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PrepSEQ™ Nucleic Acid Extraction Kit for Food and Environmental Testing (Applied Biosystems™)

The PrepSEQ® Nucleic Acid Extraction Kit produces high-quality bacterial DNA samples for PCR-based detection from a wide range of food and environmental samples. The quantity and quality of the DNA can greatly impact the success of the sample analysis process and the overall quality of the final result. Food and environmental samples can be challenging to process because they often contain PCR inhibitors. For laboratories looking to achieve better real-time PCR results and improve overall efficiency, the PrepSEQ™ Nucleic Acid Extraction Kit meets these needs.

Automated Sample Preparation for PCR-Based Assays
• Extract high-quality bacterial DNA
• Process a wide range of food and environmental samples
• Fast time-to-results
• Prepare hundreds of samples per shift per instrument

High-Throughput Food and Environmental Sample Preparation
PCR-based bacterial assays have become an important tool in food safety and environmental testing. The Applied Biosystems PrepSEQ™ Nucleic Acid Extraction Kit prepares high-quality bacterial DNA from food and environmental samples for PCR-based testing using magnetic bead-based extraction. In combination with the automated MagMAX™ Express-96 Deep Well Magnetic Particle Processor, this optimized solution enables laboratories to prepare a wide range of sample types at rates of up to hundreds of samples per eight-hour shift.

Streamlined Workflow
An easy-to-use, easy-to-learn protocol simplifies integration of the PrepSEQ™ Nucleic Acid Extraction Kit into the laboratory workflow. The same protocol can be used on a wide range of sample types, and the MagMAX™ Express-96 instrument automates many of the workflow steps to reduce hands-on time.

A Complete Solution
The PrepSEQ™ Nucleic Acid Extraction Kit is part of a fully integrated, start-to-finish solution for food and environmental testing. The kit has been performance-verified for food and environmental testing with MicroSEQ® Microbial Detection Kits, the Applied Biosystems® 7500 Fast Real-Time PCR System, and RapidFinder™ Express Software. For research use only. Not intended for any animal or human therapeutic or diagnostic use.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

MicroSEQ™ Listeria monocytogenes Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit (Applied Biosystems™)

The MicroSEQ® Listeria monocytogenes Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit is recommended for those needing to prepare and analyze hundreds of food or environmental samples per day. The Starter Kit detects Listeria monocytogenes simply, reliably, and rapidly using the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence. The MicroSEQ® Listeria monocytogenes Detection Kit is optimized for use with the PrepSEQ® Nucleic Acid Extraction Kit. For ease of ordering, we have combined a 96 reaction MicroSEQ® Listeria monocytogenes Detection Kit with a 100 reaction PrepSEQ™ Nucleic Acid Extraction Kit and offer it here as a single product.

Features of the MicroSEQ® Listeria monocytogenes Detection Starter Kit:
• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software-guided 3-step process
• Time-to-results of less than 3 hours after sample enrichment (less than 27 hours total)
• Eliminate false negatives with internal positive control
• Detect 1–3 colony-forming units (CFU) per 25 grams of food
• Universal PCR cycling conditions for all MicroSEQ® pathogen detection kits
• Validated by the AOAC Research Institute

Optimized Sample Preparation
The MicroSEQ® Listeria monocytogenes Detection Kit is optimized for use with PrepSEQ® Nucleic Acid Extraction Kit to produce high-quality bacterial DNA samples for PCR-based detection from a wide range of food and environmental samples for detection of 1–3 cfu per 25 grams of sample. In combination with the automated MagMAX™ Express-96 Deep Well Magnetic Particle Processor, this optimized solution enables laboratories to prepare a wide range of sample types at rates of up to hundreds of samples per eight-hour shift.

Lyophilized for Efficiency and Ease of Use
For maximum ease of use, and consistency of results, the reagents used in the MicroSEQ® Listeria monocytogenes Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Universal Fast PCR Cycling Conditions
All MicroSEQ® pathogen detection kits utilize universal PCR cycling conditions which reduce error and allow maximum user flexibility. Fast PCR chemistries are completed in 40 minutes—saving time and clearing presumptive negatives quickly.

