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Lab Vision™ Vascular Endothelial Growth Factor (VEGF), Rabbit Polyclonal Antibody (Thermo Scientific™)

Take advantage of one marker in a comprehensive antibody portfolio that addresses all major disease states with the Thermo Scientific™ Vascular Endothelial Growth Factor (VEGF), Rabbit Polyclonal Antibody.

Lab Vision™ Vascular Endothelial Growth Factor (VEGF), Rabbit Monoclonal Antibody (Thermo Scientific™)

Employ part of a comprehensive antibody portfolio with the Thermo Scientific™ Vascular Endothelial Growth Factor, Rabbit Monoclonal Antibody. Studies show rabbit monoclonal antibodies are more sensitive and specific than other available markers.

Lab Vision™ Transforming Growth Factor α (TGFα) Ab-1, Mouse Monoclonal Antibody (Thermo Scientific™)

Provide accurate, reproducible results with the Thermo Scientific™ Transforming Growth Factor α (TGFα) Ab-1, Mouse Monoclonal Antibody.

Hepatocyte Growth Factor Ab-2, Mouse Monoclonal Antibody (Thermo Scientific™)

Ideal for Western Blotting, Thermo Scientific™ Hepatocyte Growth Factor Ab-2, Mouse Monoclonal Antibody provides accurate, reproducible results.

Epidermal Growth Factor, Biotin-XX Conjugate (biotin EGF) (Invitrogen™)

This purified epidermal growth factor (EGF) contains a single biotin-XX molecule on the N-terminal amino acid. Biotin-XX EGF contains a long spacer arm that enhances the probe’s affinity for the EGF receptor and facilitates binding of a label such as a dye–streptavidin molecule, a Qdot® nanocrystals–streptavidin molecule, or an enzyme-conjugated streptavidin. Labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more.

Epidermal Growth Factor Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Number of biotin-XX molecules on each EGF molecule: 1


Find Fluorescent EGF Conjugates
In addition to biotinylated EGF, we offer biotinylated EGF precomplexed to selected fluorescent streptavidin labels and EGF directly labeled with some fluorescent dyes. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Lab Vision™ Epidermal Growth Factor Receptor/EGFR Ab-5, Mouse Monoclonal Antibody (Thermo Scientific™)

Obtain accurate, reproducible results in immunohistochemistry, western blotting, immunofluorescence, immunoprecipitation and flow cytometry experiments.

Geltrex™ LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Gibco™)

Geltrex™ LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors and is free of viruses, including lactose dehydrogenase elevating virus (LDEV), sometimes found in this type of preparation. Routinely used for attachment and maintenance of human embryonic stem cells (hESC), every lot of Geltrex™ LDEV-free hESC-qualified Reduced Growth Factor Basement Membrane Matrix is quality control tested using hESCs and StemPro hESC Serum Free Media to help ensure growth and pluripotency of each lot.

Key Features of Geltrex™ LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix:

• Every lot is qualified using hESC, which helps reduce the need for customers to lot screen
• Free of the lactose dehydrogenase elevating virus (LDEV) making it safe for all types of cell culture
• Consistent protein concentration lot-to-lot helps ensure reproducible results

Gain Reliability and Consistency in Your hESC Studies
Human ESCs have specific needs for attachment and maintenance and some lots of basement membrane extract may not provide adequate support. Geltrex™ hESC-qualified reduced growth factor basement membrane matrix has been tested for support of hESC growth and pluripotency, therefore minimizing the need for you to test multiple lots to find one that works. Our consistent manufacturing process helps ensure a protein concentration averaging 15 mg/ml with very little variation from lot-to-lot, so you can have confidence in every purchase.

Free of LDEV Contamination
Recent advances in processing EHS tumor cells have resulted in virus free basement membrane extracts. Every lot is tested by tested by RT-PCR methods so you can now have confidence that every lot of Geltrex™ is virus-free.

Epidermal Growth Factor, Fluorescein Conjugate (Fluorescein EGF) (Invitrogen™)

We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green® 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor® dyes and Texas Red® dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor.

