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GelCode™ Blue Safe Protein Stain (Thermo Scientific™)

Thermo Scientific GelCode Blue Safe Protein Stain is a fast and sensitive coomassie-dye reagent that provides one-step ultrasensitive detection of proteins in polyacrylamide gels and is formulated to avoid hazardous shipping costs.

Features of GelCode Blue Safe Protein Stain:

Sensitive and fast—detect down to 9 ng of protein per band in 15 minutes
Safe—non-corrosive to skin, non-flammable and safe to ship and store until activated
Convenient—water washes only; no acid-fixative or methanol destain solutions required
Versatile—suitable for staining 1D and 2D polyacrylamide gels, nitrocellulose and PVDF membranes (multiple protocols provided in the instructions)
Compatible—useful for qualitative visualization, quantitative densitometry, and downstream excision and analysis by mass spectrometry
Easy to use—add and dissolve activator crystals before first use, then simply add reagent to stain gels
Stable—store non-activated or activated stain at room temperature for up to one year

GelCode Blue Safe Protein Stain is a coomassie G-250 dye-based reagent for staining protein on polyacrylamide gels and membranes. GelCode Blue Safe Stain provides considerable sensitivity, allowing for the detection down to 9 ng of protein. This protein-specific stain allows bands to be viewed directly on a gel or membrane during staining and is compatible with mass spectrometry applications. The staining process is simple and flexible, usually requiring a 15-minute incubation of the gel or sample with the GelCode Blue Safe Protein Stain, followed by a simple water wash to yield blue protein bands and a clear background.

Because the supplied Activator Crystals are packaged and shipped dry in a separate bottle, GelCode Blue Safe Protein Stain is classified as nonhazardous under the U.S. Department of Transportation (DOT) shipping requirements.

For proper protein staining, the Activator Crystals must be dissolved in the staining reagent before use. Add the entire contents of the Activator Crystals bottle into the GelCode Blue Safe Protein Stain bottle. Dissolve the crystals by mixing end-over-end for 1 minute, and then allow it to remain at room temperature for 5 minutes before use. Store the activated stain at room temperature.

Novex™ Reversible Membrane Protein Stain Kit (Invitrogen™)

Novex® Reversible Protein Stain is a highly sensitive stain used for determining protein transfer efficiency after blotting. Quantitative and completely reversible, this stain is compatible for use with both nitrocellulose (NC) and polyvinylidene difluoride (PVDF) membranes.

Pierce™ Reversible Protein Stain Kit for Nitrocellulose Membranes (Thermo Scientific™)

The Thermo Scientific Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes is a rapid and sensitive alternative to Ponceau S stain for protein detection on nitrocellulose membrane after transfer from polyacrylamide gels.

Features of the Reversible Protein Stain Kit for Nitrocellulose Membranes:

Better than Ponceau S—more sensitive, easier to document, permanent until reversed (does not fade)
Sensitive—high-avidity, total-protein stain; lower limit of detection equals 25 to 50 ng per band
Specific—detects only protein; does not bind or interact with other electrophoresis or sample components
Rapid—stain in less than 5 minutes; completely erase and reverse staining in less than 15 minutes
Stable—components are stable at room temperature, saving refrigerator space and eliminating equilibration steps

This kit for membrane staining uses a nondestructive, reversible, reliable and sensitive method to stain and detect proteins on nitrocellulose membranes. The kit is a superior alternative to Ponceau S stains for evaluating the efficiency of protein transfer following SDS-PAGE and before immunoblotting. The lower limit of detection with this method is 25 to 50 ng per band (at least five times more sensitive than traditional Ponceau S staining). The staining protocol is simple, quick and results in turquoise-blue bands that do not fade and are easily photographed for future reference. The stain can be easily reversed in less than 15 minutes. Subsequent western blot detection is unaffected because the stain does not alter the protein and is completely removed.

The Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes has excellent avidity for a broad range of proteins, resulting in low protein-to-protein variability and enabling most proteins to be detected at very low levels (25 to 50ng per band). Pierce Membrane Stain is superior to other protein stains available for nitrocellulose membranes. For example, Ponceau S is less sensitive and results in red bands that easily fade and are difficult to photograph. Coomassie dye is a sensitive stain, but it permanently binds to proteins and can interfere with western blotting.

The kit uses a dye that has a high affinity for protein but does not permanently bind. The stain has minimal nonspecific interactions with the membrane surface and protein-transfer reagents, and the technique is compatible with general western blot systems. The treated membrane does not interfere with conventional chemiluminescent or chromogenic detection using HRP and alkaline phosphatase substrates. In addition, the stain is compatible with N-terminal sequence analysis of proteins excised and eluted from membrane.

Related Products
Pierce™ Reversible Protein Stain Kit for PVDF Membranes

Pierce™ Reversible Protein Stain Kit for PVDF Membranes (Thermo Scientific™)

The Thermo Scientific Pierce Reversible Protein Stain Kit for PVDF Membranes is a rapid and sensitive alternative to Ponceau S stain for protein detection on PVDF membrane after transfer from polyacrylamide gels.

Features of the Reversible Protein Stain Kit for PVDF Membranes:

Better than Ponceau S—more sensitive, easier to document, permanent until reversed (does not fade)
Sensitive—high-avidity, total-protein stain; lower limit of detection equals 25 to 50 ng per band
Specific—detects only protein; does not bind or interact with other electrophoresis or sample components
Rapid—stain in less than 30 minutes; completely erase and reverse staining in less than 15 minutes
Stable—components are stable at room temperature, saving refrigerator space and eliminating equilibration steps

This kit for membrane staining uses a nondestructive, reversible, reliable and sensitive method to stain and detect proteins on PVDF membranes. The kit is a superior alternative to Ponceau S stains for evaluating the efficiency of protein transfer following SDS-PAGE and before immunoblotting. The lower limit of detection with this method is 25 to 50 ng per band (at least five times more sensitive than traditional Ponceau S staining). The staining protocol is simple, quick and results in turquoise-blue bands that do not fade and are easily photographed for future reference. The stain can be easily reversed in less than 15 minutes. Subsequent western blot detection is unaffected because the stain does not alter the protein and is completely removed.

The Pierce Reversible Protein Stain Kit for PVDF Membranes has excellent avidity for a broad range of proteins, resulting in low protein-to-protein variability and enabling most proteins to be detected at very low levels (25 to 50ng per band). Pierce Membrane Stain is superior to other protein stains available for PVDF membranes. For example, Ponceau S is less sensitive and results in red bands that easily fade and are difficult to photograph. Coomassie dye is a sensitive stain, but it permanently binds to proteins and can interfere with western blotting.

The kit uses a dye that has a high affinity for protein but does not permanently bind. The stain has minimal nonspecific interactions with the membrane surface and protein-transfer reagents, and the technique is compatible with general western blot systems. The treated membrane does not interfere with conventional chemiluminescent or chromogenic detection using HRP and alkaline phosphatase substrates. In addition, the stain is compatible with N-terminal sequence analysis of proteins excised and eluted from membrane.

Related Products
Pierce™ Reversible Protein Stain Kit for Nitrocellulose Membranes
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Krypton™ Fluorescent Protein Stain (Thermo Scientific™)

Thermo Scientific Krypton Fluorescent Protein Stain enables sensitive visualization with fluorescence imagers (520nm excitation; 580nm emission) of proteins separated in polyacrylamide gels or transferred to membranes.

