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We offer a wide range of reagents, kits, gels, membranes, antibodies, and equipment to facilitate every step of western blot analysis. Use the BlotBuilder selection tool to choose the appropriate set of products for your western blotting needs.

Quick visualization, precise quantitation, single-probe detection or multiplexing — we offer a range of reagents, kits and instruments for western blot detection and analysis., Optimized buffers and reagents for every step of the western blot process.

Find the right set of products that can assist you with detection and analysis of your protein. Find a comprehensive suite of solutions for every step of the western blotting workflow in order to help you obtain high-quality, publishable results with minimal time and effort.

The term "blotting" refers to the transfer of biological samples from a gel to a membrane and their subsequent detection on the surface of the membrane. A western blot experiment, or western blotting (also called immunoblotting, because an antibody is used to specifically detect its antigen) was...

(See a list of the products featured in this article.) The breakneck pace of cell biology research demands more sensitive and quantitative protein analysis methods, capable of dissecting increasingly complex protein expression and interaction patterns.

The right antibodies are essential for clean, definitive, and reproducible western blot results. Use the tabs below to find information on aspects to consider when selecting primary and secondary antibodies for western blot experiments.

Fluorescent western blotting is growing in popularity because it allows the ability to perform multiplex detection, where multiple proteins can be detected at the same time. Historically, the instrumentation available for fluorescent detection was not able to offer the sensitivity required by many...

Our specialized products, innovative technologies and customized workflow solutions help expand and compliment the portfolios of life science tool providers., PCR & RT-PCR enzymes DNA/RNA modifying enzymes Restriction enzymes Molecular cloning enzymes Lyo-ready enzymes Enzyme engineering and...

For Research Use Only. Not for use in diagnostic procedures., View all Is there a wet transfer blot module available for the Mini Gel Tank? Are Bolt Bis-Tris Plus gels compatible with other transfer methods? How many Mini Blot Modules can be run per Bolt Mini Gel Tank?

Chemiluminescent detection for western blotting is popular because it offers several advantages over other detection methods. Using chemiluminescence allows multiple exposures to be made, which enables optimization of signal to noise.

Unlike chemiluminescent or fluorescent blotting applications, chromogenic substrates do not require special equipment for visualization of the assay results. We offer several chromogenic substrates for your different experimental needs.

Studying protein–protein interactions by far-western blotting Critical factors of far–western blot analysis Recommended reading, Pierce Far-Western Blot Kit for Biotinylated Proteins Western Blotting Protein Gel Electrophoresis, The far–western blot technique is similar to western blotting; in a...

As with other components in a western blotting system, there are many chemiluminescent western blotting substrate choices available. The appropriate selection of a substrate depends on the detection level (sensitivity) required, the target protein's abundance, the sample abundance and antibody...

Fluorescent western blotting provides accurate, quantitative, stable signals, and the ability to clearly evaluate multiple protein targets on a single blot. We offer optimized reagents, buffers and fluorescent conjugated antibodies so you can get the most from your fluorescent western blots.

Thermo Scientific SuperSignal ECL substrates are HRP substrates that offer excellent performance in western blotting applications, with longer light emission and stronger signal intensity than other luminol-based or acridan-based detection systems.