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eZKine™ Th17/Th22 Whole Blood Intracellular Cytokine Kit (Invitrogen™)

This eZKine Th17/Th22 Whole Blood Intracellular Cytokine Kit is designed to rapidly identify cytokine producing T lymphocytes of the Th17 and Th22 lineages after stimulation of whole peripheral blood samples. Stimulation of whole blood in the presence of Protein Transport Inhibitor Cocktail (cat. 00-4980, not included) is followed by red blood cell lysis and fixation in a single step and subsequent permeabilization. Samples are then ready to stain with the Th17/Th22 cocktail and concentration-matched Isotype Control cocktail.

Th17 cells play a key role in barrier defense against extracellular pathogens such as bacteria and fungi and play a significant role in autoimmune disease. They are defined by their expression of the transcription factor ROR gamma t and the inflammatory cytokine IL-17A. Through their activation and subsequent cytokine production, Th17 cells trigger pro-inflammatory signaling that promotes neutrophil mobilization and the expression of antimicrobial peptides. Some plasticity between the Th1 and Th17 lineages has been reported. Furthermore, a pathogenic Th17 subpopulation has been described with expression of both IL-17 and IFN gamma, as well as ROR gamma t and T-bet. These cells are implicated in several inflammatory and autoimmune diseases.

Th22 cells are a CD4+ cell subset dedicated to the production of IL-22, an IL-10 family member. They are characterized by the expression of IL-22, IL-13, fibroblast growth factor (FGF) and other factors involved in tissue remodeling, but not IFN gamma, IL-4, or IL-17. IL-22 cells elicit tissue repair and wound healing responses from non-hematopoietic cells such as keratinocytes, myofibroblasts and epithelial cells. Th22 cells may also play a pathogenic role in many inflammatory diseases such as asthma, atopic dermatitis, psoriasis, rheumatoid arthritis, scleroderma, Crohn’s disease and uveitis.

Reactivity/Species
Human

Reported Application
Intracellular Staining Followed by Flow Cytometric Analysis

eZKine™ CD8 Activation 2 Whole Blood Intracellular Cytokine Kit (Invitrogen™)

This eZKine CD8 Activation 2 Whole Blood Intracellular Cytokine Kit is designed to rapidly identify cytokine producing T lymphocytes of the CD8+ T cell lineage after stimulation of whole peripheral blood samples. Stimulation of whole blood in the presence of Protein Transport Inhibitor Cocktail (cat. 00-4980, not included) is followed by red blood cell lysis and fixation in a single step and subsequent permeabilization. Samples are then ready to stain with the CD8 Activation 2 cocktail and concentration-matched Isotype Control cocktail.

CD8+ T cells, or cytotoxic T lymphocytes, are immune cells that are responsible for the detection and clearance of virally or bacterically infected host cells. Upon recognition of antigen displayed on the surface of infected cells, activated CD8+ T cells upregulate markers of early activation such as CD69 and FAS ligand, followed by the release of granules containing granzymes and perforin as well as secretion of cytokines such as IFN gamma and TNF alpha. Granzymes and perforin act in concert to directly lyse infected target cells, while cytokines have paracrine effects including the recruitment and activation of macrophages, lymphocytes, and PMNs. IFN gamma is important for the upregulation of MHC class I and II on nearby cells as well as promoting Th1 cell differentiation.

Reactivity/Species
Human

Reported Application
Flow Cytometric Analysis

eZKine™ Th1 Activation 1 Whole Blood Intracellular Cytokine Kit (Invitrogen™)

This eZKine Th1 Activation 1 Whole Blood Intracellular Cytokine Kit is designed to rapidly identify cytokine producing T lymphocytes of the Th1 lineage after stimulation of whole peripheral blood samples. Stimulation of whole blood in the presence of Protein Transport Inhibitor Cocktail (cat. 00-4980, not included) is followed by red blood cell lysis and fixation in a single step and subsequent permeabilization. Samples are then ready to stain with the Th1 Activation 1 cocktail and concentration-matched Isotype Control cocktail.

Th1 cells are a CD4+ T cell subset that plays an important role in host defense against intracellular bacteria and viruses. Th1 lineage commitment is controlled by the transcription factor, T-bet, and these cells are a primary source of IFN gamma, which stimulates macrophages, lymphocytes, and PMNs in the destruction of bacterial pathogens. IFN gamma also helps foster the development of cytotoxic lymphocytes (CTL & NK cells) that are responsible for the cell-mediated immune response against viruses and tumor cells. Dysregulation of the Th1 response plays a critical role in many inflammatory and autoimmune diseases.

