Diameter (Metric) Inner


For Use With (Application)


For Use With (Equipment)


Hydrophobicity


Temperature (Metric)


Products

POROS™ 50 HS Strong Cation Exchange Resin Thermo Scientific™

Applied Biosystems™ POROS™ Strong Cation Exchange Media. POROS™ 50 micron bulk resins is available for low pressure applications, including large scale downstream bioprocessing.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

POROS™ XS Strong Cation Exchange Resin Thermo Scientific™

POROS™ XS resin is the first high-capacity, high-resolution resin that allows loading to more than 100 mg/mL capacity in the presence of up to 15 mS/cm NaCl, while delivering unprecedented separation capability.

A major improvement on traditional cation exchangers, POROS™ XS resin sets the standard for cation exchange chromatography.

• Dynamic binding capacity leads to reduced column size, smaller footprint, decreased buffer usage, and reduced cycling
• High resolution supports better product yield and improved impurity clearance
• Robust salt tolerance allows improved process flexibility, alignment, and process flow streamlining
• Low back pressure and linear pressure responses help improve scalability
• Rigid polymeric bead with covalent surface chemistry makes for easier handling and packing, and superior physical and chemical stability, resulting in a robust manufacturing process

Dynamic Binding Capacity
POROS™ XS resin has a high binding capacity of more than 100 mg/mL, at 5% breakthrough, over a broad range of process conditions. Figure 1 demonstrates unparalleled binding capacity across a broad range of pH and salt conditions. Higher dynamic binding capacity was achieved by optimizing the base bead pore structure and surface chemistry.

High Resolution
Using an optimized 50 µm particle size, POROS™ XS resin maintains the superior resolving capacity of POROS™ HS resin (Figure 2) while overcoming the challenging impurity levels associated with high titer processes. Resolution is consistent across a wide range of operating flow rates (Figure 3) and maintained independent of protein load (Figure 4).

Robust Salt Tolerance
POROS™ XS resin has high and consistent protein capacity across a broad range of salt concentrations (Figure 5) up to 150 mM NaCl (15 mS/cm conductivity). The ability to load columns under higher conductivity reduces or helps eliminate the dilution of column feed streams or the removal of unit operations, such as tangential flow filtration. The optimized surface ionic capacity of POROS™ XS resin minimizes competitive binding between the protein of interest and the counterion, resulting in superior salt tolerance.

POROS™ A 20 µm Column, 2.1 x 30 mm, 0.1 mL Thermo Scientific™

Applied Biosystems™ POROS™ Prepacked Protein A, Affinity Column. POROS™ Protein A is based on an immobilized recombinant Protein A functional group designed for high throughput purification of antibodies.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High Capacity, High Resolution, High Speed

In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

POROS™ 50 HQ Strong Anion Exchange Resin Thermo Scientific™

Applied Biosystems™ POROS™ Strong Anion Exchange Media. POROS™ 50 micron bulk resins is available for low pressure applications, including large scale downstream bioprocessing. POROS™ HQ is based on a quaternized polyethyleneimine functional group yielding a high capacity, High Performance Chromatography™ resins designed for the separation and purification of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.
• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

POROS™ A 20 µm Column, Stainless Steel, 2.1 x 30 mm, 0.1 mL Thermo Scientific™

Applied Biosystems™ POROS™ Prepacked Protein A, Affinity Column. POROS™ Protein A is based on an immobilized recombinant Protein A functional group designed for high throughput purification of antibodies.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High Capacity, High Resolution, High Speed

In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

POROS™ R1 20 µm Column, Stainless Steel, 2.1 x 30 mm, 0.1 mL Thermo Scientific™

Applied Biosystems™ POROS™ Prepacked Reversed-Phase Column. POROS™ R1 is a 4000 angstrom pore size Poly[styrenevinylbenzene] particle, suitable for the reversed-phase separation of biomolecules.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

POROS™ A 20 µm Column, 4.6 x 50 mm, 0.8 mL Thermo Scientific™

Applied Biosystems™ POROS™ Prepacked Protein A, Affinity Column. POROS™ Protein A is based on an immobilized recombinant Protein A functional group designed for high throughput purification of antibodies.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High Capacity, High Resolution, High Speed

In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

POROS™ HQ50 RoboColumn Thermo Scientific™

RoboColumn™ chromatography products are small columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms. These particular RoboColumns contain POROS™ HQ50 resin, a 50-micron strong anion-exchange resin suitable for low-pressure applications, including large-scale downstream bioprocessing.

