Products

Propidium Iodide - 1.0 mg/mL Solution in Water Invitrogen™

Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.

PI binds to DNA by intercalating between the bases with little or no sequence preference. In aqueous solution, the dye has excitation/emission maxima of 493/636 nm. Once the dye is bound, its fluorescence is enhanced 20- to 30-fold, the fluorescence excitation maximum is shifted ~30–40 nm to the red and the fluorescence emission maximum is shifted ~15 nm to the blue, resulting in an excitation maximum at 535 nm and fluorescence emission maximum at 617 nm.

PI is widely used in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry.

Learn more about propidium iodide, and propidium iodide containing products

Propidium Iodide Invitrogen™

Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.

PI binds to DNA by intercalating between the bases with little or no sequence preference. In aqueous solution, the dye has excitation/emission maxima of 493/636 nm. Once the dye is bound, its fluorescence is enhanced 20- to 30-fold, the fluorescence excitation maximum is shifted ~30–40 nm to the red and the fluorescence emission maximum is shifted ~15 nm to the blue, resulting in an excitation maximum at 535 nm and fluorescence emission maximum at 617 nm.

PI is widely used in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry.

Learn more about propidium iodide, and propidium iodide containing products

Propidium Iodide - FluoroPure™ Grade Invitrogen™

Because it is manufactured at our ISO 9001—certified facilities in Eugene, Oregon, we can guarantee that FluoroPure grade propidium iodide is greater than or equal to 98% pure by HPLC.

Also available as a room-temperature-stable, ready-to-use solution: Propidium Iodide Ready Probes Reagent.
See other Ready Probes ready-to-use imaging reagents and accessories ›

eBioscience™ Propidium Iodide Staining Solution Invitrogen™

Propidium Iodide (PI) is a ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. PI binds to double stranded DNA by intercalating between basepairs, but is excluded from cells with intact plasma membranes. PI can be used in FL3 for inviability exclusion, but should be analyzed in FL2 when used as a counterstain for FITC Annexin V.

Reported Application
Flow Cytometric Analysis

Vybrant™ Apoptosis Assay Kit #4, YO-PRO™-1/Propidium Iodide Invitrogen™

The Vybrant Apoptosis Assay Kit #4 detects apoptosis on the basis of changes that occur in the permeability of cell membranes. This kit contains ready-to-use solutions of both YO-PRO-1 and propidium iodide nucleic acid stains. YO-PRO-1 stain selectively passes through the plasma membranes of apoptotic cells and labels them with moderate green fluorescence. Necrotic cells are stained red-fluorescent with propidium iodide.

View a selection guide for all apoptosis assays for flow cytometry.

eBioscience™ Propidium Iodide Invitrogen™

Propidium Iodide (PI) is a standard reagent used for assessing cell viability and exclusion of non-viable cells in flow cytometry. PI binds to double stranded DNA, but is excluded from cells with intact plasma membranes. PI should be analyzed in the PE channel when used as a counterstain for Annexin V FITC. 7-AAD can be used in place of PI when using Annexin V PE. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions. Choose propidium Iodide as a counterstain with Annexin V apoptosis assays.

Reactivity/Species
Human, Mouse, Rat

Vybrant™ Apoptosis Assay Kit #6, Biotin-X Annexin V/Alexa Fluor™ 350 Streptavidin/Propidium Iodide Invitrogen™

The Vybrant Apoptosis Assay Kit #6 uses the bright, blue-fluorescent Alexa Fluor 350 conjugate of streptavidin in conjunction with biotin-X annexin V to detect apoptotic cell populations either by flow cytometry or imaging. Alexa Fluor 350 streptavidin binds to biotin-X annexin V, which in turn binds to the phosphatidylserine on the surfaces of apoptotic cells. Necrotic cells are distinguished using red-fluorescent propidium iodide, which binds tightly to the nucleic acids in those cells. Propidium iodide does not penetrate the plasma membranes of live cells or early apoptotic cells. The kit contains an ample amount of each reagent for about 50 flow cytometric assays.

View a selection guide for all apoptosis assays for flow cytometry.

Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 & Propidium Iodide (PI) Invitrogen™

The Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 & Propidium Iodide is a flow cytometry kit used to measure early apoptosis by detecting phosphatidyl serine expression and membrane permeability.

View a selection guide for all apoptosis assays for flow cytometry.

