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Spectra™ Multicolor Broad Range Protein Ladder

Thermo Scientific Spectra Multicolor Broad Range Protein Ladder is a 4-color protein standard containing 10 prestained proteins (10 to 260kDa) for use in gel electrophoresis and Western blotting.

Features of Spectra Multicolor Broad Range Protein Ladder:

Size range—10 proteins spanning 10 to 260kDa
Multicolor—four different colors for unambiguous band-size assignment
Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
Sharp bands—color-coded bands of similar intensity for easy visualization
Quality tested—each lot evaluated by SDS-PAGE and Western blotting
Membrane-compatible—colored bands transfer to membranes for Western blotting

Includes:
• Dye-stained proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol.

Applications:
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after Western blotting
• Sizing of proteins on SDS-polyacrylamide gels and Western blots

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Four different chromophores (blue, orange, green, pink) are bound to the different component proteins, producing a brightly colored ladder with an easy-to-remember pattern.

Please see Cat. No. 26634 for more information about this product.

Spectra™ Multicolor High Range Protein Ladder (Thermo Scientific™)

Thermo Scientific Spectra Multicolor High Range Protein Ladder is a mixture of eight (8) blue-, green-and orange-stained proteins (40 to 300kDa) for use as size standards for high-MW proteins in gel electrophoresis and Western blotting.

Features of Spectra Multicolor High Range Protein Ladder:

Size range—eight proteins spanning 40 to 300kDa
Multicolor—three different colors for unambiguous band-size assignment
Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
Sharp bands—color-coded bands of similar intensity for easy visualization
Quality tested—each lot evaluated by SDS-PAGE and Western blotting
Membrane-compatible—colored bands transfer to membranes for Western blotting

Includes:
• Dye-stained proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol.

Applications:
• Monitoring of protein migration during SDS-PAGE
• Verifying Western transfer efficiency
• Approximate sizing of proteins on SDS-polyacrylamide gels and Western blots
• Locating a protein of interest for excision from an unstained preparative gel

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Three different chromophores (blue, orange, green) are bound to the different component proteins, producing a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required.

Spectra™ Multicolor Low Range Protein Ladder (Thermo Scientific™)

Thermo Scientific Spectra Multicolor Low Range Protein Ladder is a mixture of six proteins and synthetic peptides (1.7 to 40kDa) for use as size standards for peptides and small proteins in gel electrophoresis and Western blotting.

Features of Spectra Multicolor Low Range Protein Ladder:

Size range—six proteins and peptides spanning 1.7 to 40kDa
Multicolor—three different colors for unambiguous band-size assignment
Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
Sharp bands—color-coded bands of similar intensity for easy visualization
Quality tested—each lot evaluated by SDS-PAGE and Western blotting
Membrane-compatible—colored bands transfer to membranes for Western blotting

Includes:
• Dye-stained proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol.

Applications:
• Monitoring of protein migration during SDS-PAGE
• Verifying Western transfer efficiency
• Approximate sizing of proteins on SDS-polyacrylamide gels and Western blots
• Locating a protein of interest for excision from an unstained preparative gel

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Three different chromophores (blue, orange, green) are bound to the different component proteins, producing a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required.