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Immuno Tubes and Stoppers (Thermo Scientific™)

Reproduce results time after time with Thermo Scientific™ Immuno Tubes and Stoppers, ideal for ELISA, solid phase RIA and liquid phase RIA assays.

Vacuum Hydrolysis Tube, 1 mL (Thermo Scientific™)

Thermo Scientific Pierce Vacuum Hydrolysis Tube, 1 mL is a high-quality glass tube designed for heated, acid-based, vacuum hydrolysis of proteins and peptides for amino acid analysis.

Features of Vacuum Hydrolysis Tube, 1 mL:

• Saves 10-18 hours on hydrolysis procedures when combined with 6N HCl (Part No. 24308)
• Provides for hydrolysis in 6 hours when heated at 150°C in a dry block heater (Note: the upper temperature limit of Vacuum Hydrolysis Tubes is 260°C in a block heater but only 110°C in an oven)
• Optimal hydrolysis control when used with Reacti-Therm Dry Block Heating System
• Compatible-fitting are available for use with Thermo Scientific Reacti-Therm Heating Modules

Applications of Vacuum Hydrolysis Tube:
• Protein/peptide hydrolysis
• Sample concentration
• Lyophilization
• Hydrazinolysis

Vacuum Hydrolysis Tubes are precision-designed for use in protein hydrolysis and subsequent analysis of protein and peptide amino acid compositions. The heavy-walled borosilicate glass construction ensures complete compatibility with all commonly used acid hydrolysis reagents. Acid hydrolysis at elevated temperatures, in vacuo, will hydrolyze most proteins and peptides to their amino acid components without significant decomposition.

Vacuum hydrolysis methods commonly use 6N constant boiling hydrochloric acid (HCl), a mixture of HCl and propionic acid, 3N mercaptoethanesulfonic acid or 4N methanesulfonic acid. The constant boiling HCl method uses standard protein hydrolysis at 105 to 110°C for 16 to 24 hours. Using the HCl/propionic acid method, hydrolysis times can be shortened to 15 minutes at 160°C. Tryptophan is easily degraded by acid hydrolysis in 6N HCl. The presence of oxygen, heavy metals and carbohydrates are contributing factors to this degradation. A 22-hour hydrolysis method was developed to improve tryptophan recovery from protein hydrolysis samples by using 3N mercaptoethanesulfonic acid. A second method to improve tryptophan recovery involves hydrolysis at 115°C using 4N methanesulfonic acid.

Related Products
Vacuum Hydrolysis Tube, 6 mL
Vacuum Hydrolysis Tube, 18 mL

Vacuum Hydrolysis Tube, 6 mL (Thermo Scientific™)

Thermo Scientific Pierce Vacuum Hydrolysis Tube, 6 mL is a high-quality glass tube designed for heated, acid-based, vacuum hydrolysis of proteins and peptides for amino acid analysis.

Features of Vacuum Hydrolysis Tube, 6 mL:

• Saves 10-18 hours on hydrolysis procedures when combined with 6N HCl (Part No. 24308)
• Provides for hydrolysis in 6 hours when heated at 150°C in a dry block heater (Note: the upper temperature limit of Vacuum Hydrolysis Tubes is 260°C in a block heater but only 110°C in an oven)
• Optimal hydrolysis control when used with Reacti-Therm Dry Block Heating System
• Compatible-fitting are available for use with Thermo Scientific Reacti-Therm Heating Modules

Applications of Vacuum Hydrolysis Tube:
• Protein/peptide hydrolysis
• Sample concentration
• Lyophilization
• Hydrazinolysis

Vacuum Hydrolysis Tubes are precision-designed for use in protein hydrolysis and subsequent analysis of protein and peptide amino acid compositions. The heavy-walled borosilicate glass construction ensures complete compatibility with all commonly used acid hydrolysis reagents. Acid hydrolysis at elevated temperatures, in vacuo, will hydrolyze most proteins and peptides to their amino acid components without significant decomposition.