Clear Results using a Software-guided Procedure
After sample enrichment and preparation, RapidFinder® Express Software (available separately) guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

AOAC Certified
The full workflow, including the MicroSEQ® Listeria monocytogenes Detection Kit, PrepSEQ® Nucleic Acid Extraction Kit, 7500 Fast instrument and RapidFinder™ Express software, has earned the Performance Tested Methods certification from the AOAC Research Institute.

A Complete Solution
The MicroSEQ® Listeria monocytogenes Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit is part of a complete food testing solution. The kit includes the reagents required to prepare up to 100 sample preparations and run 96 Listeria monocytogenes detection assays. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Listeria monocytogenes detection as fast, easy, and reliable as possible.

For testing of Food and Environmental samples only.

MicroSEQ™ Salmonella spp. Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit (Applied Biosystems™)

The MicroSEQ® Salmonella Species Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit is recommended for those needing to prepare and analyze hundreds of food or environmental samples per day. The Starter Kit detects Salmonella species simply, reliably, and rapidly using the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence. The MicroSEQ® Salmonella Species Detection Kit is optimized for use with the PrepSEQ® Nucleic Acid Extraction Kit. For ease of ordering, we have combined a 96 reaction MicroSEQ® Salmonella Species Detection Kit with a 100 reaction PrepSEQ™ Nucleic Acid Extraction Kit and offer it here as a single product.

Features of the MicroSEQ® Salmonella Species Detection Starter Kit:
• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software-guided 3-step process
• Time-to-results of less than 3 hours after sample enrichment (less than 19 hours total)
• Eliminate false negatives with internal positive control
• Detect 1–3 colony-forming units (CFU) per 25 grams of food
• Universal PCR cycling conditions for all MicroSEQ® pathogen detection kits
• Validated by the AOAC Research Institute

Optimized Sample Preparation
The MicroSEQ® Salmonella Species Detection Kit is optimized for use with PrepSEQ® Nucleic Acid Extraction Kit to produce high-quality bacterial DNA samples for PCR-based detection from a wide range of food and environmental samples for detection of 1–3 cfu per 25 grams of sample. In combination with the automated MagMAX™ Express-96 Deep Well Magnetic Particle Processor, this optimized solution enables laboratories to prepare a wide range of sample types at rates of up to hundreds of samples per eight-hour shift.

Lyophilized for Efficiency and Ease of Use
For maximum ease of use and consistency of results, the reagents used in the MicroSEQ® Salmonella Species Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Universal Fast PCR Cycling Conditions
All MicroSEQ® pathogen detection kits utilize universal PCR cycling conditions which reduce error and allow maximum user flexibility. Fast PCR chemistries are completed in 40 minutes—saving time and clearing presumptive negatives quickly.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder® Express Software (available separately) guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

AOAC Certified
The full workflow including the MicroSEQ® Salmonella Species Detection Kit, PrepSEQ® Nucleic Acid Extraction Kit, 7500 Fast instrument and RapidFinder™ Express software has earned the Performance Tested Methods certification from the AOAC Research Institute.

A Complete Solution
The MicroSEQ® Salmonella Species Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit is part of a complete food testing solution. The kit includes the reagents required to prepare up to 100 sample preparations and run 96 Salmonella species detection assays. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Salmonella species detection as fast, easy, and reliable as possible.

For testing of Food and Environmental samples only.

PrepSEQ™ Rapid Spin Sample Preparation Kit with Proteinase K (Applied Biosystems™)

The PrepSEQ® Rapid Spin Sample Preparation Kit with Proteinase K is recommended for difficult to lyse bacteria like Listeria monocytogenes. The kit was developed for the rapid preparation of DNA from food and environmental samples for use in PCR amplification reactions. Using a simple spin protocol, PrepSEQ™ Rapid Spin efficiently inactivates PCR inhibitors. For laboratories involved in food and environmental testing, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. This has created the demand for a sample preparation method that is fast, cost-effective and capable of extracting high-quality DNA from a broad range of sample types. While such methods have been developed for dedicated, high-throughput laboratories, there is a growing need for methods suitable to low-to-medium throughput applications. The PrepSEQ™ Rapid Spin Kit with Proteinase K meets this need.
• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput work loads (up to 100 samples a day)
• Inactivates PCR inhibitors so you will most likely not have to repeat the template preparation step
• Uses a simple spin protocol

Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from a broad range of sample types including chocolate, soft cheeses, and soil. Even samples with difficult-to-lyse bacteria, such as Listeria monocytogenes, produce excellent results with the addition of a Proteinase K digestion step.

