Documents & Support

1-15 of 64 Results

Regulatory statements vary by product. Please see product-specific literature to determine product use.

How do I choose between Lipofectamine 2000 and Lipofectamine LTX? Product FAQ

Answer

Lipofectamine LTX and Lipofectamine 2000 are both recommended for plasmid DNA transfections. However, if you notice increased cytotoxicity with Lipofectamine 2000, Lipofectamine LTX is the preferred reagent due to significantly reduced cytotoxicity levels and improved transfection performance in a number of hard-to-transfect cell lines.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E4997

Was this answer helpful?

Yes No

Thank you for your response

Can I use Lipofectamine LTX for delivery of plasmid DNA and siRNA? Product FAQ

Answer

Lipofectamine LTX was designed for delivery of plasmid DNA with minimal cytotoxicity. We do not recommend using it for siRNA delivery. We recommend using Lipofectamine 3000 with the P3000 Enhancer for co-transfection applications.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9022

Was this answer helpful?

Yes No

Thank you for your response

Can Lipofectamine LTX be used in RNAi experiments? Product FAQ

Answer

Lipofectamine LTX performs well with vector-based RNAi experiments since you are transfecting DNA. But for direct transfection of RNA molecules like siRNA and Stealth RNAi negative control transfections, we recommend Lipofectamine RNAiMAX.

Answer Id: E4994

Was this answer helpful?

Yes No

Thank you for your response

Can I use Lipofectamine LTX in RNAi experiments? Product FAQ

Answer

We recommend Lipofectamine 3000 for delivery of vector-based RNAi but for direct transfection of synthetic siRNA, we recommend using Lipofectamine RNAiMAX. For in vivo siRNA experiments, we recommend Invivofectamine 3.0 Reagent.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9025

Was this answer helpful?

Yes No

Thank you for your response

I would like to scale up my transfection with Lipofectamine LTX & PLUS Reagent to 10 cm plates. What protocol should I use? Product FAQ

Answer

For scaling up your transfection with Lipofectamine LTX & PLUS Reagent, please refer to this link (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/LipofectamineLTX_PLUS_Reag_protocol.pdf) and click on "Scaling Up or Down Transfections".

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E18959

Was this answer helpful?

Yes No

Thank you for your response

What is the difference between Lipofectamine and Lipofectamine PLUS reagent? Product FAQ

Answer

Lipofectamine and the PLUS Reagent are utilized for transfection of a wide range of cell types. In the past, Invitrogen sold Lipofectamine with the PLUS reagent. This is no longer the case. Generally speaking, one would not use the PLUS Reagent by itself. It is used in conjunction with some other reagent, such as Lipofectamine, Lipofectamine LTX or in some cases, with Lipofectin. Lipofectamine LTX is offered in combination with the PLUS reagent in catalog numbers A12621 and 15338100. We do not recommend using the PLUS Reagent with Lipofectamine 2000 as it does not generally help.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E3035

Was this answer helpful?

Yes No

Thank you for your response

How does Lipofectamine LTX compare to Lipofectamine 2000? Product FAQ

Answer

Lipofectamine LTX and Lipofectamine 2000 are both recommended for plasmid DNA transfection. However, in a panel of 16 cell lines tested, LTX showed an average of 2.5-fold increase in expression and 25% lower cytotoxicity compared to Lipofectamine 2000.

For RNA applications, Lipofectamine 2000 has well-tested protocols for efficient RNA transfection and co-transfection of RNA and DNA. Lipofectamine LTX is not recommended for use with RNA.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E4998

Was this answer helpful?

Yes No

Thank you for your response

Can Lipofectamine LTX be used for transfecting primary cells? Product FAQ

Answer

We have tested four primary cell lines: HuVec, NHFF (Normal human foreskin fibroblast), MJ90 and NDHF. We have observed transfection efficiency in the range of 5-25%. We suggest optimizing transfection efficiency for your primary cell line by varying DNA and lipid ratio. If you are using any other primary cell line, please follow guidelines suggested in the product insert. Alternatively, Neon electroporation system and viral delivery methods are recommended.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E4996

Was this answer helpful?

Yes No

Thank you for your response

Can Lipofectamine LTX be used in transfection of Suspension cells and other hard to transfect cells? Product FAQ

Answer

We have tested transfection of suspension cells such as Jurkat and K562. We achieved transfection efficiency of less than 5% for each of them. We do not notice toxicity with Lipofectamine LTX reagent when used with these suspension cells. For other cell lines please follow the guidelines in the product insert. However, if you experience low transfection efficiency, then we recommend Neon electroporation system or Viral delivery.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E4995

Was this answer helpful?

Yes No

Thank you for your response

Which lipid transfection reagent do you recommend for my cell line? Product FAQ

Answer

The best transfecting agent and efficiency would depend on the particular cell line you have. Please visit our online selection tools to see our recommendation for your specific cell line. If your cell line is not on the list, we recommend you try Lipofectamine LTX or Lipofectamine 2000 for plasmid transfection, and Lipofectamine RNAiMAX for siRNA transfection. For primary cells, Lipofectamine LTX with PLUS reagent is generally the best choice for plasmid transfection. For some hard-to-transfect cells, like suspension cells and stem cells, the Neon electroporation system usually works better compared to lipid transfection reagent.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E4502

Was this answer helpful?

Yes No

Thank you for your response

Can I use Lipofectamine LTX for transfecting plasmid DNA into primary cells? Product FAQ

Answer

We have tested four primary cell lines: HuVEC (human ubilical vein endothelial cells), NHFF (normal human foreskin fibroblast), MJ90, and NDHF with transfection efficiencies between 5-25%. Instead, we recommend using Lipofectamine 3000 which shows highest transfection efficiency for all cell types and optimize by varying the DNA and lipid ratio. Alternatively, the Neon Transfection System shows 75-80% transfection efficiency. Please visit our Neon cell specific protocol database for more information: https://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/transfection---selection-misc/neon-transfection-system/neon-protocols-cell-line-data.html?icid=fr-neon-3

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9023

Was this answer helpful?

Yes No

Thank you for your response

How do I choose between Lipofectamine 3000, Lipofectamine 2000, and Lipofectamine LTX? Product FAQ

Answer

Lipofectamine 3000 is our best transfection reagent and we highly recommend this over all other reagents for plasmid DNA delivery for a broad range of cell types which includes:

- 60 cell lines (i.e., cancer, fibroblasts, neural, etc.)
- Easy-to-transfect cells
- Hard-to-transfect cells (i.e., neural, stem, blood-derived)
- Suspension cells
- Primary cells
- iPSCs
Please see our table of validated cell lines (https://www.thermofisher.com/us/en/home/brands/product-brand/lipofectamine/lipofectamine-3000.html).

Lipofectamine 2000 and Lipofectamine LTX are alternative options that may work better for certain cell types. For addressing cytotoxicity issues, Lipofectamine 3000 is our most gentle reagent with significantly reduced cytotoxicity levels and improved transfection performance. Both Lipofectamine 3000 and Lipofectamine 2000 may be used for co-transfection (siRNA and plasmid DNA) experiments.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

Answer Id: E9006

Was this answer helpful?

Yes No

Thank you for your response

What is the PLUS Reagent? Product FAQ

Answer

PLUS Reagent is a proprietary reagent for pre-complexing DNA. It enhances cationic lipid-mediated transfection of DNA into many cultured eukaryotic cells when used in conjunction with a transfection reagent. Use PLUS Reagent to enhance transfection results with Lipofectamine Reagent, Lipofectamine LTX Reagent, Lipofectin Reagent, Cellfectin Reagent, and Oligofectamine Reagent. Lipofectamine LTX is offered in combination with the PLUS Reagent in Cat. Nos. A12621, 15338030, and 15338100. PLUS Reagent is also offered as a stand-alone product (Cat. No. 11514015). We do not recommend using the PLUS Reagent with Lipofectamine 2000 or Lipofectamine 3000.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9026

Was this answer helpful?

Yes No

Thank you for your response

Can I use Lipofectamine LTX for transfection of suspension cells, other hard-to-transfect cells, and primary cells? Product FAQ

Answer

We recommend using Lipofectamine 3000 which demonstrates much higher transfection efficiency and protein expression over all other transfection reagents for suspension, hard-to-transfect, and primary cells. Review Lipofectamine 3000 data for over 60 cell lines here https://www.thermofisher.com/us/en/home/brands/product-brand/lipofectamine/lipofectamine-3000.html. For highest transfection efficiencies, we recommend the Neon Transfection System for Jurkat (94%) and K562 (90%) cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9024

Was this answer helpful?

Yes No

Thank you for your response

Is Lipofectamine 3000 the same as Lipofectamine 2000? What about Lipofectamine LTX, Lipofectamine PLUS, and Lipofectin? Product FAQ

Answer

All of our lipid reagents have different cationic-lipid formulations, each with unique properties and specific applications. Lipofectamine 3000 provides the best transfection performance with lowest cytotoxicity for plasmid DNA and RNAi delivery for all cell types. Lipofectamine LTX was formerly designed for delivery of plasmid DNA with minimal cytotoxicity. Lipofectamine PLUS is a discontinued transfection reagent, although the PLUS Reagent is available and sold separately (Cat. No. 11514-015). Lipofectin was originally launched in the late 1980s and is considered our very first transfection reagent. We continue to offer these products for customers who prefer the older formulations, but recommend that all new users try Lipofectamine 3000 first for optimal performance and lowest toxicity.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E8976

Was this answer helpful?

Yes No

Thank you for your response

Results per page