Features of Krypton Fluorescent Protein Stain:

Intense fluorescent stain—excitation and emission maxima of 520nm and 580nm, respectively (compatible with 532nm excitation light sources and 580 to 600nm emission filters)
Instrument-compatible—effective with popular laser-based and filter-based fluorescence imagers
Broad dynamic range—linear quantitative range spans three to four orders of magnitude Sensitive—detect as little as 0.25ng protein with the standard protocol (approx. 2.5 hours); at least as sensitive as fluorescent stains from other suppliers
Fast—standard (2.5 hours) and rapid (30 minutes) protocols are significantly faster than procedures of other popular fluorescent stains
Versatile—compatible with proteomics workflows involving downstream mass spectrometry
Compact—supplied as a 10X solution to save storage space; just dilute with water before use

This fluorescent protein gel stain is for bright yellow-orange fluorescent detection of proteins separated by 1D or 2D polyacrylamide gel electrophoresis (SDS-PAGE). Krypton Stain uses a protein-binding dye with fluorescence properties (Ex/Em = 520/580nm) suitable for visualization with a variety of standard laser-based and filter-based fluorescence imagers and documentation systems. Proteins are detectable at concentrations greater than 0.25ng per band in a typical mini-gel lane (greater than 2ng for the rapid staining protocol). Krypton Stain provides high signal intensity, exhibits minimal protein-to-protein variation, has an excellent quantitative dynamic range and is compatible with subsequent destaining and analysis by mass spectrometry.

For best results, detect bands using visible laser-based imagers equipped with a 532nm laser light source. Although 580nm-filters are optimal for emission, 600nm-filters are also compatible. Stained gels can be imaged on any platform with the respective excitation and emission filters.

Imperial™ Protein Stain (Thermo Scientific™)

Thermo Scientific Imperial Protein Stain is a ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers consistent nanogram-level detection of proteins in polyacrylamide electrophoresis gels.

Features of Imperial Protein Stain:

Sensitive—detects less than 3ng protein per band with the enhanced protocol (3 hours)
Fast—ready-to-use reagent detects less than 6ng protein per band in just 20 minutes
Robust—highly consistent, reproducible protein staining technique
High contrast—intense purple bands are easier to photograph or scan than typical coomassie blue stains
Versatile—compatible with downstream mass spectrometry analysis and protein sequencing
Convenient—water washes only; no acid-fixative or methanol destaining solutions required
Stable—1-year room-temperature stability ensures consistent performance and saves refrigerator space
Flexible—adjust staining and washing times to meet time- and sensitivity-requirements

Imperial Protein Stain is a coomassie dye reagent for detection of protein bands in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and 2D gels. The stain is a unique formulation of coomassie brilliant blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercial stains. Staining results in intensely colored protein bands that are easy to photograph and document with gel imagers. This reagent is one of the most sensitive colorimetric stains available, easily detecting 3 to 6 nanograms of protein per band. The Imperial Protein Stain protocol uses simple water-wash steps rather than methanol/acetic acid fixation and destaining, which saves valuable preparation time and minimizes reagent costs.

Imperial Protein stain is a unique formulation of Coomassie Brilliant Blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercially available coomassie-based stains. The easy-to-follow protocol is flexible to meet demanding time and sensitivity requirements. The use of Imperial Protein Stain does not result in the problems (e.g., staining consistency) that can be associated with other coomassie G-250 stain preparations. In addition to faster protein band development and improved sensitivity over standard coomassie G-250 stains, Imperial Protein Stain does not require methanol/acetic acid fixation and destaining, saving valuable preparation time and minimizing reagent cost.

TC-FlAsH™ II In-Cell Tetracysteine Tag Detection Kit (Green Fluorescence), for live-cell imaging (Invitrogen™)

TC-FlAsH™ II In-Cell Tetracysteine Tag Detection Kit contains an expression tag-based fluorescence labeling reagent for live-cell labeling. Mammalian cell lines expressing a protein fused to a tetracysteine tag (CCPGCC) can be labeled with the green-fluorescent FlAsH-EDT2 reagent contained in the kit. The tagged protein is fluorescent only when the labeling reagent is added. BAL wash buffer replaces the previously supplied Disperse Blue 3 and EDT-based wash buffer as an olfactorily more agreeable reagent that yields superior signal to noise.


