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Ion AmpliSeq™ Exome RDY Kit 1x8 (Ion Torrent™)

The Ion AmpliSeq Exome RDY Kit enables the fastest and simplest method of exome enrichment, now even easier with oligo pools for ultra-high multiplex PCR exome enrichment dried down in a 96-well plate. With half the number of target amplification pipetting steps compared to the original protocol and the elimination of small pipetting volumes, exome libraries can now be produced with unparalleled ease in under six hours with less than 50 minutes of hands-on time. Also, the Ion AmpliSeq Exome RDY Kit is compatible with the Ion Library Equalizer Kit for ease in library normalization. Leveraging the ultra-high multiplex PCR approach of Ion AmpliSeq technology with template preparation on the Ion Chef System and sequencing on an Ion Proton or Ion S5 System, the Ion AmpliSeq Exome RDY Kit allows for rapid sequencing of key exonic regions of the genome, going from DNA to variants in just two days.

The Ion AmpliSeq Exome RDY Kit 1x8 configuration includes the following components for eight exomes (two exomes per Ion PI v3 chip or Ion 540 Chip, and four exomes per Ion 550 Chip):

• Ion AmpliSeq Exome RDY panel: dried down oligo pools/primers in one 96-well plate with all eight rows filled (1x8), for ultra-high multiplex PCR enrichment of the exonic regions of the genome
• Ion AmpliSeq Library Kit Plus (8 exomes) for robust library preparation of Ion AmpliSeq Exome libraries using Ion AmpliSeq technology

The Ion AmpliSeq Exome RDY Kit enables you to:
• Simplify exome enrichment through the combination of dried-down primers and the Ion AmpliSeq workflow, with less than one hour of hands-on time and less than six hours total time
• Accelerate your disease research with rapid exome sequencing—DNA to variants in just two days
• Access affordable exome sequencing—no need to wait and batch dozens of samples or otherwise spend the high price of a lower-throughput run
• Maximize sequencing efficiency due to high performance specifications—designs covering >97% of the Consensus Coding Sequences (CCDS), >90% base on-target, and >90% coverage uniformity
• Identify annotated, filtered variants without bioinformatics expertise through fully integrated analysis with Torrent Suite and Ion Reporter software

Quickest exome enrichment with unprecedented simplicity
The Ion AmpliSeq Exome RDY Kit 1x8 configuration provides unmatched simplicity for exome enrichment. Primers have been dried down in a 96-well plate, using the 12 wells of a single row for each of the 12 primer pools, leading to the use of one row per exome. The Ion AmpliSeq Exome RDY S5 Kit 1x8 configuration contains a single 96-well plate, containing eight rows pre-filled with dried down primers, sufficient to prepare eight exome libraries. Additionally, the dried-down primers contain a light blue dye for easy identification of used or occupied rows. With a workflow requiring less than 50 minutes of hands-on time and no centrifugation steps, the kit enables exome library preparation in less than six hours. Additionally, the Ion AmpliSeq Exome RDY Kit maintains a low input requirement of 50 ng DNA. With the included Ion AmpliSeq Library Kit Plus, exome library preparation is more robust, resulting in higher uniformity and consistently higher library yield when performing library amplification. Also, the Ion AmpliSeq Exome RDY Kit is now compatible with the Ion Library Equalizer Kit for ultimate ease in library normalization.

Lowest cost per exome using a benchtop sequencer
The Ion AmpliSeq Exome RDY Kit allows disease researchers to access affordable exome sequencing on their benchtops without long turn-around times or the sample batching normally required to achieve a low cost per sample. Using the Ion AmpliSeq Exome RDY Kit with the Ion Proton or Ion S5 System enables you to go from DNA to variants in as little as two days. Additionally, with the most uniform exome enrichment covering >97% of the CCDS, you can achieve the most efficient sequencing with two exomes per Ion PI or Ion 540 Chip and four exomes per Ion 550 Chip.