Designed for Laboratories that Prepare up to 100 Samples a Day
Kits exist for dedicated, high-throughput laboratories, but now you have a kit that is suitable for low-to-medium throughput applications. This kit is designed for laboratories that routinely prepare up to 100 samples a day.

Removes PCR Inhibitors
The PrepSEQ™ Rapid Spin Sample Preparation Kit offers a simple, cost-effective way to prepare bacterial DNA from broth cultures. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube where it can be stored or used for PCR.


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For Research Use Only. Not for use in diagnostics procedures.

Lysis Buffer 1 + RNase for Food ID

This convenient packaging of Lysis Buffer 1 + RNase for Food ID is recommended for use with the PrepSEQ Nucleic Acid Extraction Kit (Cat. No. 4480466) to prepare DNA for meat ID and GMO testing from food and feed samples. The protocol using Lysis Buffer 1 + RNase in conjunction with the PrepSEQ Nucleic Acid Extraction Kit can be readily automated on the Thermo Scientific KingFisher mL Food Protection Purification System platform to reduce hands-on time and increase reproducibility of the DNA extraction procedure. The purified DNA can then be used in PCR or other molecular biology techniques, and is well-suited for use with the following food and feed testing kits:

NameCatalog Number
TaqMan GMO Screening Kit4466334
TaqMan Roundup Ready Soya Quantification Kit4466335
TaqMan GMO Maize Quantification Kit4481972
RapidFinder Beef ID KitA24391
RapidFinder Pork ID KitA24392
RapidFinder Equine ID KitA15570
RapidFinder Poultry ID KitA24397
RapidFinder Chicken ID KitA24393
RapidFinder Turkey ID KitA24394
RapidFinder Ruminent ID KitA24396

MicroSEQ™ Salmonella spp. Detection Starter Kit with PrepSEQ™ Rapid Spin Sample Preparation Kit - Extra Clean (Applied Biosystems™)

The MicroSEQ® Salmonella spp. Detection Kit Starter Kit with PrepSEQ™ RapidSpin Sample Preparation Kit – Extra Clean enables the detection of Salmonella spp. simply, reliably, and rapidly in food samples using the Applied Biosystems® 7500 Fast Real-Time PCR System. It is recommended for first time users in laboratories that routinely prepare up to 100 samples a day and prefer a spin column-based workflow. This starter kit combines the PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean with a MicroSEQ Salmonella spp. Detection Kit for ease of ordering. The benefits of this workflow include:

• Enjoy the ease-of-use of a streamlined, lypholized format and simple, software-guided 3-step process
• Get your results quickly with time to results of less than 3 hours after sample enrichment (less than 19 hours total)
• Detect 1-3 colony-forming units (CFUs) per 25 grams of food
• Get optimal performance on a wide range of food sample types
• Eliminate false negatives with an internal positive control
• Validated by the AOAC Research Institute

Simple, Three-step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps:
1. Enrich sample
2. Prepare DNA
3. Run test

Optimized Sample Preparation
The PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean is optimized for use with the MicroSEQ Salmonella spp. Detection Kit. The PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean reduces pre-enrichment time, enhances assay performance, and enables you to enrich and prepare high quality samples from a broad range of food sample types for detection of 1-3 CFUs per 25 grams of sample. The PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean includes extra tubes for a transfer step, which is recommended for foods with a high lipid content, such as infant formula and chicken wing samples. However, simpler matrices can also be prepared with this kit by skipping the transfer step.

Lyophilized for Efficiency and Ease of Use
For maximum ease of use, and consistency of results, the reagents used in the MicroSEQ Salmonella spp. Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Clear Results Using a Software-guided Procedure
After sample enrichment and preparation, RapidFinder™ Express Software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection, and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications, and prompts that enable you to quickly interpret the data and take appropriate action.

