Product Literature

Protein Standards Selection Guide

Product Literature

Protein transfer technical handbook

Manual / Product Insert

Pichia methanolica Expression Kit

Version: 25 October 2010
Catalog #
  • K178001(Discontinued)

Manual / Product Insert

Exosome CD63 (protocol for western blot)

Version: Rev 1.0 (2 June 2013)
Catalog #

Manual / Product Insert

E-PAGE MagicMark Unstained Protein Standard

Version: 17 January 2012
Catalog #
  • LC5701(Discontinued)

Product Literature

NuPAGE® Large Protein Kits and HiMark™ Protein Standards

Product FAQ

Can I combine MagicMark XP Western Protein standard with one of your prestained standards?

Answer

You can combine 5 µL MagicMark XP Standard with 5 µL Invitrogen Sharp Prestained Standard or 10 µL MagicMark XP Standard with 5 µL SeeBlue Prestained Standard in the same run using the same lane. The colored bands from the prestained standard can be used to confirm gel run and transfer. Following immunodetection, sharp MagicMark XP bands will develop directly on the western blot.

Answer Id: E11743

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Manual / Product Insert

User Guide: O-GlcNAc Western Blot Detection Kit

Version: Jan. 2015
Catalog #
  • 24565(Discontinued)

Manual / Product Insert

User Guide: Pierce Far-Western Biotinylated-Protein:Protein Interaction Kit

Version: Jan. 2015
Catalog #

Product FAQ

What are the molecular weights of the proteins in the MagicMark XP Western Protein Standard?

Answer

The MagicMark XP Western Protein Standard is designed for molecular weight estimation of proteins directly on western blots. Each protein in the standard contains an IgG binding site, allowing direct visualization with the same antibody-conjugate reagents used to detect the target protein on blots. Colorimetric, chemiluminescent, or fluorescent detection methods can be used for analysis. MagicMark XP Western Protein Standard is compatible with NuPAGE and Tris-Glycine gels. Here are the molecular weights of the proteins in the MagicMark XP Western Protein Standard:

- Band 1: 220 kDa
- Band 2: 120 kDa
- Band 3: 100 kDa
- Band 4: 80 kDa
- Band 5: 60 kDa
- Band 6: 50 kDa
- Band 7: 40 kDa
- Band 8: 30 kDa
- Band 9: 20 kDa

Answer Id: E11740

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Citations & References

MagicMark and SeeBlue Protein Standards can be run together in the same lane by following a few simple guidelines

  • Authors: Allen Bautista, Barbara Kempf, James Frazier
  • Journal: Focus (2002) 24:8-10
Catalog #

Manual / Product Insert

WesternDot 625 Western Blot Kits

Version: 07-02-2008

Product FAQ

What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation or Western blot analysis?

Answer

The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).

Answer Id: E4434

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