Superior brightness
Unlike other Annexin V kits that have lower protein concentrations or purification levels, the Annexin V Alexa Fluor™ 488 conjugate is optimized for flow cytometry to provide the largest separation between apoptotic and live cells. The Alexa Fluor™ 488 dye is a superior green-fluorescent dye with a spectrum similar to fluorescein (FITC).

High binding efficiency
Annexin V conjugates are made from a highly purified cys-annexin, which leads to higher binding efficiency, resulting in highly accurate characterization of the apoptotic process.

Multi-parametric
Many publications require a minimum of two different ways to identify that cells are apoptotic. This multi-parametric kit detects phosphatidyl serine (PS) on the cytoplasmic surface of the cell membrane and membrane integrity using propidium iodide.

How it works
When cells are stained with Annexin V and propidium iodide, apoptotic cells expressing PS show green fluorescence, which can be detected in the FITC channel, and low red fluorescence. Dead or necrotic cells show bright red fluorescence and no green fluorescence, while live cells show no green or red fluorescence.

Vybrant™ Apoptosis Assay Kit #5, Hoechst 33342/Propidium Iodide Invitrogen™

The Vybrant Apoptosis Assay Kit #5 provides a rapid and convenient assay for apoptosis based on fluorescence detection of the compacted state of the chromatin in apoptotic cells. This kit contains ready-to-use solutions of blue-fluorescent Hoechst 33342 dye, which stains the condensed chromatin of apoptotic cells more brightly than the chromatin of nonapoptotic cells, and red-fluorescent propidium iodide dye, which stains dead cells.

FungaLight™ Yeast CFDA, AM/Propidium Iodide Vitality Kit Invitrogen™

The FungaLight™ Yeast CFDA, AM / Propidium Iodide Vitality Kit combines a cell permeable esterase substrate with a membrane integrity indicator to evaluate the vitality of yeast cells by flow cytometry or microscopy. The acetoxymethyl ester (AM) of the esterase substrate 5-carboxyfluorescein diacetate (CFDA) allows this reagent to permeant cell membranes Once inside the cell, the lipophilic blocking and diacetate groups are cleaved by nonspecific esterases, resulting in a fluorescent, charged form that leaks out of cells very slowly. In contrast, the membrane integrity indicator, propidium iodide penetrates yeast with damaged membranes. With an appropriate mixture of the CFDA, AM and propidium iodide stains, esterase-active yeast with intact cell membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.

Propidium Iodide ReadyProbes™ Reagent Invitrogen™

Propidium iodide is a commonly used dead-cell stain that emits red fluorescence when bound to DNA. Propidium iodide is also used as a counterstain in multicolor fluorescent techniques. With Propidium Iodide ReadyProbes® Reagent, we provide a room temperature-stable solution of classic DNA stain that comes in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells.

Also available: Propidium Iodide, Fluorspar grade at 12.5X concentration (13.2 µg/mL, 19.7 µM) in water with 1% DMSO.

See other Ready Probes ready-to-use imaging reagents and accessories ›

Features of Propidium Iodide ReadyProbes® Reagent:
• Ready-to-use liquid propidium iodide formulation
• Rapid staining of dead cells without wash steps
• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
• Stable at room temperature—keep handy at your work station or cell culture area

Classic Dead Cell Stain
Propidium iodide is a classic fluorescent dye used extensively for staining of dead cells (Figure 1). It exhibits a sufficiently large Stokes shift to allow simultaneous detection of nuclear DNA and FITC or Alexa Fluor® 488-labeled antibodies.

Packaged for Convenience
Propidium Iodide ReadyProbes® Reagent is supplied ready-to-use and can be stored right next to your flow cytometer or in your cell culture lab. A liquid formulation in an ultra-convenient dropper bottle (Figure 2) allows you to stain your cells whenever and wherever they are ready. Just add two drops per ml for optimally bright and distinct nuclear counter-staining.

Multiple Applications
Propidium iodide is membrane-impermeant and excluded from viable cells. It is therefore commonly used for identifying dead cells in a population. Its spectral properties, selectivity for dsDNA, and increased fluorescence when bound to DNA, make propidium iodide ideal for use with green (Alexa Fluor® 488, FITC, GFP) fluorophores in multicolor experiments. Because of its high affinity for DNA, it is also frequently used for counting cells, measuring apoptosis, and sorting cells based on DNA content.