Fully integrated analysis without bioinformatics expertise
Use of the kit with Torrent Suite and Ion Reporter software provides a simple and streamlined analysis workflow that automates the identification of your variants of interest without needing the expertise or infrastructure of a full bioinformatics team. Additionally, the Ion Proton or Ion S5 Sequencer along with Ion Reporter Software deliver both SNP and indel information along with copy number changes through a single analysis workflow, advancing your human disease research.

Ion AmpliSeq™ Exome RDY Kit 4x2 (Ion Torrent™)

The Ion AmpliSeq Exome RDY Kit enables the fastest and simplest method of exome enrichment, now even easier with oligo pools for ultra-high multiplex PCR exome enrichment dried down in a 96-well plate. With half the number of target amplification pipetting steps compared to the original protocol and the elimination of small pipetting volumes, exome libraries can now be produced with unparalleled ease in under six hours with less than 50 minutes of hands-on time. Also, the Ion AmpliSeq Exome RDY Kit is compatible with the Ion Library Equalizer Kit for ease in library normalization. Leveraging the ultra-high multiplex PCR approach of Ion AmpliSeq technology with template preparation on the Ion Chef System and sequencing on the Ion Proton or Ion GeneStudio S5 next-generation sequencers, the Ion AmpliSeq Exome RDY Kit allows for rapid sequencing of key exonic regions of the genome, going from DNA to variants in just two days.

The Ion AmpliSeq Exome RDY Kit 4x2 configuration includes the following components for eight exomes (two exomes per Ion PI v3 chip or Ion 540 Chip, and four exomes per Ion 550 Chip):

• Ion AmpliSeq Exome RDY panel: dried down oligo pools/primers in four 96-well plates, each with two rows (C and F) filled (4x2), for ultra-high multiplex PCR enrichment of the exonic regions of the genome
• Ion AmpliSeq Library Kit Plus (8 exomes) for robust library preparation of Ion AmpliSeq Exome libraries using Ion AmpliSeq technology

The Ion AmpliSeq Exome RDY Kit enables you to:
• Simplify exome enrichment through the combination of dried-down primers and the Ion AmpliSeq workflow, with less than one hour of hands-on time and less than six hours total time
• Accelerate your disease research with rapid exome sequencing—DNA to variants in just two days
• Access affordable exome sequencing—no need to wait and batch dozens of samples or otherwise spend the high price of a lower-throughput run
• Maximize sequencing efficiency due to high performance specifications—designs covering >97% of the Consensus Coding Sequences (CCDS), >90% base on-target, and >90% coverage uniformity
• Identify annotated, filtered variants without bioinformatics expertise through fully integrated analysis with Torrent Suite and Ion Reporter software

Quickest exome enrichment with unprecedented simplicity
The Ion AmpliSeq Exome RDY Kit 4x2 configuration provides unmatched simplicity for exome enrichment. Primers have been dried down into 96-well plates, using the 12 wells of a single row for each of the 12 primer pools, leading to the use of one row per exome. The Ion AmpliSeq Exome RDY S5 Kit 4x2 configuration contains four 96-well plates, each with two rows pre-filled with dried-down primers, sufficient to prepare eight exome libraries. Additionally, the dried-down primers contain a light blue dye for easy identification of used or occupied rows. With a workflow requiring less than 50 minutes of hands-on time and no centrifugation steps, the kit enables exome library preparation in less than six hours. Additionally, the Ion AmpliSeq Exome RDY Kit maintains a low input requirement of 50 ng DNA. With the included Ion AmpliSeq Library Kit Plus, exome library preparation is more robust, resulting in higher uniformity and consistently higher library yield when performing library amplification. Also, the Ion AmpliSeq Exome RDY Kit is now compatible with the Ion Library Equalizer Kit for ultimate ease in library normalization.

Lowest cost per exome using a benchtop sequencer
The Ion AmpliSeq Exome RDY Kit allows disease researchers to access affordable exome sequencing on their benchtops without long turn-around times or the sample batching normally required to achieve a low cost per sample. Using the Ion AmpliSeq Exome RDY Kit with the Ion Proton or Ion S5 System enables you to go from DNA to variants in as little as two days. Additionally, with the most uniform exome enrichment covering >97% of the CCDS, you can achieve the most efficient sequencing with two exomes per Ion PI or Ion 540 Chip and four exomes per Ion 550 Chip.

