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POROS™ PI50 RoboColumn (Thermo Scientific™)

RoboColumn™ chromatography products are small columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms. These particular RoboColumns contain POROS™ PI50 resin, a 50-micron weak anion-exchange resin suitable for low-pressure applications, including large-scale downstream bioprocessing.

POROS PI50 chromatography resin is based on a polyethyleneimine functional group, yielding a high capacity. It is designed for the separation and purification of biomolecules.

Features of the resin:
• Higher productivity—through high throughput and high dynamic capacity associated with high performance chromatography
• Chemical stability—allows aggressive cleaning and sanitization
• Enhanced biomolecule access—provided via large pores (500-10000 Å)
• Polystyrenedivinylbenzene particles—yield a robust, easily packable matrix
• Better separation methods in less time—the speed of high performance chromatography reduces weeks of experimentation to only a few hours, leaving more time to explore all the variables of your separation

RoboColumn is a registered trademark of ATOLL GmbH.

POROS™ Ethyl RoboColumn (Thermo Scientific™)

This RoboColumn product is pre-packed with POROS Ethyl Hydrophobic Interaction Chromatography (HIC) Resin and suitable for impurity removal during large-scale downstream purification of biomolecules, primarily for bind/elute operations.

RoboColumn products are small chromatography columns that are provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms.Features of POROS Ethyl HIC Resin include:

• Superior resolution capability while maintaining excellent pressure-flow properties, achieved by combining the high-flow characteristics of a POROS base bead with a 50-micron particle size. Higher flow rates help improve productivity.
• Unique selectivity due to the novel hydrophobic ligand, allowing for purification of a wide range of biomolecules, including therapeutic proteins, antibody fragments, antibody drug conjugates, and enzymes
• High capacity, reducing the process volume significantly for subsequent steps
• A rigid polymeric bead with covalent surface chemistry for easier handling and packing with superior physical and chemical stability, allowing cleaning with various clean-in-place (CIP) solutions such as sodium hydroxide and enabling a more robust downstream purification process

These features of POROS Ethyl HIC Resin are a major improvement to the performance of traditional HIC resins, setting the standard for hydrophobic interaction chromatography.

RoboColumn is a registered trademark of ATOLL GmbH.

POROS™ GoPure™ Benzyl Pre-packed Column, 0.5 x 5 cm, 1 mL (Thermo Scientific™)

POROS GoPure 0.5 cmD pre-packed columns are intended for the screening of POROS ion exchange and hydrophobic interaction chromatography (HIC) resins or for small-scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS high-performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS Benzyl HIC Resin in this GoPure column has an aromatic hydrophobic benzyl ligand, suited for hydrophobic interaction chromatography (HIC) for both bind/elute and flow-through purifications.

• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the POROS GoPure family of pre-packed columns simplifies process scale-up and process modeling

POROS GoPure 0.5 cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS resin-screening platform. The 1 mL bed volume reduces sample requirements, and the recommended > 3 min residence time (0.3 mL/min) on this 5-cm-length column equates to 400 cm/hr on a 20-cm bed height. Detailed specifications may be found in the POROS GoPure 0.5 cmD Operating Instructions below.

Other POROS hydrophobic interaction and ion exchange resins are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, POROS GoPure pre-packed columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 mL to 22 mL.

POROS™ D50 RoboColumn (Thermo Scientific™)

RoboColumn™ chromatography products are small columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms. These particular RoboColumns contain POROS™ D50 resin, a 50-micron weak anion-exchange resin suitable for low-pressure applications, including large-scale downstream bioprocessing.

Features of the resin:
• Higher productivity—through high throughput and high dynamic capacity associated with high performance chromatography
• Chemical stability—allows aggressive cleaning and sanitization
• Enhanced biomolecule access—provided via large pores (500-10000 Å)
• Polystyrenedivinylbenzene particles—yield a robust, easily packable matrix
• Better separation methods in less time—the speed of high performance chromatography reduces weeks of experimentation to only a few hours, leaving more time to explore all the variables of your separation

RoboColumn is a registered trademark of ATOLL GmbH.