Vacuum hydrolysis methods commonly use 6N constant boiling hydrochloric acid (HCl), a mixture of HCl and propionic acid, 3N mercaptoethanesulfonic acid or 4N methanesulfonic acid. The constant boiling HCl method uses standard protein hydrolysis at 105 to 110°C for 16 to 24 hours. Using the HCl/propionic acid method, hydrolysis times can be shortened to 15 minutes at 160°C. Tryptophan is easily degraded by acid hydrolysis in 6N HCl. The presence of oxygen, heavy metals and carbohydrates are contributing factors to this degradation. A 22-hour hydrolysis method was developed to improve tryptophan recovery from protein hydrolysis samples by using 3N mercaptoethanesulfonic acid. A second method to improve tryptophan recovery involves hydrolysis at 115°C using 4N methanesulfonic acid.

Related Products
Vacuum Hydrolysis Tube, 1 mL
Vacuum Hydrolysis Tube, 18 mL

Vacuum Hydrolysis Tube, 18 mL (Thermo Scientific™)

Thermo Scientific Pierce Vacuum Hydrolysis Tube, 18 mL is a high-quality glass tube designed for heated, acid-based, vacuum hydrolysis of proteins and peptides for amino acid analysis.

Features of Vacuum Hydrolysis Tube, 18 mL:

• Saves 10-18 hours on hydrolysis procedures when combined with 6N HCl (Part No. 24308)
• Provides for hydrolysis in 6 hours when heated at 150°C in a dry block heater (Note: the upper temperature limit of Vacuum Hydrolysis Tubes is 260°C in a block heater but only 110°C in an oven)
• Optimal hydrolysis control when used with Reacti-Therm Dry Block Heating System
• Compatible-fitting are available for use with Thermo Scientific Reacti-Therm Heating Modules

Applications of Vacuum Hydrolysis Tube:
• Protein/peptide hydrolysis
• Sample concentration
• Lyophilization
• Hydrazinolysis

Vacuum Hydrolysis Tubes are precision-designed for use in protein hydrolysis and subsequent analysis of protein and peptide amino acid compositions. The heavy-walled borosilicate glass construction ensures complete compatibility with all commonly used acid hydrolysis reagents. Acid hydrolysis at elevated temperatures, in vacuo, will hydrolyze most proteins and peptides to their amino acid components without significant decomposition.

Vacuum hydrolysis methods commonly use 6N constant boiling hydrochloric acid (HCl), a mixture of HCl and propionic acid, 3N mercaptoethanesulfonic acid or 4N methanesulfonic acid. The constant boiling HCl method uses standard protein hydrolysis at 105 to 110°C for 16 to 24 hours. Using the HCl/propionic acid method, hydrolysis times can be shortened to 15 minutes at 160°C. Tryptophan is easily degraded by acid hydrolysis in 6N HCl. The presence of oxygen, heavy metals and carbohydrates are contributing factors to this degradation. A 22-hour hydrolysis method was developed to improve tryptophan recovery from protein hydrolysis samples by using 3N mercaptoethanesulfonic acid. A second method to improve tryptophan recovery involves hydrolysis at 115°C using 4N methanesulfonic acid.

Related Products
Vacuum Hydrolysis Tube, 1 mL
Vacuum Hydrolysis Tube, 6 mL

Nunc™ Cell Culture Tubes (Thermo Scientific™)

Perform adherent cell cultures in biotechnology, pharmaceutical and research laboratories with Thermo Scientific™ Nunc™ Cell Culture Tubes.

Sterilin™ Round Base Rimless Borosilicate Glass Rimless Tubes (Thermo Scientific™)

Trust these round-base borosilicate glass tubes in high temperature, aggressive chemical applications. Ideal for use with samples that are sensitive to plastic tube leachables.

Low Protein Binding Collection Tubes (2.0 mL) (Thermo Scientific™)

Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (2.0 mL) help minimize protein sample loss during sample manipulation and are ideal in applications where processing steps are extensive.

