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Regulatory statements vary by product. Please see product-specific literature to determine product use.

Can I use Lipofectamine MessengerMAX Transfection Reagent to deliver plasmid DNA? Product FAQ

Answer

Yes, Lipofectamine MessengerMAX Transfection Reagent can deliver plasmid DNA in combination with mRNA (i.e., in CRISPR); however, Lipofectamine 3000 Transfection Reagent is best optimized for plasmid DNA delivery and superior DNA transfection performance.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9032

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Which transfection reagent should I use for delivering my gene of interest into stem cells? Product FAQ

Answer

We recommend using Lipofectamine Stem Transfection Reagent for delivery of DNA, mRNA and co-transfection (siRNA and plasmid DNA) in a wide variety of stem cells. Lipofectamine Stem Transfection Reagent also delivers Cas9-gRNA ribonucleoproteins for gene editing applications. For more information on the transfection efficiency and versatility of the reagent, please visit: https://www.thermofisher.com/us/en/home/brands/product-brand/lipofectamine/lipofectamine-stem-transfection-reagent.html

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9010

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The shipping was delayed for my Lipofectamine 3000 Transfection Reagent. Is it still good for use? Product FAQ

Answer

Yes, Lipofectamine 3000 Transfection Reagent is stable at room temperature for weeks to months.

Answer Id: E21065

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Is the Lipofectamine 3000 Transfection Reagent animal origin-free? Product FAQ

Answer

Yes, Lipofectamine 3000 Transfection Reagent is an animal origin-free (AOF) product.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9012

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What transfection methods do you recommend when working with your CRISPR products? Product FAQ

Answer

For transfecting of the Cas9 protein, we would recommend using the Neon transfection system or Lipofectamine CRISPRMAX Cas9 Transfection Reagent. For transfection of mRNA, we would recommend using Lipofectamine MessengerMAX Transfection Reagent. For transfection of CRISPR vectors, we would recommend using Lipofectamine 3000 Transfection reagent.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E11535

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What are the recommended culture media volumes for transfection with Lipofectamine 3000 Transfection Reagent? Product FAQ

Answer

We do not have specific culture media volume recommendations for transfection with Lipofectamine 3000 Transfection Reagent, but the following link outlines standard culture media volumes for different plate formats: https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/cell-culture-protocols/cell-culture-useful-numbers.html
The ranges in this reference table can be used to optimize your own transfections.

Answer Id: E21085

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I accidentally left my Lipofectamine 3000 Transfection Reagent at room temperature. Can I still use it? Product FAQ

Answer

Yes, Lipofectamine 3000 Transfection Reagent is stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

Answer Id: E9068

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Can I purchase the P3000 Enhancer Reagent as a stand-alone product? Product FAQ

Answer

Sorry, the P3000 Enhancer Reagent is only available bundled along with Lipofectamine 3000 Transfection Reagent.

Depending on your optimization ratios between Lipofectamine 3000 Transfection Reagent and P3000 Enhancer Reagent, it is possible that one of the reagents will run out sooner than the other. We purposely designed the formulation such that regardless of your optimization ratio, you will receive a high number of reactions because of the superior efficiency of this product.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E18953

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Can I use Lipofectamine 3000 for transfection of suspension cells, other hard-to-transfect cells, and primary cells? Product FAQ

Answer

You can use Lipofectamine 3000 for transfection of suspension cells, hard-to-transfect cells, and primary cells. Please see data here (https://www.thermofisher.com/us/en/home/brands/product-brand/lipofectamine/lipofectamine-3000.html). However, if you experience low transfection efficiency, we recommend the Neon Transfection System or the ViraPower Lentiviral Delivery Systems.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9013

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Why should I pick Lipofectamine 3000 Transfection Reagent over other lipid reagents you offer? Product FAQ

Answer

Lipofectamine 3000 Transfection Reagent was specifically designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cells. It works well for plasmid DNA transfection, siRNA transfection, and co-transfection of plasmid DNA with siRNA.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9011

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What is the difference between Lipofectamine Stem Transfection Reagent and Lipofectamine 3000 Transfection Reagent? Product FAQ

Answer

Lipofectamine Stem Transfection Reagent is a lipid-based transfection reagent that is specifically designed for stem cells. It is our most versatile, and preferred choice for delivering DNA, mRNA, and Cas9 protein (RNP) into human pluripotent stem cells, as well as neural stem cells (NSCs) and mesenchymal stem cells (MSCs). Lipofectamine 3000 Transfection Reagent can be used to deliver DNA or RNA and is recommended for hard-to-transfect cell lines and workhorse cell lines.

Answer Id: E15200

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How do I use Lipofectamine 3000 Transfection Reagent for co-transfection of plasmids? Product FAQ

Answer

Just keep the total DNA amount the same (e.g., half of the amount for each plasmid, or any other suitable ratio). Everything else remains the same.

Answer Id: E21187

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Can Lipofectamine 3000 be used for transfecting primary cells? Product FAQ

Answer

Yes, Lipofectamine 3000 can be used to transfect primary cells including neurons, stem cells, and blood cells as shown here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/transfection-reagent-application-table.html). For very difficult-to-transfect cells, we recommend the Neon Transfection System.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9014

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Can I use Lipofectamine LTX for transfection of suspension cells, other hard-to-transfect cells, and primary cells? Product FAQ

Answer

We recommend using Lipofectamine 3000 which demonstrates much higher transfection efficiency and protein expression over all other transfection reagents for suspension, hard-to-transfect, and primary cells. Review Lipofectamine 3000 data for over 60 cell lines here https://www.thermofisher.com/us/en/home/brands/product-brand/lipofectamine/lipofectamine-3000.html. For highest transfection efficiencies, we recommend the Neon Transfection System for Jurkat (94%) and K562 (90%) cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9024

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Which transfection reagent do you recommend for RNAi applications? Product FAQ

Answer

We recommend using Lipofectamine RNAiMAX Reagent for delivery of siRNA and miRNA into all cell types. It has been specifically developed for siRNA transfection while providing high transfection efficiency with minimal cytotoxicity. As a result, less optimization is necessary. For vector DNA-based RNAi applications, we recommend Lipofectamine 3000 Reagent with the P3000 Enhancer Reagent. For CRISPR-mediated gene knockout methods, we recommend Lipofectamine CRISPRMAX for Cas9/gRNA ribonucleoprotein, Lipofectamine MessengerMAX for Cas9 mRNA, and Lipofectamine 3000 with the P3000 Enhancer Reagent for CRISPR plasmid DNA-based delivery. For all difficult-to-transfect cells, we recommend using electroporation methods such as the Neon Transfection System.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9000

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