AOAC Certified
The full workflow including the MicroSEQ Salmonella spp. Detection Kit, PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean, Applied Biosystems 7500 Fast Real-time PCR System, and RapidFinder Express Software has earned the Performance Tested Methods certification from the AOAC Research Institute.

A Complete Solution
The MicroSEQ Salmonella spp. Detection Kit with PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Salmonella detection as fast, easy and reliable as possible.

MicroSEQ™ Listeria spp Detection Starter Kit, with PrepSEQ™ Rapid Spin Sample Preparation Kit (Applied Biosystems™)

The MicroSEQ® Listeria spp. Detection Kit detects Listeria simply, reliably, and rapidly in food and environmental samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence. This system comes with a PrepSEQ™ Nucleic Acid Extraction Kit.

Key product features:
• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software guided 3-step process
• Get your results quickly with time to results of less than 3 hours after sample enrichment (27-31 hours total)
• Detect 1-3 colony-forming units (CFU) in 25 grams of food or in environmental samples
• Get optimal performance on a wide range of food sample and environmental surface types
• Eliminate false negatives with internal positive control

Lyophilized for Efficiency and Ease of Use
For maximum ease of use, and consistency of results, the reagents used in the MicroSEQ® Listeria spp. Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC helps eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

High Specificity
The specificity of the MicroSEQ® Listeria spp. Detection Kit has been improved through the use of TaqMan® chemistry. Unlike SYBR® Green chemistry, which has only target-specific primers, TaqMan® chemistry has both primers and probes that are specific to the target, an additional way to help prevent false positives.

Simple, Three-Step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps.
1. Enrich sample
2. Prepare DNA
3. Run test

Optimized Sample Preparation
The MicroSEQ® Listeria spp. Detection Kit is optimized for use with PrepSEQ™ Sample Preparation Kits. PrepSEQ™ kits reduce pre-enrichment time, enhance assay performance and enable you to enrich and prepare high quality samples from a broad range of sample types for detection of 1-3 CFU per 25 grams of sample.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder™ Express software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

A Complete Solution
The MicroSEQ® Listeria spp. Detection Kit is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Listeria detection as fast, easy and reliable as possible.

For Research Use Only. Not for use in diagnostics procedures.

MicroSEQ™ E. coli O157:H7 Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit (Applied Biosystems™)

The MicroSEQ® E. coli O157:H7 Detection Kit detects E. coli O157:H7 simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.

Key product features:
• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software guided 3-step process
• Get your results quickly with time-to-results in as little as 8 hours for a 25 g sample
• Genome sequencing using the Applied Biosystems SOLiD™ platform helps ensure high specificity
• Detect 1-3 colony-forming units (CFU) per 25 grams of food
• Get optimal performance on a wide range of food sample and environmental surface types
• Eliminate false negatives with internal positive control
Validated by the AOAC Research Institute and AFNOR according to ISO 16140

Lyophilized for Efficiency and Ease of Use
For maximum ease of use and consistency of results, the reagents used in the MicroSEQ® E. coli O157:H7 Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Enhanced Specificity
To design an assay highly specific for E. coli O157:H7, the genome of E. coli O55:H7 was sequenced using the next-generation Applied Biosystems® SOLiD™ platform and compared to the publicly available sequence of E. coli O157:H7. Sequences specific to E. coli O157:H7 were then used as targets to design the MicroSEQ® E. coli O157:H7 Detection Kit. The MicroSEQ® E. coli O157:H7 Detection Kit owes its additional specificity to the use of TaqMan® chemistry. Unlike SYBR® Green chemistry that uses only target-specific primers, TaqMan® chemistry uses both primers and probes that are specific to the target to help prevent false positives.

Workflow Flexibility
To better meet the needs of food testing laboratories, the MicroSEQ® E. coli O157:H7 Detection Kit offers the flexibility of three different workflows.