The kit contains FlAsH-EDT2 labeling reagent (store at -20°C, protected from light) and BAL wash buffer (stored at 4°C). When stored as directed, the kit is stable for 6 months.

Lumio™ Green In-Cell Detection Kit (Invitrogen™)

Lumio™ Technology is designed for simple fluorescent detection of recombinant protein. The Lumio™ tag is a small (six amino acid) sequence that binds a fluorescent substrate, allowing you to visually detect protein localization in mammalian cells. The Mammalian Lumio™ Gateway® vectors (Figure 1) offer the following features:

• Lumio™ tag for accurate in vitro and in vivo protein detection
• CMV promoter for high-level constitutive expression
• tR sites for efficient recombination with Gateway® entry clones
• Blasticidin resistance gene for fast, efficient selection

Use Mammalian Lumio™ Gateway® vectors for reliable and consistent protein expression, detection, and localization in mammalian cells (Figure 2). Vectors are available for generating protein with an N- and C-terminal Lumio™ fusion tag. In addition, each Mammalian Lumio™ Gateway® Kit includes a Lumio™ Green, Red, or Dual In-Cell Detection Kit. The Dual In-Cell Detection Kit is ideal for doing two color "pulse-chase" experiments to examine protein localization over time.

TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit (Red Fluorescence), for live-cell imaging (Invitrogen™)

TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit contains an expression tag-based fluorescence labeling reagent for live-cell labeling. Mammalian cell lines expressing a protein fused to a tetracysteine tag (CCPGCC) can be labeled with the red-fluorescent ReAsH-EDT2 reagent contained in the kit. The tagged protein is fluorescent only when the labeling reagent is added. BAL wash buffer replaces the previously supplied Disperse Blue 3 and EDT-based wash buffer as an olfactorily more agreeable reagent that yields superior signal to noise.


The kit contains ReAsH-EDT2 labeling reagent (store at -20°C, protected from light) and BAL wash buffer (stored at 4°C). When stored as directed, the kit is stable for 6 months.

TC-FlAsH™ TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit, with Mammalian TC-Tag Gateway™ Expression Vectors (Green Fluorescence) (Red Fluorescence) (Invitrogen™)

TC-FlAsH™ TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit is an expression tag-based fluorescence labeling system for live-cell labeling. The expression construct is created when the Gateway entry clone (containing the gene of interest) is recombined with one of two destination vectors (pcDNA6.2/nTC-Tag-DEST for N-terminal tagging or pcDNA6.2/cTC-Tag-DEST for C-terminal tagging). The expression construct is then used to transform the host strain. The protein can be detected either with green-fluorescent FlAsH-EDT2 reagent or red-fluorescent ReAsH-EDT2 reagent. The tag is very small (6 amino acids) and the protein of interest is fluorescent only when the labeling reagent is added. BAL wash buffer replaces the previously supplied Disperse Blue 3 and EDT-based wash buffer as an olfactorily more agreeable reagent that yields superior signal to noise.

The kit contains FlAsH-EDT2 labeling reagent, ReAsH-EDT2 labeling reagent, BAL wash buffer, pcDNA6.2/cTC-Tag-DEST, pcDNA6.2/nTC-Tag-DEST, and pcDNA6.2/nTC-Tag-p64 Control Plasmid. BAL wash buffer should be stored at 4°C, and the rest of the kit stored at -20°C, protected from light. The kit is stable for 6 months when stored correctly.

Pro-Q™ Emerald 300 Glycoprotein Gel Stain Kit, with SYPRO™ Ruby Protein Gel Stain (Invitrogen™)

Molecular Probes' proprietary Pro-Q Emerald 300 and Pro-Q Emerald 488 Glycoprotein Stain Kits provide the most advanced technology available for detection of glycoproteins in gels and on blots. Gel staining is rapid and very sensitive. In less than three hours, it is possible to detect as little as 300 pg of glycoprotein per band, depending on the degree of glycosylation, making these stains at least 50-fold more sensitive than standard fuchsin staining.

