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CD274 (PDL1) Recombinant Human Protein, hIgG1-Fc tag

CD274 molecule (CD274), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the N-terminal segment (Met 1-Thr 239) of the extracellular domain of human CD274 (NP_054862.1) fused to the Fc region of human IgG1 at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA
. Immobilized human PDCD1/PD1 at 10 µg/ml (100 µg/well) can bind recombinant human CD274/PDL1-Fc chimera with a linear range of 0.02-0.4 µg/ml
.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

GPC3 Recombinant Human Protein, His Tag

Glypican 3 (GPC3), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the human GPC3 (P51654-1) (Met 1-His 559) fused to a C-terminal polyhistidine tag.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human GPC3 at 5 µg/ml (100 µl/well) can bind human bFGF / FGF2 with a linear ranger of 0.156-2.5 ng/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

CD4 Recombinant Human Protein, hIgG1-Fc.His Tag

Manufactured by Sino Biological (China); Distributed by Life Technologies.

Cluster of Differentiation 4 (CD4) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in the study of protein-protein interactions, and cell biology research applications. It can also be used as a protein standard.

This recombinant human protein is expressed from a DNA sequence encoding the extracellular domain (Met 1-Trp 390) of human CD4 (NP_000607.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

N-terminal Sequence Analysis: Lys26.

Activity: measured by its binding ability in a functional ELISA. Immobilized human CD4-His at 2 µg/well has been shown to bind biotinylated HIV-1 GP120 with a linear range of 0.078-1.25 µg/mL.

Formulation: lyophilized in PBS, pH 7.4, 5% mannitol, 5% trehalose, and 0.02% Tween®-80.

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: expires one year from date of receipt when stored as instructed.

Background
Human T-cell surface glycoprotein CD4, also known as T-cell surface antigen T4/Leu-3, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like Vtype (immunoglobulin-like) domain. CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 amplifies the signal generated by the TCR. CD4 interacts directly with MHC class II molecules on the surface of the antigen presenting cell via its extracellular domain. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.

You may also be interested in the following CD4 products:
Primary Antibodies
Gene Expression Assays

CD96 Recombinant Mouse Protein, His Tag

CD96 antigen (CD96), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of mouse CD96 (Q3U0X8) (Met 1-Met 536) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD96 at 10 µg/ml (100 µl/well) can bind mouse PVR with a linear range of 6.4-160 ng/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

PDGFRB Recombinant Human Protein (without Catalytic Activity Domain), His Tag

Platelet-derived growth factor receptor, beta polypeptide (PDGFRB), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies..

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Lys 531) of human PDGFRB (NP_002600.1) fused to a polyhistidine tag at the C-terminus.

Activity: This recombinant protein has not been tested.

Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by SINO Biological.

CD97 Recombinant Human Protein, hIgG1-Fc Tag

CD97 molecule, recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gln 398) of human CD97 isoform 2 (NP_001775.2) fused to the Fc region of human IgG1 at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA . Immobilized human CD55 at 2 µg/ml(100 µl/well) can bind human CD97 with a linear range of 1.28-32 ng/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

CD4 Recombinant Human Protein, His Tag

Cluster of Differentiation 4 (CD4), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Trp 390) of human CD4 (NP_000607.1) fused to a polyhistidine tag at the C-terminus.

Activity:
1. Measured by its ability to bind towith HIV-1 gp120 in a functional ELISA.< BR> 2. Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells . When 5 x 104 cells/well are added to CD4 coated plates (2.5 µg/mland 100 µl/well), approximately 60-80% will adhere specifically after 60 minutes at 37°C.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

CD63 Recombinant Human Protein, His Tag

Manufactured by Sino Biological (China); Distributed by Life Technologies.

Cluster of Differentiation 63 (CD63) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant human protein is expressed from a DNA sequence encoding the second the extracellular domain (Ala 103-Val 203) of human CD63 (P08962-1) fused to both a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

Activity: this recombinant protein has not been tested.

