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Regulatory statements vary by product. Please see product-specific literature to determine product use.

What is the difference between GlutaMAX Supplement (Cat. No. 35050061), CTS GlutaMAX-I Supplement (Cat. No. A1286001), and CTS GlutaMAX Supplement (Cat. No. A4737001)? Product FAQ

Answer

The formulation and manufacturing process are the same for all three products. The main difference is in the “intended use” statement. CTS GlutaMAX-I Supplement (Cat. No. A1286001) and CTS GlutaMAX Supplement (Cat. No. A4737001) have an intended use statement of “For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals”. GlutaMAX Supplement (Cat. No. 35050061) has an intended use statement of “For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals”. The catalog number to use will depend on the specific application.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E17495

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What is the difference between Gibco GlutaMAX and glutamine supplements? Product FAQ

Answer

GlutaMAX-I supplement is an improved cell culture supplement that can be used as a direct substitute for L-glutamine in your cell culture medium. Please go here to read more about the properties of GlutaMAX supplement (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/media-supplements/glutamax-media/glutamax-vs-glutamine.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E11884

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Will the media formulations containing GlutaMAX supplement change with respect to L-glutamine content? Product FAQ

Answer

In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E11918

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Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture? Product FAQ

Answer

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E11919

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Do my cells need to adapt to GlutaMAX Supplement (Cat. No. 35050061, 35050079)? Product FAQ

Answer

Some cell lines may require an adaptation period, and the culture may lag in growth until it becomes accustomed to GlutaMAX Supplement (Cat. No. 3050061, 35050079). For serum-free media it is best to add L-glutamine along with GlutaMAX supplement. We don’t recommend that you use GlutaMAX supplement with insect cells such as Sf9.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E21462

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Why is GlutaMAX Supplement (Cat. Nos. 35050061, 35050079) recommended over L-glutamine when reconstituting Geltrex Reduced Growth Factor Basement Membrane Matrix? Product FAQ

Answer

GlutaMAX Supplement (Cat. No. 35050061, 35050079) is highly recommended to reconstitute Geltrex Reduced Growth Factor Basement Membrane Matrix because it offers higher stability than L-glutamine. The L-glutamine in Geltrex matrix comes in the form of the dipeptide L-alanyl-L-glutamine, which degrades slower than L-glutamine in cell culture. While GlutaMAX supplement is highly recommended, it is not required for Geltrex matrix–based culture systems.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E22560

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Do both the DMEM, high glucose, GlutaMAX Supplements, Cat. No. 61965026 and 10566016, have the same formulation? Product FAQ

Answer

Yes, both the DMEM, high glucose, GlutaMAX Supplements, Cat. No. 61965026 and 10566016, have the same formulation.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Answer Id: E21397

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Does Dynamis AGT Medium (Cat. Nos. A2617501, A2617502, A2617503, A2617504) require supplementation for use? Product FAQ

Answer

When using Dynamis AGT Medium (Cat. Nos. A2617501, A2617502, A2617503, A2617504) for culturing a CHO cell line other than the glutamine synthetase (GS) gene expression system (GS-CHO expression system), the addition of 2-8 mM L-glutamine or a similar amount of GlutaMAX Supplement (Cat. No. 35050079, 35050061) is recommended.

Note: We recommend that you supplement with glucose as needed so that glucose levels don’t fall below 2g/L in culture (see the product User Guide for more details).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E21685

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Is there an alternative for L15 Leibovitz Medium (Cat. Not. 11415056)? Product FAQ

Answer

The alternative for L15 Leibovitz Medium (Cat. No. 11415056) is Lebovitz's L-15 Medium, GlutaMAX Supplement (Cat. No. 31415029).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E21941

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What are the components of the StemPro hESC SFM kit? Product FAQ

Answer

The StemPro hESC SFM kit (Cat. No. A1000701) contains: - 500 mL DMEM/F-12 with GlutaMax (Cat. No. 10565-018)
- 10 mL StemPro hESC SFM Supplement 50X (Cat. No. A10006-01)
- 40 mL Bovine Serum Albumin 25% (BSA Solution) (Cat. No. A10008-01)
Note that the StemPRO hESC Supplement and BSA solution cannot be ordered individually.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E5296

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How do I prepare the StemPRO hESC SFM complete medium? Product FAQ

Answer

We recommend to thaw the supplement in the 37°C water bath, aliquot and freeze at -5°C to -20°C. Thaw each aliquot only one additional time. To make 100 ml complete medium, mix 90.8 ml DMEM/F-12 with GlutaMax matrix, 2 ml StemPRO hESC supplement, 7.2 ml 25% BSA solution, 80 ul bFGF (at 10 ug/ml), and 182 ul 2-mercaptoethanol (at 55mM). Store at 2°C-8°C until ready to use, but use the same day.

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Answer Id: E5304

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What cell lines has StemPRO hESC SFM been tested with? Product FAQ

Answer

StemPro hESC SFM has been tested with the following hESC: H1, H9, BG01, BG02, BG03, HUES6, HUES9, Cyth203, CyT49, BG01v, HES-2, HES-3, KhES-1, and TE06. The medium has also been used with the Rhesus monkey line R336.4. Some cells may require a short adaptation time but most do not.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E5300

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Can StemPRO hESC SFM be used for new cell line derivations? Product FAQ

Answer

Yes, derivations using StemPro hESC SFM have been carried out using either a basement membrane extract (like Geltrex growth factor) or feeder cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E5301

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Does StemPRO hESC SFM require the use of a basement membrane matrix? Product FAQ

Answer

Yes, we recommend using Geltrex hESC-qualified Reduced Growth Factor basement membrane (catalog number A10480-02), CELLstart (catalog number A10142-01) or similar matrix.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E5299

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What additional products do I need to make a complete StemPRO hESC SFM? Product FAQ

Answer

In addition to the StemPRO hESC SFM kit, you will also need the following to make a complete medium: full length recombinant human FGF (catalog # PHG0261) and 2-Mercaptoethanol (catalog # 21985-023).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E5297

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