Detection Method


Storage Requirements


Products

Qubit™ dsDNA BR Assay Kit Invitrogen™

The Qubit dsDNA BR Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentration from 100 pg/µl–1,000 ng/µl. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.

Which product to choose for dsDNA BR (Broad Range) quantitation?
• For 1–20 samples: use this Qubit dsDNA BR Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT dsDNA BR Assay Kit with microplate reader

Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.

Qubit™ 1X dsDNA HS Assay Kit Invitrogen™

The Qubit 1X dsDNA HS (High-Sensitivity) Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. The '1X' dsDNA assay kit provides reagent and buffer in a ready-to-use formulation. Simply add your sample (any volume between 1 µL and 20 µL is acceptable), add the Qubit 1X dsDNA HS reagent, and read the concentration using a Qubit 2.0, 3, 4, or Flex fluorometer. Common contaminants such as salts, free nucleotides, solvents, detergents, and protein are well-tolerated in the assay.

Notes:
1. The Qubit 1X dsDNA HS Assay works on Qubit 2.0, 3, 4, and Flex fluorometers. It will not work on the original Qubit 1.0 Fluorometer. Download the MyQubit .qbt file for the Qubit 2.0 or 3 fluorometer.
2. 500-µL thin-walled tubes displaying low auto-fluorescence (Cat. No. Q32856) are required but not included.

Qubit™ ssDNA Assay Kit Invitrogen™

The Qubit ssDNA Assay Kit is designed specifically for use with the Qubit Fluorometer, but may be used with any fluorometer or fluorescence plate reader. The Qubit® ssDNA Kit is ideal for quantitating single-stranded DNA or oligonucleotides. However, it is not specific for single-stranded DNA. This assay kit will also detect double-stranded DNA and RNA but it will not detect contaminating protein or nucleotides. The assay is designed to be accurate for initial sample concentrations from 50 pg/µL to 200 ng/µL providing an assay range of 1–200 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.

Which product to choose for ssDNA quantitation?
• For 1–20 samples: use this Qubit ssDNA Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT OliGreen ssDNA Assay Kit with microplate reader

Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.

Qubit™ RNA IQ Assay Kit Invitrogen™

The Qubit RNA IQ Assay Kit provides a fast, simple method to check whether an RNA sample has degraded using the Qubit 4 Fluorometer (required). The assay utilizes two unique dyes: one that binds to large, intact and/or highly structured RNA (mRNA, tRNA, rRNA), and another that selectively binds to small and/or, degraded RNA. Together they enable you to quickly assess the quality and integrity of an RNA sample.

To use, simply add your samples to the RNA IQ working solution, then measure on the Qubit 4 Fluorometer. Results are presented as an RNA IQ number (RNA IQ#) that indicates the RNA sample integrity and quality, and also as a calculated percent of large and small RNA in the sample. The RNA IQ# is a value from 1 to 10, similar to other RNA quality scores, where a small number indicates that the sample is comprised of mainly small RNA and a larger number indicates that the sample consists of mainly large RNA or RNA with tertiary structure.

Solution-based compared to electrophoresis methods
The RNA IQ assay results correlate well with electrophoretic methods. The primary difference between the solution-based RNA IQ assay and traditional electrophoresis-based techniques is the amount of time it takes to ascertain whether an RNA sample has degraded. Gels are considered easy to perform, but still take time to run. Microfluidic-based methods not only take time to obtain results, but often have lengthy procedures to prepare the samples. Gel or microfluidic-based electrophoresis is still recommended to obtain fragment size information or size distribution of the RNA sample.

Notes:
1. The Qubit RNA IQ assay will only work on the Qubit 4 Fluorometer. It will not work on the Qubit, Qubit 2.0, or Qubit 3 fluorometers.
2. 500 µL thin-walled PCR tubes (Cat. No. Q32856) are required but not included.

Qubit™ RNA HS Assay Kit Invitrogen™

The Qubit RNA HS Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of low-abundance RNA samples. The assay is highly selective for RNA and will not quantitate DNA, protein, or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein, are well-tolerated in the assay. The assay kit is designed to be accurate for RNA sample concentrations between 250 pg/µL and 100 ng/µL. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using the Qubit Fluorometer.

Which product to choose for RNA HS (High Sensitivity) quantitation?
• For 1–20 samples: use this Qubit RNA HS Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT RNA HS Assay Kit with microplate reader

Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.

Qubit™ RNA XR Assay Kit Invitrogen™

The Qubit RNA XR (Extended Range) Assay Kit, designed for use with the Qubit 4 and Qubit Flex fluorometers, provides an accurate and selective method for the quantitation of high-abundance RNA samples. The assay is highly selective for RNA and does not quantitate DNA, protein, or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents, and protein, are well-tolerated in the assay. The assay kit is designed to be accurate for initial RNA sample concentrations of 10 to 10,000 ng/μL depending on sample volume, providing a linear detection range of 200−10,000 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add the sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using a Qubit 4 or Qubit Flex fluorometer.

Notes:
1. Use thin-wall, clear, 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 fluorometer and 8 x 200-µL tube strips (Cat. No. Q33252) for the Qubit Flex fluorometer.
2. Qubit 4 and Qubit Flex fluorometers may require a firmware update to run the RNA XR Assay.

Qubit™ Fluorometer USB Flash Drive Invitrogen™

The Qubit Fluorometer USB Flash Drive is designed for the transfer of data from the Qubit Fluorometer. Only the Qubit 2.0, 3, and 4 fluorometers have an available USB port. Data exported from the Qubit Fluorometer are stored in CSV format and can be opened by programs such as Microsoft Excel.