Fast Time to Results
• 8-10 hour time-to-results
• 25 g sample
• BHI enrichment
Standard Media
• 18 hour time to results
• 25 g sample
• BPW enrichment
Larger Volumes
• 18 hour time to results
• 375 g sample of ground beef or beef trim
• BPW enrichment

Simple, Three-Step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps.
1. Enrich sample
2. Prepare DNA
3. Run test

AOAC and AFNOR Certified
The full workflow including the MicroSEQ® E. coli O157:H7 Detection Kit, PrepSEQ™ sample preparation kits, 7500 Fast instrument and RapidFinder™ Express software has earned the Performance Tested MethodsSM certification from the AOAC Research Institute, and has been validated by AFNOR according to ISO16140.

Optimized Sample Preparation
The MicroSEQ® E. coli O157:H7 Detection Kit is optimized for use with PrepSEQ™ Sample Preparation Kits. PrepSEQ™ kits reduce pre-enrichment time, enhance assay performance and enable you to enrich and prepare high quality samples from a broad range of sample types for detection of 1-3 CFU per 25 grams of sample.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder™ Express software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

A Complete Solution
The MicroSEQ® E. coli O157:H7 Detection Kit is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make E. coli O15:H7 detection as fast, easy and reliable as possible.

For Research Use Only. Not for use in diagnostics procedures.

PrepSEQ™ Rapid Spin Sample Prep Kit - Extra Clean & Bead Beating (Applied Biosystems™)

The PrepSEQ® Rapid Spin Sample Prep Kit - Extra Clean & Bead Beating was developed for the AFNOR-approved MicroSEQ® Listeria species and Listeria monocytogenes Real-Time PCR detection workflows. A spin, transfer, and bead beating protocol efficiently lyses Listeria from a 750 µL aliquot of more challenging enriched food samples (e.g., samples containing high fat or otherwise prone to PCR inhibition). The PrepSEQ® Rapid Spin Sample Prep Kit - Extra Clean & Bead Beating meets the needs of lower throughput food laboratories.

NOTE: Standard samples that do not contain high fat or demonstrate PCR inhibition should be processed using the PrepSEQ® Rapid Spin Sample Prep Kit - Bead Beating.

• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput (up to 100 samples a day)
• Inactivates PCR inhibitors and produces high quality DNA for downstream detection
• Uses a simple spin protocol

Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from the most challenging sample types.

Designed for Laboratories that Prepare up to 100 Samples a Day
A spin column-based preparation enables small batch sizes to be managed while requiring only simple benchtop devices to process, thereby limiting the capital expenditure associated with much higher throughput automation devices.

Removes PCR Inhibitors and Produces High Quality DNA
The PrepSEQ® Rapid Spin Sample Prep Kit - Extra Clean & Bead Beating offers a simple, cost-effective way to prepare Listeria DNA from more difficult starting matrices such as cheese, milk, and high fat meats. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube. The pellet is resuspended, then transferred (to avoid carryover, which inhibits PCR) to a new tube containing zirconium beads for mechanical lysis.

MicroSEQ™ Listeria spp. Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit (Applied Biosystems™)

The MicroSEQ® Listeria spp. Detection Kit detects Listeria simply, reliably, and rapidly in food and environmental samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence. This system comes with a PrepSEQ™ Nucleic Acid Extraction Kit.

Key product features:
• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software guided 3-step process
• Get your results quickly with time to results of less than 3 hours after sample enrichment (27-31 hours total)
• Detect 1-3 colony-forming units (CFU) in 25 grams of food or in environmental samples
• Get optimal performance on a wide range of food sample and environmental surface types
• Eliminate false negatives with internal positive control

Lyophilized for Efficiency and Ease of Use
For maximum ease of use, and consistency of results, the reagents used in the MicroSEQ® Listeria spp. Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC helps eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

High Specificity
The specificity of the MicroSEQ® Listeria spp. Detection Kit has been improved through the use of TaqMan® chemistry. Unlike SYBR® Green chemistry, which has only target-specific primers, TaqMan® chemistry has both primers and probes that are specific to the target, an additional way to help prevent false positives.

Simple, Three-Step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps.
1. Enrich sample
2. Prepare DNA
3. Run test

Optimized Sample Preparation
The MicroSEQ® Listeria spp. Detection Kit is optimized for use with PrepSEQ™ Sample Preparation Kits. PrepSEQ™ kits reduce pre-enrichment time, enhance assay performance and enable you to enrich and prepare high quality samples from a broad range of sample types for detection of 1-3 CFU per 25 grams of sample.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder™ Express software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

A Complete Solution
The MicroSEQ® Listeria spp. Detection Kit is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Listeria detection as fast, easy and reliable as possible.

