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User Guide: SuperScript IV CellsDirect cDNA Synthesis Kit Manual / Product Insert

  • Version: A.0
Catalog #

Product Insert: TaqMan Advanced miRNA cDNA Synthesis Kit Manual / Product Insert

  • Version: A.0
Catalog # A28007

Which qPCR reagents do you recommend using with the SuperScript IV CellsDirect cDNA Synthesis Kit? Product FAQ

Answer

For qPCR analysis with the SuperScript IV CellsDirect cDNA Synthesis Kit, we recommend using the PowerTrack SYBR Green Master Mix (Cat. No. A46012) for SYBR Green assays and the TaqMan Fast Advanced Master Mix (Cat. No. 4444556) for TaqMan assays.

Answer Id:: E18265

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Can I use the SuperScript IV CellsDirect cDNA Synthesis Kit for RNA detection from adherent cells? Product FAQ

Answer

Yes, the SuperScript IV CellsDirect cDNA Synthesis Kit can be used with different types of cultured cells: adherent, suspension, and FACS-sorted cells.

Answer Id:: E18266

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Package Inserts: HT One-Cycle cDNA Synthesis Kit Manual / Product Insert

  • Version: JAN 2017

User Guide: DyNAmo cDNA Synthesis Kit Manual / Product Insert

  • Version: Jan. 2015
Catalog # F470L(Discontinued)

User Guide: Verso cDNA Synthesis Kit, AB1453B Manual / Product Insert

  • Version: Jul 2015, Rev. 8
Catalog # AB1453B

User Guide: Maxima First Strand cDNA Synthesis Kit RT-qPCR w dsDNase, K1671 Manual / Product Insert

  • Version: Jan. 2015
Catalog # K1671

Package Inserts: WT Amplified Double-Stranded cDNA Synthesis Kit Manual / Product Insert

  • Version: JAN 2017

Which cell lines have been tested to work with the SuperScript IV CellsDirect cDNA Synthesis Kit? Product FAQ

Answer

Here is a list of cell lines that have been tested to work with the SuperScript IV CellsDirect cDNA Synthesis Kit:

Cell line; Culture properties; Organism; Tissue
- HeLa; adherent; Homo sapiens; cervical adenocarcinoma
- HeLa S3; suspension; Homo sapiens; cervical adenocarcinoma
- Raji; suspension; Homo sapiens; B lymphocyte
- NIH/3T3; adherent; Mus Musculus (mouse); embryonic fibroblast
- HEK-293; adherent; Homo sapiens; kidney
- Jurkat; suspension; Homo sapiens; acute T cell leukemia
- Daudi; suspension; Homo sapiens; Burkitt's lymphoma
- K562; suspension; Homo sapiens; bone marrow
- iPSC; adherent; Homo sapiens; stem cells
- Balb/3T3; adherent; Mus Musculus (mouse); embryonic fibroblast

Answer Id:: E18267

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User Guide: 200 rxns, Maxima First Strand cDNA Synthesis Kit for RT-qPCR Manual / Product Insert

  • Version: Jan. 2015
Catalog # K1642

Package Inserts: Two-Cycle cDNA Synthesis Kit Manual / Product Insert

  • Version: JAN 2017

If I run out of Lysis Solution in the SuperScript IV CellsDirect cDNA Synthesis Kit, can I use my home-brewed lysis solution or lysis solution from another kit? Product FAQ

Answer

All the reagents in the SuperScript IV CellsDirect cDNA Synthesis Kit have been tested and optimized for the direct RT protocol. It's important to use only reagents offered in the kit and follow the protocol. Efficiency could be compromised if lysis solution from other sources is used.

Answer Id:: E18285

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User Guide: Maxima First Strand cDNA Synthesis Kit RT-qPCR w dsDNase, K1672 Manual / Product Insert

  • Version: Jan. 2015
Catalog # K1672

When using the SuperScript IV CellsDirect cDNA Synthesis Kit, how should I remove gDNA from the final reaction? Product FAQ

Answer

For removing gDNA from the final reaction we recommend the following:
- Ensure DNase I is added to the Lysis Solution and the incubation is performed as described.
- Ensure the lysis reagents are kept on ice throughout the procedure.
- Ensure the lysis reaction is performed at room temperature.
- Use exon‑exon junction primers that are cDNA‑specific and do not bind to genomic DNA.
- Make sure that all media is removed from the wells/tube (cells).

Answer Id:: E18268

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