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Sterilin™ Caps for Glass Tubes (Thermo Scientific™)

Secure your samples with these autoclavable screw-thread caps for glass tubes.

Samco™ 17 × 100mm Disposable Culture Tubes (DCTs) (Thermo Scientific™)

Choose the material that suits your needs with these disposable culture tubes, in clarified polypropylene for strength and resistance, or polystyrene for superior clarity.

Nunc™ 14mL Round-Bottom Tubes (Thermo Scientific™)

Perform critical cellular applications with these 14mL round-bottom tubes.

Invitrogen™ Phasemaker™ Tubes (Invitrogen™)

Invitrogen™ Phasemaker™ Tubes contain a thick liquid polymer that separates and completely isolates the upper aqueous phase of a TRIzol® Reagent mix from the organic phase underneath, greatly simplifying removal of the upper phase containing the RNA.

• Pre-dispensed, 2 mL tubes
• Exclusively for RNA extraction
• For use with TRIzol Reagent, TRIzol® LS Reagent, and TRIzol® Plus Purification Kit only
• Simplifies removal of aqueous phase
• Helps increase RNA yield up to 30%
• Does not interfere with downstream experiments

The polymer in Phasemaker Tubes is heavier than the aqueous phase of the TRIzol Reagent mix, but lighter than the organic phase, so after centrifugation it positions itself between these two layers. As a thick polymer, it creates a tight seal between the two phases, easing removal of the aqueous phase and helping to reduce the risk of carrying over contaminants from the organic layer, thereby increasing RNA recovery up to 30%.

The polymer in the Phasemaker Tubes is inert and does not interfere with standard RNA applications. Isolated RNA can be used in RT-PCR, Northern blot analysis, dot blot hybridization, poly(A)+ selection, in vitro translation, RNase protection assay, and molecular cloning.

Phasemaker Tubes accommodate RNA extraction using TRIzol Reagent, TRIzol LS Reagent, and TRIzol Plus Purification Kit only. They are not recommended for use with other organic reagents as the density of those reagents might not be suitable. Phasemaker Tubes are not for use in DNA or protein isolation, but are intended for RNA extraction only.

Phasemaker Tubes work with fresh or frozen samples, including tissue and blood. They also work with samples previously treated with RNAlater™ RNA stabilization solution. Phasemaker Tubes are already pre-dispensed; the tube size is 2 mL.

Immuno Tubes and Stoppers (Thermo Scientific™)

Reproduce results time after time with Thermo Scientific™ Immuno Tubes and Stoppers, ideal for ELISA, solid phase RIA and liquid phase RIA assays.

Samco™ 12 × 75mm Disposable Culture Tubes (DCTs) (Thermo Scientific™)

Get superior clarity with these chemical-free and biologically inert polystyrene DCTs designed for most laboratory applications.

Tempus™ Blood RNA Tube (Applied Biosystems™)

Tempus™ Blood RNA Tubes are for stabilization and isolation of total RNA from whole blood for gene expression analysis. Advantages of Tempus™ Blood RNA Tubes:

• Immediately lyse whole blood cells and stabilize RNA in a single step—no RBC lysis or proteinase K treatments required
• Freeze global gene expression profiles for up to five days at room temperature; greater than seven days at 4°C

How it works
Each Tempus™ Blood RNA Tube contains 6 mL Stabilizing Reagent. When the blood (3 mL) is drawn into the tube and mixed with this reagent, lysis occurs almost immediately. The stabilizing reagent inactivates cellular RNases and selectively precipitates RNA; genomic DNA (gDNA) while proteins remain in solution. Blood drawn into the Tempus™ Blood RNA Tube is stable for up to five days at room temperature, or minimally seven days at 4°C.

Accessory product
When the Tempus™ Blood RNA Tubes are used with the Tempus™ 12-Port RNA Isolation Kit (Cat. No. 4378672), RNAs with greater stability and higher yields of RNA are produced (see figure).

Samco™ 12 × 17mm Disposable Culture Tubes (DCTs) (Thermo Scientific™)

Choose the material that best suits your needs with these DCTs, available in clarified polypropylene for strength and resistance, or polystyrene for superior clarity.

Vacuum Hydrolysis Tube, 6 mL (Thermo Scientific™)

Thermo Scientific Pierce Vacuum Hydrolysis Tube, 6 mL is a high-quality glass tube designed for heated, acid-based, vacuum hydrolysis of proteins and peptides for amino acid analysis.

Features of Vacuum Hydrolysis Tube, 6 mL:

• Saves 10-18 hours on hydrolysis procedures when combined with 6N HCl (Part No. 24308)
• Provides for hydrolysis in 6 hours when heated at 150°C in a dry block heater (Note: the upper temperature limit of Vacuum Hydrolysis Tubes is 260°C in a block heater but only 110°C in an oven)
• Optimal hydrolysis control when used with Reacti-Therm Dry Block Heating System
• Compatible-fitting are available for use with Thermo Scientific Reacti-Therm Heating Modules

Applications of Vacuum Hydrolysis Tube:
• Protein/peptide hydrolysis
• Sample concentration
• Lyophilization
• Hydrazinolysis

Vacuum Hydrolysis Tubes are precision-designed for use in protein hydrolysis and subsequent analysis of protein and peptide amino acid compositions. The heavy-walled borosilicate glass construction ensures complete compatibility with all commonly used acid hydrolysis reagents. Acid hydrolysis at elevated temperatures, in vacuo, will hydrolyze most proteins and peptides to their amino acid components without significant decomposition.

