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Regulatory statements vary by product. Please see product-specific literature to determine product use.

What is the Invitrogen Lipofectamine MessengerMAX Transfection Reagent? Product FAQ

Answer

Lipofectamine MessengerMAX Transfection Reagent is an animal origin-free transfection reagent, especially formulated for the delivery of mRNA, small RNA (eg.,CRISPR IVT gRNA, siRNA, or miRNA), and short dsDNA or HDR templates (0.5-1 kb). Lipofectamine MessengerMAX Reagent is an excellent reagent choice for CRISPR-mediated genome editing applications.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E10383

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How easy is it to use Lipofectamine MessengerMAX Transfection Reagent? Product FAQ

Answer

Lipofectamine MessengerMAX Transfection Reagent has a very simple one-tube protocol (http://tools.thermofisher.com/content/sfs/manuals/Lipofectamine_MessengerMAX_man.pdf). The mRNA can either be transcribed using the mMESSAGE mMACHINE ULTRA T7 Transcription Kit or can be purchased ready-to-use (e.g., GeneArt CRISPR Nuclease mRNA, Cat. No. A25640).

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9031

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Which cell types can be used with Lipofectamine MessengerMAX Transfection Reagent? Product FAQ

Answer

Lipofectamine MessengerMAX Reagent demonstrates low toxicity and high transfection efficiency for all cell types (easy or difficult-to-transfect, primary, and stem cells).

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E10385

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Can I deliver single-stranded oligonucleotides with Lipofectamine MessengerMAX Transfection Reagent? Product FAQ

Answer

We recommend using Invitrogen Lipofectamine RNAiMAX Reagent for the delivery of oligos. We do not recommend using Lipofectamine MessengerMAX Reagent for the delivery of single-stranded oligonucleotides for genome editing purposes; literature demonstrates that dsDNA HDR templates provide better directed results.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E10387

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When do you recommend using the Lipofectamine MessengerMAX Transfection Reagent? Product FAQ

Answer

The Lipofectamine MessengerMAX Transfection Reagent is specifically designed for transfection of mRNA into cells. It offers up to 5X the transfection efficiency of DNA transfection reagents in neurons and a broad spectrum of primary cells. This is possible because our novel lipid nanoparticle technology is optimized to deliver the highest amount of mRNA possible without the nuclear entry step that is required with DNA. The added advantage is that protein expression is faster with no risk of genomic integration. Additionally, up to 10X higher cleavage efficiency can be achieved using GeneArt Platinum Cas9 Nuclease for CRISPR gene editing applications.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9028

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Can I use Lipofectamine MessengerMAX Transfection Reagent to deliver plasmid DNA? Product FAQ

Answer

Yes, Lipofectamine MessengerMAX Transfection Reagent can deliver plasmid DNA in combination with mRNA (i.e., in CRISPR); however, Lipofectamine 3000 Transfection Reagent is best optimized for plasmid DNA delivery and superior DNA transfection performance.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9032

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When would I use Lipofectamine MessengerMAX Reagent over other transfection reagents? Product FAQ

Answer

Lipofectamine MessengerMAX Regent is an excellent reagent for co-delivery of Invitrogen GeneArt CRISPR Cas9 mRNA with in vitro transcribed gRNA for geneome editing purposes. Lipofectamine MessengerMAX Reagent has the added benefit of flexibly delivering short dsDNA or HDR templates (0.5-1 kb) which can be ordered through the Invitrogen GeneArt Strings services.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E10384

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Where can I purchase a GFP mRNA positive control to use with Lipofectamine MessengerMAX Transfection Reagent? Product FAQ

Answer

We have validated the TriLink EGFP mRNA Control (Cat. No. L-6101) in-house. As this was discontinued, the alternative is Tri-Link CleanCap EGFP mRNA (Cat. No. L-7201).

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9030

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How do I use Lipofectamine MessengerMAX Reagent to deliver both my GeneArt Cas9 mRNA and IVT gRNA together? Product FAQ

Answer

Lipofectamine MessengerMAX Reagent may be used for both single gRNA delivery or multiplexing (gRNA for multiple targets) purposes with high transfection efficiency when used with GeneArt Cas9 mRNA. For a detailed protocol, please refer to the GeneArt Cas9 mRNA manual (https://www.thermofisher.com/order/catalog/product/A25640?ICID=search-a25640). We recommend using either the Invitrogen GeneArt Genomic Cleavage Detection Kit (Cat. No. A24732) or Invitrogen GeneArt Genomic Cleavage Selection Kit (Cat. No. A27663) for mutant analysis.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E10386

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Does the Lipofectamine MessengerMAX Transfection Reagent kit include reagents for in vitro transcription of mRNA? Product FAQ

Answer

Although it does not, we recommend the mMESSAGE mMACHINE T7 ULTRA Transcription Kit (Cat. No. AMB1345) to generate capped and tailed mRNA for highest mRNA stability.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9029

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What reagent should I use to transfect the Invitrogen GeneArt CRISPR Nuclease mRNA? Product FAQ

Answer

We recommend Invitrogen Lipofectamine MessengerMAX reagent.

Answer Id: E10166

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I have been getting some toxicity in my mRNA transfections using Lipofectamine MessengerMAX Transfection Reagent. What would you recommend for minimizing toxicity? Product FAQ

Answer

Consider including appropriate 5' UTR and 3' UTR sequences into the template design to promote mRNA stability and acceptance by the cell. Use high purity in vitro transcribed mRNA purified with the MegaClear Transcription Cleanup Kit. Ensure A260/280 ratios are between 1.8 and 2.1. Check for mRNA quality and size by agarose gel electrophoresis. In some cases, consider incorporating chemically modified nucleotides in the transcription reaction. Cell number, mRNA, and lipid quantities may need to be adjusted. Ideal viable cell density on the day of transfection is between 70 and 90% confluence. For transfection optimization tips, please visit: thermofisher.com/transfectionsupport.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9069

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What are the main differences between Vivofectamine VF221 Lung in vivo RNA Delivery Reagent and Lipofectamine MessengerMAX Transfection Reagent? Product FAQ

Answer

Broadly speaking, our Lipofectamine brand transfection reagents are optimized for in vitro transfection, while the Vivofectamine product provides in vivo or ex vivo delivery solutions. Lipofectamine MessengerMAX Transfection Reagent is specifically designed for delivering mRNA payload in vitro, while Vivofectamine VF221 Lung in vivo RNA Delivery Reagent is optimized for in vivo mRNA or siRNA delivery to the lung tissue.

We also have the TransfectionSelect tool to help you choose the best delivery solution for your application: https://www.thermofisher.com/order/transfectionselect/?SID=fr-transfection-main#!/

Answer Id: E21210

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Which transfection reagent should I use to deliver the GeneArt CRISPR nuclease mRNA? Product FAQ

Answer

Lipofectamine MessengerMAX Transfection Reagent is especially formulated for the delivery of both mRNA and gRNAs for all cell types (easy or difficult-to-transfect, primary, and stem cells).

Answer Id: E10390

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Do you offer a transfection reagent for transfection of mRNA? Product FAQ

Answer

Yes, Lipofectamine MessengerMAX Transfection Reagent is our best reagent for mRNA delivery and shows up to 5Xs the transfection efficiency of DNA transfection reagents in neurons and primary cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Answer Id: E9009

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