Reactivity/Species
Human

Reported Application
Intracellular Staining Followed by Flow Cytometric Analysis

eZKine™ CD8 Activation 1 Whole Blood Intracellular Cytokine Kit (Invitrogen™)

This eZKine CD8 Activation 1 Whole Blood Intracellular Cytokine Kit is designed to rapidly identify cytokine producing T lymphocytes of the CD8+ T cell lineage after stimulation of whole peripheral blood samples. Stimulation of whole blood in the presence of Protein Transport Inhibitor Cocktail (cat. 00-4980, not included) is followed by red blood cell lysis and fixation in a single step and subsequent permeabilization. Samples are then ready to stain with the CD8 Activation 1 cocktail and concentration-matched Isotype Control cocktail.

CD8+ T cells, or cytotoxic T lymphocytes, are immune cells that are responsible for the detection and clearance of virally or bacterically infected host cells. Upon recognition of antigen displayed on the surface of infected cells, activated CD8+ T cells upregulate markers of early activation such as CD69 and FAS ligand, followed by the release of granules containing granzymes and perforin as well as secretion of cytokines such as IFN gamma and TNF alpha. Granzymes and perforin act in concert to directly lyse infected target cells, while cytokines have paracrine effects including the recruitment and activation of macrophages, lymphocytes, and PMNs. IFN gamma is important for the upregulation of MHC class I and II on nearby cells as well as promoting Th1 cell differentiation.

Reactivity/Species
Human

Reported Application
Intracellular Staining Followed by Flow Cytometric Analysis

eZKine™ 4-Color Compensation Control Kit (Invitrogen™)

This eZKine™ 4-Color Compensation Control Kit is designed for compensation setup with eZKine™ Whole Blood Intracellular Cytokine Kits. It contains the necessary components to create single-color compensation controls for use with eZKine™ cocktails, including OneComp eBeads and 4 vials of antibody representing each of the 4 fluorochromes used in eZKine™ cocktails. OneComp eBeads can be stained with each of the 4 fluorochrome-conjuated antibodies to set up single-color compensation control tubes.

Reactivity/Species
Human

Reported Application
Flow Cytometric Analysis

eZKine™ Th1/Th17 Whole Blood Intracellular Cytokine Kit (Invitrogen™)

This eZKine Th1/Th17 Whole Blood Intracellular Cytokine Kit is designed to rapidly identify cytokine producing T lymphocytes of the Th1 and Th17 lineages after stimulation of whole peripheral blood samples. Stimulation of whole blood in the presence of Protein Transport Inhibitor Cocktail (cat. 00-4980, not included) is followed by red blood cell lysis and fixation in a single step and subsequent permeabilization. Samples are then ready to stain with the Th1/Th17 cocktail and concentration-matched Isotype Control cocktail.

Th1 cells are a CD4+ T cell subset that plays an important role in host defense against intracellular bacteria and viruses. Th1 lineage commitment is controlled by the transcription factor, T-bet, and these cells are a primary source of IFN gamma, which stimulates macrophages, lymphocytes, and PMNs in the destruction of bacterial pathogens. IFN gamma also helps foster the development of cytotoxic lymphocytes (CTL & NK cells) that are responsible for the cell-mediated immune response against viruses and tumor cells. Dysregulation of the Th1 response plays a critical role in many inflammatory and autoimmune diseases.

Th17 cells play a key role in barrier defense against extracellular pathogens such as bacteria and fungi and play a significant role in autoimmune disease. They are defined by their expression of the transcription factor ROR gamma t and the inflammatory cytokine IL-17A. Through their activation and subsequent cytokine production, Th17 trigger pro-inflammatory signaling that promotes neutrophil mobilization and the expression of antimicrobial peptides. Some plasticity between the Th1 and Th17 lineages has been reported. Furthermore, a pathogenic Th17 subpopulation has been described with expression of both IL-17 and IFN gamma, as well as ROR gamma t and T-bet. These cells are implicated in several inflammatory and autoimmune diseases.

Reactivity/Species
Human

Reported Application
Intracellular Staining Followed by Flow Cytometric Analysis