purchased separately as a set of 50 vials/package (Cat. No. WP3001). However, we do not offer the Reagent Bottles as standalone products. You may purchase them from Cole Parmer:

- 250 mL Reagent Bottles (Set 1) (Cat. No. EW-06019-76) (http://www.coleparmer.com/Product/Bottles_Squre_HDPE_250ml_10_pk/EW-06019-76

- 125 mL Reagent Bottles (Set 2) (Cat. No. EW-06254-20) (http://www.coleparmer.com/Product/Thermo_Scientific_Nalgene_Sterile_PETG_Media_Bottles_125_mL_48_box/EW-06254-20)

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Answer Id: E10884

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What is the concentration of protein in Human FABP3 Recombinant Protein (Cat. No. RP75543)? Product FAQ

Answer

The concentration of protein in Human FABP3 Recombinant Protein (Cat. No. RP75543) is lot-specific. This information can be found on the Certificate of Analysis which can be searched for on the product page.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Answer Id: E21529

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I used the SuperSignal West Pico PLUS Chemiluminescent Substrate. Why is the entire film black or showing a reversed image? Product FAQ

Answer

The antibody concentration (primary and secondary) is too high. Use the antibody dilutions described in the product instructions. Most background will disappear when the proper blocking reagent and a HRP conjugate concentration are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Answer Id: E8528

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