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Cytokine/Chemokine/Growth Factor Convenience 45-Plex Human ProcartaPlex™ Panel 1 Invitrogen™

The Human Cytokine/Chemokine/Growth Factor Convenience 45-Plex ProcartaPlex Panel 1 enables the study of immune function by analyzing 45 protein targets in a single well using Luminex xMAP technology. The panel is provided in a ready-to-use format with individual vials of capture and detection reagents formulated at 1X concentration, requiring less pipetting and experimental setup. The same targets can be found in the Human Cytokine/Chemokine/Growth Factor 45-Plex ProcartaPlex Panel 1 (Cat. No. EPX450-12171-901) that provides a modular format allowing for more flexibility to increase or decrease the number of targets.

ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit our ProcartaPlex Immunoassays page for more information and available products.

Target list [bead region]:
Th1/Th2: GM-CSF [44], IFN gamma [43], IL-1 beta [18], IL-2 [19], IL-4 [20], IL-5 [21], IL-6 [25], IL-12p70 [34], IL-13 [35], IL-18 [66], TNF alpha [45]
Th9/Th17/Th22/Treg: IL-9 [52], IL-10 [28], IL-17A (CTLA-8) [36], IL-21 [72], IL-22 [76], IL-23 [63], IL-27 [14]
Inflammatory cytokines: IFN alpha [48], IL-1 alpha [62], IL-1RA [38], IL-7 [26], IL-15 [65], IL-31 [37], TNF beta [54]
Chemokines: Eotaxin (CCL11) [33], GRO alpha (CXCL1) [61], IP-10 (CXCL10) [22], MCP-1 (CCL2) [51], MIP-1 alpha (CCL3) [12], MIP-1 beta (CCL4) [47], RANTES (CCL5) [42], SDF-1 alpha [13]
Growth factors: BDNF [57], EGF [56], FGF-2 [75], HGF [46], NGF beta [55], PDGF-BB [77], PlGF-1 [29], SCF [39], VEGF-A [78], VEGF-D [53]

About ProcartaPlex assays for the Luminex platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Features include:• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
• Well-established Luminex technology highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Lab Vision™ Epidermal Growth Factor Receptor (EGFR), Rabbit Monoclonal Antibody Thermo Scientific™

Ensure accurate, reproducible results in immunohistochemistry experiments with Thermo Scientific™ EGFR, Rabbit Monoclonal Antibody, part of a comprehensive antibody portfolio. Studies show rabbit monoclonal antibodies are more sensitive and specific than other available markers.

Lab Vision™ Epidermal Growth Factor Receptor/EGFR Ab-2, Mouse Monoclonal Antibody Thermo Scientific™

Ensure accurate, reproducible results in immunoprecipitation experiments with Thermo Scientific™ Epidermal Growth Factor Receptor/EGFR Ab-2, Mouse Monoclonal Antibody.

Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 488 Streptavidin (Alexa Fluor™ 488 EGF complex) Invitrogen™

We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green® 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor® dyes and Texas Red® dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor.

Epidermal Growth Factor Conjugate Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Biotin-XX complexed with Alexa Fluor® 488 streptavidin (~495/519 nm)
• Number of fluorophore molecules on each EGF molecule: ~2–3
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter


Find More Receptor Binding and Phagocytosis Probes
We offer a number of fluorescently labeled probes for studying receptor-mediated endocytosis, membrane markers for endocytosis and exocytosis, and methods for detecting internalized fluorescent ligands. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

Lab Vision™ Transforming Growth Factor α (TGFα) Ab-1, Mouse Monoclonal Antibody Thermo Scientific™

Provide accurate, reproducible results with the Thermo Scientific™ Transforming Growth Factor α (TGFα) Ab-1, Mouse Monoclonal Antibody.

Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 555 Streptavidin (Alexa Fluor™ 555 EGF complex) Invitrogen™

We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green® 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor® dyes and Texas Red® dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor.

Epidermal Growth Factor Conjugate Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Biotin-XX complexed with Alexa Fluor® 555 streptavidin (~555/565 nm)
• Number of fluorophore molecules on each EGF molecule: ~2–3
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter


Find More Receptor Binding and Phagocytosis Probes
We offer a number of fluorescently labeled probes for studying receptor-mediated endocytosis, membrane markers for endocytosis and exocytosis, and methods for detecting internalized fluorescent ligands. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

Hepatocyte Growth Factor Ab-2, Mouse Monoclonal Antibody Thermo Scientific™

Ideal for Western Blotting, Thermo Scientific™ Hepatocyte Growth Factor Ab-2, Mouse Monoclonal Antibody provides accurate, reproducible results.

