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PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa (Thermo Scientific™)

Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine blue-, orange-, and green-stained proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots

PageRuler™ Unstained High Range Protein Ladder (Thermo Scientific™)

Thermo Scientific PageRuler Unstained High Range Protein Ladder is a mixture of eight (8) unstained proteins (60 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Features of PageRuler Unstained High Range Protein Ladder:

Size range—8 proteins spanning 60 to 250kDa
Ready-to-use—supplied in a loading buffer for direct loading on gels
Sharp bands—excellent accuracy for detection in with coomassie or silver stains
Reference band—150kDa band has greater intensity for easy orientation
Tagged—each protein in the ladder contains an integral Strep-tag™ II Sequence
Quality tested—each lot evaluated by SDS-PAGE and Western blotting

The protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 150kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained High Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.

Includes:
• Proteins (0.02 to 0.05 mg/mL of each) in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol

Applications:
• Accurate protein sizing on SDS-polyacrylamide gels and Western blots.

PageRuler™ Unstained Protein Ladder (Thermo Scientific™)

Thermo Scientific PageRuler Unstained Protein Ladder is a mixture of 14 recombinant, highly purified, unstained proteins (10 to 200 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Product features
• Reference bands—50 kDa band is stronger than the others for easy orientation
• Tagged—the ladder proteins contains an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II Sequence

Applications
• Accurate protein sizing on SDS-polyacrylamide gels and western blots

PageRuler™ Unstained Broad Range Protein Ladder (Thermo Scientific™)

Thermo Scientific PageRuler Unstained Broad Range Protein Ladder is a mixture of 11 proteins (5 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Product features
• Reference bands—the 100, 50, and 20 kDa bands are more intense for easy orientation
• Tagged— each protein in the contains an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequence

Applications
• Accurate protein sizing on SDS-polyacrylamide gels and western blots

PageRuler™ Prestained NIR Protein Ladder (Thermo Scientific™)

Thermo Scientific PageRuler Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250 kDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing following electrophoresis. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Product features
• Fluorescent—detect using 670 nm red laser or 700 nm channels with fluorescence imagers
• Reference band—55 kDa band has greater intensity for easy orientation

Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on NIR fluorescent SDS-polyacrylamide gels and western blots

PageRuler™ Prestained Protein Ladder, 10 to 180 kDa (Thermo Scientific™)

Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Compare and view all other protein standards and ladders ›

Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots

PageRuler™ Unstained Low Range Protein Ladder (Thermo Scientific™)

Thermo Scientific PageRuler Unstained Low Range Protein Ladder is a mixture of seven purified proteins and peptides (3.4 to 100 kDa) for use as size standards in peptide and small-protein gel electrophoresis. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Product features
• Reference bands—25 kDa band is stronger than the others for easy orientation
• Tagged—the ladder proteins (except for the 5 and 3.4 kDa peptides) contains an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates

Applications
• Accurate protein sizing on SDS-polyacrylamide gels and western blots

Spectra™ Multicolor High Range Protein Ladder (Thermo Scientific™)

Thermo Scientific Spectra Multicolor High Range Protein Ladder is a mixture of eight blue-, green-, and orange-stained proteins (40 to 300 kDa) for use as size standards for high-MW proteins in gel electrophoresis and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring of protein migration during SDS-PAGE
• Verifying western transfer efficiency
• Approximate sizing of proteins on SDS-polyacrylamide gels and western blots
• Locating a protein of interest for excision from an unstained preparative gel

BenchMark™ Protein Ladder (Invitrogen™)

BenchMark Protein Ladder consists of 15 unstained recombinant proteins ranging in molecular weight from 10 to 220 kDa. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Sizing of proteins on SDS-polyacrylamide gels and western blots

Pierce™ Prestained Protein MW Marker (Thermo Scientific™)

Thermo Scientific Prestained Protein Molecular Weight Marker is a mixture of six blue-stained natural proteins (20 to 120 kDa) for use as size standards in protein electrophoresis (SDS-PAGE). The protein standards are supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Component proteins: beta-galactosidase (120 kDa), bovine serum albumin (85 kDa), ovalbumin (50 kDa), cardonic anhydrase (35 kDa), beta-lactoglobulin (25 kDa), and lysozyme (20 kDa)

Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis.
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots

Pierce™ Unstained Protein MW Marker (Thermo Scientific™)

Thermo Scientific Unstained Protein Molecular Weight Marker is a mixture of seven native proteins (14.4 to 116 kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

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Applications
• Accurate protein sizing on SDS-polyacrylamide gels and western blots

Spectra™ Multicolor Low Range Protein Ladder (Thermo Scientific™)

Thermo Scientific Spectra Multicolor Low Range Protein Ladder is a mixture of six proteins and synthetic peptides (1.7 to 40 kDa) for use as size standards for peptides and small proteins in gel electrophoresis and western blotting. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Compare and view all other protein standards and ladders ›

Applications
• Monitoring of protein migration during SDS-PAGE
• Verifying western transfer efficiency
• Approximate sizing of proteins on SDS-polyacrylamide gels and western blots
• Locating a protein of interest for excision from an unstained preparative gel

Spectra™ Multicolor Broad Range Protein Ladder (Thermo Scientific™)

Thermo Scientific Spectra Multicolor Broad Range Protein Ladder is a 4-color (blue, orange, green, pink) protein standard containing 10 prestained proteins (10 to 260 kDa) for use in gel electrophoresis and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots

Novex™ Sharp Unstained Protein Standard (Invitrogen™)

Novex Sharp Unstained Protein Standard is designed for accurate and convenient molecular weight estimation of proteins during SDS-PAGE and western blotting. The standard consists of a wide range of molecular weight bands consisting of 12 proteins in the range of 3.5–260 kDa. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Accurate protein sizing on SDS-polyacrylamide gels and western blots