Products

Pierce™ Primary Neuron Isolation Kit Thermo Scientific™

The Thermo Scientific Pierce Primary Neuron Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary neurons from embryonic cortical and hippocampal tissue from mouse and rat.

Features of the Primary Neuron Isolation Kit:

Optimized—procedure and reagents optimized for viability, yield, purity, and ease-of-use
Yield—provides a 2-fold increase in yield compared to do-it-yourself methods
Viability—greater than 95% of isolated cells remain viable in culture from Day 1 to Week 4
Functional—cultured neurons contain processes indicative of complete differentiation and functionality

The Primary Neuron Isolation Kit has been designed to obtain high viability and purity of primary embryonic neurons using validated reagents and an optimized procedure for enzymatic digestion and culturing. The kit contains tissue-specific dissociation enzymes, media formulated specifically for neuronal cell culture, and cell culture supplements that promote the isolation and growth of functional neurons. The validated protocol and reagents produce highly pure primary neurons for either short- or long-term culture, and the kit is designed for use by both experienced and non-experienced users.

Includes:
• Complete kit includes Neuronal Isolation Enzyme, Hanks' Balanced Salt Solution (HBSS), Neuronal Culture Medium, Neuronal Culture Media Supplement, Glutamine Supplement, and Neuronal Growth Supplement

Requires:
• Fresh embryonic mouse or rat tissue sample, heat-inactivated fetal bovine serum (FBS), poly-D-lysine treated culture dishes

Applications:
• Neuronal cell differentiation
• Immunohistochemistry (IHC)
• Functional and biochemical assays
• Biologically relevant system for preclinical drug discovery, neurotoxicity testing, and predictive disease modeling

The Pierce Primary Neuron Isolation Kit has been optimized to provide excellent cell yield and to sustain cells at greater than 95% viability. The neurons are appropriately polarized, develop extensive axonal and dendritic arbors, express neuronal and synaptic markers, and form numerous, functional synaptic connections with one another. They can be used as a model system for molecular and cellular biology studies of neuronal development and function, especially for visualizing the subcellular localization of endogenous or expressed proteins, imaging protein, neuronal polarity, and dendritic growth and synapse formation [1].

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An efficient method for the isolation of highly functional primary neurons

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Neuronal Culture Medium for Pierce™ Primary Cell Isolation Kits
Neuron Isolation Enzyme (with papain) for Pierce™ Primary Cell Isolation Kits
Neuronal Culture Media Supplement (50X) for Pierce™ Primary Cell Isolation Kits
N-PER™ Neuronal Protein Extraction Reagent

Neuron Isolation Enzyme (with papain) for Pierce™ Primary Cell Isolation Kits Thermo Scientific™

Neuronal Isolation Enzyme (with papain) is a tissue-specific dissociation reagent for the isolation of primary cortical and hippocampal neurons from mouse/rat embryonic brain tissue and is a component of the Thermo Scientific™ Pierce™ Primary Neuron Isolation Kit (Product No. 88280).

Papain has been demonstrated to be more effective and less harsh than other proteases for tissue dissociation. When used with Cardiomyocyte Isolation Enzyme 2 (with thermolysin), it improves digestion efficiency and cell yield of primary cardiomyocytes.

Papain Information:

Source:Papaya latex
Molecular mass: 23kDa
Optimal pH: 6.0-7.0

More Product Data
An efficient method for the isolation of highly functional primary neurons

Related Products
Pierce™ Primary Neuron Isolation Kit
Neuronal Culture Medium for Pierce™ Primary Cell Isolation Kits
Neuronal Culture Media Supplement (50X) for Pierce™ Primary Cell Isolation Kits
N-PER™ Neuronal Protein Extraction Reagent

DMEM for Pierce™ Primary Cell Isolation Kits Thermo Scientific™

The Thermo Scientific Pierce DMEM for Primary Cell Isolation is classical Dulbecco's Modified Eagle's Medium - High Glucose, which has been shown to be ideal for the isolation of primary cells, including Cardiomyocytes. The Thermo Scientific Pierce Primary Cardiomyocyte Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary cardiomyocytes from neonatal heart from mouse and rat.

