We offer a wide range of reagents, kits, gels, membranes, antibodies, and equipment to facilitate every step of western blot analysis. Use the BlotBuilder selection tool to choose the appropriate set of products for your western blotting needs.
Learn More Western Blotting
Find the right set of products that can assist you with detection and analysis of your protein. Find a comprehensive suite of solutions for every step of the western blotting workflow in order to help you obtain high-quality, publishable results with minimal time and effort.
Learn More Overview of Western Blotting
The term "blotting" refers to the transfer of biological samples from a gel to a membrane and their subsequent detection on the surface of the membrane. A western blot experiment, or western blotting (also called immunoblotting, because an antibody is used to specifically detect its antigen) was...
(See a list of the products featured in this article.) The breakneck pace of cell biology research demands more sensitive and quantitative protein analysis methods, capable of dissecting increasingly complex protein expression and interaction patterns.
Learn More Western Blot Buffers
Learn about our specially formulated buffers for every step of western blot processing and detection. We offer a wide range of blocking buffers, wash buffers, detergents, and western blot stripping buffers to help generate the best data possible from your western blots.
Learn More Life Science Tools for Commercial Supply
Our specialized products, innovative technologies and customized workflow solutions help expand and compliment the portfolios of life science tool providers., PCR & RT-PCR enzymes DNA/RNA modifying enzymes Restriction enzymes Molecular cloning enzymes Lyo-ready enzymes Enzyme engineering and...
Learn More Far-Western Blot Analysis
Studying protein–protein interactions by far-western blotting Critical factors of far–western blot analysis Recommended reading, Pierce Far-Western Blot Kit for Biotinylated Proteins Western Blotting Protein Gel Electrophoresis, The far–western blot technique is similar to western blotting; in a...
Learn More Fluorescent Western Blot Detection
Fluorescent western blotting provides accurate, quantitative, stable signals, and the ability to clearly evaluate multiple protein targets on a single blot. We offer optimized reagents, buffers and fluorescent conjugated antibodies so you can get the most from your fluorescent western blots.
Learn More Chemiluminescent Western Blot Detection
As with other components in a western blotting system, there are many chemiluminescent western blotting substrate choices available. The appropriate selection of a substrate depends on the detection level (sensitivity) required, the target protein's abundance, the sample abundance and antibody...
Learn More Chromogenic Western Blot Detection
Unlike chemiluminescent or fluorescent blotting applications, chromogenic substrates do not require special equipment for visualization of the assay results. We offer several chromogenic substrates for your different experimental needs.
Learn More ECL Western Blot Substrates
Thermo Scientific SuperSignal ECL substrates are HRP substrates that offer excellent performance in western blotting applications, with longer light emission and stronger signal intensity than other luminol-based or acridan-based detection systems.
Learn More Western Blot Detection Reagents
With a variety of detection techniques for western blotting-chemiluminescence, fluorescence, or colorimetric, to choose from, you can select a technology to match your experimental requirements. Chemiluminescent Detection Technical Tip Fluorescent Detection Technical Tip, Protein Electrophoresis and...
Learn More Western Blot Detection Support
For Research Use Only. Not for use in diagnostic procedures., View all Can I use my own blocking reagent or antibody incubation solution with the iBind™ Western System? How does western detection with Alexa Fluor™ 680 and 790 secondary antibodies compare to chemiluminescent detection?
Learn More Fluorescent Western Blotting
Fluorescent western blotting is growing in popularity because it allows the ability to perform multiplex detection, where multiple proteins can be detected at the same time. Historically, the instrumentation available for fluorescent detection was not able to offer the sensitivity required by many...
Learn More Western Blot Protocols and Recipes
A western blot experiment, or western blotting, is a routine technique for protein analysis. The specificity of the antibody-antigen interaction enables a target protein to be identified in the midst of a complex protein mixture.