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Low Protein Binding Collection Tubes (1.5 mL) (Thermo Scientific™)

Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (1.5 mL) help minimize protein sample loss during sample manipulation and are ideal in applications where processing steps are extensive.

Durable—able to tolerate centrifuge speeds up to 20,000 RCF
Compatible—suitable for IP and mass spectrometry applications
Nuclease-free—certified RNase/DNase-free

The Pierce Low Protein Binding Microcentrifuge Tubes help minimize sample loss in mass spectrometry and immunoprecipitation/co-immunoprecipitation applications. The polypropylene tubes are certified as DNase/RNase-free and are conveniently packaged (250 tubes per package) to minimize contamination.

Immuno Tubes and Stoppers (Thermo Scientific™)

Reproduce results time after time with Thermo Scientific™ Immuno Tubes and Stoppers, ideal for ELISA, solid phase RIA and liquid phase RIA assays.

Ion Template Preparation Tubes for PGM™ System (Ion Torrent™)

These customized tubes and caps are for use with the ULTRA-TURRAX® Tube Drive from IKA® for the PGM™ System to create emulsion PCR reactions.

The ULTRA-TURRAX® Tube Drive from IKA® for the Personal Genome Machine™System is a sample preparation instrument for use in the Personal Genome Machine™ sequencing workflow. This customized benchtop device is used for the preparation of appropriately sized reactors for emulsion PCR.

NOTE: 4467226 replaces 4400401.

For Research Use Only. Not for use in diagnostics procedures.

Sterilin™ Plastic Culture Tubes (Thermo Scientific™)

Gain versatility in culture applications with these plastic culture tubes. With a patch for labeling and a two-position cap, the tubes are ideal for suspension cell culture.

Samco™ 16 × 125mm Disposable Culture Tubes (DCTs) (Thermo Scientific™)

Choose the material that suits your needs with these disposable culture tubes, in clarified polypropylene for strength and resistance, or polystyrene for superior clarity.

BeadRetriever™ Tubes & Tips (Applied Biosystems™)

The tube strips are designed for processing and isolation of target molecules; one sample uses one tube strip which is made up of five tubes. The disposable plastic tips are designed for shielding the magnetic surface from contact with the sample. The BeadRetriever™ System utilize low binding, disposable plastics materials for optimal magnetic particle processing.

Sterilin™ Round Base Rimless Borosilicate Glass Rimless Tubes (Thermo Scientific™)

Trust these round-base borosilicate glass tubes in high temperature, aggressive chemical applications. Ideal for use with samples that are sensitive to plastic tube leachables.

Elution Tubes (2.0 mL) (Invitrogen™)

Ambion® RNase-free Elution Tubes are guaranteed RNase- and DNase-free and can accommodate RNAqueous® (SKU# AM1912) and Poly(A)Purist™ (SKU# AM1916) spin cartridges. 2.0 mL tubes are provided in a box of 100.

Each lot of tubes is certified RNAse- and DNase-free.

Samco™ 17 × 100mm Disposable Culture Tubes (DCTs) (Thermo Scientific™)

Choose the material that suits your needs with these disposable culture tubes, in clarified polypropylene for strength and resistance, or polystyrene for superior clarity.

Sterilin™ Caps for Glass Tubes (Thermo Scientific™)

Secure your samples with these autoclavable screw-thread caps for glass tubes.

Sterilin™ Borosilicate Glass Culture Tubes (Thermo Scientific™)

Rely on chemical and temperature resistant borosilicate glass culture tubes in your tough applications. Available with flat or round bottom, threaded to accept a screw cap.

Sterilin™ Round Base Soda Glass Tubes (Thermo Scientific™)

Rely on these round base glass tubes for excellent chemical resistance for samples to 2mL or 4mL. Test tube profile for streamlined handling and excellent mixing capability.

PureLink™ Viral Collection Tubes (Invitrogen™)

The PureLink® Viral RNA/DNA Mini Kit is a nucleic acid purification system designed for fast and easy isolation of viral RNA or DNA from cell-free samples, such as serum, plasma, and cerebrospinal fluid. Starting sample volumes can be as high as 500 µl and elution volumes as low as 10 µl, allowing for a 50-fold concentration of nucleic acids. The higher target concentration obtained using the PureLink® Viral RNA/ DNA Mini Kit provides greater sensitivity and accuracy in downstream detection procedures such as real-time PCR or endpoint analysis. Specially formulated buffers enable the purification of RNA or DNA, allowing one system to be used for all your virus extraction samples. In addition, the kit provides sufficient quantities of lysis buffer to address large starting sample volumes.

