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PageRuler™ Unstained Protein Ladder (Thermo Scientific™)

Thermo Scientific PageRuler Unstained Protein Ladder is a mixture of 14 recombinant, highly purified, unstained proteins (10kDa to 200kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Features of PageRuler Unstained Protein Ladder:

Size range—14 proteins spanning 10 to 200kDa
Ready-to-use—supplied in a loading buffer for direct loading on gels
Sharp bands—excellent accuracy for detection in with coomassie or silver stains
Reference band—50kDa band is has greater intensity for easy orientation
Tagged—each protein in the ladder contains an integral Strep-tag™ II Sequence
Quality tested—each lot evaluated by SDS-PAGE and Western blotting

Each protein in the ladder contains an integral Strep-tag™ II Sequence which can be detected directly on Western blots using a Strep-Tactin™ Conjugates or an antibody against Strep-tag™ II Sequence (see Refs 2-3). The Ladder produces sharp bands on SDS-polyacrylamide gel following staining of the gel with Thermo Scientific Pierce Gel Stains or other coomassie dye or silver stains. The 50kDa protein band is of greater intensity and serves as a reference band.

Includes:
• Proteins (0.02 to 0.05 mg/mL of each) in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol

Applications:
• Accurate protein sizing on SDS-polyacrylamide gels and Western blots.

PageRuler™ Prestained Protein Ladder, 10 to 180 kDa (Thermo Scientific™)

Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of ten (10) blue-, orange- and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Features of PageRuler Prestained Protein Ladder:

Size range—10 proteins spanning 10 to 180 kDa
Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
Sharp bands—color-coded bands of similar intensity for easy visualization
Quality tested—each lot evaluated by SDS-PAGE and Western blotting
Two reference bands—orange at 70kDa and green at 10kDa
Membrane-compatible—colored bands transfer to membranes for Western blotting

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. The ladder contains one orange reference band at 70kDa and one green band at 10kDa.

Includes:
• Dye-stained proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol.

Applications:
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after Western blotting
• Sizing of proteins on SDS-polyacrylamide gels and Western blots

PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa (Thermo Scientific™)

Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine (9) blue-, orange- and green-stained proteins (10 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Features of PageRuler Plus Prestained Protein Ladder:

Size range—nine proteins spanning 10 to 250kDa
Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
Sharp bands—color-coded bands of similar intesity for easy visualization
Quality tested—each lot evaluated by SDS-PAGE and Western blotting
Bright reference bands—orange at 70 and 25kDa, and green at 10kDa
Membrane-compatible—colored bands transfer to membranes for Western blotting

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. A blue chromophore is bound to all proteins, except proteins of two reference bands of 70kDa and 25kDa that are colored with an orange dye and one green reference band of 10kDa. PageRuler Plus Prestained Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required before loading onto a gel.

Includes:
• Dye-stained proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol.

Applications:
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after Western blotting
• Sizing of proteins on SDS-polyacrylamide gels and Western blots

PageRuler™ Prestained NIR Protein Ladder (Thermo Scientific™)

Thermo Scientific PageRuler Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250KDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing following electrophoresis.

Features of PageRuler Prestained NIR Protein Ladder:

Size range—10 proteins spanning 11 to 250kDa
Fluorescent—detect using 670nm red laser or 700nm channels with fluorescence imagers
Ready-to-use—supplied in a loading buffer for direct loading on gels
Sharp bands—excellent sizing for near-infrared visualization of protein gels and blots
Reference band—55kDa band has greater intensity for easy orientation
Quality tested—each lot evaluated by SDS-PAGE and Western blotting

The protein MW markers in this ladder resolve into sharp bands when analyzed by SDS-PAGE and are labeled with a fluorescent dye for visualization with instruments equipped for detection of near-infrared (NIR) fluorescence. The bands are also directly visible because the proteins are prestained blue. The 55kDa band is of greater intensity and serves as a reference band. The protein ladder is conveniently packaged and ready to use: no heating, dilution or addition of a reducing agent is required before use.

Includes:
• Proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Applications:
• Protein sizing on SDS-polyacrylamide gels and Western blots for visualization with near-infrared (NIR) fluorescence imagers

Requires:
Fluorescent imager capable of near-infrared fluorescence detection, such as certain Typhoon™ Imagers and the LI-COR Odyssey™ Infrared Imaging System

PageRuler™ Unstained Broad Range Protein Ladder (Thermo Scientific™)

Thermo Scientific PageRuler Unstained Broad Range Protein Ladder is a mixture of 11 proteins (5kDa to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Features of PageRuler Unstained Broad Range Protein Ladder:

Size range—eleven proteins spanning 5 to 250kDa
Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
Sharp bands—excellent accuracy for detection in with coomassie or silver stains
Reference bands—the 100, 50 and 20kDa bands are more intense for easy orientation
Tagged—each protein in the ladder contains an integral Strep-tag™ II Sequence
Quality tested—each lot evaluated by SDS-PAGE and Western blotting

The protein MW markers in this ladder resolve into clearly identifiable sharp bands ranging in size from 5kDa to 250kDa when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 100, 50 and 20kDa protein bands are of greater intensity and serve as reference bands. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins (except for the 5kDa peptide) contain an integral Strep-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained Broad Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.

Includes:
• Proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol

Applications:
• Accurate protein sizing on SDS-polyacrylamide gels and Western blots.

PageRuler™ Unstained High Range Protein Ladder (Thermo Scientific™)

Thermo Scientific PageRuler Unstained High Range Protein Ladder is a mixture of eight (8) unstained proteins (60 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Features of PageRuler Unstained High Range Protein Ladder:

Size range—8 proteins spanning 60 to 250kDa
Ready-to-use—supplied in a loading buffer for direct loading on gels
Sharp bands—excellent accuracy for detection in with coomassie or silver stains
Reference band—150kDa band has greater intensity for easy orientation
Tagged—each protein in the ladder contains an integral Strep-tag™ II Sequence
Quality tested—each lot evaluated by SDS-PAGE and Western blotting

The protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 150kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained High Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.

Includes:
• Proteins (0.02 to 0.05 mg/mL of each) in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol

Applications:
• Accurate protein sizing on SDS-polyacrylamide gels and Western blots.

PageRuler™ Unstained Low Range Protein Ladder (Thermo Scientific™)

Thermo Scientific PageRuler Unstained Low Range Protein Ladder is a mixture of seven (7) purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small-protein gel electrophoresis.

Features of PageRuler Unstained Low Range Protein Ladder:

Size range—seven proteins and one peptide spanning 3.4 to 100kDa
Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
Sharp bands—excellent accuracy for detection in with coomassie or silver stains
Reference bands—25kDa band is stronger than the others for easy orientation
Tagged—each protein in the ladder contains an integral Strep-tag™ II Sequence
Quality tested—each lot evaluated by SDS-PAGE and Western blotting

The protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 100kDa protein band indicates the approximate position of large proteins and the 25kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins (except for the 5 and 3.4kDa peptides) contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained Broad Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.

Includes:
• Proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol

Applications:
• Accurate protein sizing on SDS-polyacrylamide gels and Western blots.