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Pierce™ Iodoacetamide, Single-Use (Thermo Scientific™)

Thermo Scientific Pierce Iodoacetamide is a sulfhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterization and peptide mapping. Alkylation with iodoacetamide after cystine reduction results in the covalent addition of a carbamidomethyl group (57.07 Da) and prevents the formation of disulfide bonds. Reducing agents added after alkylation will react with excess iodoacetamide.

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Pierce™ Iodoacetic Acid

Pierce™ NEM (N-ethylmaleimide) (Thermo Scientific™)

Thermo Scientific Pierce N-Ethylmaleimide (NEM) is a small compound that forms stable, covalent thioether bonds with sulfhydryls (e.g., reduced cysteines), enabling them to be permanently blocked to prevent disulfide bond formation.

Features of N-Ethylmaleimide (NEM):

• Permanently block sulfhydryls to prevent disulfide bond formation.
• Monitor the reaction by measuring the decrease in absorbance at 300nm
• Block sulfhydryl-containing reagents that interfere in enzyme assays

N-Ethylmaleimide (NEM) is an alkylating reagent that reacts with sulfhydryls. At pH 6.5-7.5, the maleimide reaction is specific for sulfhdryls; however, at pH values greater than 7.5, reactivity with amino groups occurs. Maleimide groups react with sulfhydryls by nucleophilic attack of the thiolate anion on one of the carbons of the double bond. When sufficient sulfhydryls have been blocked, the reaction can be monitored by measuring the decrease in absorbance at 300nm as the double bond becomes a single bond. The resulting thioether group is non-reversible and terminates in an ethyl group, blocking or capping the sulfhydryl.

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Pierce™ MMTS (methyl methanethiosulfonate)

Pierce™ Chloroacetamide, No-Weigh™ Format (Thermo Scientific™)

Thermo Scientific Pierce Chloroacetamide is a sulfhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterization. Alkylation with chloroacetamide after cysteine reduction results in the covalent addition of a carbamidomethyl group (57.07 Da) and prevents the formation of disulfide bonds. Reducing agents added after alkylation will react with excess chloroacetamide.

The No-Weigh format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. The format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent.

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Pierce Iodoacetic Acid