Vacuum hydrolysis methods commonly use 6N constant boiling hydrochloric acid (HCl), a mixture of HCl and propionic acid, 3N mercaptoethanesulfonic acid or 4N methanesulfonic acid. The constant boiling HCl method uses standard protein hydrolysis at 105 to 110°C for 16 to 24 hours. Using the HCl/propionic acid method, hydrolysis times can be shortened to 15 minutes at 160°C. Tryptophan is easily degraded by acid hydrolysis in 6N HCl. The presence of oxygen, heavy metals and carbohydrates are contributing factors to this degradation. A 22-hour hydrolysis method was developed to improve tryptophan recovery from protein hydrolysis samples by using 3N mercaptoethanesulfonic acid. A second method to improve tryptophan recovery involves hydrolysis at 115°C using 4N methanesulfonic acid.

Related Products
Vacuum Hydrolysis Tube, 1 mL
Vacuum Hydrolysis Tube, 18 mL

Samco™ 16 × 125mm Disposable Culture Tubes (DCTs) (Thermo Scientific™)

Choose the material that suits your needs with these disposable culture tubes, in clarified polypropylene for strength and resistance, or polystyrene for superior clarity.

Low Protein Binding Collection Tubes (2.0 mL) (Thermo Scientific™)

Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (2.0 mL) help minimize protein sample loss during sample manipulation and are ideal in applications where processing steps are extensive.

Durable—able to tolerate centrifuge speeds up to 20,000 RCF
Compatible—suitable for IP and mass spectrometry applications
Nuclease-free—certified RNase/DNase-free

The Pierce Low Protein Binding Microcentrifuge Tubes help minimize sample loss in mass spectrometry and immunoprecipitation/co-immunoprecipitation applications. The polypropylene tubes are certified as DNase/RNase-free and are conveniently packaged (250 tubes per package) to minimize contamination.

Nunc™ Cell Culture Tubes (Thermo Scientific™)

Perform adherent cell cultures in biotechnology, pharmaceutical and research laboratories with Thermo Scientific™ Nunc™ Cell Culture Tubes.

Sterilin™ Round Base Rimless Borosilicate Glass Rimless Tubes (Thermo Scientific™)

Trust these round-base borosilicate glass tubes in high temperature, aggressive chemical applications. Ideal for use with samples that are sensitive to plastic tube leachables.

Sterilin™ Round Base Soda Glass Tubes (Thermo Scientific™)

Rely on these round base glass tubes for excellent chemical resistance for samples to 2mL or 4mL. Test tube profile for streamlined handling and excellent mixing capability.

Vacuum Hydrolysis Tube, 18 mL (Thermo Scientific™)

Thermo Scientific Pierce Vacuum Hydrolysis Tube, 18 mL is a high-quality glass tube designed for heated, acid-based, vacuum hydrolysis of proteins and peptides for amino acid analysis.

Features of Vacuum Hydrolysis Tube, 18 mL:

• Saves 10-18 hours on hydrolysis procedures when combined with 6N HCl (Part No. 24308)
• Provides for hydrolysis in 6 hours when heated at 150°C in a dry block heater (Note: the upper temperature limit of Vacuum Hydrolysis Tubes is 260°C in a block heater but only 110°C in an oven)
• Optimal hydrolysis control when used with Reacti-Therm Dry Block Heating System
• Compatible-fitting are available for use with Thermo Scientific Reacti-Therm Heating Modules

Applications of Vacuum Hydrolysis Tube:
• Protein/peptide hydrolysis
• Sample concentration
• Lyophilization
• Hydrazinolysis

Vacuum Hydrolysis Tubes are precision-designed for use in protein hydrolysis and subsequent analysis of protein and peptide amino acid compositions. The heavy-walled borosilicate glass construction ensures complete compatibility with all commonly used acid hydrolysis reagents. Acid hydrolysis at elevated temperatures, in vacuo, will hydrolyze most proteins and peptides to their amino acid components without significant decomposition.

Vacuum hydrolysis methods commonly use 6N constant boiling hydrochloric acid (HCl), a mixture of HCl and propionic acid, 3N mercaptoethanesulfonic acid or 4N methanesulfonic acid. The constant boiling HCl method uses standard protein hydrolysis at 105 to 110°C for 16 to 24 hours. Using the HCl/propionic acid method, hydrolysis times can be shortened to 15 minutes at 160°C. Tryptophan is easily degraded by acid hydrolysis in 6N HCl. The presence of oxygen, heavy metals and carbohydrates are contributing factors to this degradation. A 22-hour hydrolysis method was developed to improve tryptophan recovery from protein hydrolysis samples by using 3N mercaptoethanesulfonic acid. A second method to improve tryptophan recovery involves hydrolysis at 115°C using 4N methanesulfonic acid.

Related Products
Vacuum Hydrolysis Tube, 1 mL
Vacuum Hydrolysis Tube, 6 mL