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Recovery™ Cell Culture Freezing Medium (Gibco™)

Recovery™ Cell Culture Freezing Medium is a complete, ready-to-use freezing medium for cryopreservation of a wide variety of mammalian cells, including CHO-S, CHO-K1, HEK 293, Jurkat, and NIH 3T3. Recovery™ Cell Culture Freezing Medium features:

• Classic formulation optimized for better performance
• Suitability for a wide variety of mammalian cells
• Quality and performance tested

Classic formulation optimized for better performance
For many years, researchers have used a common recipe for making freezing medium: High-glucose Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% serum and 10% DMSO. Recovery™ Cell Culture Freezing Medium is an optimized version of this classic formulation, using high-quality Gibco® Bovine Serum and Fetal Bovine Serum to provide improved cell recovery and viability after thawing.

Suitability for a wide variety of mammalian cells
Recovery™ Cell Culture Freezing Medium can be used to freeze most mammalian cells cultured in DMEM, DMEM/F-12, MEM, RPMI 1640, and Ham's F-12, including CHO-S, CHO-K1, HEK 293, Jurkat, and NIH 3T3 cells.

Quality and performance tested
Recovery™ Cell Culture Freezing Medium is quality tested for pH, osmolality, sterility, and endotoxin. In addition, each lot is performance tested using CHO-K1 cells.

cGMP manufacturing and quality system
Recovery™ Cell Culture Freezing Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is also registered with the FDA as a medical device manufacturer and certified to ISO 13485 standards.

Synth-a-Freeze™ Cryopreservation Medium (Gibco™)

Synth-a-Freeze® is a defined, liquid cryopreservation medium containing 10% dimethylsulfoxide (DMSO) without antibiotics, antimycotics, hormones, growthfactors, serum or protein and buffered with HEPES and sodium bicarbonate. Synth-a-Freeze is intended for freezing and storing a variety of mammalian cell types including human keratinocytes, embryonic stem cells, neural stem cells and mesenchymal stem cells. It should not be used for melanocytes.

CTS™ Synth-a-Freeze™ Medium (Gibco™)

CTS™ (Cell Therapy Systems) Synth-a-Freeze® medium is a chemically defined, protein-free liquid cryopreservation medium intended for freezing and storing a variety of mammalian cell types including stem cells. Synth-a-Freeze® CTS™ media is optimally formulated without antibiotics, antimycotics, hormones, growth factors, serum, or protein and buffered with HEPES and sodium bicarbonate.

Use for cryopreserving a variety of primary and stem cells

• Save time and effort with 4 degree stable storage of thawed media and compatibility with a variety freezing protocols
• Be ready for clinical research with this cGmP manufactured, chemically defined, protein-free formula

Cryopreserve a Variety of Cell Types
Cell Therapy Systems Synth-a-Freeze® CTS™ media performs comparable to our standard, serum-containing cryopreservation medium for cryopreserving a variety of cell types including human Embryonic Stem Cells, Neural Stem Cells (Ref 1), Mesenchymal Stem Cells and Keratinocytes (Ref 2). We do not recommend using Synth-a-Freeze® media for cryopreservation of human epidermal melanocytes.

Simple Incorporation into Your Current Freezing Protocol
Synth-a-Freeze® CTS™ media can be used with any standard freezing protocol. Just add thawed Cell Therapy Systems Synth-a-Freeze® CTS™ media to detached and pelleted cells, aliquot, and freeze. For optimal results, the use of a controlled-rate freezer is recommended.

Simplify Your Transition into Clinical Research
The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco® Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)

For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products.

CAUTION: Not intended for direct administration into humans or animals

References:
• S. Shin and M. Vemuri. Culture and Differentiation of Human Neural Stem Cells. L.C. Doering (ed). Protocols for Neural Cell Culture. Springer Protocol Handbooks.
• S. Lemaire, K. Kosowska-Shick, P. Appelbaum, G. Verween, P. Tulkens, and F. Van Bambeke. Cellular Pharmacodynamics of the Novel Biaryloxazolidinone Radezolid: Studies with Infected Phagocytic and Nonphagocytic cells, Using Staphylococcus aureus, Staphylococcus epidermidis, Listeria monocytogenes, and Legionella pneumophila. Antimicrob Agents Chemother 54(6) 2010: 2549-2559.