POROS HQ is a high-performance chromatography resin based on a quaternized polyethyleneimine functional group, yielding a high capacity. It is designed for the separation and purification of biomolecules for analytical to process scale separations.

Features of the resin:
• Higher productivity—through high throughput and high dynamic capacity associated with high performance chromatography
• Chemical stability—allows aggressive cleaning and sanitization
• Enhanced biomolecule access—provided via large pores (500-10000 Å)
• Polystyrenedivinylbenzene particles—yield a robust, easily packable matrix
• Better separation methods in less time—the speed of high performance chromatography reduces weeks of experimentation to only a few hours, leaving more time to explore all the variables of your separation

RoboColumn is a registered trademark of ATOLL GmbH.

POROS™ XQ RoboColumn Thermo Scientific™

RoboColumn™ chromatography products are small columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms. These particular RoboColumns contain POROS™ XQ resin, a 50-micron strong anion-exchange resin suitable for low-pressure applications, including large-scale downstream bioprocessing.

POROS XQ is a high-capacity, high-resolution, salt-tolerant resin that that enables >140 mg/mL dynamic binding capacity in the presence of up to 6.6 mS/cm NaCl, while delivering exceptional separation performance. A major improvement to the performance of traditional anion exchangers, POROS XQ resin sets the standard for strong anion exchange chromatography in both bind & elute or flow-through modes of operation.

Features of the resin:
• High dynamic binding capacity leading to reduced column size, smaller footprint, decreased water and buffer usage, and reduced cycling
• High resolution supporting improved product yield and purity, ultimately improving the cost of goods for the molecule being produced
• Robust salt tolerance allowing enhanced process design flexibility and streamlining of process flow
• Low operating back pressure and linear pressure versus flow responses driving flexible scalability
• Rigid polymeric bead with covalent surface chemistry for easier handling and packing, and superior physical and chemical stability enabling a more robust downstream purification process

RoboColumn is a registered trademark of ATOLL GmbH.

POROS™ A 20 µm Column, 4.6 x 100 mm, 1.7 mL Thermo Scientific™

Applied Biosystems™ POROS™ Prepacked Protein A, Affinity Column. POROS™ Protein A is based on an immobilized recombinant Protein A functional group designed for high throughput purification of antibodies.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High Capacity, High Resolution, High Speed

In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

POROS™ PI50 RoboColumn Thermo Scientific™

RoboColumn™ chromatography products are small columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms. These particular RoboColumns contain POROS™ PI50 resin, a 50-micron weak anion-exchange resin suitable for low-pressure applications, including large-scale downstream bioprocessing.

POROS PI50 chromatography resin is based on a polyethyleneimine functional group, yielding a high capacity. It is designed for the separation and purification of biomolecules.

Features of the resin:
• Higher productivity—through high throughput and high dynamic capacity associated with high performance chromatography
• Chemical stability—allows aggressive cleaning and sanitization
• Enhanced biomolecule access—provided via large pores (500-10000 Å)
• Polystyrenedivinylbenzene particles—yield a robust, easily packable matrix
• Better separation methods in less time—the speed of high performance chromatography reduces weeks of experimentation to only a few hours, leaving more time to explore all the variables of your separation

RoboColumn is a registered trademark of ATOLL GmbH.

POROS™ D50 RoboColumn Thermo Scientific™

RoboColumn™ chromatography products are small columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms. These particular RoboColumns contain POROS™ D50 resin, a 50-micron weak anion-exchange resin suitable for low-pressure applications, including large-scale downstream bioprocessing.

