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BupH™ Carbonate-Bicarbonate Buffer Packs (Thermo Scientific™)

Thermo Scientific BupH Carbonate-Bicarbonate Buffer Packs are pouches of dry-blended powder that are each sufficient to make 500 mL of alkaline (pH 9.4) Na-carbonate (carb-bicarb) buffer for use in protein and antibody coating procedures.

BupH Dry-Blend Packs of carbonate-bicarbonate buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 0.2M carbonate-bicarbonate, pH 9.4.

Features of BupH Carbonate-Bicarbonate Buffer Packs:

Carb-bicarb buffer—dissolved in 500 mL of water, each pack makes 0.2M carbonate-bicarbonate, pH 9.4
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

Applications:
• Coating or binding capture-antibodies and other proteins onto polystyrene microplates for ELISA and other plate assays

BupH™ Borate Buffer Packs (Thermo Scientific™)

Thermo Scientific BupH Borate Buffer Packs are pouches of dry-blended powder that are each sufficient to make 500 mL of borate buffer for use in protein modification procedures requiring amine-free conditions and alkaline pH.

Features of BupH Borate Buffer Packs:

Borate buffer—dissolved in 500 mL of water, each pack makes 50 mM borate, pH 8.5
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

BupH Dry-Blend Packs of borate buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 50 mM borate, pH 8.5.

Applications:
• Protein modification procedures requiring amine-free buffer at alkaline pH

Related Products
Pierce™ 20X Borate Buffer

BupH™ MES Buffered Saline Packs (Thermo Scientific™)

Thermo Scientific BupH MES Buffered Saline Packs are pouches of dry-blended powder that are each sufficient to make 500 mL of optimized amine-free and carboxyl-free buffer for carbodiimide (EDC) crosslinking and conjugation reactions.

Features of BupH MES Buffered Saline Packs:

MES buffer—dissolved in 500 mL of water, each pack makes 0.1M MES, 0.9% sodium chloride, pH 4.7
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

BupH Dry-Blend Packs of MES buffered saline are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 0.1M MES, 0.9% sodium chloride, pH 4.7. MES is 2-(N-morpholino) ethanesulfonic acid.

Applications:
• Carboxyl-to-amine crosslinking procedures using carbodiimide crosslinker EDC (Part No. 22980)

BupH™ Tris-HEPES-SDS Running Buffer (Thermo Scientific™)

Thermo Scientific BupH Tris-HEPES-SDS Buffer Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of running buffer for gel electrophoresis (SDS-PAGE) with Thermo Scientific Precise Precast Protein Gels.

Features of Thermo Scientific BupH Tris-HEPES-SDS Buffer Packs:

Tris-HEPES-SDS buffer—dissolved in 500 mL of water, each pack makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

Applications:
• Running buffer for SDS-PAGE with Thermo Scientific Precise Precast Gels (e.g., Part No. 25224)

BupH Dry-Blend Packs of Tris-HEPES-SDS buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8. HEPES is 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid.

Related Products
Pierce™ 20X Tris-HEPES-SDS Buffer

BupH™ Tris-Glycine Buffer Packs (Thermo Scientific™)

Thermo Scientific BupH Tris-Glycine Transfer Buffer Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of standard transfer buffer for wet or semi-dry electrophoretic protein transfer from gel to blotting membrane.

Features of Thermo Scientific BupH Tris-Glycine Transfer Buffer Packs:

Tris-glycine buffer—dissolved in 400 mL of water and 100 mL methanol, makes 25 mM Tris, 192 mM glycine, pH 8
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

Applications:
• Running buffer for gel-to-membrane electrophoretic transfer

BupH Dry-Blend Packs of Tris-glycine buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 400 mL of water and 100 mL methanol, makes 25 mM Tris, 192 mM glycine, pH 8.

Related Products
Pierce™ 10X Tris-Glycine Buffer

BupH™ Tris Buffered Saline Packs (Thermo Scientific™)

Thermo Scientific BupH Tris Buffered Saline Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of Tris-NaCl buffer (TBS) for diluents and wash buffers in ELISA, Western blotting and general immunoassay procedures.

Features of Thermo Scientific BupH Tris Buffered Saline Packs:

TBS—dissolved in 500 mL of water, each pack makes 25 mM Tris, 0.15M NaCl, pH 7.2
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

Applications:
• Wash buffer and antibody diluent for ELISA, Western blotting and other immunoassays

BupH Dry-Blend Packs of TBS are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 25 mM Tris, 0.15M NaCl, pH 7.2.

Related Products
Pierce™ 20X TBS Buffer

BupH™ Tris-Glycine-SDS Buffer Packs (Thermo Scientific™)

Thermo Scientific BupH Tris-Glycine-SDS Buffer Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of standard (pH 8.3) running buffer for SDS-PAGE (protein polyacrylamide gel electrophoresis).

Features of Thermo Scientific BupH Tris-Glycine-SDS Buffer Packs:

Tris-glycine-SDS buffer—dissolved in 500 mL of water, each pack makes 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

Applications:
• Running buffer for Tris-glycine gel electrophoresis

BupH Dry-Blend Packs of Tris-glycine-SDS buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3.

Related Products
Pierce™ 10X Tris-Glycine SDS Buffer

BupH™ Modified Dulbecco's PBS Packs (Thermo Scientific™)

Thermo Scientific BupH Modified Dulbecco's PBS Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of physiological Na- and K-phosphate buffered saline (D-PBS) for ELISA and Western blot diluents and wash buffers.

Features of BupH Modified Dulbecco's PBS Packs:

Dulbecco's PBS—dissolved in 500 mL of water, each pack makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, pH 7.4
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

BupH Dry-Blend Packs of modified Dulbecco's phosphate buffered saline are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, pH 7.4.

Applications:
• Wash buffers and antibody diluents in applications such as ELISA, Western blotting and other immunoassays

Related Products
Pierce™ 20X Modified Dulbecco's PBS Buffer

BupH™ Phosphate Buffered Saline Packs (Thermo Scientific™)

Thermo Scientific BupH Phosphate Buffered Saline Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of sodium phosphate buffer (PBS) for use in crosslinking and labeling reactions requiring amine-free conditions.

Features of BupH Phosphate Buffered Saline Packs:

PBS—dissolved in 500 mL of water, each pack makes 0.1M sodium phosphate, 0.15M NaCl, pH 7.2
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

BupH Dry-Blend Packs of PBS are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 0.1M sodium phosphate, 0.15M NaCl, pH 7.2.

Applications:
• Crosslinking, biotinylation and fluorescent labeling reactions requiring an amine-free buffer

Related Products
Pierce™ 20X Phosphate Buffered Saline