Features of Thermo Scientific Pierce DMEM for Primary Cell Isolation:

Optimized – procedure and reagents optimized for viability, yield, purity, and ease-of-use
Time-saving – isolation protocol requires less than 2 hours compared to up to 21 hours by other methods
Yield – provides a 1.5- to 4-fold increase in yield compared to do-it-yourself methods and other commercial kits
Viability – provides higher viability than do-it-yourself methods and other commercial kits
Functional – cultured cardiomyocytes express the appropriate biochemical markers with validated beating activity

Includes:
• Complete kit includes Cardiomyocyte Isolation Enzyme 1, Cardiomyocyte Isolation Enzyme 2, Hanks' Balanced Salt Solution (HBSS), DMEM for Primary Cell Isolation, and Cardiomyocyte Growth Supplement

Applications:
• Cardiomyocyte cell differentiation
• Immunohistochemistry (IHC)
• Functional and biochemical assays
• Biologically relevant system for preclinical drug discovery and predictive disease modeling

The Primary Cardiomyocyte Isolation Kit has been designed to obtain high viability and purity of primary cardiomyocytes using validated reagents and an optimized procedure for enzymatic digestion and culturing. The kit contains two tissue-specific dissociation enzymes, media formulated for primary cell culture, and a cardiomyocyte cell culture growth supplement that promotes the isolation, enrichment and growth of functional cardiomyocytes. The validated protocol ensures optimal isolation and significantly reduces the processing time of isolation when compared to other methods. Addition of the cardiomyocyte growth supplement to culture cells further enriches for cardiomycytes, and cultures have been maintained for up to 7 days using the kit components.

Current methods for dissociation of cardiac cells from heart tissue require repeated (5 to 8 incubations) or lengthy (>16 hours) enzyme digestion, resulting in reduced yield and viability of isolated cells. The Pierce Primary Cardiomyocyte Isolation Kit provides fully optimized reagents and a protocol that prevents overdigestion for the isolation and culture of cardiomyocytes. The complete process from handling primary tissues to seeding cells in culture vessels can be completed within 2 hours.

Because it uses optimized tissue-specific dissociation enzymes, this kit isolates cardiomyocytes with greater yield and cell viability than cells isolated by existing methods. The isolated primary cardiomyocytes express cardiomyocyte protein markers and exhibit contractile function (beating activity, see video), thereby providing a model system for studies of contraction, ischaemia, hypoxia and the toxicity of various compounds.

   

More Product Data
One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts

Related Products
Pierce™ Primary Cardiomyocyte Isolation Kit
Cardiomyocyte Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits
Cardiomyocyte Isolation Enzyme 2 (with thermolysin) (20X) for Pierce™ Primary Cell Isolation Kits
Hanks' Balanced Salt Solution (HBSS) without Ca2+, Mg2+ for Pierce™ Primary Cell Isolation Kits

Hanks' Balanced Salt Solution (HBSS) without Ca2+, Mg2+ for Pierce™ Primary Cell Isolation Kits Thermo Scientific™

The Thermo Scientific Pierce Hanks' Balanced Salt Solution (HBSS) without Ca2+, Mg2+ is ideal for gently washing primary cells after digestion of the connective tissue. The Thermo Scientific Pierce Primary Cardiomyocyte Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary cardiomyocytes from neonatal heart from mouse and rat.

