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STAT1 Recombinant Human Protein

Recombinant human STAT1 is phosphorylatable in vitro, using either immunoprecipitated JAK1 or JAK2, or whole cell extracts derived from interferon-gamma-stimulated HEK 293 cells.

Format/Formulation: Protein expressed in E. coli, purity (>95%)

Pierce™ Recombinant GFP Protein (Thermo Scientific™)

Recombinant GFP (Green Fluorescent Protein) is intended as a convenient positive control for the in vitro synthesis of GFP using the Thermo Scientific 1-Step Human Coupled IVT Kit.

More Product Data
Protocol for in vitro protein expression using mRNA templates
Glycoprotein expression in a human IVT system

Recombinant Protein G-HRP (Invitrogen™)

Recombinant protein G is produced in a strain of E. coli which contains the gene for protein G from Streptococcus sp. This protein G is conjugated with highly purified horseradish peroxidase (RZ > 3.0) and is in enyme conjugate stabilizer containing 0.19% Kathon CG/ICP.

Recombinant protein G binds to the Fc region of many types of immunoglobulin G including:

• all four subclasses of human IgG
• mouse IgG
• rabbit IgG
• guinea pig IgG
• rat IgG
• goat IgG
• sheep IgG
• cow IgG
• horse IgG

Recombinant protein G does not bind to IgG from chicken or cat, or to human IgA, IgM, or serum albumin. It binds weakly to IgG from dog.

Frizzled 4 Recombinant Human Protein (Gibco™)

Recombinant Frizzled 4 is a bioactive protein intended for use in cell culture applications. FZD4 is a member of the Frizzled family, which are 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. Wnt signaling is involved in several developmental processes.

SLAMF6 Recombinant Human Protein, His Tag

SLAM family member 6 (SLAMF6), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human SLAMF6 (Q96DU3-1) (Met 1-Met 226) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its ability to bind biotinylated recombinant human SH2D1A in a functional ELISA.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

C1QBP Recombinant Human Protein, His tag

Complement component 1, Q subcomponent Binding protein (C1QB), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of human C1QBP (NP_001203.1) (His 75-Gln 282) fused to two Met at N-terminus and a polyhistidinetag at the C-terminus.

Activity: The activity of this protein has not been tested.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

This protein is manufactured by Sino Biological Inc.

IgG1-Fc Recombinant Human Protein

Manufactured by Sino Biological (China); Distributed by Life Technologies.

Immunoglobulin gamma-1 heavy chain constant region, partial (IgG1-Fc), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein standard and in cell biology research applications.

This recombinant human protein is expressed from a DNA sequence encoding the human IgG1-Fc region (AAC82527.1), containing residues 99 to 330, including a 103 Cys/Ser mutation.

N-terminal Sequence Analysis: Glu 99.

Activity:
1. Measured by its ability to bind human CD16a-His (Cat:10389-H08H1) in a functional ELISA.
2. Measured by its ability to bind human CD16a-AVI-His (Cat:10389-H27H) in a functional ELISA.
3.Measured by its ability to bind human CD16a-His (Cat:10389-H08H) in a functional ELISA.

Formulation: lyophilized in PBS, pH 7.4, 5% mannitol, 5% trehalose, and 0.02% Tween®-80.

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: expires one year from date of receipt when stored as instructed.

Background
Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). Recombinant IgG Fc is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases.

You may also be interested in the following IgG1 products:
ELISA Kit
Primary Antibodies
Protein Labeling Kits

Frizzled 7 Recombinant Human Protein (Gibco™)

Recombinant Human Frizzled-7 (FZD7) is a bioactive recombinant protein intended for use in cell culture applications. FZD7 is a member of the Frizzled family, which are 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. Wnt signaling is involved in several developmental processes.

The protein sequence of Frizzled-7 corresponds to the extracellular region of mature Human Frizzled 7, aa 33-185, fused to the Fc region of Human IgG1.

The equilibrium binding constant, KD, of Human Frizzled 7 was measured at less than 10nM using mouse Wnt3a as the ligand. Measurements were made using the ForteBio Octet optical biosensor instrument.

β Amyloid [1 42] PTD Human Protein (Invitrogen™)

Major constituent of plaques and tangles that occur in Alzheimer's disease (AD) patients.

Format/Formulation: Lyophilized trifluoroacetate salt

Molecular Weight: 4515

HA Synthetic Peptide (Thermo Scientific™)

Lyophilized peptide with the sequence N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C. Molecular weight: 1102.2 g/mol

26184 can be used to competitively elute HA-tagged fusion proteins from immobilized anti-HA affinity resin. Use 4 X 1 column volumes of 1 mg/ml HA peptide solution to elute HA-tagged proteins. Elution yields will increase by incubating the peptide on the column for 10-15 minutes at 30-37ºC for each elution fraction.

Reconstitute to 1 mg/ml using TBS or distilled water.

The HA peptide, NH2-YPYDVPDYA-COOH, is a synthetic peptide used to competitively elute fusion proteins containing the influenza hemagglutinin (HA) tag bound to immobilized anti-HA affinity resins.

protein A, Alexa Fluor™ 546 conjugate (Invitrogen™)

Our Alexa Fluor 546 protein A conjugate binds to the Fc portion of various immunoglobulins from several different species. This exceptionally bright, orange-fluorescent probe (absorption/emission maxima ~556/573 nm) can be used to visualize antibodies in a manner analogous to a labeled species-specific secondary antibody.

APOE Recombinant Human Protein, N-TRX.His tag

Apolipoprotein E (APOE), recombinant human protein is supplied as a frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the human APOE (P02649) (Lys 19-His 317) fused to a Trx and a polyhistidine tag at the N-terminus.

Activity: The activity of this protein has not been tested.

Formulation: PBS, 40% Glycerol, 0.5 mM TCEP, pH 7.4.

Reconstitution: No reconstitution needed. Store at -80°C. Thaw protein on ice before use and avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

Frizzled 1 Recombinant Human Protein (Gibco™)

Recombinant Frizzled 1 is a bioactive protein intended for use in cell culture applications. FZD1 is a member of the Frizzled family, which are 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. Wnt signaling is involved in several developmental processes.

EFNB1 (EphrinB1) Recombinant Human Protein, His Tag

Ephrin-B1 (EFNB1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 232) of human EFNB1 (NP_004420.1) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human EFNB1 at 10 µg/ml(100 µl/well) can bind human EPHB6 with a linear range of 0.16-4 µg/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

CR2 Recombinant Human Protein, His Tag

Complement component (3d/Epstein Barr virus) receptor 2 (CR2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the human CR2 (P20023-1) extracellular domain (Met 1-Arg 971) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA
. Immobilized human CR2 at 2 µg/ml (100 µl/well) can bind biotinylated human CD9.
The EC50 of biotinylated human CD9 is 1.76 µg/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.