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Lab Vision™ Epidermal Growth Factor Receptor/EGFR Ab-13, Mouse Monoclonal Antibody (Thermo Scientific™)

Obtain accurate, reproducible results in immunoprecipitation experiments with Thermo Scientific™ Epidermal Growth Factor Receptor/EGFR Ab-13, Mouse Monoclonal Antibody.

Lab Vision™ Vascular Endothelial Growth Factor (VEGF), Rabbit Polyclonal Antibody (Thermo Scientific™)

Take advantage of one marker in a comprehensive antibody portfolio that addresses all major disease states with the Thermo Scientific™ Vascular Endothelial Growth Factor (VEGF), Rabbit Polyclonal Antibody.

pHrodo™ Red Epidermal Growth Factor (EGF) Conjugate (Invitrogen™)

pHrodo Red Epidermal Growth Factor (EGF) Conjugate is used for the detection of internalized EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. Fluorescent labels that respond to a pH change add another level of detection. pHrodo Red is weakly fluorescent outside of cells at neutral pH, but when internalized specifically through EGF endocytosis, it becomes brightly fluorescent in the acidic endosomes. This allows the measurement of internalized EGF, as well as the dynamic monitoring of EGF internalization in live cells.

Features of this EGF conjugate include:

• pHrodo Red dye conjugated directly to highly active human recombinant EGF molecule
• Excitation/emission of 560/585 nm
• Excellent photostability
• Appropriate for multiplexing with green fluorophores such as GFP, FITC, and Alexa Fluor 488

pHrodo Red Epidermal Growth Factor (EGF) Conjugate is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometery, and microplate-based fluorimetry or high throughput screening (HTS). This reagent is also compatible with various benchtop instruments such as the Evos, FLoid, Tali, and Attune instruments.

Geltrex™ LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Gibco™)

Geltrex™ LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors and is free of viruses, including lactose dehydrogenase elevating virus (LDEV), sometimes found in this type of preparation. Routinely used for attachment and maintenance of human embryonic stem cells (hESC), every lot of Geltrex™ LDEV-free hESC-qualified Reduced Growth Factor Basement Membrane Matrix is quality control tested using hESCs and StemPro hESC Serum Free Media to help ensure growth and pluripotency of each lot.

Key Features of Geltrex™ LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix:

• Every lot is qualified using hESC, which helps reduce the need for customers to lot screen
• Free of the lactose dehydrogenase elevating virus (LDEV) making it safe for all types of cell culture
• Consistent protein concentration lot-to-lot helps ensure reproducible results

Gain Reliability and Consistency in Your hESC Studies
Human ESCs have specific needs for attachment and maintenance and some lots of basement membrane extract may not provide adequate support. Geltrex™ hESC-qualified reduced growth factor basement membrane matrix has been tested for support of hESC growth and pluripotency, therefore minimizing the need for you to test multiple lots to find one that works. Our consistent manufacturing process helps ensure a protein concentration averaging 15 mg/ml with very little variation from lot-to-lot, so you can have confidence in every purchase.

Free of LDEV Contamination
Recent advances in processing EHS tumor cells have resulted in virus free basement membrane extracts. Every lot is tested by tested by RT-PCR methods so you can now have confidence that every lot of Geltrex™ is virus-free.

Epidermal Growth Factor, Biotin-XX Conjugate (biotin EGF) (Invitrogen™)

This purified epidermal growth factor (EGF) contains a single biotin-XX molecule on the N-terminal amino acid. Biotin-XX EGF contains a long spacer arm that enhances the probe’s affinity for the EGF receptor and facilitates binding of a label such as a dye–streptavidin molecule, a Qdot® nanocrystals–streptavidin molecule, or an enzyme-conjugated streptavidin. Labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more.

Epidermal Growth Factor Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Number of biotin-XX molecules on each EGF molecule: 1


Find Fluorescent EGF Conjugates
In addition to biotinylated EGF, we offer biotinylated EGF precomplexed to selected fluorescent streptavidin labels and EGF directly labeled with some fluorescent dyes. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 555 Streptavidin (Alexa Fluor™ 555 EGF complex) (Invitrogen™)

We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green® 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor® dyes and Texas Red® dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor.

Epidermal Growth Factor Conjugate Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Biotin-XX complexed with Alexa Fluor® 555 streptavidin (~555/565 nm)
• Number of fluorophore molecules on each EGF molecule: ~2–3
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter


Find More Receptor Binding and Phagocytosis Probes
We offer a number of fluorescently labeled probes for studying receptor-mediated endocytosis, membrane markers for endocytosis and exocytosis, and methods for detecting internalized fluorescent ligands. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

pHrodo™ Green Epidermal Growth Factor (EGF) Conjugate (Invitrogen™)

pHrodo Green Epidermal Growth Factor (EGF) Conjugate is used for the detection of internalized EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. Fluorescent labels that respond to a pH change add another level of detection. pHrodo Green is weakly fluorescent outside of cells at neutral pH, but when internalized specifically through EGF endocytosis, it becomes brightly fluorescent in the acidic endosomes. This allows the measurement of internalized EGF, as well as the dynamic monitoring of EGF internalization in live cells.