Features of the Thermo Scientific Pierce Hanks' Balanced Salt Solution (HBSS):

Optimized – procedure and reagents optimized for viability, yield, purity, and ease-of-use
Time-saving – isolation protocol requires less than 2 hours compared to up to 21 hours by other methods
Yield – provides a 1.5- to 4-fold increase in yield compared to do-it-yourself methods and other commercial kits
Viability – provides higher viability than do-it-yourself methods and other commercial kits
Functional – cultured cardiomyocytes express the appropriate biochemical markers with validated beating activity

Includes:
• Complete kit includes Cardiomyocyte Isolation Enzyme 1, Cardiomyocyte Isolation Enzyme 2, Hanks' Balanced Salt Solution (HBSS), DMEM for Primary Cell Isolation, and Cardiomyocyte Growth Supplement

Applications:
• Cardiomyocyte cell differentiation
• Immunohistochemistry (IHC)
• Functional and biochemical assays
• Biologically relevant system for preclinical drug discovery and predictive disease modeling

The Primary Cardiomyocyte Isolation Kit has been designed to obtain high viability and purity of primary cardiomyocytes using validated reagents and an optimized procedure for enzymatic digestion and culturing. The kit contains two tissue-specific dissociation enzymes, media formulated for primary cell culture, and a cardiomyocyte cell culture growth supplement that promotes the isolation, enrichment and growth of functional cardiomyocytes. The validated protocol ensures optimal isolation and significantly reduces the processing time of isolation when compared to other methods. Addition of the cardiomyocyte growth supplement to culture cells further enriches for cardiomycytes, and cultures have been maintained for up to 7 days using the kit components.

Current methods for dissociation of cardiac cells from heart tissue require repeated (5 to 8 incubations) or lengthy (>16 hours) enzyme digestion, resulting in reduced yield and viability of isolated cells. The Pierce Primary Cardiomyocyte Isolation Kit provides fully optimized reagents and a protocol that prevents overdigestion for the isolation and culture of cardiomyocytes. The complete process from handling primary tissues to seeding cells in culture vessels can be completed within 2 hours.

Because it uses optimized tissue-specific dissociation enzymes, this kit isolates cardiomyocytes with greater yield and cell viability than cells isolated by existing methods. The isolated primary cardiomyocytes express cardiomyocyte protein markers and exhibit contractile function (beating activity, see video), thereby providing a model system for studies of contraction, ischaemia, hypoxia and the toxicity of various compounds.

   

More Product Data
One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts

Related Products
Pierce™ Primary Cardiomyocyte Isolation Kit
Cardiomyocyte Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits
Cardiomyocyte Isolation Enzyme 2 (with thermolysin) (20X) for Pierce™ Primary Cell Isolation Kits
DMEM for Pierce™ Primary Cell Isolation Kits

Pierce™ Primary Cardiomyocyte Isolation Kit Thermo Scientific™

The Thermo Scientific Pierce Primary Cardiomyocyte Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary cardiomyocytes from neonatal heart from mouse and rat.

Features of the Primary Cardiomyocyte Isolation Kit:

Optimized – procedure and reagents optimized for viability, yield, purity, and ease-of-use
Time-saving – isolation protocol requires less than 2 hours compared to up to 21 hours by other methods
Yield – provides a 1.5- to 4-fold increase in yield compared to do-it-yourself methods and other commercial kits
Viability – provides higher viability than do-it-yourself methods and other commercial kits
Functional – cultured cardiomyocytes express the appropriate biochemical markers with validated beating activity

The Primary Cardiomyocyte Isolation Kit has been designed to obtain high viability and purity of primary cardiomyocytes using validated reagents and an optimized procedure for enzymatic digestion and culturing. The kit contains two tissue-specific dissociation enzymes, media formulated for primary cell culture, and a cardiomyocyte cell culture growth supplement that promotes the isolation, enrichment and growth of functional cardiomyocytes. The validated protocol ensures optimal isolation and significantly reduces the processing time of isolation when compared to other methods. Addition of the cardiomyocyte growth supplement to culture cells further enriches for cardiomycytes, and cultures have been maintained for up to 7 days using the kit components.

