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Propidium Iodide - 1.0 mg/mL Solution in Water (Invitrogen™)

Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.

PI binds to DNA by intercalating between the bases with little or no sequence preference. In aqueous solution, the dye has excitation/emission maxima of 493/636 nm. Once the dye is bound, its fluorescence is enhanced 20- to 30-fold, the fluorescence excitation maximum is shifted ~30–40 nm to the red and the fluorescence emission maximum is shifted ~15 nm to the blue, resulting in an excitation maximum at 535 nm and fluorescence emission maximum at 617 nm.

PI is widely used in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry.

Learn more about propidium iodide, and propidium iodide containing products

Propidium Iodide (Invitrogen™)

Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.

PI binds to DNA by intercalating between the bases with little or no sequence preference. In aqueous solution, the dye has excitation/emission maxima of 493/636 nm. Once the dye is bound, its fluorescence is enhanced 20- to 30-fold, the fluorescence excitation maximum is shifted ~30–40 nm to the red and the fluorescence emission maximum is shifted ~15 nm to the blue, resulting in an excitation maximum at 535 nm and fluorescence emission maximum at 617 nm.

PI is widely used in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry.

Learn more about propidium iodide, and propidium iodide containing products

Propidium Iodide - FluoroPure™ Grade (Invitrogen™)

Because it is manufactured at our ISO 9001—certified facilities in Eugene, Oregon, we can guarantee that FluoroPure grade propidium iodide is greater than or equal to 98% pure by HPLC.

Propidium Iodide ReadyProbes™ Reagent (Invitrogen™)

Propidium iodide is a commonly used dead-cell stain that emits red fluorescence when bound to DNA. Propidium iodide is also used as a counterstain in multicolor fluorescent techniques. With Propidium Iodide ReadyProbes® Reagent, we provide a room temperature-stable solution of classic DNA stain that comes in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells.

Features of Propidium Iodide ReadyProbes® Reagent:
• Ready-to-use liquid propidium iodide formulation
• Rapid staining of dead cells without wash steps
• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
• Stable at room temperature—keep handy at your work station or cell culture area

Classic Dead Cell Stain
Propidium iodide is a classic fluorescent dye used extensively for staining of dead cells (Figure 1). It exhibits a sufficiently large Stokes shift to allow simultaneous detection of nuclear DNA and FITC or Alexa Fluor® 488-labeled antibodies.

Packaged for Convenience
Propidium Iodide ReadyProbes® Reagent is supplied ready-to-use and can be stored right next to your flow cytometer or in your cell culture lab. A liquid formulation in an ultra-convenient dropper bottle (Figure 2) allows you to stain your cells whenever and wherever they are ready. Just add two drops per ml for optimally bright and distinct nuclear counter-staining.

Multiple Applications
Propidium iodide is membrane-impermeant and excluded from viable cells. It is therefore commonly used for identifying dead cells in a population. Its spectral properties, selectivity for dsDNA, and increased fluorescence when bound to DNA, make propidium iodide ideal for use with green (Alexa Fluor® 488, FITC, GFP) fluorophores in multicolor experiments. Because of its high affinity for DNA, it is also frequently used for counting cells, measuring apoptosis, and sorting cells based on DNA content.

Suggestions for Use
• In most cases, 2 drops/1×106 cells of Propidium Iodide ReadyProbes® Reagent and an incubation time of 15 to 30 minutes will produce bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
• When bound to nucleic acids, the fluorescence excitation maximum for propidium iodide is 535 nm and the emission maximum is 617 nm. Propidium iodide can be excited with a xenon or mercury-arc lamp or with the 488 line of an argon-ion laser. Generally, propidium iodide fluorescence is detected in the FL2 channel of flow cytometers.
• As a preferred red nuclear stain in dead cell analyses using flow cytometry, Propidium Iodide ReadyProbes® Reagent is ideal for use with antibody-based applications.

See other Molecular Probes® imaging tools and reagents.

eBioscience™ Propidium Iodide (Invitrogen™)

Propidium Iodide (PI) is a standard reagent used for assessing cell viability and exclusion of non-viable cells in flow cytometry. PI binds to double stranded DNA, but is excluded from cells with intact plasma membranes. PI should be analyzed in the PE channel when used as a counterstain for Annexin V FITC. 7-AAD can be used in place of PI when using Annexin V PE. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions. Choose propidium Iodide as a counterstain with Annexin V apoptosis assays.

Reactivity/Species
Human, Mouse, Rat

Propidium Iodide Ready Flow™ Reagent (Invitrogen™)

Propidium Iodide Ready Flow™ Reagent is a bright, easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays. This dye has excitation/emission maxima of 535/617 nm.

• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh. or pipette
• Rapid staining of dead cells without wash steps

Propidium Iodide Ready Flow Reagent is a commonly used dead-cell stain that emits red fluorescence when bound to DNA. Add 2 drops from the dropper bottle directly into 1 x 106 cells, incubate, and analyze.

Propidium iodide is membrane impermeant and therefore excluded from viable cells. It binds to DNA by intercalating between the bases, thereby binding both DNA and RNA. Propidium iodide is commonly excited with the 488 nm laser, but others such as the 532 and 561 nm lasers are also suited for PI excitation.

eBioscience™ Propidium Iodide Staining Solution (Invitrogen™)

Propidium Iodide (PI) is a ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. PI binds to double stranded DNA by intercalating between basepairs, but is excluded from cells with intact plasma membranes. PI can be used in FL3 for inviability exclusion, but should be analyzed in FL2 when used as a counterstain for FITC Annexin V.

Reported Application
Flow Cytometric Analysis

FxCycle™ PI/RNase Staining Solution (Invitrogen™)

FxCycle™ PI/RNase Staining Solution is used for flow cyotmetric analysis of DNA content in fixed cells. Propidium iodide (PI), a popular red-fluorescent stain, binds to DNA by intercalating between the bases with little or no sequence preference and with a stoichiometry of one dye per 4–5 base pairs of DNA. PI also binds to RNA, necessitating treatment with RNase to distinguish between RNA and DNA staining. This propidium iodide solution includes DNase-free RNase A and a permeablization reagent. It comes ready to use: simply add the staining solution to fixed cells, incubate, and acquire on a flow cytometer without washing.

View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry.