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Human VEGF165 Animal Free Recombinant Protein, eBioscience™ (Invitrogen™)

Human VEGF-165 Animal Free Recombinant Protein for Ctrl, FN

DKK1 Recombinant Human Protein (Gibco™)

Recombinant Human Dickkopf Homolog 1 (DKK1) is a bioactive protein intended for use in cell culture applications. DKK1 is a member of the dickkopf family. It is a secreted protein with two cysteine-rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway.

High purity—no interference from other proteins or contaminants
High biological activity—more results with less protein
Proven compatibility—Gibco® proteins bioassayed with Gibco® media

High Purity Recombinant Bioactive Protein
Recombinant Human Dickkopf Homolog 1 is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High Biological Activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant Human Dickkopf Homolog 1 (DKK1) specific activity is determined by a functional ELISA measuring the ability of human DKK1 to bind immobilized recombinant human LRP6/Fc chimera receptor.

Proven Compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

IL3 Recombinant Mouse Protein (Gibco™)

Recombinant Mouse Interleukin 3 (IL3) is a bioactive protein intended for use in cell culture applications. IL3 provides the cytokine connection between the immune system and the hematopoietic system. For example, IL3 supports the proliferation and development of almost all types of hematopoietic progenitor cells and can act as a chemoattractant for eosinophils.

High purity—no interference from other proteins or contaminants
High biological activity—more results with less protein
Proven compatibility—Gibco® proteins bioassayed with Gibco® media

High Purity Recombinant Bioactive Protein
Recombinant Mouse Interleukin 3 is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High Biological Activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant Mouse Interleukin 3 (IL3) specific activity is determined by the dose dependent proliferation of NFS-60 cells.

Proven Compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Mouse IL-15/IL-15R Complex Carrier-Free Recombinant Protein, eBioscience™ (Invitrogen™)

Mouse IL-15/IL-15R Complex Carrier-Free Recombinant Protein for Ctrl, FN

Human GM-CSF Carrier-Free Recombinant Protein, eBioscience™ (Invitrogen™)

Human GM-CSF Carrier-Free Recombinant Protein for Ctrl, FN

NGF Recombinant Mouse Protein

Nerve growth factor (NGF), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse NGF (NP_001106168.1), residues 122 to 241, with an initial Met at the N-terminus.

Activity: Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50 for this effec is 0.2-2.0 ng/ml.

Formulation: Lyophilized in 50 mM Na acetate buffer, pH 5.5, 150 mM NaCl, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

IL7 Recombinant Human Protein (Gibco™)

Recombinant Human Interleukin 7 (IL7) is a bioactive protein intended for use in cell culture applications. IL7 stimulates the proliferation of pre-B and pro-B cells. IL7 also supports the maturation of megakaryocytes.

High purity—no interference from other proteins or contaminants
High biological activity—more results with less protein
Proven compatibility—Gibco® proteins bioassayed with Gibco® media

High Purity Recombinant Bioactive Protein
Recombinant Human Interleukin 7 is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High Biological Activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant Human Interleukin 7 (IL7) specific activity is determined by the dose dependent proliferation of human PBMCs.

Proven Compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

HGF Recombinant Human Protein (Gibco™)

Recombinant Human Hepatocyte Growth Factor (HGF) is a bioactive protein intended for use in cell culture applications. HGF plays a role in positive regulation of cell proliferation.

High purity—no interference from other proteins or contaminants
High biological activity—more results with less protein
Proven compatibility—Gibco® proteins bioassayed with Gibco® media

High Purity Recombinant Bioactive Protein
Recombinant Human Hepatocyte Growth Factor is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High Biological Activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant Human Hepatocyte Growth Factor (HGF) specific activity is determined by the dose dependent proliferation of monkey 4MBr-5 cells.

Proven Compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Human FGF basic Animal Free Recombinant Protein, eBioscience™ (Invitrogen™)

Human FGF basic Animal Free Recombinant Protein for Ctrl, FN

IL17 Recombinant Human Protein (Gibco™)

Recombinant IL17 is a bioactive protein intended for use in cell culture applications. IL17 stimulates epithelial, endothelial or fibroblastic cells to secrete IL-6, IL-8 and G-CSF. IL17 also increases the expression of ICAM-1 in fibroblasts.

NGF Recombinant Human Protein

Nerve growth factor (NGF) recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of human NGF (NP_002497.2) (Ser 122-Arg 239).

N-terminal Sequence Analysis: Ser 122

Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is typically 0.4-1.0 ng/mL.

Formulation: Lyophilized in 50 mM NaAc, 150 mM NaCl, pH 5.5, 5% mannitol, 5% trehalose, 0.02% Tween®-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

Background
As the first one of a series of neurotrophic factors, nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF, was demonstrated to exhibits the growth stimulating activity of NGF. β-NGF is a target-derived secreted homodimeric protein of two 120-amino acid mature polypeptides after proteolytic removal of the signal and pro peptide. NGF acts via at least two receptors on the surface of cells, TrkA and p75 receptors to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In additon to its neurotropic activities, studies indicated that NGF may also have an important role in the regulation of the immune system. The synthesis of β-NGF in astrocytes is enhanced by various other cytokines including IL-1, TNF-α, PDGF and TGF-β .Defects in β-NGF are the cause of hereditary sensory and autonomic neuropathy type 5 (HSAN5) characterized by degeneration of dorsal root and autonomic ganglion cells, as well as sensory and/or autonomic abnormalities.

You may also be interested in the following NGF products:
Enzyme & Protein Assays
Primary Antibodies
Gene Expression Assays

FLT3 Ligand Recombinant Human Protein (Gibco™)

Recombinant FLT3L is a bioactive protein intended for use in cell culture applications. FLT3L (fms-like tyrosine kinase-3 ligand) is a cytokine involved in hematopoeisis.

Human IL-1 alpha Carrier-Free Recombinant Protein, eBioscience™ (Invitrogen™)

Human IL-1 alpha Carrier-Free Recombinant Protein for Ctrl, FN

NGF Mouse Protein, Native, 7S Subunit (Gibco™)

Natural Mouse Nerve Growth Factor 7S (NGF 7S) is a bioactive protein intended for use in cell culture applications. NGF 7S, Murine, Natural, is a 130- to 140-kDa complex that regulates the development and maintenance of sympathetic and some sensory neurons NGF is used in studies of nerve cell growth and differentiation. It can also be used as a medium supplement to support the growth or survival of neuronal cells in culture.

High purity—no interference from other proteins or contaminants
High biological activity—more results with less protein
Proven compatibility—Gibco® proteins bioassayed with Gibco® media

High Purity Recombinant Bioactive Protein
Natural Mouse Nerve Growth Factor 7S is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High Biological Activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Natural Mouse Nerve Growth Factor 7S (NGF 7S) specific activity is determined by measuring neurite outgrowth in chick embryo dorsal root ganglia.

Proven Compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

BD1 Recombinant Human Protein (Gibco™)

Recombinant β-defensin 1 (BD1) is a bioactive recombinant protein intended for use in cell culture applications. BD1 is a member of the defensin family involved in defense against mibcrobes.