Documents & Support

Protocol: Protein electrophoresis and western blot recipes Product Literature

Brochure: High-throughput western blotting Product Literature

Brochure: Western Blotting Laboratory supply checklist Product Literature

User Guide: Spectra Multicolor Low Range Protein Ladder Manual / Product Insert

  • Version: B.0
Catalog # 26628

Journal: BioProbes 81 Journal Of Cell Biology Applications Product Literature

User Guide: PageRuler Broad Range Unstained Protein Ladder Manual / Product Insert

  • Version: B.0
Catalog # 26630

User Guide: PageRuler Unstained High Range Protein Ladder Manual / Product Insert

  • Version: B.0
Catalog # 26637

User Guide: PageRuler Low Range Unstained Protein Ladder Manual / Product Insert

  • Version: B.0
Catalog # 26632

Quick Reference: iBright CL750 Imaging System Manual / Product Insert

  • Version: B
Catalog #

Quick Reference: iBright CL1500 and FL1500 Imaging Systems Manual / Product Insert

  • Version: C
Catalog #

Application Note: Fluorescent western blotting - an introduction for new users (Chinese) Product Literature

What is the difference between Western Blot Signal Enhancer and SuperSignal Western Blot Enhancer? Is there any benefit to one versus the other? Product FAQ

Answer

The reagents in both kits are different. Western Blot Signal Enhancer (Cat. No. 21050) is a 2-step pre-treatment that occurs before blocking. It basically relaxes the protein on the membrane making the epitope easier to detect. SuperSignal Western Blot Enhancer (Cat. Nos. 46640, 46641) contains a membrane treatment reagent and a primary antibody diluent that increase both signal intensity and sensitivity 3- to 10-fold compared to a detecion performed without it. We have found that SuperSignal Western Blot Enhancer shows much less background while enhancing signal and sensitivity when compared to Western Blot Signal Enhancer.

Answer Id:: E15178

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Brochure: High-throughput western blotting (Chinese) Product Literature

User Guide: Pierce Fast Western Blot Kit ECL Substrate Manual / Product Insert

  • Version: Jan. 2015
Catalog #

Do I have to use a SuperSignal Western Blot substrate after using the SuperSignal Western Blot Enhancer? Product FAQ

Answer

Not necessarily. The SuperSignal Western Blot Enhancer will work with any chemiluminescent substrate (including SuperSignal Western Blot substrates), as well as colorimetric or fluorometric detection systems.

Answer Id:: E15179

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