Epidermal Growth Factor, Biotin-XX Conjugate (biotin EGF) Invitrogen™

This purified epidermal growth factor (EGF) contains a single biotin-XX molecule on the N-terminal amino acid. Biotin-XX EGF contains a long spacer arm that enhances the probe’s affinity for the EGF receptor and facilitates binding of a label such as a dye–streptavidin molecule, a Qdot® nanocrystals–streptavidin molecule, or an enzyme-conjugated streptavidin. Labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more.

Epidermal Growth Factor Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Number of biotin-XX molecules on each EGF molecule: 1


Find Fluorescent EGF Conjugates
In addition to biotinylated EGF, we offer biotinylated EGF precomplexed to selected fluorescent streptavidin labels and EGF directly labeled with some fluorescent dyes. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Lab Vision™ Epidermal Growth Factor Receptor/EGFR Ab-10, Mouse Monoclonal Antibody Thermo Scientific™

Ensure accurate, reproducible results in immunohistochemistry, western blotting and immunoprecipitation experiments with Thermo Scientific™ Epidermal Growth Factor Receptor/EGFR Ab-10, Mouse Monoclonal Antibody.

Hepatocyte Growth Factor Ab-1, Mouse Monoclonal Antibody Thermo Scientific™

Ensure accurate, reproducible results with Thermo Scientific™ Hepatocyte Growth Factor Ab-1, Mouse Monoclonal Antibody.

Lab Vision™ Epidermal Growth Factor Receptor/EGFR Ab-15, Mouse Monoclonal Antibody Thermo Scientific™

Ensure accurate, reproducible results in western blotting and immunoprecipitation experiments with Thermo Scientific™ Epidermal Growth Factor Receptor/EGFR Ab-15, Mouse Monoclonal Antibody.

Lab Vision™ Vascular Endothelial Growth Factor (VEGF) Ab-3, Mouse Monoclonal Antibody Thermo Scientific™

Provide accurate, reproducible results with the Thermo Scientific™ Vascular Endothelial Growth Factor (VEGF) Ab-3, Mouse Monoclonal Antibody.

Lab Vision™ Epidermal Growth Factor Receptor/EGFR Ab-5, Mouse Monoclonal Antibody Thermo Scientific™

Obtain accurate, reproducible results in immunohistochemistry, western blotting, immunofluorescence, immunoprecipitation and flow cytometry experiments with Thermo Scientific™ Epidermal Growth Factor Receptor/EGFR Ab-5, Mouse Monoclonal Antibody.

Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 647 Streptavidin (Alexa Fluor™ 647 EGF complex) Invitrogen™

We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green® 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor® dyes and Texas Red® dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor.

Epidermal Growth Factor Conjugate Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Biotin-XX complexed with Alexa Fluor® 647 streptavidin (~650/665 nm)
• Number of fluorophore molecules on each EGF molecule: ~2–3
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter


Find More Receptor Binding and Phagocytosis Probes
We offer a number of fluorescently labeled probes for studying receptor-mediated endocytosis, membrane markers for endocytosis and exocytosis, and methods for detecting internalized fluorescent ligands. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

Epidermal Growth Factor, Fluorescein Conjugate (Fluorescein EGF) Invitrogen™

We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green® 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor® dyes and Texas Red® dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor.

Epidermal Growth Factor Conjugate Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Fluorescein (~494/518 nm)
• Number of fluorophore molecules on each EGF molecule: 1
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter


Find More Receptor Binding and Phagocytosis Probes
We offer a number of fluorescently labeled probes for studying receptor-mediated endocytosis, membrane markers for endocytosis and exocytosis, and methods for detecting internalized fluorescent ligands. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

Lab Vision™ Vascular Endothelial Growth Factor (VEGF) Ab-7, Mouse Monoclonal Antibody Thermo Scientific™

Provide accurate, reproducible results with the Thermo Scientific™ Vascular Endothelial Growth Factor (VEGF) Ab-7, Mouse Monoclonal Antibody.

Lab Vision™ Epidermal Growth Factor Receptor/EGFR Ab-11, Mouse Monoclonal Antibody Thermo Scientific™

Obtain accurate, reproducible results in western blotting and immunoprecipitation experiments with Thermo Scientific™ Epidermal Growth Factor Receptor/EGFR Ab-11, Mouse Monoclonal Antibody.