For Research Use Only. Not for use in diagnostics procedures.

PrepSEQ™ Rapid Spin Sample Prep Kit - Bead Beating (Applied Biosystems™)

The PrepSEQ® Rapid Spin Sample Prep Kit - Bead Beating was developed for the AFNOR-approved MicroSEQ® Listeria species and Listeria monocytogenes Real-Time PCR detection workflows. A combined spin and bead beating protocol efficiently lyses Listeria from a 750 µL aliquot of enriched food samples (those with little-to-no fat and lean meats) and simple environmental samples (swabs and sponges). The PrepSEQ® Rapid Spin Sample Prep Kit - Bead Beating is designed to meet the needs of lower throughput food laboratories.

NOTE: Difficult samples (e.g., samples containing high fat or otherwise prone to PCR inhibition) should be processed using the PrepSEQ® Rapid Spin Sample Prep Kit - Extra Clean & Bead Beating.

• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput (up to 100 samples a day)
• Inactivates PCR inhibitors and produces high quality DNA for downstream detection
• Uses a simple spin protocol

Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from a broad range of sample types.

Designed for Laboratories that Prepare up to 100 Samples a Day
Spin column-based preparation enables small batch sizes to be managed while requiring only simple benchtop devices to process, thereby limiting the capital expenditure associated with much higher throughput automation devices.

Removes PCR Inhibitors and Produces High Quality DNA
The PrepSEQ® Rapid Spin Sample Prep Kit - Bead Beating offers a simple, cost-effective way to prepare Listeria DNA from enriched cultures. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube, where they are then lysed via a zirconium bead beating step.

MicroSEQ™ Listeria monocytogenes Detection Starter Kit, with PrepSEQ™ Rapid Spin Sample Preparation Kit (Applied Biosystems™)

The MicroSEQ® Listeria monocytogenes Detection Kit Starter Kit is recommended for first time users in laboratories that routinely prepare up to 100 samples a day. It detects Listeria monocytogenes simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.
• Enjoy ease-of-use from a streamlined, lypholized format and a simple, software guided 3-step process
• Get your results quickly with time to results of less than 3 hours after sample enrichment (less than 27 hours total)
• Detect 1-3 colony-forming units (CFU) per 25 grams of food
• PrepSEQ™ Rapid Spin Sample Preparation Kit included
• Get optimal performance on a wide range of food sample types
• Eliminate false negatives with internal positive control
• Validated by the AOAC Research Institute

Lyophilized for Efficiency and Ease of Use
For maximum ease of use, and consistency of results, the reagents used in the MicroSEQ® Listeria monocytogenes Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC) and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Simple, Three-Step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps.
1. Enrich sample
2. Prepare DNA
3. Run test

AOAC Certified
The full workflow including the MicroSEQ® Listeria monocytogenes Detection Kit, PrepSEQ™ sample preparation kits, 7500 Fast instrument and RapidFinder Express software has earned the Performance Tested Methods certification from the AOAC Research Institute.

Optimized Sample Preparation
The MicroSEQ® Listeria monocytogenes Detection Kit is optimized for use with PrepSEQ™ Sample Preparation Kits. PrepSEQ™ kits reduce pre-enrichment time, enhance assay performance and enable you to enrich and prepare high quality samples from a broad range of food sample types for detection of 1-3 cfu per 25 grams of sample.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder® Express software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the Software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

A Complete Solution
The MicroSEQ® Listeria monocytogenes Detection Kit is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Listeria detection as fast, easy and reliable as possible.

For Research Use Only. Not for use in diagnostics procedures.

MicroSEQ™ E. coli O157:H7 Detection Starter Kit, with PrepSEQ™ Rapid Spin Sample Preparation Kit (Applied Biosystems™)

The MicroSEQ® E. coli O157:H7 Detection Kit detects E. coli O157:H7 simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.