POROS™ XS RoboColumn (Thermo Scientific™)

RoboColumn™ chromatography products are small columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms. These particular RoboColumns contain POROS™ XS resin, a 50-micron strong cation-exchange resin suitable for low-pressure applications, including large-scale downstream bioprocessing.

POROS XS resin is the first high-capacity, high-resolution resin that allows loading to more than 100 mg/mL capacity in the presence of up to 15 mS/cm NaCl, while delivering unprecedented separation capability. A major improvement on traditional cation exchangers, POROS XS resin sets the standard for cation exchange chromatography.

Features of the resin:
• Dynamic binding capacity leads to reduced column size, smaller footprint, decreased buffer usage, and reduced cycling
• High resolution supports better product yield and improved impurity clearance
• Robust salt tolerance allows improved process flexibility, alignment, and process flow streamlining
• Low back pressure and linear pressure responses help improve scalability
• Rigid polymeric bead with covalent surface chemistry makes for easier handling and packing, and superior physical and chemical stability, resulting in a robust manufacturing process

RoboColumn is a registered trademark of ATOLL GmbH.

POROS™ MabCapture™ A Select RoboColumn (Thermo Scientific™)

RoboColumn™ chromatography products are small columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms. These particular RoboColumns contain POROS™ MabCapture™ A Select affinity resin, which is suitable for monoclonal antibody large scale downstream bioprocessing.

POROS MabCapture A Select affinity resin is a the latest high-throughput, lower-priced Protein A chromatography resin, combining past improvements to MabCapture A with our own Protein A ligand, enabling the same high-throughput purification performance at a significant reduction in price over most Protein A chromatography resins . These advances provide a monoclonal antibody purification resin that demonstrates high dynamic binding performance at low flow rates and maintains superior binding capacity at high linear velocities where other chromatography resins products cannot operate.

Features of the resin:
• Obtain the highest dynamic binding capacity for monoclonal antibodies vs. conventional agarose or silica based Protein A medias
• Maintain high dynamic binding capacity at flow rates over 700 cm/hr
• Design processes with greater flexibility (shorter bed heights, faster flow rates or both) due to the improvements made to the antibody binding efficiency
• Clean and sanitize with standard sodium hydroxide agents utilized for other chromatography products
• Recognize major increases to process productivity and reduce the downstream bottleneck

RoboColumn is a registered trademark of ATOLL GmbH.

POROS™ Benzyl Ultra RoboColumn (Thermo Scientific™)

This RoboColumn product is pre-packed with POROS Benzyl Ultra Hydrophobic Interaction Chromatography (HIC) Resin that is well suited for aggregate removal from monoclonal antibody monomer in flow-through mode at low salt concentrations in large scale downstream bioprocessing.

RoboColumn products are small chromatography columns that are provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms.Features of POROS Benzyl Ultra HIC Resin include:

• Superior resolution capability while maintaining excellent pressure-flow properties, achieved by combining the high-flow characteristics of a POROS base bead with a 50-micron particle size. Higher flow rates help improve productivity.
• Unique selectivity due to the novel hydrophobic ligand, allowing for purification of a wide range of biomolecules, including therapeutic proteins, antibody fragments, antibody drug conjugates, and enzymes
• High capacity, reducing the process volume significantly for subsequent steps
• A rigid polymeric bead with covalent surface chemistry for easier handling and packing with superior physical and chemical stability, allowing cleaning with various clean-in-place (CIP) solutions such as sodium hydroxide and enabling a more robust downstream purification process

These features of POROS Benzyl Ultra HIC Resin are a major improvement to the performance of traditional HIC resins, setting the standard for hydrophobic interaction chromatography.

RoboColumn is a registered trademark of ATOLL GmbH.

POROS™ Benzyl RoboColumn (Thermo Scientific™)

This RoboColumn product is pre-packed with POROS Benzyl Hydrophobic Interaction Chromatography (HIC) Resin that is suitable for impurity removal in the large scale downstream purification of biomolecules, for both bind/elute and flow-through operations.