Coomassie Fluor™ Orange Protein Gel Stain (Ready-To-Use Solution) (Invitrogen™)

Molecular Probes' proprietary Coomassie Fluor Orange protein gel stain provides fast, simple, sensitive staining of proteins separated by SDS-PAGE. After electrophoresis, the gel is stained, rinsed and photographed. Staining is complete in less than an hour – no separate fixation or destaining steps are required, and there is no risk of overstaining the gel. Stained proteins can be visualized using a standard 300 nm UV transilluminator or a laser-based scanner. Coomassie Fluor Orange protein gel stain is also available in a 5 liter size (Cat. no. C-33251).

Pierce™ 6xHis Protein Tag Stain Reagent Set (Thermo Scientific™)

The Thermo Scientific Pierce 6xHis Protein Tag Stain Reagent Set is a fluorescent stain for preferential detection of histidine-tagged (His-tagged) recombinant fusion proteins in protein polyacrylamide gels.

The unique fluorescent stain specifically detects histidine-tagged fusion proteins that have been electrophoresed in polyacrylamide gels (e.g., SDS-PAGE). With this stain, expression of His-tagged recombinant proteins can be assessed directly in-gel, eliminating membrane transfer and Western blotting steps that are typically used to confirm purity and yield of tagged proteins. Although the lower limit of detection depends on which UV lamp (300nm) and detection equipment (CCD camera best) are used, the stain is convenient for in-process assessment of His-tagged protein production and is compatible with subsequent staining with coomassie dye and other total protein stains.

Features of the 6xHis Protein Tag Stain Reagent Set:

Two- to three-times faster than Western blotting—get results sooner and save hours of valuable research time
Detects directly on the gel—can eliminate the need for a membrane transfer and Western blot step for the detection of at least 0.2 µg of 6xHis-tagged protein
Ready-to-use, two-reagent formula—no mixing, no diluting and no fuss; guarantees a simple-to-perform, mistake-free detection protocol
Specific fluorescent detection of 6xHis-tagged proteins—see only what you want to see (CCD camera recommended for low abundance proteins; UV-transilluminator for abundant proteins)
Compatible with double-staining—follow His-tagged protein staining with Thermo Scientific GelCode Blue Stain Reagent for a total protein profile determination

Includes:
• Stain and developer solutions (500 mL each)

Requires:
• UV lamp source (300nm) and CCD camera

SYPRO™ Orange Protein Gel Stain (5,000X Concentrate in DMSO) (Invitrogen™)

SYPRO® Orange Protein Gel Stain is a sensitive, ready-to-use fluorescent stain for proteins in 1D gels. It offers many advantages over Coomassie® Blue staining:

• Fast, one-step staining protocol requiring no destaining
• Linear range over three orders of magnitude
• Very little protein-to-protein variation in staining

Using SYPRO® Orange Protein Gel Stain
Stained proteins can be viewed with a standard UV or blue-light transilluminator or with a laser scanner. For optimal sensitivity with Polaroid film, we recommend using the SYPRO® Photographic Filter (S6656).

SYPRO™ Ruby Protein Gel Stain (Invitrogen™)

SYPRO® Ruby Protein Gel Stain is a highly sensitive, ready-to-use fluorescent stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). SYPRO® Ruby stain is ideal for use in 1D and 2D PAGE. The sensitivity of SYPRO® Ruby Gel Stain is as good as or better than the best silver staining techniques, and this stain offers several advantages over silver staining:

• Simple staining procedure—no destaining or timed steps required
• Linear quantitation range over three orders of magnitude
• Compatibility with mass spectrometry and microsequencing

Stained proteins can be viewed with a standard UV or blue-light transilluminator or with a laser scanner. For optimal sensitivity with Polaroid film, use of the SYPRO® Photographic Filter (Cat. No. S-6656) is recommended.