Includes:
• Complete kit includes Cardiomyocyte Isolation Enzyme 1, Cardiomyocyte Isolation Enzyme 2, Hanks' Balanced Salt Solution (HBSS), DMEM for Primary Cell Isolation, and Cardiomyocyte Growth Supplement

Applications:
• Cardiomyocyte cell differentiation
• Immunohistochemistry (IHC)
• Functional and biochemical assays
• Biologically relevant system for preclinical drug discovery and predictive disease modeling

Current methods for dissociation of cardiac cells from heart tissue require repeated (5 to 8 incubations) or lengthy (>16 hours) enzyme digestion, resulting in reduced yield and viability of isolated cells. The Pierce Primary Cardiomyocyte Isolation Kit provides fully optimized reagents and a protocol that prevents overdigestion for the isolation and culture of cardiomyocytes. The complete process from handling primary tissues to seeding cells in culture vessels can be completed within 2 hours.

Because it uses optimized tissue-specific dissociation enzymes, this kit isolates cardiomyocytes with greater yield and cell viability than cells isolated by existing methods. The isolated primary cardiomyocytes express cardiomyocyte protein markers and exhibit contractile function (beating activity, see video), thereby providing a model system for studies of contraction, ischaemia, hypoxia and the toxicity of various compounds.

   

More Product Data
One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts

Related Products
Cardiomyocyte Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits
Cardiomyocyte Isolation Enzyme 2 (with thermolysin) (20X) for Pierce™ Primary Cell Isolation Kits
DMEM for Pierce™ Primary Cell Isolation Kits
Hanks' Balanced Salt Solution (HBSS) without Ca2+, Mg2+ for Pierce™ Primary Cell Isolation Kits

Neuronal Culture Media Supplement (50X) for Pierce™ Primary Cell Isolation Kits Thermo Scientific™

Neuronal Culture Media Supplement is a proprietary reagent that has been formulated for optimal growth of primary mouse and rat neurons. The supplement has been formulated for use with Neuronal Culture Medium (Product No. 88283) and is a component of the Thermo Scientific™ Pierce™ Primary Neuron Isolation Kit (Product No. 88280).

More Product Data
An efficient method for the isolation of highly functional primary neurons

Related Products
Pierce™ Primary Neuron Isolation Kit
Neuronal Culture Medium for Pierce™ Primary Cell Isolation Kits
Neuron Isolation Enzyme (with papain) for Pierce™ Primary Cell Isolation Kits
N-PER™ Neuronal Protein Extraction Reagent

Neuronal Culture Medium for Pierce™ Primary Cell Isolation Kits Thermo Scientific™

The Thermo Scientific Pierce Primary Neuron Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary neurons form embryonic cortical and hippocampal tissue from mouse and rat.

Features of The Thermo Scientific Pierce Primary Neuron Isolation Kit:

Optimized—procedure and reagents optimized for viability, yield, purity, and ease-of-use
Yield—provides a 2-fold increase in yield compared to do-it-yourself methods
Viability—greater than 95% of isolated cells remain viable in culture from Day 1 to Week 4
Functional—cultured neurons contain processes indicative of complete differentiation and functionality

Includes:
• Complete kit includes Neuronal Isolation Enzyme, Hanks' Balanced Salt Solution (HBSS), Neuronal Culture Medium, Neuronal Culture Media Supplement, Glutamine Supplement, and Neuronal Growth Supplement

Requires:
• Fresh embryonic mouse or rat tissue sample, heat-inactivated fetal bovine serum (FBS), poly-D-lysine treated culture dishes

Applications:
• Neuronal cell differentiation
• Immunohistochemistry (IHC)
• Functional and biochemical assays
• Biologically relevant system for preclinical drug discovery, neurotoxicity testing, and predictive disease modeling

The Primary Neuron Isolation Kit has been designed to obtain high viability and purity of primary embryonic neurons using validated reagents and an optimized procedure for enzymatic digestion and culturing. The kit contains tissue-specific dissociation enzymes, media formulated specifically for neuronal cell culture, and cell culture supplements that promote the isolation and growth of functional neurons. The validated protocol and reagents produce highly pure primary neurons for either short- or long-term culture, and the kit is designed for use by both experienced and non-experienced users.

