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SnakeSkin™ Dialysis Tubing, 3.5K MWCO, 35 mm (Thermo Scientific™)

Thermo Scientific SnakeSkin Dialysis Tubing, 3.5K MWCO, is an easy and ready-to-use form of traditional dialysis membrane tubing that allows desalting and buffer exchange for 10 to 100 mL samples.

Features of SnakeSkin Dialysis Tubing, 3.5K MWCO, 35mm:

Convenient—ready to use, pre-wetted, pleated tube
High recovery—greater than 90% protein recovery
Speed—dialysis is generally completed in 4 to 6 hours
Stability—compatible with a variety of laboratory solutions, including acids, bases, hydrophobic solvents and alcohols

3.5K MWCO Tubing Specifications:
Membrane Type: Regenerated cellulose
Tubing Nominal Dry Thickness: 0.9 to 1.2mil (22 to 30 µM)
Glycerol Content: Trace
Sulfur Content: 0.1% to 0.15%
Heavy Metals Content: Trace

SnakeSkin Dialysis Tubing, 3.5K MWCO, is composed of regenerated-cellulose dialysis tubing and supplied as an open, pleated (telescoped) tube. SnakeSkin Dialysis Tubing is supplied in eight inch (20 cm) sticks containing 35 feet of dialysis tubing having a 16, 22, or 35mm circular internal diameter (I.D.). With similar capacity to 10.5 meters of 23 to 50 diameter dry flat width tubing, the hydrated SnakeSkin Dialysis Tubing holds ~2 to 10 mL of sample per centimeter of length.

The easy-open, pleated format of SnakeSkin Dialysis Tubing was developed to simplify large volume sample dialysis. To use, simply pull out and cut off the required length of tubing, fold over one end of tubing and apply a dialysis clip to close it, add sample at the open end and use a second clip to close the remaining end. Clips are sold separately. In comparison, many traditional dialysis tubing membranes that are supplied as dry, flattened rolls that are difficult to open and often require extensive soaking before they can be used.

The SnakeSkin membrane-pleating process does not change the tubing's MWCO. Also, any low MW contaminants present are removed during the dialysis process. Because SnakeSkin Dialysis Tubing is made from the same type of regenerated cellulose as flat tubing, its dialysis performance matches that of conventional tubing.

More Product Data
Separation characteristics of dialysis membranes

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SnakeSkin™ Dialysis Tubing, 3.5K MWCO, 22 mm
SnakeSkin™ Dialysis Tubing, 3.5K MWCO, 16 mm
SnakeSkin™ Dialysis Clips

Rapid Equilibrium Dialysis (RED) Device Inserts, 8K MWCO (Thermo Scientific™)

Thermo Scientific RED Device 8K MWCO Inserts are single-use, disposable inserts made up of two side-by-side chambers separated by a vertical cylinder of dialysis membrane in 8K MWCO, validated for minimal nonspecific binding.

Features of RED Device 8K MWCO Inserts:

Disposable: require no presoaking, assembly, or specialized equipment
Short incubation time: large dialysis surface area accelerates equilibrium
8K MWCO membrane: ideal molecular-weight cutoff for protein-drug binding studies
Membrane composition: regenerated cellulose with low glycerol content as a humectant

This format requires no extensive assembly steps or specialized equipment, and each chamber or well is easily accessible from the top of the insert after insertion in the base plate. Additionally, the high surface-to-volume ratio of the membrane compartment allows rapid dialysis, where equilibrium can be reached in 4 hours with high levels of reproducibility and accuracy. In many cases, experiments can be completed in less than 100 minutes.

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Rapid Equilibrium Dialysis (RED) Device Single-Use Plate with Inserts, 8K MWCO
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Rapid Equilibrium Dialysis (RED) Device Single-Use Base Plates
Rapid Equilibrium Dialysis (RED) Device Insert Removal Tool
Rapid Equilibrium Dialysis (RED) Device Single-Use Plate with Inserts, 12K MWCO

SnakeSkin™ Dialysis Tubing, 10K MWCO, 35 mm (Thermo Scientific™)

Thermo Scientific SnakeSkin Dialysis Tubing, 10K MWCO, is an easy and ready-to-use form of traditional dialysis membrane tubing that allows desalting and buffer exchange for 10 to 100 mL samples.