Durable—able to tolerate centrifuge speeds up to 20,000 RCF
Compatible—suitable for IP and mass spectrometry applications
Nuclease-free—certified RNase/DNase-free

The Pierce Low Protein Binding Microcentrifuge Tubes help minimize sample loss in mass spectrometry and immunoprecipitation/co-immunoprecipitation applications. The polypropylene tubes are certified as DNase/RNase-free and are conveniently packaged (250 tubes per package) to minimize contamination.

Sterilin™ Caps for Glass Tubes (Thermo Scientific™)

Secure your samples with these autoclavable screw-thread caps for glass tubes.

Samco™ 12 × 75mm Disposable Culture Tubes (DCTs) (Thermo Scientific™)

Get superior clarity with these chemical-free and biologically inert polystyrene DCTs designed for most laboratory applications.

Low Protein Binding Collection Tubes (1.5 mL) (Thermo Scientific™)

Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (1.5 mL) help minimize protein sample loss during sample manipulation and are ideal in applications where processing steps are extensive.

Durable—able to tolerate centrifuge speeds up to 20,000 RCF
Compatible—suitable for IP and mass spectrometry applications
Nuclease-free—certified RNase/DNase-free

The Pierce Low Protein Binding Microcentrifuge Tubes help minimize sample loss in mass spectrometry and immunoprecipitation/co-immunoprecipitation applications. The polypropylene tubes are certified as DNase/RNase-free and are conveniently packaged (250 tubes per package) to minimize contamination.

Samco™ 16 × 125mm Disposable Culture Tubes (DCTs) (Thermo Scientific™)

Choose the material that suits your needs with these disposable culture tubes, in clarified polypropylene for strength and resistance, or polystyrene for superior clarity.

Tempus™ Blood RNA Tube (Applied Biosystems™)

Tempus™ Blood RNA Tubes are for stabilization and isolation of total RNA from whole blood for gene expression analysis. Advantages of Tempus™ Blood RNA Tubes:

• Immediately lyse whole blood cells and stabilize RNA in a single step—no RBC lysis or proteinase K treatments required
• Freeze global gene expression profiles for up to five days at room temperature; greater than seven days at 4°C

How it works
Each Tempus™ Blood RNA Tube contains 6 mL Stabilizing Reagent. When the blood (3 mL) is drawn into the tube and mixed with this reagent, lysis occurs almost immediately. The stabilizing reagent inactivates cellular RNases and selectively precipitates RNA; genomic DNA (gDNA) while proteins remain in solution. Blood drawn into the Tempus™ Blood RNA Tube is stable for up to five days at room temperature, or minimally seven days at 4°C.

Accessory product
When the Tempus™ Blood RNA Tubes are used with the Tempus™ 12-Port RNA Isolation Kit (Cat. No. 4378672), RNAs with greater stability and higher yields of RNA are produced (see figure).

Sterilin™ Borosilicate Glass Culture Tubes (Thermo Scientific™)

Rely on chemical and temperature resistant borosilicate glass culture tubes in your tough applications. Available with flat or round bottom, threaded to accept a screw cap.

Samco™ 17 × 100mm Disposable Culture Tubes (DCTs) (Thermo Scientific™)

Choose the material that suits your needs with these disposable culture tubes, in clarified polypropylene for strength and resistance, or polystyrene for superior clarity.

Genetic Analyzer Sample Tubes, 0.5 mL (Applied Biosystems™)

These are sample tubes for use in the 48-sample tray of the 310 Genetic Analyzer.

Use with: 1.0 mL Glass Syringe, 310 Genetic Analyzer Capillary (47 cm, 36 cm wtr), 310 Running Buffer (10X), BigDye® Terminator v1.1 Cycle Sequencing Kit, GeneScan™ 500 ROX™ Size Standard, Hi-Di™ Formamide, MicroAmp® Reaction Tube with Cap (0.2 ml), POP-4™ Polymer for the 310 Genetic Analyzer, POP-6™ Polymer for the 310 Genetic Analyzer, Septa for 0.5 ml Sample Tubes

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.