Epidermal Growth Factor Conjugate Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Fluorescein (~494/518 nm)
• Number of fluorophore molecules on each EGF molecule: 1
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter


Find More Receptor Binding and Phagocytosis Probes
We offer a number of fluorescently labeled probes for studying receptor-mediated endocytosis, membrane markers for endocytosis and exocytosis, and methods for detecting internalized fluorescent ligands. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

Lab Vision™ Vascular Endothelial Growth Factor (VEGF) Ab-7, Mouse Monoclonal Antibody (Thermo Scientific™)

Provide accurate, reproducible results with the Thermo Scientific™ Vascular Endothelial Growth Factor (VEGF) Ab-7, Mouse Monoclonal Antibody.

Lab Vision™ Epidermal Growth Factor Receptor/EGFR Ab-15, Mouse Monoclonal Antibody (Thermo Scientific™)

Ensure accurate, reproducible results in western blotting and immunoprecipitation experiments.

Lab Vision™ Epidermal Growth Factor Receptor/EGFR Ab-10, Mouse Monoclonal Antibody (Thermo Scientific™)

Ensure accurate, reproducible results in immunohistochemistry, western blotting and immunoprecipitation experiments.

Lab Vision™ Vascular Endothelial Growth Factor (VEGF) Ab-3, Mouse Monoclonal Antibody (Thermo Scientific™)

Provide accurate, reproducible results with the Thermo Scientific™ Vascular Endothelial Growth Factor (VEGF) Ab-3, Mouse Monoclonal Antibody.

Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Gibco™)

Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors and is free of viruses, including lactose dehydrogenase elevating virus (LDEV), sometimes found in this type of preparation. The major components of Geltrex™ matrix include laminin, collagen IV, entactin, and heparin sulfate proteoglycans. It is ideal for the promotion and maintenance of differentiated phenotypes in a variety of cell cultures including primary epithelial cells, endothelial cells, smooth muscle cells, and human induced pluripotent stem cells (iPSC). Geltrex™ matrix offers, on average, a 2-fold reduction in several growth factors. As a result, analysis of specific growth factor effects in applications requiring basement membrane extract can be carried out in a controlled and reproducible format.

Key Features of Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix:

• Free of lactose dehydrogenase elevating virus (LDEV) making it ideal for all types of cell culture and mouse in vivo applications
• Consistent protein concentration lot-to-lot helps reduce the need to screen lots
• Protocols for various applications, including tube formation assays, are available

Gain Reliability and Consistency in Your hESC Studies
Recent advances in processing EHS tumor cells have resulted in virus free basement membrane extracts. Every lot is tested by RT-PCR methods so you can now have confidence that every lot of Geltrex™ is virus free. Additionally, our consistent manufacturing process helps ensure a protein concentration with very little variation from lot-to-lot, averaging 15 mg/ml so you can have confidence in every purchase.

Investigate Angiogenesis with Validated Gibco® Protocols
There are many applications for Geltrex™ LDEV-Free reduced growth factor basement membrane matrix, each requiring different thicknesses and concentrations. In general, a protein concentration of >5 mg/ml is used for differentiation studies of primary cells. Geltrex™ LDEV-Free reduced growth factor basement membrane matrix has been used to develop tube formation assay protocols using Gibco® primary cells and specialty media. See this and other angiogenesis assay protocols

Lab Vision™ Epidermal Growth Factor Receptor/EGFR Ab-13, Mouse Monoclonal Antibody (Thermo Scientific™)

Obtain accurate, reproducible results in immunoprecipitation experiments with Thermo Scientific™ Epidermal Growth Factor Receptor/EGFR Ab-13, Mouse Monoclonal Antibody.

Lab Vision™ Epidermal Growth Factor Receptor/EGFR Ab-11, Mouse Monoclonal Antibody (Thermo Scientific™)

Obtain accurate, reproducible results in western blotting and immunoprecipitation experiments.