Products

POROS™ GoPure™ HS Pre-packed Column, 0.5 x 5 cm, 1 mL Thermo Scientific™

GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS™ ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS™ high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The resin in this GoPure™ HS 50 µm Column has a sulphopropyl functional group for strong cation exchange chromatography.

• Convenient—ready-to-run columns simplify and speed POROS™ resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the GoPure™ family of pre-packed columns simplifies process scale-up and process modeling

GoPure™ 0.5 cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS™ resin-screening platform. The 1 mL bed volume reduces sample requirements, and the recommended > 3 min residence time (0.3 ml/min) on this 5 cm length column equates to 400 cm/hr on a 20 cm bed height. Detailed specifications may be found in the GoPure 0.5 cmD Operating Instructions below.

Other POROS™ anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS™ High Performance Chromatography™ resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, GoPure™ Pre-Packed Columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 ml to 6.3 liters.

POROS™ GoPure™ XS Pre-packed Column, 0.5 x 5 cm, 1 mL Thermo Scientific™

GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS™ ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS™ high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The resin in this GoPure™ XS Column has a sulphopropyl functional group for strong cation exchange chromatography.

• Convenient—ready-to-run columns simplify and speed POROS™ resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the GoPure™ family of pre-packed columns simplifies process scale-up and process modeling

GoPure™ 0.5 cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS™ resin-screening platform. The 1 mL bed volume reduces sample requirements, and the recommended > 3 min residence time (0.3 ml/min) on this 5 cm length column equates to 400 cm/hr on a 20 cm bed height. Detailed specifications may be found in the GoPure 0.5 cmD Operating Instructions below.

Other POROS™ anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS™ High Performance Chromatography™ resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, GoPure™ Pre-Packed Columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 ml to 6.3 liters.

POROS™ XS Strong Cation Exchange Resin Thermo Scientific™

POROS™ XS Strong Cation Exchange Resin is the first high-capacity, high-resolution resin that allows loading to more than 100 mg/mL capacity in the presence of up to 15 mS/cm NaCl, while delivering unprecedented separation capability. A major improvement on traditional cation exchangers, POROS XS resin sets the standard for cation exchange chromatography.

• Dynamic binding capacity leads to reduced column size, smaller footprint, decreased buffer usage, and reduced cycling
• High resolution supports better product yield and improved impurity clearance
• Robust salt tolerance allows improved process flexibility, alignment, and process flow streamlining
• Low back pressure and linear pressure responses help improve scalability
• Rigid polymeric bead with covalent surface chemistry makes for easier handling and packing, and superior physical and chemical stability, resulting in a robust manufacturing process

Dynamic binding capacity
POROS XS resin has a high binding capacity of more than 100 mg/mL, at 5% breakthrough, over a broad range of process conditions.Higher dynamic binding capacity was achieved by optimizing the base bead pore structure and surface chemistry.

High resolution
Using an optimized 50 µm particle size, POROS XS resin maintains the superior resolving capacity of POROS™ HS resin while overcoming the challenging impurity levels associated with high titer processes. Resolution is consistent across a wide range of operating flow rates and is maintained independent of protein load.

Robust salt tolerance
POROS XS resin has high and consistent protein capacity across a broad range of salt concentrations up to 150 mM NaCl (15 mS/cm conductivity). The ability to load columns under higher conductivity reduces or helps eliminate the dilution of column feed streams or the removal of unit operations, such as tangential flow filtration. The optimized surface ionic capacity of POROS XS resin minimizes competitive binding between the protein of interest and the counterion, resulting in superior salt tolerance.

POROS™ GoPure™ HQ Pre-packed Column, 0.5 x 5 cm, 1 mL Thermo Scientific™

GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS™ ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS™ high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The resin in this GoPure™ HQ 50 µm Column has a quaternized polyethyleneimine functional group for strong anion exchange chromatography.

• Convenient—ready-to-run columns simplify and speed POROS™ resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the GoPure™ family of pre-packed columns simplifies process scale-up and process modeling

GoPure™ 0.5 cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS™ resin-screening platform. The 1 mL bed volume reduces sample requirements, and the recommended > 3 min residence time (0.3 ml/min) on this 5 cm length column equates to 400 cm/hr on a 20 cm bed height. Detailed specifications may be found in the GoPure 0.5 cmD Operating Instructions below.

Other POROS™ anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS™ High Performance Chromatography™ resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, GoPure™ Pre-Packed Columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 ml to 6.3 liters.

POROS™ GoPure™ D Pre-packed Column, 0.5 x 5 cm, 1 mL Thermo Scientific™

GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS™ ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS™ high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The resin in this GoPure™ D 50 µm Column has a dimethylaminopropyl functional group for strong anion exchange chromatography.

• Convenient—ready-to-run columns simplify and speed POROS™ resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the GoPure™ family of pre-packed columns simplifies process scale-up and process modeling

GoPure™ 0.5 cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS™ resin-screening platform. The 1 mL bed volume reduces sample requirements, and the recommended > 3 min residence time (0.3 ml/min) on this 5 cm length column equates to 400 cm/hr on a 20 cm bed height. Detailed specifications may be found in the GoPure 0.5 cmD Operating Instructions below.

Other POROS™ anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS™ High Performance Chromatography™ resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, GoPure™ Pre-Packed Columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 ml to 6.3 liters.

POROS™ GoPure™ PI Pre-packed Column, 0.5 x 5 cm, 1 mL Thermo Scientific™

GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS™ ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS™ high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The resin in this GoPure™ PI 50 µm Column has a polyethyleneimine functional group for strong anion exchange chromatography.

• Convenient—ready-to-run columns simplify and speed POROS™ resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the GoPure™ family of pre-packed columns simplifies process scale-up and process modeling

GoPure™ 0.5 cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS™ resin-screening platform. The 1 mL bed volume reduces sample requirements, and the recommended > 3 min residence time (0.3 ml/min) on this 5 cm length column equates to 400 cm/hr on a 20 cm bed height. Detailed specifications may be found in the GoPure 0.5 cmD Operating Instructions below.

Other POROS™ anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS™ High Performance Chromatography™ resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, GoPure™ Pre-Packed Columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 ml to 6.3 liters.
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