Features of this EGF conjugate include:

• pHrodo Green dye conjugated directly to highly active human recombinant EGF molecule
• Excitation/emission of 509/533 nm
• Excellent photostability
• Appropriate for multiplexing with red and deep red fluorophores such as RFP, Alexa Fluor 594, MitoTracker Red, and MitoTracker Deep Red

pHrodo Green Epidermal Growth Factor (EGF) Conjugate is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometery, and microplate-based fluorimetry or high throughput screening (HTS). This reagent is also compatible with various benchtop instruments such as the Evos, FLoid, Tali, and Attune instruments.

Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Gibco™)

Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors. This pre-diluted version of the matrix can be taken directly from the refrigerator and dispensed into cell culture plates without further handling. Geltrex hESC-Qualified Ready-To-Use basement membrane matrix is optimized for stem cell culture applications using StemPro hESC SFM. For stem cell culture using the StemFlex Medium or Essential 8 Media System, use Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix.

Geltrex hESC-Qualified Ready-To-Use matrix is:

• Consistent—pre-dilution at a concentration of 1:100 ensures optimal protein concentration for stem cell attachment to standard cell culture plates
• Stable—storage at 4°C eliminates overnight thawing on ice
• Cost effective— the ready-to-use format saves multiple preparation steps and potential for product failure

Reduced variability
Geltrex hESC-Qualified Ready-To-Use matrix is provided as a 1:100 dilution in a buffered solution that can be added directly to cell culture plates for coating. Each lot is prepared at the appropriate protein concentration for optimal stem cell attachment, which minimizes the need to calculate dilution schemes for every lot of matrix.

Reliable
Designed for 4°C storage for the life of the product, this matrix can be kept in the refrigerator along with your cell culture media—no more overnight thawing in clumsy ice baths and no risk of premature gelling. In addition, every lot is functionally qualified using human embryonic stem cells and tested to ensure it is free of lactose dehydrogenase elevating virus (LDEV), making it safe for all cell culture labs.

Cost effective
Geltrex hESC-Qualified Ready-To-Use matrix eliminates multiple steps—no more freezing, thawing, diluting, and aliquoting. This product is a true time saver.

Lab Vision™ Vascular Endothelial Growth Factor (VEGF) Ab-3, Mouse Monoclonal Antibody (Thermo Scientific™)

Provide accurate, reproducible results with the Thermo Scientific™ Vascular Endothelial Growth Factor (VEGF) Ab-3, Mouse Monoclonal Antibody.

Lab Vision™ Epidermal Growth Factor Receptor/EGFR Ab-15, Mouse Monoclonal Antibody (Thermo Scientific™)

Ensure accurate, reproducible results in western blotting and immunoprecipitation experiments.

Epidermal Growth Factor, Fluorescein Conjugate (Fluorescein EGF) (Invitrogen™)

We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green® 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor® dyes and Texas Red® dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor.

Epidermal Growth Factor Conjugate Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Fluorescein (~494/518 nm)
• Number of fluorophore molecules on each EGF molecule: 1
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter


Find More Receptor Binding and Phagocytosis Probes
We offer a number of fluorescently labeled probes for studying receptor-mediated endocytosis, membrane markers for endocytosis and exocytosis, and methods for detecting internalized fluorescent ligands. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 647 Streptavidin (Alexa Fluor™ 647 EGF complex) (Invitrogen™)

We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green® 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor® dyes and Texas Red® dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor.

Epidermal Growth Factor Conjugate Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Biotin-XX complexed with Alexa Fluor® 647 streptavidin (~650/665 nm)
• Number of fluorophore molecules on each EGF molecule: ~2–3
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter


Find More Receptor Binding and Phagocytosis Probes
We offer a number of fluorescently labeled probes for studying receptor-mediated endocytosis, membrane markers for endocytosis and exocytosis, and methods for detecting internalized fluorescent ligands. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

Lab Vision™ Epidermal Growth Factor Receptor/EGFR Ab-10, Mouse Monoclonal Antibody (Thermo Scientific™)

Ensure accurate, reproducible results in immunohistochemistry, western blotting and immunoprecipitation experiments.

Lab Vision™ Epidermal Growth Factor Receptor/EGFR Ab-2, Mouse Monoclonal Antibody (Thermo Scientific™)

Ensure accurate, reproducible results in immunoprecipitation experiments with Thermo Scientific™ Epidermal Growth Factor Receptor/EGFR Ab-2, Mouse Monoclonal Antibody.

Lab Vision™ Epidermal Growth Factor Receptor/EGFR Ab-11, Mouse Monoclonal Antibody (Thermo Scientific™)

Obtain accurate, reproducible results in western blotting and immunoprecipitation experiments.