Documents & Support

1-15 of 3,139 Results

Regulatory statements vary by product. Please see product-specific literature to determine product use.

Do the 2-step Cells-to-CT kits contain RNase inhibitor? Product FAQ

Answer

Yes, the Stop Solution provided in the 2-step Cells-to-CT kits contains RNase inhibitor.

Answer Id: E20253

Was this answer helpful?

Yes No

Thank you for your response

Is 8% CO2 a growth requirement for CTS Viral Production Cells? Product FAQ

Answer

We generally recommend culturing the CTS Viral Production Cells under 8% CO2 conditions. We found that cells cultured in 5% CO2 grew slower and were more prone to more cell clumping than when grown in 8% CO2.

Answer Id: E17923

Was this answer helpful?

Yes No

Thank you for your response

What primers are used in the reverse transcription step for the Cells-to-CT kits? Product FAQ

Answer

The following 2-step Cells-to-CT kits use a mixture of oligo dT and random primers for the reverse transcription step:
• TaqMan Fast Advanced Cells-to-CT Kit
• TaqMan Gene Expression Cells-to-CT Kit
• SYBR Green Fast Advanced Cells-to-CT Kit
• Power SYBR Green Cells-to-CT Kit
• Fast SYBR Green Cells-to-CT Kit

The following 1-step Cells-to CT kits require gene-specific primers for the reverse transcription step, so you can either use your SYBR qPCR primers or TaqMan assay primers:
• Cells-to-CT 1-Step TaqMan Kit
• Cells-to-CT 1-Step Power SYBR Green Kit

Answer Id: E20013

Was this answer helpful?

Yes No

Thank you for your response

What are the shipping and storage conditions for the CTS Detachable Dynabeads CD3/CD28 Kit (Cat. No. A56992 and A56993)? Product FAQ

Answer

The CTS Detachable Dynabeads CD3/CD28 Kit (Cat. No. A56992 and A56993) is shipped at 2-8 degrees C and we strongly recommend storing it at 2-8 degrees C, protected from light. This is applicable to both the CTS Detachable Dynabeads provided in the vials, and the Release Buffer provided in a bioprocess container.

Find additional tips, troubleshooting help, and resources within our Dynabeads Cell Isolation and Expansion Support Center.

Answer Id: E22075

Was this answer helpful?

Yes No

Thank you for your response

Which cell lines have been tested to work with the Cells-to-CT system? Product FAQ

Answer

Here is a short list of cell lines that have been validated with the Cells-to-CT system: HeLa, HepG2, primary hepatocytes, SK-N-AS, SK-N-SH, U-87 MG, ME-180, A549, Jurkat, PC-12, PT-K75, NIH/3T3, Raji, HEK-293, COS-7, CHO-K1, NCI-H460, DU-145, K562, U-2 OS, Huh-7, Neuro 2A, and BJ. For additional information please visit the following page: https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/rna-extraction/rna-types/total-rna-extraction/cells-to-ct-kits/cells-to-ct-faq.html

Answer Id: E16570

Was this answer helpful?

Yes No

Thank you for your response

The manual recommends passaging the CTS Viral Production Cells three times before freezing. Can I bank the cells sooner than that? Product FAQ

Answer

For optimal cell recovery from liquid N2 storage, CTS Viral Production Cells should be passaged to passage 2 (P2) (from the original vial) and up to P3 before banking, to ensure good cell health and viability. Cells that demonstrate good cell health (i.e., ˜26 hr doubling time, round cell morphology, and minimal clumping) may be banked at P2 or P3.

Answer Id: E17920

Was this answer helpful?

Yes No

Thank you for your response

My CTS Viral Production Cells have low viability at 3 days post-thawing. Is this normal? Product FAQ

Answer

It is okay to see variation in viability upon thawing, but this should normalize during subsequent passages. We recommend culturing the cells as described in chapter 2 of the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018450_CTS_LV-MAX_LentiviralProdSys_UG.pdf) so that the cells recover sufficiently and achieve >90% viability before starting the protocol for CTS lentviral production.