Features of SnakeSkin Dialysis Tubing, 10K MWCO, 35mm:

Convenient—ready to use, pre-wetted, pleated tube
High recovery—greater than 90% protein recovery
Speed—dialysis is generally completed in 4 to 6 hours
Stability—compatible with a variety of laboratory solutions, including acids, bases, hydrophobic solvents and alcohols

10K MWCO Tubing Specifications:
Membrane Type: Regenerated cellulose
Tubing Nominal Dry Thickness: 0.9 to 1.2mil (22 to 30 µM)
Glycerol Content: 21%
Sulfur Content: 0.1% to 0.15%
Heavy Metals Content: Trace

SnakeSkin Dialysis Tubing, 10K MWCO, is composed of regenerated-cellulose and supplied as an open, pleated (telescoped) tube. SnakeSkin Dialysis Tubing is supplied in eight inch (20 cm) sticks containing 35 feet of dialysis tubing having a 16, 22, or 35mm circular internal diameter (I.D.). With similar capacity to 10.5 meters of 23 to 50 diameter dry flat-width tubing, the hydrated SnakeSkin Dialysis Tubing holds ~2 to 10 mL of sample per centimeter of length.

The easy-open, pleated format of SnakeSkin Dialysis Tubing was developed to simplify large volume sample dialysis. To use, simply pull out and cut off the required length of tubing, fold over one end of tubing and apply a dialysis clip to close it, add sample at the open end and use a second clip to close the remaining end. Clips are sold separately. In comparison, many traditional dialysis tubing membranes that are supplied as dry, flattened rolls that are difficult to open and often require extensive soaking before they can be used.

The SnakeSkin membrane-pleating process does not change the tubing's MWCO. Also, any low MW contaminants present are removed during the dialysis process. Because SnakeSkin Dialysis Tubing is made from the same type of regenerated cellulose as flat tubing, its dialysis performance matches that of conventional tubing.

More Product Data
Separation characteristics of dialysis membranes

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SnakeSkin™ Dialysis Tubing, 10K MWCO, 22 mm
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Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 3 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.
The 3 mL size is convenient for many applications and the box of five is designed for the budget conscious laboratory

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ Dialysis Cassette Kit, 10K MWCO, 3 mL
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Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 30 mL

Slide-A-Lyzer™ Dialysis Flasks, 3.5K MWCO, 230 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Flasks, 3.5K MWCO, 230 mL facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules in sample volumes up to 230 mL.

Features of Slide-A-Lyzer Dialysis Flasks, 3.5K MWCO, 230 mL:

Easy to use – Simply pipette or pour sample into flask and begin dialysis
Fast dialysis – flat flask chamber with two membranes provides high surface-area to volume ratio, enabling dialysis of a 230 mL sample in 8 hours to overnight
High recovery – rectangular flask design maximizes recovery of entire sample volume via opening at top of flask
Multiple molecular-weight cuto-ffs – select the membrane MWCO that best suits your sample's molecular weight
Color-coded frames – easily identify membrane pore size (MWCO) based on the frame color

Dialysis Membrane Specifications:
• Composition: Regenerated-cellulose
• Thickness: 0.9 to 1.2mil (22.5 to 30μm); 2K MWCO = 2mil (50μm)
• Hydration Required Before Use: 30 seconds to 2 minutes
• Glycerol Content: Trace (10K MWCO content is 21%)
• Sulfur Content: 0.04 to 0.17%
• Heavy metals Content: Trace

Slide-A-Lyzer Dialysis Flasks are very easy to use. Add and remove sample using a serological pipette or by directly pouring the sample through a wide screw-cap opening at the top of the flask. No knots or clips are needed to seal the units. Slide-A-Lyzer Dialysis Flasks are manufactured using clean room conditions and have low-protein binding regenerated cellulose membranes to ensure maximum sample recovery and sample purity. The dialysis flasks are available in distinct colors, corresponding to the pore size (MWCO) of the dialysis membrane: Purple (20,000 daltons), Orange (10,000 daltons), Pink (3500 daltons), and Blue (2000 daltons).