Suggestions for Use
• In most cases, 2 drops/1×106 cells of Propidium Iodide ReadyProbes® Reagent and an incubation time of 15 to 30 minutes will produce bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
• When bound to nucleic acids, the fluorescence excitation maximum for propidium iodide is 535 nm and the emission maximum is 617 nm. Propidium iodide can be excited with a xenon or mercury-arc lamp or with the 488 line of an argon-ion laser. Generally, propidium iodide fluorescence is detected in the FL2 channel of flow cytometers.
• As a preferred red nuclear stain in dead cell analyses using flow cytometry, Propidium Iodide ReadyProbes® Reagent is ideal for use with antibody-based applications.

See other Molecular Probes® imaging tools and reagents.

Tali™ Apoptosis Kit - Annexin V Alexa Fluor™ 488 & Propidium Iodide Invitrogen™

The Tali® Apoptosis Kit enables identification of apoptotic cells and discrimination of apoptotic from necrotic and live cells in a population. The kit stains apoptotic cells with green Annexin V – Alexa Fluor® 488, stains necrotic cells with both red propidium iodide and green Annexin V – Alexa Fluor® 488, and does not stain live cells.

The Tali®Apoptosis Kit contains:

Annexin V – Alexa Fluor® 488 conjugate

• Annexin Binding solution
• Propidium Iodide (PI) solution

Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development. Inappropriately regulated apoptosis is implicated in disease states, such as Alzheimer’s disease and cancer. Apoptosis is distinguished from necrosis by characteristic morphological and biochemical changes, including compaction and fragmentation of the nuclear chromatin, shrinkage of the cytoplasm, and loss of membrane asymmetry.

Easily Discriminate Apoptotic from Necrotic Cells with Two-color Staining
In normal live cells, phosphatidyl serine (PS) is located on the cytoplasmic surface of the cell membrane. In apoptotic cells, however, PS is translocated from the inner to the outer surface of the plasma membrane, exposing it to the extracellular environment. The human anticoagulant Annexin V displays a high affinity for PS. Annexin V labeled with a fluorophore such as Alexa Fluor® 488 can be used to identify apoptotic cells by binding to the exposed PS.

The Tali® Apoptosis Kit includes a solution of propidium Iodide (PI). PI is a cell-impermeant fluorogenic DNA-binding dye used for years to identify necrotic cells. PI is impermeant to live cells, but easily enters dead cells where it binds to nucleic acids and becomes fluorescent.

After staining a cell population using the Tali® Apoptosis Assay Kit with Annexin V-Alexa Fluor® 488 and Propidium Iodide, apoptotic cells display green fluorescence, dead cells display red and green fluorescence and live cells show little or no fluorescence.

Propidium Iodide Ready Flow™ Reagent Invitrogen™

Propidium Iodide Ready Flow™ Reagent is a bright, easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays. This dye has excitation/emission maxima of 535/617 nm.

• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh. or pipette
• Rapid staining of dead cells without wash steps

Propidium Iodide Ready Flow Reagent is a commonly used dead-cell stain that emits red fluorescence when bound to DNA. Add 2 drops from the dropper bottle directly into 1 x 106 cells, incubate, and analyze.

Propidium iodide is membrane impermeant and therefore excluded from viable cells. It binds to DNA by intercalating between the bases, thereby binding both DNA and RNA. Propidium iodide is commonly excited with the 488 nm laser, but others such as the 532 and 561 nm lasers are also suited for PI excitation.

Chromatin Condensation & Membrane Permeability Dead Cell Apoptosis Kit with Hoechst 33342, YO-PRO™-1, and PI dyes, for flow cytometry Invitrogen™

This product detects apoptotic cells with changes in nuclear chromatin condensation and plasma membrane permeability, using three nucleic acid stains: UV-excitable Hoechst 33342, green fluorescent YO-PRO™ dye, and propidium iodide. The YO-PRO™ dye can enter apoptotic cells, whereas red-fluorescent propidium iodide (PI) cannot. Thus after staining with YO-PRO™-1 dye and PI, apoptotic cells show green fluorescence, and dead cells show primarily red fluorescence and some green fluorescence. Blue fluorescent Hoechst 33342 brightly stains the condensed chromatin of apoptotic cells and more dimly stains the normal chromatin of live cells. The staining pattern resulting from the simultaneous use of these three dyes makes it possible to distinguish normal, apoptotic and dead cell populations by flow cytometry or fluorescence microscopy.

View a selection guide for all apoptosis assays for flow cytometry.
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