Benefits:
• Yields highly concentrated viral nucleic acids for greater sensitivity in detection - start with 500 µl of sample and elute in just 10 µl volume
• One kit provides a complete solution for virus extraction - both RNA and DNA viruses can be extracted and concentrated
• Spin column technology - achieve fast and easy purification with excellent reproducibility (Figure 1)
• Produces high-quality DNA and RNA - ideal for downstream applications such as real-time PCR and endpoint analysis
Ct values were consistently lower for samples prepared with the PureLink® Viral RNA/DNA Mini Kit (Figure 2), indicating greater sensitivity.

In addition, the quantitation was accurate down to 20 pfu compared to samples prepared with the kit from supplier Q, which leveled off at 200 pfu. Results indicate an improvement in the recovery of viral RNA as well as greater overall consistency of quantitative amplification.

Lower Ct values were obtained with DNA prepared with the PureLink ™ Viral RNA/DNA Mini Kit as compared to those obtained using the kit from supplier Q (Figure 3), indicating greater sensitivity of detection of viral DNA. The data demonstrate that the PureLink® Viral RNA/DNA Mini Kit can recover DNA down to 20 pfu at significantly lower Ct values compared to the competitor’s kit.

The PureLink® Viral RNA/DNA Mini Kit yielded target molecules of consistently lower Ct values compared to the EZ1 Kit; in some cases as much as 4 cycles earlier (Figure 4). The arrows indicate samples that failed to produce a Ct value. In total, 14 out of 48 samples failed to produce a Ct when processed on the BioRobot EZ1 (29%), while the PureLink® Viral RNA/DNA Kit failed to provide a Ct value in only 3 samples (6%), of which both replicates extracted with the EZ1 Kit also failed to produce a Ct value.

For enhanced viral RNA or DNA detection, the PureLink® Viral RNA/DNA Mini Kit has been demonstrated to give lower Ct values and increased nucleic acid recovery when compared to competitor products using volumes recommended by the supplier. The flexibility of the PureLink® Viral RNA/DNA Mini Kit with respect to sample volume, elution volume, and virus type makes it the best solution for purifying viral RNA or DNA.

Contents and storage:
The PureLink® Viral RNA/DNA Mini Kit contains Mini Spin Columns in Collection Tubes, Collection Tubes, Recovery Tubes, Lyophilized Carrier RNA, Proteinase K, Lysis Buffer, 5X Wash Buffer II, and RNase-free Water. Each kit contains sufficient material for 50 purifications. The kit is shipped at room temperature; upon receipt, store the lyophilized carrier RNA at -20°C and the proteinase K at 4°C. Stored as directed, the kit is stable for 1 year.

Invitrogen™ Phasemaker™ Tubes (Invitrogen™)

Invitrogen™ Phasemaker™ Tubes contain a thick liquid polymer that separates and completely isolates the upper aqueous phase of a TRIzol® Reagent mix from the organic phase underneath, greatly simplifying removal of the upper phase containing the RNA.

• Pre-dispensed, 2 mL tubes
• Exclusively for RNA extraction
• For use with TRIzol Reagent, TRIzol® LS Reagent, and TRIzol® Plus Purification Kit only
• Simplifies removal of aqueous phase
• Helps increase RNA yield up to 30%
• Does not interfere with downstream experiments

The polymer in Phasemaker Tubes is heavier than the aqueous phase of the TRIzol Reagent mix, but lighter than the organic phase, so after centrifugation it positions itself between these two layers. As a thick polymer, it creates a tight seal between the two phases, easing removal of the aqueous phase and helping to reduce the risk of carrying over contaminants from the organic layer, thereby increasing RNA recovery up to 30%.

The polymer in the Phasemaker Tubes is inert and does not interfere with standard RNA applications. Isolated RNA can be used in RT-PCR, Northern blot analysis, dot blot hybridization, poly(A)+ selection, in vitro translation, RNase protection assay, and molecular cloning.

Phasemaker Tubes accommodate RNA extraction using TRIzol Reagent, TRIzol LS Reagent, and TRIzol Plus Purification Kit only. They are not recommended for use with other organic reagents as the density of those reagents might not be suitable. Phasemaker Tubes are not for use in DNA or protein isolation, but are intended for RNA extraction only.

Phasemaker Tubes work with fresh or frozen samples, including tissue and blood. They also work with samples previously treated with RNAlater™ RNA stabilization solution. Phasemaker Tubes are already pre-dispensed; the tube size is 2 mL.

Samco™ 12 × 17mm Disposable Culture Tubes (DCTs) (Thermo Scientific™)

Choose the material that best suits your needs with these DCTs, available in clarified polypropylene for strength and resistance, or polystyrene for superior clarity.