Slide-A-Lyzer Dialysis Flasks are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of an inert plastic body. The membrane and flask materials are compatible with most laboratory buffers.

With Slide-A-Lyzer Dialysis Flasks, typical low-molecular weight contaminant removal, buffer exchange and desalting can be accomplished in 8 hours to overnight. The rate of dialysis depends on a variety of factors, including sample volume, size and shape of the molecule being dialyzed, agitation and temperature. Minimum dialysis time must be determined empirically for each specific system.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ G2 Dialysis Cassettes, gamma-irradiated, 10K MWCO, 3 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.

Features of Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO), gamma-irradiated:

Easy-loading—pipette-accessible for easy sample loading and retrieval
Self-floating—integrated air chambers eliminate the need for float buoys
Sturdy construction—ensures the highest possible sample integrity and protection
Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting
Gamma-irradiated—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination
Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes

10K Dialysis Membrane Specifications
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be loaded with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.

The 10K molecular-weight cutoff (MWCO) cassettes use a low-binding regenerated cellulose that is rated to completely retain proteins and other macromolecules that are larger than 10,000 daltons while allowing removal of buffer salts and small contaminants. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free. The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL.

Gamma-irradiated (Sterile) Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer G2 Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ Dialysis Cassettes, 3.5K MWCO, 12 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (3.5K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 3,500 daltons.

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

The 12 mL size is convenient for many preparative applications
Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ Dialysis Cassette Kit, 3.5K MWCO, 12 mL
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Slide-A-Lyzer™ Dialysis Cassettes, 2K MWCO, 0.5 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (2K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 2,000 daltons.
The 0.5 mL size is convenient for low volume samples

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

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SnakeSkin™ Dialysis Tubing, 7K MWCO, 22 mm (Thermo Scientific™)

Thermo Scientific SnakeSkin Dialysis Tubing, 7K MWCO, is an easy and ready-to-use form of traditional dialysis membrane tubing that allows desalting and buffer exchange for 15 to 100 mL samples.

Features of SnakeSkin Dialysis Tubing, 7K MWCO, 22mm:

Convenient—ready to use, pre-wetted, pleated tube
High recovery—greater than 90% protein recovery
Speed—dialysis is generally completed in 4 to 6 hours
Stability—compatible with a variety of laboratory solutions, including acids, bases, hydrophobic solvents and alcohols

7K MWCO Tubing Specifications:
Membrane Type: Regenerated cellulose
Tubing Nominal Dry Thickness: 0.9 to 1.2mil (22 to 30 µM)
Glycerol Content: 13%
Sulfur Content: 0.1% to 0.15%
Heavy Metals Content: Trace

SnakeSkin Dialysis Tubing, 7K MWCO, is composed of regenerated-cellulose and supplied as an open, pleated (telescoped) tube. SnakeSkin Dialysis Tubing is supplied in eight inch (20 cm) sticks containing 35 feet of dialysis tubing having a 22mm circular internal diameter (I.D.). Equivalent in capacity to 10.5 meters of 34 diameter dry flat width tubing, the hydrated SnakeSkin Dialysis Tubing holds ~3.7 mL of sample per centimeter of length.

The easy-open, pleated format of SnakeSkin Dialysis Tubing was developed to simplify large volume sample dialysis. To use, simply pull out and cut off the required length of tubing, fold over one end of tubing and apply a dialysis clip to close it, add sample at the open end and use a second clip to close the remaining end. Clips are sold separately. In comparison, many traditional dialysis tubing membranes that are supplied as dry, flattened rolls that are difficult to open and often require extensive soaking before they can be used.

The SnakeSkin membrane-pleating process does not change the tubing's MWCO. Also, any low MW contaminants present are removed during the dialysis process. Because SnakeSkin Dialysis Tubing is made from the same type of regenerated cellulose as flat tubing, its dialysis performance matches that of conventional tubing.

More Product Data
Separation characteristics of dialysis membranes

Related Products
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Slide-A-Lyzer™ Dialysis Cassette Kit, 10K MWCO, 3 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.
The 3 mL size is convenient for many applications and the kit format contains syringes, needles, and float buoys for convenience.