RoboColumn products are small chromatography columns that are provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms.Features of POROS Benzyl HIC Resin include:

• Superior resolution capability while maintaining excellent pressure-flow properties, achieved by combining the high-flow characteristics of a POROS base bead with a 50-micron particle size. Higher flow rates help improve productivity.
• Unique selectivity due to the novel hydrophobic ligand, allowing for purification of a wide range of biomolecules, including therapeutic proteins, antibody fragments, antibody drug conjugates, and enzymes
• High capacity, reducing the process volume significantly for subsequent steps
• A rigid polymeric bead with covalent surface chemistry for easier handling and packing with superior physical and chemical stability, allowing cleaning with various clean-in-place (CIP) solutions such as sodium hydroxide and enabling a more robust downstream purification process

These features of POROS Benzyl HIC Resin are a major improvement to the performance of traditional HIC resins, setting the standard for hydrophobic interaction chromatography.

RoboColumn is a registered trademark of ATOLL GmbH.

POROS™ GoPure™ XQ Pre-packed Column, 0.5 x 5 cm, 1 mL (Thermo Scientific™)

POROS™ GoPure™ 0.5 cm diameter x 5 cm long prepacked columns are intended for screening of POROS™ ion exchange resins or for small scale purification. These prepacked columns are ready to connect and have the following features:
• Dimensions of 0.5 cmD x 5 cmL with a nominal 1 mL bed volume that provides a uniform screening platform
• Direct connections to standard chromatography systems
• Packed with POROS™ high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS™ XQ resin in this GoPure™ column features a strong anion exchange functional group and is a high capacity and high resolution resin with excellent salt tolerance.

• Convenient—ready-to-run columns simplify and speed POROS™ resin evaluations
• Conserves sample—GoPure™ 0.5 cmD x 5 cmL columns, with their 1 mL nominal bed volume, minimize the sample needed for screening multiple resins
• Simplified scalability—reproducible GoPure™ pre-packed columns simplify process scale up or process modeling

GoPure™ 0.5 cmD x 5 cmL prepacked columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS™ resin screening platform. The 1 mL bed volume reduces sample requirements, and a recommended ≥3 minute residence time (0.3 mL/min) on this 5 cm length column equates to 400 cm/hr on a 20 cm bed height.
Other POROS™ anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS™ high performance chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scale-up, POROS™ GoPure™ pre-packed columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 mL to 6.3 L.

POROS™ GoPure™ Benzyl Ultra Pre-packed Column, 0.5 x 5 cm, 1 mL (Thermo Scientific™)

POROS GoPure 0.5 cmD pre-packed columns are intended for the screening of POROS ion exchange and hydrophobic interaction chromatography (HIC) resins or for small-scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS high-performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS Benzyl Ultra HIC Resin in this GoPure column has an aromatic hydrophobic benzyl ligand, well suited for hydrophobic interaction chromatography (HIC) for aggregate removal from monoclonal antibody monomer in flow-through mode at low salt concentrations.

• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the POROS GoPure family of pre-packed columns simplifies process scale-up and process modeling

POROS GoPure 0.5 cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS resin-screening platform. The 1 mL bed volume reduces sample requirements, and the recommended > 3 min residence time (0.3 mL/min) on this 5-cm-length column equates to 400 cm/hr on a 20-cm bed height. Detailed specifications may be found in the POROS GoPure 0.5 cmD Operating Instructions below.

Other POROS hydrophobic interaction and ion exchange resins are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, POROS GoPure pre-packed columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 mL to 22 mL.

POROS™ HS50 RoboColumn (Thermo Scientific™)

RoboColumn™ chromatography products are small columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms. These particular RoboColumns contain POROS™ HS50 resin, a 50-micron strong cation-exchange resin suitable for low-pressure applications, including large-scale downstream bioprocessing.

Features of the resin:
• Higher productivity—through high throughput and high dynamic capacity associated with high performance chromatography
• Chemical stability—allows aggressive cleaning and sanitization
• Enhanced biomolecule access—provided via large pores (500-10000 Å)
• Polystyrenedivinylbenzene particles—yield a robust, easily packable matrix
• Better separation methods in less time—the speed of high performance chromatography reduces weeks of experimentation to only a few hours, leaving more time to explore all the variables of your separation

RoboColumn is a registered trademark of ATOLL GmbH.