Growth Factor 7-Plex NHP ProcartaPlex™ Panel Invitrogen™

The Non-Human Primate Growth Factor 7-Plex ProcartaPlex Panel enables the exploration of the immune response by analyzing 7 protein targets in a single well using Luminex xMAP technology. This panel is a module of the Non-Human Primate Cytokine/Chemokine/Growth Factor 37-Plex ProcartaPlex Panel (Cat. No. EPX370-40045-901). This kit is fully combinable with the other modules or individual simplex assays corresponding to the targets in the 37-plex panel.

ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit our ProcartaPlex Immunoassays page for more information and available products.

Target list [bead region]: BDNF [57], FGF-2 [75], NGF beta [55], PDGF-BB [77], SCF [39], VEGF-A [78], VEGF-D [53]

About ProcartaPlex assays for the Luminex platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Features include:• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
• Well-established Luminex technology highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Epidermal Growth Factor, Biotinylated, complexed to Texas Red™ Streptavidin (Texas Red™ EGF complex) Invitrogen™

We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green® 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor® dyes and Texas Red® dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor.

Epidermal Growth Factor Conjugate Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Biotin-XX complexed with Texas Red® streptavidin (~595/615 nm)
• Number of fluorophore molecules on each EGF molecule: ~2–3
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter


Find More Receptor Binding and Phagocytosis Probes
We offer a number of fluorescently labeled probes for studying receptor-mediated endocytosis, membrane markers for endocytosis and exocytosis, and methods for detecting internalized fluorescent ligands. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

Lab Vision™ Vascular Endothelial Growth Factor (VEGF), Rabbit Polyclonal Antibody Thermo Scientific™

Take advantage of one marker in a comprehensive antibody portfolio that addresses all major disease states with the Thermo Scientific™ Vascular Endothelial Growth Factor (VEGF), Rabbit Polyclonal Antibody.

Lab Vision™ Epidermal Growth Factor Receptor/EGFR Ab-13, Mouse Monoclonal Antibody Thermo Scientific™

Obtain accurate, reproducible results in immunoprecipitation experiments with Thermo Scientific™ Epidermal Growth Factor Receptor/EGFR Ab-13, Mouse Monoclonal Antibody.

pHrodo™ Green Epidermal Growth Factor (EGF) Conjugate Invitrogen™

pHrodo Green Epidermal Growth Factor (EGF) Conjugate is used for the detection of internalized EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. Fluorescent labels that respond to a pH change add another level of detection. pHrodo Green is weakly fluorescent outside of cells at neutral pH, but when internalized specifically through EGF endocytosis, it becomes brightly fluorescent in the acidic endosomes. This allows the measurement of internalized EGF, as well as the dynamic monitoring of EGF internalization in live cells.

Features of this EGF conjugate include:

• pHrodo Green dye conjugated directly to highly active human recombinant EGF molecule
• Excitation/emission of 509/533 nm
• Excellent photostability
• Appropriate for multiplexing with red and deep red fluorophores such as RFP, Alexa Fluor 594, MitoTracker Red, and MitoTracker Deep Red

pHrodo Green Epidermal Growth Factor (EGF) Conjugate is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometery, and microplate-based fluorimetry or high throughput screening (HTS). This reagent is also compatible with various benchtop instruments such as the Evos, FLoid, Tali, and Attune instruments.

Geltrex™ LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix Gibco™

Geltrex™ LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors and is free of viruses, including lactose dehydrogenase elevating virus (LDEV), sometimes found in this type of preparation. Routinely used for attachment and maintenance of human embryonic stem cells (hESC), every lot of Geltrex™ LDEV-free hESC-qualified Reduced Growth Factor Basement Membrane Matrix is quality control tested using hESCs and StemPro hESC Serum Free Media to help ensure growth and pluripotency of each lot.

Key Features of Geltrex™ LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix:

• Every lot is qualified using hESC, which helps reduce the need for customers to lot screen
• Free of the lactose dehydrogenase elevating virus (LDEV) making it safe for all types of cell culture
• Consistent protein concentration lot-to-lot helps ensure reproducible results

Gain Reliability and Consistency in Your hESC Studies
Human ESCs have specific needs for attachment and maintenance and some lots of basement membrane extract may not provide adequate support. Geltrex™ hESC-qualified reduced growth factor basement membrane matrix has been tested for support of hESC growth and pluripotency, therefore minimizing the need for you to test multiple lots to find one that works. Our consistent manufacturing process helps ensure a protein concentration averaging 15 mg/ml with very little variation from lot-to-lot, so you can have confidence in every purchase.

Free of LDEV Contamination
Recent advances in processing EHS tumor cells have resulted in virus free basement membrane extracts. Every lot is tested by tested by RT-PCR methods so you can now have confidence that every lot of Geltrex™ is virus-free.