Features of the resin:
• Higher productivity—through high throughput and high dynamic capacity associated with high performance chromatography
• Chemical stability—allows aggressive cleaning and sanitization
• Enhanced biomolecule access—provided via large pores (500-10000 Å)
• Polystyrenedivinylbenzene particles—yield a robust, easily packable matrix
• Better separation methods in less time—the speed of high performance chromatography reduces weeks of experimentation to only a few hours, leaving more time to explore all the variables of your separation

RoboColumn is a registered trademark of ATOLL GmbH.

POROS™ CaptureSelect™ AAV8 Affinity Resin Thermo Scientific™

POROS™ CaptureSelect™ AAV8 high performance affinity resin is engineered to address the high selectivity and capacity requirements needed for the purification of viral vectors used for gene therapy applications and is specifically intended for the purification of adeno-associated virus type 8 (AAV8).

Features of POROS CaptureSelect AAV8 resin include:
• One-step AAV8 purification from crude material with high purity and yield
• High specificity and capacity, which helps reduce the process volume significantly for subsequent steps and maximizes yield
• Low operating back pressure and linear pressure versus flow responses driving flexible scalability
• Superior capacity maintained at high flow rates
• Rigid polymeric bead with covalent surface chemistry for easier handling and packing, with superior physical and chemical stability enabling a more robust downstream purification process
• Non-animal derived—the ligand is a 13-kDa single-domain fragment that comprises the 3 CDRs that form the antigen-binding domain and is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

POROS CaptureSelect AAV8 affinity resin specifically binds AAV8 and enables a robust, fast, and efficient purification process with excellent purity and yield obtained in one step. The use of this affinity resin will enable significant improvement to downstream processing by reducing the purification steps and maximizing productivity, for scalability and processing consistency.

Main characteristics:

Matrix: cross-linked poly(styrene-divinylbenzene)
Average particle size: 50 µm
Ligand: CaptureSelect AAV8 affinity ligand
Ligand coupling method: carbonyldiimidazole (CDI)
Binding capacity: >1x1013 genome copies/mL of resin
Elution conditions: acidic elution with 0.1 M glycine, pH 3.0
Mechanical resistance: 100 bar (1,450 psi; 10 MPa)
Formulation buffer: 18% (v/v) ethanol

POROS™ Benzyl Ultra RoboColumn Thermo Scientific™

This RoboColumn product is pre-packed with POROS Benzyl Ultra Hydrophobic Interaction Chromatography (HIC) Resin that is well suited for aggregate removal from monoclonal antibody monomer in flow-through mode at low salt concentrations in large scale downstream bioprocessing.

RoboColumn products are small chromatography columns that are provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms.Features of POROS Benzyl Ultra HIC Resin include:

• Superior resolution capability while maintaining excellent pressure-flow properties, achieved by combining the high-flow characteristics of a POROS base bead with a 50-micron particle size. Higher flow rates help improve productivity.
• Unique selectivity due to the novel hydrophobic ligand, allowing for purification of a wide range of biomolecules, including therapeutic proteins, antibody fragments, antibody drug conjugates, and enzymes
• High capacity, reducing the process volume significantly for subsequent steps
• A rigid polymeric bead with covalent surface chemistry for easier handling and packing with superior physical and chemical stability, allowing cleaning with various clean-in-place (CIP) solutions such as sodium hydroxide and enabling a more robust downstream purification process

These features of POROS Benzyl Ultra HIC Resin are a major improvement to the performance of traditional HIC resins, setting the standard for hydrophobic interaction chromatography.

RoboColumn is a registered trademark of ATOLL GmbH.

POROS™ HP2 20 µm Column, 4.6 x 50 mm, 0.8 mL Thermo Scientific™

Applied Biosystems™ POROS™ Prepacked Hydrophobic Interaction Chromatography Column. POROS™ HP2 is a high density phenyl, High Performance Chromatography™ resins suitable for the separation and purification of proteins.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.
Results per page
    spinner