Key product features:
• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software guided 3-step process
• Get your results quickly with time-to-results in as little as 8 hours for a 25g sample
• Genome sequencing using the Applied Biosystems SOLiD™ platform helps ensure high specificity
• Detect 1-3 colony-forming units (CFU) per 25 grams of food
• Get optimal performance on a wide range of food sample and environmental surface types
• Eliminate false negatives with internal positive control

Lyophilized for Efficiency and Ease of Use
For maximum ease of use and consistency of results, the reagents used in the MicroSEQ® E. coli O157:H7 Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Enhanced Specificity
To design an assay highly specific for E. coli O157:H7, the genome of E. coli O55:H7 was sequenced using the next-generation Applied Biosystems® SOLiD™ platform and compared to the publicly available sequence of E. coli O157:H7. Sequences specific to E. coli O157:H7 were then used as targets to design the MicroSEQ® E. coli O157:H7 Detection Kit. The MicroSEQ® E. coli O157:H7 Detection Kit owes its additional specificity to the use of TaqMan® chemistry. Unlike SYBR® Green chemistry that uses only target-specific primers, TaqMan® chemistry uses both primers and probes that are specific to the target to help prevent false positives.

Workflow Flexibility
To better meet the needs of food testing laboratories, the MicroSEQ® E. coli O157:H7 Detection Kit offers the flexibility of three different workflows.

Fast Time to Results
• 8-10 hour time-to-results
• 25 g sample
• BHI enrichment
Standard Media
• 18 hour time to results
• 25 g sample
• BPW enrichment
Larger Volumes
• 18 hour time to results
• 375 g sample of ground beef or beef trim
• BPW enrichment

Simple, Three-Step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps.
1. Enrich sample
2. Prepare DNA
3. Run test

AOAC Certified
The full workflow including the MicroSEQ® E. coli O157:H7 Detection Kit, PrepSEQ™ sample preparation kits, 7500 Fast instrument and RapidFinder™ Express software has earned the Performance Tested MethodsSM certification from the AOAC Research Institute.

Optimized Sample Preparation
The MicroSEQ® E. coli O157:H7 Detection Kit is optimized for use with PrepSEQ™ Sample Preparation Kits. PrepSEQ™ kits reduce pre-enrichment time, enhance assay performance and enable you to enrich and prepare high quality samples from a broad range of sample types for detection of 1-3 CFU per 25 grams of sample.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder™ Express software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

A Complete Solution
The MicroSEQ® E. coli O157:H7 Detection Kit is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make E. coli O15:H7 detection as fast, easy and reliable as possible.

For Research Use Only. Not for use in diagnostics procedures.

PrepSEQ™ Rapid Spin Sample Preparation Kit - Extra Clean (Applied Biosystems™)

The PrepSEQ™ Rapid Spin Sample Preparation Kit – Extra Clean is recommended for foods with a high lipid content, such as infant formula and chicken wing samples. The kit was developed for the rapid preparation of DNA from food and environmental samples for use in PCR amplification reactions. Using a simple spin protocol, PrepSEQ™ Rapid Spin efficiently inactivates PCR inhibitors. For laboratories involved in food and environmental testing, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. This has created the demand for a sample preparation method that is fast, cost-effective and capable of extracting high-quality DNA from a broad range of sample types. While such methods have been developed for dedicated, high-throughput laboratories, there is a growing need for methods suitable to low-to-medium throughput applications. The PrepSEQ™ Rapid Spin Kit – Extra Clean meets this need.

• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput work loads (up to 100 samples a day)
• Inactivates PCR inhibitors so you will most likely not have to repeat the template preparation step
• Uses a simple spin protocol
Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from a broad range of sample types including chocolate, soft cheeses, and soil.

Designed for Laboratories that Prepare up to 100 Samples a Day
Kits exist for dedicated, high-throughput laboratories, but now you have a kit that is suitable for low-to-medium throughput applications. This kit is designed for laboratories that routinely prepare up to 100 samples a day.

Removes PCR Inhibitors
The PrepSEQ™ Rapid Spin Sample Preparation Kit offers a simple, cost-effective way to prepare bacterial DNA from broth cultures. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube where it can be stored or used for PCR.

For Research Use Only. Not for use in diagnostics procedures.