Formulation: lyophilized in PBS, pH 7.4, 5% mannitol, 5% trehalose, and 0.02% Tween®-80.

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: expires one year from date of receipt when stored as instructed.

Background
CD63, is a multi-pass membrane protein which belongs to the tetraspanin (TM4SF) family. Tetraspanins participate in a variety of cellular processes, likecell activation, adhesion, differentiation and tumour invasion. CD63 / Tspan-30 is expressed in melanoma and appears to be involved in regulation of motility and adhesion of melanoma cells. CD63 / Tspan-30 is associated with early stages of melanoma tumor progression. It may play a role in growth regulation. Lack of expression of CD63 in platelets has been observed in a patient with Hermansky-Pudlak syndrome (HPS). which is a genetically heterogeneous, rare, autosomal recessive disorder characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects.

You may also be interested in the following CD63 products:
Organell Isolation
Primary Antibodies
Gene Expression Assays

FCGR3A (CD16a), 176 Phe/Val Recombinant Human Protein, His Tag

Fc gamma receptor III-a (FCGR3A/CD16a), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human FCGR3A (P08637-1), containing the mutation 176 Phe/Val, precursor fused to a polyhistidine tag at the C-terminus.

Activity: Measured using the Octet RED System, the affinity constant (Kd) of FCGR3A bound to human IgG1 was 180nM.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

SLAMF1 (CD150) Recombinant Human Protein, His Tag

Signaling lymphocytic activation molecule (SLAMF1/CD150), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the human SLAMF1 precursor (NP_003028.1), residues 1 to 258, fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human SLAMF1-His at 1 µg/well can bind biotinylated recombinant human SH2D1A with a linear range of 0.8-20.0 µg/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

CD38 Recombinant Human Protein, His tag

ADP-Ribosyl Cyclase (CD38), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human CD38 (NP_001766.2) (Val 43-Ile 300) fused to a C-terminal polyhistidine tag was expressed.

Activity: The activity of this protein has not been tested.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

This protein is manufactured by Sino Biological Inc.

FCGR3A (CD16a), 158 Phe/Val Recombinant Human Protein, His.AVI Tag, Biotinylated

Fc gamma receptor III-a (FCGR3A/CD16a), recombinant human protein is supplied as a frozen format. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human FCGR3A (P08637-1), containing the 176 Phe/Val mutation, fused to a polyhistidine and AVI tags at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Activity: Measured by its ability to bind recombinant human IgG1 in a functional ELISA.

Formulation: PBS, pH 7.4, 5% Mannitol, 5% Trehalose, 10% glycerol, and 0.02% Tween 80.

Reconstitution: No reconstitution is needed. Store aliquots under sterile conditions at -80°C. Thaw protein on ice before use and avoid repeated freeze-thaw cycles.

This protein is manufactured by Sino Biological Inc.

PVR (CD155) Recombinant Human Protein, hIgG1-Fc Tag

Poliovirus receptor (PVR/CD155), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Asn 343) of human PVR (NP_006496.3) fused to the Fc region of human IgG1 at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA . Immobilized human DNAM1 at 2 µg/ml(100 µl/well) can bind human PVR-Fc with a linear range of 0.032-0.8 µg/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

CD200 Recombinant Mouse Protein, His tag

Cluster of Differentiation 200 (CD200), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 232) of mouse CD200 (NP_001076017.1) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA . Immobilized recombinant mouse CD200 at 1 µg/ml(100 µl/well) can bind mouse CD200R1-Fc with a linear range of 1.5-200 ng/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

BSG (CD147) Recombinant Mouse Protein, His tag

Basigin (BSG/CD147), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Arg 209) of mouse BSG isoform 2 (NP_001070652.1) fused to a polyhistidine tag at the C-terminus.

Activity: This recombinant protein has not been tested.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.