The Pierce Primary Neuron Isolation Kit has been optimized to provide excellent cell yield and to sustain cells at greater than 95% viability. The neurons are appropriately polarized, develop extensive axonal and dendritic arbors, express neuronal and synaptic markers, and form numerous, functional synaptic connections with one another. They can be used as a model system for molecular and cellular biology studies of neuronal development and function, especially for visualizing the subcellular localization of endogenous or expressed proteins, imaging protein, neuronal polarity, and dendritic growth and synapse formation [1].

More Product Data
An efficient method for the isolation of highly functional primary neurons

Related Products
Pierce™ Primary Neuron Isolation Kit
Neuron Isolation Enzyme (with papain) for Pierce™ Primary Cell Isolation Kits
Neuronal Culture Media Supplement (50X) for Pierce™ Primary Cell Isolation Kits
N-PER™ Neuronal Protein Extraction Reagent

Mouse Embryonic Fibroblast Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits Thermo Scientific™

Thermo Scientific Pierce Mouse Embryonic Fibroblast Isolation Enzyme (with papain) is a tissue-specific dissociation reagent for isolation of MEFs from mouse embryonic tissue and is a component of the Thermo Scientific™ Pierce™ Mouse Embryonic Fibroblast Isolation Kit.

Papain has been demonstrated to be more effective and less harsh than other proteases for tissue dissociation. At its optimal concentration, this reagent ensures a high yield of viable and functional fibroblasts.

More Product Data
An improved method for highly efficient isolation of primary mouse embryonic fibroblasts

Related Products
Pierce™ Mouse Embryonic Fibroblast Isolation Kit

Cardiomyocyte Isolation Enzyme 2 (with thermolysin) (20X) for Pierce™ Primary Cell Isolation Kits Thermo Scientific™

Cardiomyocyte Isolation Enzyme 2 (with thermolysin) is a tissue-specific dissociation reagent for the isolation of primary cardiomyocytes from neonatal heart tissue from mouse/rat and is a component of the Thermo Scientific™ Pierce™ Primary Cardiomyocyte Isolation Kit (Product No. 88281).

Thermolysin is a thermostable extracellular metalloendopeptidase. When used with Cardiomyocyte Isolation Enzyme 1 (with papain), it improves digestion efficiency and cell yield of primary cardiomyocytes.

Thermolysin Information:
Source: Bacillus thermoproteolyticus rokko
Molecular mass: 34.6kDa

More Product Data
One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts

Related Products
Pierce™ Primary Cardiomyocyte Isolation Kit
Cardiomyocyte Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits
DMEM for Pierce™ Primary Cell Isolation Kits
Hanks' Balanced Salt Solution (HBSS) without Ca2+, Mg2+ for Pierce™ Primary Cell Isolation Kits

Pierce™ Mouse Embryonic Fibroblast Isolation Kit Thermo Scientific™

The Thermo Scientific Pierce Mouse Embryonic Fibroblast Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of fibroblasts from mouse embryos.

Features of the Mouse Embryonic Fibroblast Isolation Kit:

Optimized – procedure and reagents optimized for viability, yield, purity, and ease-of-use
Yield – provides a 2-fold increase in yield compared to do-it-yourself methods and other kits
Functional – cultured fibroblasts express the appropriate biochemical markers

Includes:
• Complete kit includes Fibroblast Isolation Enzyme, Hanks' Balanced Salt Solution (HBSS), and DMEM for Primary Cell Isolation

The Mouse Embryonic Fibroblast Isolation Kit has been designed to obtain high viability and purity of embryonic fibroblasts using validated reagents and an optimized procedure. The kit contains a tissue-specific dissociation enzyme and media formulated for primary cell culture that promotes the isolation growth of functional fibroblasts. In addition, MEF isolated and cultured with the Pierce MEF Isolation Kit can be cryopreserved for future use with very minimal loss of viability.

