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POROS™ 50 HQ Strong Anion Exchange Resin (Thermo Scientific™)

Applied Biosystems™ POROS™ Strong Anion Exchange Media. POROS™ 50 micron bulk resins is available for low pressure applications, including large scale downstream bioprocessingg. POROS™ HQ is based on a quaternized polyethyleneimine functional group yielding a high capacity, High Performance Chromatography™ resins designed for the separation and purification of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.
• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

POROS™ 20 HQ Strong Anion Exchange Resin (Thermo Scientific™)

Applied Biosystems™ POROS™ Strong Anion Exchange Media for scale-up of separations developed with POROS 20 micron Self Pack™ media. POROS™ HQ is based on a quaternized polyethyleneimine functional group yielding a high capacity, High Performance Chromatography™ resins designed for the separation and purification of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

POROS™ HQ 20 µm Column, 4.6 x 100 mm, 1.7 mL (Thermo Scientific™)

Applied Biosystems™ POROS™ Prepacked Strong Anion Exchange Column. POROS™ HQ is based on a quaternized polyethyleneimine functional group yielding a high capacity, High Performance Chromatography™ resins designed for the separation and purification of biomolecules.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

POROS™ HQ 10 µm Column, 2.1 x 100 mm, 0.3 mL (Thermo Scientific™)

Applied Biosystems™ POROS™ Prepacked Strong Anion Exchange Column. POROS™ HQ is based on a quarternized polyethyleneimine functional group yielding a high capacity, High Performance Chromatography™ resins designed for the separation and purification of biomolecules.
10 micron particle size is used for the highest resolution chromatography, and is suitable for analytical to small scale preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

POROS™ HQ 10 µm Column, 4.6 x 50 mm, 0.8 mL (Thermo Scientific™)

Applied Biosystems™ POROS™ Prepacked Strong Anion Exchange Column. POROS™ HQ is based on a quarternized polyethyleneimine functional group yielding a high capacity, High Performance Chromatography™ resins designed for the separation and purification of biomolecules.
10 micron particle size is used for the highest resolution chromatography, and is suitable for analytical to small scale preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

POROS™ HQ 10 µm Column, 4.6 x 100 mm, 1.7 mL (Thermo Scientific™)

Applied Biosystems™ POROS™ Prepacked Strong Anion Exchange Column. POROS™ HQ is based on a quarternized polyethyleneimine functional group yielding a high capacity, High Performance Chromatography™ resins designed for the separation and purification of biomolecules.
10 micron particle size is used for the highest resolution chromatography, and is suitable for analytical to small scale preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

POROS™ HQ 20 µm Column, 4.6 x 50 mm, 0.8 mL (Thermo Scientific™)

Applied Biosystems™ POROS™ Prepacked Strong Anion Exchange Column. POROS™ HQ is based on a quaternized polyethyleneimine functional group yielding a high capacity, High Performance Chromatography™ resins designed for the separation and purification of biomolecules.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

POROS™ HQ 20 µm Column, 2.1 x 30 mm, 0.1 mL (Thermo Scientific™)

Applied Biosystems™ POROS™ Prepacked Strong Anion Exchange Column. POROS™ HQ is based on a quaternized polyethyleneimine functional group yielding a high capacity, High Performance Chromatography™ resins designed for the separation and purification of biomolecules.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

POROS™ GoPure™ HQ Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS HQ 50 µm resin in this GoPure column has a quaternized polyethyleneimine functional group for strong anion exchange chromatography.

Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

POROS™ GoPure™ HQ Pre-packed Column, 0.5 x 5 cm, 1 mL (Thermo Scientific™)

GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS™ ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS™ high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The resin in this GoPure™ HQ 50 µm Column has a quaternized polyethyleneimine functional group for strong anion exchange chromatography.

• Convenient—ready-to-run columns simplify and speed POROS™ resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the GoPure™ family of pre-packed columns simplifies process scale-up and process modeling

GoPure™ 0.5 cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS™ resin-screening platform. The 1 mL bed volume reduces sample requirements, and the recommended > 3 min residence time (0.3 ml/min) on this 5 cm length column equates to 400 cm/hr on a 20 cm bed height. Detailed specifications may be found in the GoPure 0.5 cmD Operating Instructions below.

Other POROS™ anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS™ High Performance Chromatography™ resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, GoPure™ Pre-Packed Columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 ml to 6.3 liters.