POROS™ HQ50 RoboColumn (Thermo Scientific™)

RoboColumn™ chromatography products are small columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms. These particular RoboColumns contain POROS™ HQ50 resin, a 50-micron strong anion-exchange resin suitable for low-pressure applications, including large-scale downstream bioprocessing.

POROS HQ is a high-performance chromatography resin based on a quaternized polyethyleneimine functional group, yielding a high capacity. It is designed for the separation and purification of biomolecules for analytical to process scale separations.

Features of the resin:
• Higher productivity—through high throughput and high dynamic capacity associated with high performance chromatography
• Chemical stability—allows aggressive cleaning and sanitization
• Enhanced biomolecule access—provided via large pores (500-10000 Å)
• Polystyrenedivinylbenzene particles—yield a robust, easily packable matrix
• Better separation methods in less time—the speed of high performance chromatography reduces weeks of experimentation to only a few hours, leaving more time to explore all the variables of your separation

RoboColumn is a registered trademark of ATOLL GmbH.

POROS™ XQ RoboColumn (Thermo Scientific™)

RoboColumn™ chromatography products are small columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms. These particular RoboColumns contain POROS™ XQ resin, a 50-micron strong anion-exchange resin suitable for low-pressure applications, including large-scale downstream bioprocessing.

POROS XQ is a high-capacity, high-resolution, salt-tolerant resin that that enables >140 mg/mL dynamic binding capacity in the presence of up to 6.6 mS/cm NaCl, while delivering exceptional separation performance. A major improvement to the performance of traditional anion exchangers, POROS XQ resin sets the standard for strong anion exchange chromatography in both bind & elute or flow-through modes of operation.

Features of the resin:
• High dynamic binding capacity leading to reduced column size, smaller footprint, decreased water and buffer usage, and reduced cycling
• High resolution supporting improved product yield and purity, ultimately improving the cost of goods for the molecule being produced
• Robust salt tolerance allowing enhanced process design flexibility and streamlining of process flow
• Low operating back pressure and linear pressure versus flow responses driving flexible scalability
• Rigid polymeric bead with covalent surface chemistry for easier handling and packing, and superior physical and chemical stability enabling a more robust downstream purification process

RoboColumn is a registered trademark of ATOLL GmbH.

POROS™ GoPure™ Ethyl Pre-packed Column, 0.5 x 5 cm, 1 mL (Thermo Scientific™)

POROS GoPure 0.5 cmD pre-packed columns are intended for the screening of POROS ion exchange and hydrophobic interaction chromatography (HIC) resins or for small-scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS high-performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS Ethyl HIC Resin in this GoPure column has a novel hydrophobic ethyl ligand, making it suited primarily for bind/elute purifications.

• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the POROS GoPure family of pre-packed columns simplifies process scale-up and process modeling

POROS GoPure 0.5 cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS resin-screening platform. The 1 mL bed volume reduces sample requirements, and the recommended > 3 min residence time (0.3 mL/min) on this 5-cm-length column equates to 400 cm/hr on a 20-cm bed height. Detailed specifications may be found in the POROS GoPure 0.5 cmD Operating Instructions below.

Other POROS hydrophobic interaction and ion exchange resins are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, POROS GoPure pre-packed columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 mL to 22 mL.

POROS™ MabCapture™ A RoboColumn (Thermo Scientific™)

RoboColumn™ chromatography products are small columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms. These particular RoboColumns contain POROS™ MabCapture™ A affinity resin, which is suitable for monoclonal antibody large-scale downstream bioprocessing.

POROS MabCapture A is a 50-micron affinity resin that combines improvements made in bead and chemistry technology. These advances provide a monoclonal antibody purification resin that not only demonstrates the highest dynamic binding performance at low flow rates, but also one that can maintain superior binding capacity at high linear velocities where other chromatography resins products cannot operate.

Features of the resin:
• Obtain the highest dynamic binding capacity for monoclonal antibodies vs. conventional agarose or silica based Protein A medias
• Maintain high dynamic binding capacity at flow rates over 700 cm/hr
• Design processes with greater flexibility (shorter bed heights, faster flow rates or both) due to the improvements made to the antibody binding efficiency
• Clean and sanitize with standard sodium hydroxide agents utilized for other chromatography products

RoboColumn is a registered trademark of ATOLL GmbH.