Fully integrated analysis without bioinformatics expertise
Use of the kit with Torrent Suite and Ion Reporter software provides a simple and streamlined analysis workflow that automates the identification of your variants of interest without needing the expertise or infrastructure of a full bioinformatics team. Additionally, the Ion Proton or Ion S5 Sequencer along with Ion Reporter Software deliver both SNP and indel information along with copy number changes through a single analysis workflow, advancing your human disease research.

Ion PGM™ Controls Kit v3 (Ion Torrent™)

The Ion PGM™ Controls Kit v3 contains control genomic DNA and control libraries for 200 and 400 bp run configurations using the Ion PGM semiconductor-based sequencing platform. The kit provides for greater experimental control through the use of quality control reagents for the semiconductor-based sequencing workflow and allows you to assess sample quality, save time and reagents, and maximize productivity.

The Ion PGM Controls Kit v3 provides you with:

• Quality control tools for greater experimental control
• Significant time and cost savings through identification of suboptimal samples
• Support for 200 and 400 bp run configurations

Ion PGM™ Hi-Q™ View OT2 Kit (Ion Torrent™)

The Ion PGM™ Hi-Q™ View OT2 Kit enables accurate and reproducible template preparation for libraries up to 400 base pairs using the Ion OneTouch™ 2 System and is compatible with the Ion PGM™ System. As an integral component of the semiconductor sequencing workflow, the Ion PGM Hi-Q View OT2 Kit is designed to produce high-quality Ion Sphere™ particles and is designed for use in combination with the Ion PGM™ Hi-Q™ View Sequencing Kit.

• Delivers template preparation for 200 and 400 bp libraries
• Minimizes user variability with new visible Ion Sphere particles
• Offers templating controls to help ensure high quality sequencing results
• Enables increased lab throughput with multiplexing support

Maximize your results with high accuracy templating chemistry
The Ion PGM Hi-Q View OT2 Kit is an integral component of the Ion PGM™ System sequencing workflow. Designed for users of the Ion OneTouch 2 system, the Ion PGM Hi-Q View OT2 Kit is optimized for users looking to maximize their sequencing data quality. The Ion PGM Hi-Q View OT2 Kit contains the most advanced templating chemistry available to Ion PGM customers. Use the Ion PGM Hi-Q View OT2 Kit to maximize your variant calling performance and improve de novo sequencing research applications.

A single kit to meet your Ion PGM sequencing needs
The Ion PGM Hi-Q View OT2 Kit is configured to support your sequencing needs for applications requiring 200 or 400 bp read lengths. When combined with the Ion PGM Hi-Q View Sequencing Kit, the Ion PGM Hi-Q View OT2 Kit offers optimal performance. This kit supports the Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, Ion 318™ Chip v2 BC. The kit includes sufficient reagents for eight templating reactions. The kit components include Ion Sphere particles, buffers, reagents, and supplies.

Ion PGM™ Hi-Q™ View Chef Kit (Ion Torrent™)

The Ion PGM™ Hi-Q™ View Chef Kit enables accurate and reproducible template preparation, chip loading, and sequencing of 200 bp libraries on the Ion Chef™ System and the Ion PGM™ System. The plug-and-play, pre-packaged, single-use reagent cartridges enable a fully automated workflow, processing two samples per run for increased productivity. Reagent and sample barcoding features help to verify the user-defined run plan and offer complete run traceability.

• Delivers automated template preparation and chip loading for 200 bp libraries
• Provides the most accurate sequencing chemistry available for the Ion PGM System
• Designed to minimize the hands-on time required to prepare your samples
• Helps increase lab throughput with dual sample processing

Maximize your results with high-accuracy sequencing chemistry
The Ion PGM Hi-Q View Chef Kit is an integral component of the Ion Torrent™ next-generation sequencing workflow. Designed to simplify the overall workflow with minimal hands-on time, the Ion Chef System is ideal for users of all experience levels. The Ion PGM Hi-Q View Chef Kit contains the most advanced templating and sequencing chemistries available to Ion PGM customers. Use the Ion PGM Hi-Q View Sequencing Kit to help maximize your variant calling performance and improve de novo sequencing applications.