Cytokine/Chemokine/Growth Factor 11-Plex Canine ProcartaPlex™ Panel 1 Invitrogen™

The Canine Cytokine/Chemokine/Growth Factor 11-Plex ProcartaPlex Panel enables the study of immune function by analyzing 11 protein targets in a single well using Luminex xMAP technology. Analyze 11 protein targets simultaneously for efficient immune response profiling, biomarker discovery, and validation.

ProcartaPlex Preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit our ProcartaPlex Immunoassays page for more information and available products.

Target list [bead region]: IFN gamma [43], IL-2 [19], IL-6 [25], IL-8 (CXCL8) [27], IL-10 [28], IL-12/IL-23p40 [33], MCP-1 (CCL2) [51], NGF beta [38], SCF [78], TNF alpha [45], VEGF-A [56]

About ProcartaPlex assays for the Luminex platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Features include:• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
• Well-established Luminex technology highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Epidermal Growth Factor, Tetramethylrhodamine Conjugate (rhodamine EGF) Invitrogen™

We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green® 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor® dyes and Texas Red® dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor.

Epidermal Growth Factor Conjugate Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Tetramethylrhodamine (~555/580 nm)
• Number of fluorophore molecules on each EGF molecule: 1
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter


Find More Receptor Binding and Phagocytosis Probes
We offer a number of fluorescently labeled probes for studying receptor-mediated endocytosis, membrane markers for endocytosis and exocytosis, and methods for detecting internalized fluorescent ligands. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix Gibco™

Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors. This pre-diluted version of the matrix can be taken directly from the refrigerator and dispensed into cell culture plates without further handling. Geltrex hESC-Qualified Ready-To-Use basement membrane matrix is optimized for stem cell culture applications using StemPro hESC SFM. For stem cell culture using the StemFlex Medium or Essential 8 Media System, use Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix.

Geltrex hESC-Qualified Ready-To-Use matrix is:

• Consistent—pre-dilution at a concentration of 1:100 ensures optimal protein concentration for stem cell attachment to standard cell culture plates
• Stable—storage at 4°C eliminates overnight thawing on ice
• Cost effective— the ready-to-use format saves multiple preparation steps and potential for product failure

Reduced variability
Geltrex hESC-Qualified Ready-To-Use matrix is provided as a 1:100 dilution in a buffered solution that can be added directly to cell culture plates for coating. Each lot is prepared at the appropriate protein concentration for optimal stem cell attachment, which minimizes the need to calculate dilution schemes for every lot of matrix.

Reliable
Designed for 4°C storage for the life of the product, this matrix can be kept in the refrigerator along with your cell culture media—no more overnight thawing in clumsy ice baths and no risk of premature gelling. In addition, every lot is functionally qualified using human embryonic stem cells and tested to ensure it is free of lactose dehydrogenase elevating virus (LDEV), making it safe for all cell culture labs.

Cost effective
Geltrex hESC-Qualified Ready-To-Use matrix eliminates multiple steps—no more freezing, thawing, diluting, and aliquoting. This product is a true time saver.

Growth Factor 11-Plex Human ProcartaPlex™ Panel Invitrogen™

The Human Growth Factor 11-Plex Human ProcartaPlex Panel enables researchers to explore the immune response by analyzing 11 protein targets in a single well using Luminex xMAP technology. This panel is a module of the Cytokine/Chemokine/Growth Factor 45-Plex ProcartaPlex Panel 1 (Cat. No. EPX450-12171-901). This kit is fully combinable with the other modules or individual simplex assays corresponding to the targets in the 45-plex panel.

ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit our ProcartaPlex Immunoassays page for more information and available products.

Target list [bead region]: BDNF [57], EGF [56], FGF-2 [75], HGF [46], LIF [15], NGF beta [55], PDGF-BB [77], PlGF-1 [29], SCF [39], VEGF-A [78], VEGF-D [53]

About ProcartaPlex assays for the Luminex platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Features include:• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
• Well-established Luminex technology highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Cytokine/Chemokine/Growth Factor 45-Plex Human ProcartaPlex™ Panel 1 Invitrogen™

The Human Cytokine/Chemokine/Growth Factor 45-Plex ProcartaPlex Panel 1 enables the exploration of immune function by analyzing 45 protein targets in a single well using Luminex xMAP technology. The panel is comprised of five modular sub-panels that allow for an initial broad screen to determine targets of interest and subsequent studies analyzing specific functional subsets of cytokines that can be purchased separately. The sub-panels are fully combinable with each other or with individual simplex assays corresponding to the targets in the panel. The same targets can be found in the Cytokine/Chemokine/Growth Factor Convenience 45-Plex ProcartaPlex Panel 1 (Cat. No. EPXR450-12171-901) that is in a ready-to-use format that requires less pipetting.

ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit our ProcartaPlex Immunoassays page for more information and available products.

Target list [bead region]:
Th1/Th2: GM-CSF [44], IFN gamma [43], IL-1 beta [18], IL-2 [19], IL-4 [20], IL-5 [21], IL-6 [25], IL-12p70 [34], IL-13 [35], IL-18 [66], TNF alpha [45]
Th9/Th17/Th22/Treg: IL-9 [52], IL-10 [28], IL-17A (CTLA-8) [36], IL-21 [72], IL-22 [76], IL-23 [63], IL-27 [14]
Inflammatory cytokines: IFN alpha [48], IL-1 alpha [62], IL-1RA [38], IL-7 [26], IL-15 [65], IL-31 [37], TNF beta [54]
Chemokines. Eotaxin (CCL11) [33], GRO alpha (CXCL1) [61], IP-10 (CXCL10) [22], MCP-1 (CCL2) [51], MIP-1 alpha (CCL3) [12], MIP-1 beta (CCL4) [47], RANTES (CCL5) [42], SDF-1 alpha [13]
Growth factors: BDNF [57], EGF [56], FGF-2 [75], HGF [46], NGF beta [55], PDGF-BB [77], PlGF-1 [29], SCF [39], VEGF-A [78], VEGF-D [53]

About ProcartaPlex assays for the Luminex platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Features include:• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
• Well-established Luminex technology highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

pHrodo™ Red Epidermal Growth Factor (EGF) Conjugate Invitrogen™

pHrodo Red Epidermal Growth Factor (EGF) Conjugate is used for the detection of internalized EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. Fluorescent labels that respond to a pH change add another level of detection. pHrodo Red is weakly fluorescent outside of cells at neutral pH, but when internalized specifically through EGF endocytosis, it becomes brightly fluorescent in the acidic endosomes. This allows the measurement of internalized EGF, as well as the dynamic monitoring of EGF internalization in live cells.

Features of this EGF conjugate include:

• pHrodo Red dye conjugated directly to highly active human recombinant EGF molecule
• Excitation/emission of 560/585 nm
• Excellent photostability
• Appropriate for multiplexing with green fluorophores such as GFP, FITC, and Alexa Fluor 488

pHrodo Red Epidermal Growth Factor (EGF) Conjugate is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometery, and microplate-based fluorimetry or high throughput screening (HTS). This reagent is also compatible with various benchtop instruments such as the Evos, FLoid, Tali, and Attune instruments.

Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix Gibco™

Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors and is free of viruses, including lactose dehydrogenase elevating virus (LDEV), sometimes found in this type of preparation. The major components of Geltrex™ matrix include laminin, collagen IV, entactin, and heparin sulfate proteoglycans. It is ideal for the promotion and maintenance of differentiated phenotypes in a variety of cell cultures including primary epithelial cells, endothelial cells, smooth muscle cells, and human induced pluripotent stem cells (iPSC). Geltrex™ matrix offers, on average, a 2-fold reduction in several growth factors. As a result, analysis of specific growth factor effects in applications requiring basement membrane extract can be carried out in a controlled and reproducible format.

Key Features of Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix:

• Free of lactose dehydrogenase elevating virus (LDEV) making it ideal for all types of cell culture and mouse in vivo applications
• Consistent protein concentration lot-to-lot helps reduce the need to screen lots
• Protocols for various applications, including tube formation assays, are available

Gain Reliability and Consistency in Your hESC Studies
Recent advances in processing EHS tumor cells have resulted in virus free basement membrane extracts. Every lot is tested by RT-PCR methods so you can now have confidence that every lot of Geltrex™ is virus free. Additionally, our consistent manufacturing process helps ensure a protein concentration with very little variation from lot-to-lot, averaging 15 mg/ml so you can have confidence in every purchase.

Investigate Angiogenesis with Validated Gibco® Protocols
There are many applications for Geltrex™ LDEV-Free reduced growth factor basement membrane matrix, each requiring different thicknesses and concentrations. In general, a protein concentration of >5 mg/ml is used for differentiation studies of primary cells. Geltrex™ LDEV-Free reduced growth factor basement membrane matrix has been used to develop tube formation assay protocols using Gibco® primary cells and specialty media. See this and other angiogenesis assay protocols
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