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Large Vessel Endothelial Supplement (LVES) (Gibco™)

Gibco® Large Vessel Endothelial Supplement (LVES) is a 50X, complete, ready-to-use media supplement optimized for use with Gibco® Medium 200 and Medium 200PRF for culture of primary human umbilical vein endothelial cells (HUVEC), human aortic endothelial cells (HAEC), and human pulmonary artery endothelial cells (HPAEC). This blended supplement has been designed to deliver optimal cell growth and to enable superior cell performance for angiogenesis applications, such as cell migration and tube formation. LVES contains fetal bovine serum, hydrocortisone, human epidermal growth factor, basic fibroblast growth factor, heparin, and ascorbic acid.

• Perform tube formation, cell invasion, cell migration, and cell proliferation functional assays
• Achieve superior growth rates of endothelial cells compared to low serum growth supplement (LSGS)
• Expand primary large vessel endothelial cells without genomic alteration

Verified Performance in Key Angiogenesis Applications
Large Vessel Endothelial Supplement is designed to deliver optimal performance in tube formation, cell invasion, cell migration, and cell proliferation assays when used in conjunction with Medium 200, human umbilical vein endothelial cells, and Geltrex® LDEV-Free Reduced Growth Factor Basement Membrane Matrix. These items and a detailed protocol are available in a convenient Angiogenesis Starter Kit or they can be purchased separately.

Maximum Primary Cell Growth with One Blended Supplement
Large Vessel Endothelial Supplement is a complete, ready-to-use blend of growth supplements optimized to deliver>4 x 104 HUVEC per cm2 when used with Medium 200 or Medium 200PRF. Cell phenotype is retained and verified positive for von Willebrand factor (vWf) and CD31, negative for a-actin, and positive for dil-Ac-LDL uptake via ICC.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Angiogenesis Starter Kit (Gibco™)

The Gibco® Angiogenesis Starter Kit contains the reagents and protocols necessary to perform various angiogenesis assays. Designed to address the needs of both the occasional user as well as the most experienced researcher, this combination of high quality products is easy to use and cost effective. Detailed protocols for the tube formation assay and cell migration are available below.

What’s Included:

• Gibco® Large Vessel Endothelial Supplement (LVES), 50X, 11 ml, ready to use
• Gibco® Medium 200 basal media, 500 ml
• Gibco® Human Umbilical Vein Endothelial Cells (HUVEC), 1 vial, 5 x 105 cells
• Geltrex® LDEV-Free Reduced Growth Factor Basement Membrane Matrix, 5 ml

Large Vessel Endothelial Supplement and Medium 200
Gibco® LVES is a 50X, complete, ready-to-use media supplement optimized for use with Gibco® Medium 200. This blended supplement has been designed to deliver optimal cell growth and to enable superior cell performance for angiogenesis applications such as cell migration and tube formation. LVES contains fetal bovine serum, hydrocortisone, human epidermal growth factor, basic fibroblast growth factor, heparin, and ascorbic acid.

Human Umbilical Vein Endothelial Cells
Gibco® Human Umbilical Vein Endothelial Cells are cryopreserved at the end of the primary culture. Upon thawing, they are guaranteed to be >70% viable and capable of a minimum of 16 population doublings. Cells are derived from tissue obtained from accredited institutions with IRB-approved donor programs. Quality control of these cells includes testing for mycoplasma, sterility, endotoxin, hepatitis B and C, and HIV. Additionally, the cells are positive for von Willebrand factor (vWf), positive for CD31, negative for a-actin, and positive for dil-Ac-LDL uptake.

Geltrex® LDEV-Free Matrix
Geltrex® LDEV-Free Reduced Growth Factor Basement Membrane Matrix is a soluble, reduced growth factor, lactate dehydrogenase-elevating virus-free basement membrane extract used in a wide variety of cell culture applications for promotion and maintenance of many cell types, including primary epithelial cells, endothelial cells, and stem cells. It can be diluted for use as a coating matrix for culture vessels or used undiluted as a thick gel preparation to create a more physiologically relevant environment for angiogenesis assays.