A single kit to meet your Ion PGM sequencing needs
The Ion PGM Hi-Q View Chef Kit is configured to support all of your sequencing needs for applications requiring 200 bp read lengths. This kit supports the Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, and the Ion 318™ Chip v2 BC. The kit includes sufficient reagents for eight 200 bp sequencing reactions.

For 400-base applications, order the Ion PGM™ Hi-Q™ View Chef 400 Kit (Cat. No. A30798), which provides all the reagents needed for eight 400 bp sequencing reactions.

Ion PGM™ Hi-Q™ View Sequencing Kit (Ion Torrent™)

The Ion PGM™ Hi-Q™ View Sequencing Kit contains reagents and consumables for robust and highly accurate sequencing of 200 and 400 base pair libraries using the Ion OneTouch™ 2 System combined with the Ion PGM™ System. This kit offers the most advanced sequencing chemistry available to users of the Ion PGM System, and its economical price brings next-generation sequencing capability to every lab. Use the Ion PGM Hi-Q View Sequencing Kit to maximize your variant calling performance.

Note: an Ion PGM™ Wash 2 Bottle Kit is required for use of this sequencing kit. One Wash 2 Bottle kit may be used for up to 40 sequencing runs before replacement.

• Delivers the latest advancements to data quality and accuracy using the Ion PGM System
• Provides a single unified solution for both 200 and 400 bp sequencing
• Offers low cost per sample through the use of multiplexing

Maximize your results with high accuracy sequencing chemistry
The Ion PGM Hi-Q View Sequencing Kit is configured to support all of your sequencing needs for applications requiring 200 bp or 400 bp read lengths. This kit supports the Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, and Ion 318™ Chip v2 BC and is compatible with the Ion PGM™ Hi-Q™ View OT2 Kit. The kit components include sequencing solutions, sequencing reagents, and supplies.

Ion PGM™ Hi-Q™ View Chef 400 Kit (Ion Torrent™)

The Ion PGM™ Hi-Q™ View Chef 400 Kit enables accurate and reproducible template preparation, chip loading, and sequencing of 400 bp libraries on the Ion Chef™ System and is compatible with the Ion PGM™ System. The plug-and-play, pre-packaged, single-use reagent cartridges enable a fully automated workflow, processing two samples per run for increased productivity. Reagent and sample barcoding features help to verify the user-defined run plan and offer complete run traceability.

• Delivers automated template preparation and chip loading for 400 bp libraries
• Provides the most accurate sequencing chemistry available for the Ion PGM System
• Designed to minimize the hands-on time required to prepare your samples
• Helps increase lab throughput with dual sample processing

Maximize your results with high accuracy sequencing chemistry
The Ion PGM Hi-Q View Chef 400 Kit is an integral component of the Ion Torrent™ next-generation sequencing workflow. Designed to simplify the overall workflow with minimal hands-on time, the Ion Chef System is ideal for users of all experience levels. The Ion PGM Hi-Q View Chef 400 Kit contains the most advanced templating and sequencing chemistries available to Ion PGM customers. Use the Ion PGM Hi-Q View Sequencing Kit to help maximize your variant calling performance and improve de novo sequencing applications.

A single kit to meet your Ion PGM sequencing needs
The Ion PGM Hi-Q View Chef Kit is configured to support all of your sequencing needs for applications requiring 400 bp read lengths. This kit supports the Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, and the Ion 318™ Chip v2 BC. The kit includes sufficient reagents for eight 400 bp sequencing reactions.

For 200-base applications, order the Ion PGM™ Hi-Q™ View Chef Kit (Cat. No. A29902), which provides all the reagents needed for eight 200 bp sequencing reactions.