Answer Id: E17901

Was this answer helpful?

Yes No

Thank you for your response

Which cell lines have been tested with the Cells-to-CT system? Product FAQ

Answer

This is a short list of the cell lines compatible with the Cells-to-CT system: HeLa, HepG2, Primary Hepatocytes, SK-N-AS, SK-N-SH, U-87 MG, ME-180, A549, Jurkat, PC-12, PT-K75, NIH/3T3, Raji, HEK-293, COS-7, CHO-K1, NCI-H460, DU-145, K561, U-2 OS, Huh-7, Neuro 2A, BJ.

Answer Id: E7103

Was this answer helpful?

Yes No

Thank you for your response

How many transductions can I perform with one CTS CytoTune-iPS 2.1 Sendai Reprogramming Kit? Product FAQ

Answer

The CTS CytoTune-iPS 2.1 Sendai Reprogramming Kit is sufficient to reprogram a minimum of six wells in a six-well plate. One well has a surface area of approximately 10 cm2.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E16079

Was this answer helpful?

Yes No

Thank you for your response

Do I need to order additional components with the CTS OpTmizer T Cell Expansion SFM (Cat. Nos. A1048501, A1048502, A1048503)? Product FAQ

Answer

The CTS OpTmizer T Cell Expansion SFM (Cat. Nos. A1048501, A1048502, A1048503) kit comes with OpTmizer T Cell Expansion Basal Medium and OpTmizer T Cell Expansion Supplement, which are mixed together prior to use. Depending on your protocol you may need to purchase T cell growth factors such as IL-2 (Cat. No. PHC0023) and L-glutamine (Cat. No. 25030) to form a complete medium. To boost growth of human T cells, human AB serum can be added. If desired, antibiotics such as Gentamicin (Cat. No. 15750), can also be added.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E21690

Was this answer helpful?

Yes No

Thank you for your response

My DAPI labeled samples have a strong blue background signal immediately after mounting. What can I do to fix this? Product FAQ

Answer

Some mounting medias can have strong blue autofluorescence. If you are seeing a high blue background, it could be coming from the mountant. Try labeling the sample and view it before (using a wet mount in buffer) and after mounting to determine if the background signal is coming from the mounting media or the sample itself.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E6484

Was this answer helpful?

Yes No

Thank you for your response

I am using a Cells-to-CT kit and I see signal from the genomic DNA in my real-time PCR results. How do I get rid of the genomic DNA contamination? Product FAQ

Answer

To prevent signal from genomic DNA in the Cells-to-CT real-time PCR reaction, we recommend using a TaqMan assay or primer set that spans an exon-exon boundary, and adding DNase I to degrade genomic DNA during the lysis reaction. For optimal DNase activity in the lysis reaction, we recommend the following:
1. Ensure all media is removed from the cells.
2. Wash each well or cell pellet with an equal volume of room temperature 1X PBS.
3. Ensure the lysis reaction happens at room temperature. The lysis reaction may not reach room temperature if the plate is on ice prior to adding Lysis Solution, or cold Lysis Solution is added.
4. Warm the Lysis Solution to room temperature before adding to the cells.
5. Perform the lysis reaction at 25 degrees C for up to 8 minutes.

Answer Id: E20251

Was this answer helpful?

Yes No

Thank you for your response

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained? Product FAQ

Answer

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E6424

Was this answer helpful?

Yes No

Thank you for your response

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish. Product FAQ

Answer

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E6439

Was this answer helpful?

Yes No

Thank you for your response

Can I transform 2 plasmids into the same cell? Product FAQ

Answer

Yes, this is possible. We recommend using saturating amounts of DNA (10 ng of each plasmid). Make sure that the origin of replication is different in each plasmid so that they can both be maintained in the cell. If the ori is the same, the plasmids will compete for replication and the one with even a slight disadvantage will be lost. Alternatively, cells with a resident plasmid can be electroporated with a second plasmid without “electrocuring” taking place.

Answer Id: E6712

Was this answer helpful?

Yes No

Thank you for your response

Results per page