Applications:
• Generation of immortalized cell lines from transgenic mice
• Feeder layer for stem cells
• IHC and IF studies

Because it uses an optimized tissue-specific dissociation enzyme, this kit isolates fibroblasts with greater yield and cell viability than cells isolated by existing methods. Isolated MEF express fibroblast-specific protein markers, confirming their viability and unperturbed metabolic function. After isolation, the mouse fibroblasts may be immortalized by continuous passage for the establishment of unique cell lines. They may also be used as a cell culture model for a diverse range of studies such as gene regulation and stem cell research [1,2,3,4].

More Product Data
An improved method for highly efficient isolation of primary mouse embryonic fibroblasts

Related Products
Mouse Embryonic Fibroblast Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits

CTS™ OpTmizer™ T Cell Expansion SFM, bag format Gibco™

Gibco® OpTmizer™ CTS™ T-Cell Expansion SFM has been developed for the growth and expansion of human T lymphocytes. OpTmizer™ CTS™ T-Cell Expansion medium is a complete serum-free and xeno-free 1X medium consisting of OpTmizer™ T-Cell Expansion Basal Medium (1 L bag) and OpTmizer™ T-Cell Expansion Supplement (26 mL), which are mixed together prior to use.

• Maintains similar phenotype and function as T-cells cultured in conventional serum supplemented medium
• Consistent performance in supporting T-cell expansion
• Supports high density cultures in static and WAVE Bioreactor® cultures
• Be ready for clinical research with this cGmP manufactured formula and support documentation

Versatile T Cell Culture Media
OpTmizer™ CTS™ T-Cell Expansion SFM is designed to give results equivalent to conventional serum-supplemented media. However, if desired, 2% heat-inactivated human serum (Cat. No. 34005-100) may be added to the medium to enhance viability and expansion. The use of serum, and the amount required, should be determined empirically depending on the specific T-cell culture application.

Ready for T Cell Expansion
OpTmizer™ CTS™ T-Cell Expansion SFM is designed for the expansion of human T-cells permitting CD3+ densities of >3x106 cells/mL in static conditions and >2x107 cells/mL in WAVE bags. Results may vary depending on the T-cell subset (e.g., Ag-specific cells). Conditions for specific T-cell culture applications should be determined empirically.

Stability of Complete Media
Complete OpTmizer™ CTS™ T-Cell Expansion SFM (basal, supplement, and L-glutamine) is stable for 4 weeks when stored in the dark at 2–8°C.

Simplify Your Transition into Clinical Research
The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco® CTS™ products deliver:
• Reduced burden in qualifying reagents
• Appropriate certificates of analyses and origin included
• Convenient access to our Drug Master File (DMF)

CTS™ OpTmizer™ T Cell Expansion SFM, bottle format Gibco™

GIBCO OpTmizer™ CTS™ T-Cell Expansion SFM has been developed for the growth and expansion of human T lymphocytes. OpTmizer™ CTS™ T-Cell Expansion medium is a complete serum-free, xeno-free 1X medium consisting of OpTmizer™ T-Cell Expansion Basal Medium (1 L bottle) and OpTmizer™ T-Cell Expansion Supplement (26 mL), which are mixed together prior to use.

• Maintains similar phenotype and function (e.g., cytokine secretion profile) of polyclonally activated and cultured T cells as observed for conventional serum supplemented medium
• Consistent performance in supporting T cell expansion
• Supports high density T cell culture (e.g., >3x106 CD3+ T cells/mL) in static culture

With OpTmizer™ T-Cell Expansion SFM, we offer both basic and clinical research communities a new tool to enhance their success in growing human T-cells. It is specially formulated to allow for superior cell growth & viability, less variability and more consistent results, and easier transition from research to clinical applications.

Workflow benefits:
• Minimizing the need to handle purchasing, storage, qualification of serum and thus reducing variability and costs
• More rapid T cell expansion, thus reducing cycle time and improving productivity
• Complete serum-free, xeno-free medium, allowing for a seamless transition from research to clinical applications and minimizing regulatory workload
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