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 30 mL

Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 12 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.
The 12 mL size is convenient for preparative applications and gamma irradiation assures sterility.

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 0.5 mL
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Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 30 mL

Slide-A-Lyzer™ Dialysis Flasks, 10K MWCO, 250 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Flasks, 10K MWCO, 250 mL facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules in sample volumes up to 250 mL.

Features of Slide-A-Lyzer Dialysis Flasks, 10K MWCO, 250 mL:

Easy to use – Simply pipette or pour sample into flask and begin dialysis
Fast dialysis – flat flask chamber with two membranes provides high surface-area to volume ratio, enabling dialysis of a 250 mL sample in 8 hours to overnight
High recovery – rectangular flask design maximizes recovery of entire sample volume via opening at top of flask
Multiple molecular-weight cut-offs – select the membrane MWCO that best suits your sample's molecular weight
Color-coded frames – easily identify membrane pore size (MWCO) based on the frame color

Dialysis Membrane Specifications:
• Composition: Regenerated-cellulose
• Thickness: 0.9 to 1.2mil (22.5 to 30μm); 2K MWCO = 2mil (50μm)
• Hydration Required Before Use: 30 seconds to 2 minutes
• Glycerol Content: Trace (10K MWCO content is 21%)
• Sulfur Content: 0.04 to 0.17%
• Heavy metals Content: Trace

Slide-A-Lyzer Dialysis Flasks are very easy to use. Add and remove sample using a serological pipette or by directly pouring the sample through a wide screw-cap opening at the top of the flask. No knots or clips are needed to seal the units. Slide-A-Lyzer Dialysis Flasks are manufactured using clean room conditions and have low-protein binding regenerated cellulose membranes to ensure maximum sample recovery and sample purity. The dialysis flasks are available in distinct colors, corresponding to the pore size (MWCO) of the dialysis membrane: Purple (20,000 daltons), Orange (10,000 daltons), Pink (3500 daltons), and Blue (2000 daltons).

Slide-A-Lyzer Dialysis Flasks are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of an inert plastic body. The membrane and flask materials are compatible with most laboratory buffers.

With Slide-A-Lyzer Dialysis Flasks, typical low-molecular weight contaminant removal, buffer exchange and desalting can be accomplished in 8 hours to overnight. The rate of dialysis depends on a variety of factors, including sample volume, size and shape of the molecule being dialyzed, agitation and temperature. Minimum dialysis time must be determined empirically for each specific system.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ Dialysis Flotation Disks

Slide-A-Lyzer™ G2 Dialysis Cassettes, 10K MWCO, 70 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.

Features of Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO):

Easy-loading—pipette-accessible for easy sample loading and retrieval
Self-floating—integrated air chambers eliminate the need for float buoys
Sturdy construction—ensures the highest possible sample integrity and protection
Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting
Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes

10K Dialysis Membrane Specifications
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be loaded with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.

The 10K molecular-weight cutoff (MWCO) cassettes use a low-binding regenerated cellulose that is rated to completely retain proteins and other macromolecules that are larger than 10,000 daltons while allowing removal of buffer salts and small contaminants. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free. The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL.

Gamma-irradiated (Sterile) Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer G2 Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ G2 Dialysis Cassettes, 10K MWCO, 15 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 10K MWCO, 30 mL

Slide-A-Lyzer™ G2 Dialysis Cassettes, gamma-irradiated, 10K MWCO, 15 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.

Features of Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO), gamma-irradiated:

Easy-loading—pipette-accessible for easy sample loading and retrieval
Self-floating—integrated air chambers eliminate the need for float buoys
Sturdy construction—ensures the highest possible sample integrity and protection
Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting
Gamma-irradiated—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination
Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes

10K Dialysis Membrane Specifications
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be loaded with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.

The 10K molecular-weight cutoff (MWCO) cassettes use a low-binding regenerated cellulose that is rated to completely retain proteins and other macromolecules that are larger than 10,000 daltons while allowing removal of buffer salts and small contaminants. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free. The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL.

Gamma-irradiated (Sterile) Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer G2 Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ Dialysis Cassettes, 3.5K MWCO, 30 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (3.5K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 3,500 daltons.
The 30 mL size is convenient for many large-scale applications

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

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