QuantStudio™ 3 Real-Time PCR System, MiniAmp™ Plus Thermal Cycler, and Qubit Fluorometer Package Applied Biosystems™

This is a package consisting of a QuantStudio 3 Real-Time PCR System with laptop, MiniAmp Plus Thermal Cycler, Qubit 4 Quantitation Starter Kit (including fluorometer), and reaction plates/films.

QuantStudio 3 Real-Time PCR System
The Applied Biosystems QuantStudio 3 Real-Time PCR System is designed for users who need an affordable, easy-to-use real-time PCR system that doesn't compromise performance and quality. The simplified Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio 3 system provides access to your data wherever and whenever you want. Using proven OptiFlex technology (featuring four coupled channels and white LED) and featuring three independent Veriflex temperature zones, the QuantStudio 3 system enables improved data accuracy and sensitivity for a broad range of genomic applications.

Learn more about the QuantStudio 3 system >

MiniAmp Plus Thermal Cycler
The Applied Biosystems MiniAmp Plus Thermal Cycler is a compact, accurate, and economical thermal cycler designed to fit your lab’s basic thermal cycling needs. Innovations like a responsive color touch-screen and VeriFlex Blocks temperature-control technology enable simple, accurate optimization. The MiniAmp Plus Thermal Cycler can be accessed through the Thermo Fisher Cloud, giving you the freedom to design and securely upload your methods from any mobile device or desktop computer.

Learn more about the MiniAmp Plus Thermal Cycler ›

Qubit 4 Quantitation Starter Kit
The Qubit 4 Fluorometer is the latest version of the popular benchtop fluorometer designed to accurately measure DNA, RNA, and protein quantity. It also easily measures RNA integrity and quality. The easy-to-use touch screen menus make it easy to select and run the assays you need, with results displayed in just a few seconds. The Qubit 4 Quantitation Starter Kit includes:
• One Qubit 4 Fluorometer (Cat. No. Q33226)
• Qubit Assay Tubes (set of 500) (Cat. No. Q32856)
• Qubit 1X dsDNA HS Assay Kit, 100 assays
• Qubit dsDNA BR Assay Kit, 100 assays (Cat. No. Q32850)
• Qubit RNA IQ Assay Kit, 75 assays (Cat. No. Q33221)
• Qubit RNA HS Assay Kit, 100 assays (Cat. No. Q32852)
• Qubit RNA XR Assay Kit, 100 assays (Cat. No. Q33223)
• Qubit Protein Assay Kit, 100 assays (Cat. No. Q33211)

Learn more about the Qubit 4 Fluorometer and this starter kit ›

MicroAmp Optical 96-Well Reaction Plate with Barcode & Optical Adhesive Films
The Applied Biosystems MicroAmp Optical 96-Well Reaction Plate is optimized to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification. This plate, constructed from a single rigid piece of polypropylene in a 96-well format, is compatible with Applied Biosystems 96-well real-time PCR systems and thermal cyclers.

The included MicroAmp Optical Adhesive Films reduce the chance of well-to-well contamination and sample evaporation when applied to the microplates.

Learn more about these plates and films ›

NanoDrop™ One Spectrophotometer with WiFi & Qubit™ 4 Fluorometer Invitrogen™

For DNA, RNA or protein samples that are 100% pure, the results obtained on the Thermo Scientific™ NanoDrop™ One Microvolume UV-Vis Spectrophotometer and the Invitrogen Qubit 4 Fluorometer would be identical for samples within their dynamic ranges. However, few samples are 100% pure.

As a bundled offering, the NanoDrop One Microvolume UV-Vis Spectrophotometer and the Qubit 4 Fluorometer together allow you to obtain the most complete information about the concentration and quality of your DNA, RNA or protein samples—helping to prevent costly troubleshooting downstream. While both the NanoDrop One spectrophotometer and the Qubit 4 fluorometer offer accurate measurements of DNA, RNA and protein samples, each also has its unique advantages.

NanoDrop One Spectrophotometer
Cited in more than 30,000 publications, the NanoDrop UV-Vis microvolume spectrophotometers have fundamentally changed the way scientists evaluate nucleic acid and protein samples by minimizing the need for large sample volumes.

The next generation NanoDrop One Microvolume UV-Vis Spectrophotometer utilizes innovative Thermo Scientific Acclaro™ Sample Intelligence technology that identifies contaminants, provides corrected concentration values and helps you understand the quality of your sample before you use it in downstream applications.

Qubit 4 Fluorometer
The Qubit 4 Fluorometer is a cutting-edge benchtop device that accurately measures DNA, RNA, and protein quantity, and now also RNA integrity and quality, using the highly sensitive Qubit assays.

For all Qubit assays, the concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result. The easy-to-use touchscreen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.

Comparison of the NanoDrop One spectrophotometer and Qubit 4 Fluorometer

NanoDrop One Spectrophotometer

 

Qubit 4 Fluorometer

Fast and simple. Allows for direct measurement of purified samples with no dilutions required.

Overview

Sensitive and specific. Accurately measures concentration whether dsDNA, ssDNA, total RNA, microRNA or protein— even in the presence of contaminants.

UV absorbance

Technology

Fluorescence

Pipette sample onto pedestal; measure.

Workflow

Mix sample with reagents, incubate for 2–5 minutes, read.

Routine quantitation and qualification of DNA, RNA and proteins

Applications

Routine samples and with very dilute or difficult to process samples, when higher measurement sensitivity is required or when nucleic acid contaminants are suspected (e.g., DNA in RNA, dNTPs)

RNA sample quality

0.21–27,500

dsDNA quantitation range (ng/ul)

0.01–1,000

Acclaro Sample Intelligence technology identifies presence of contaminants such as protein or guanidine; provides corrected analyte concentration value.

Sample qualification (purity)

Unique fluorescent dyes enable differentiation between nucleic acid types (e.g., DNA vs. RNA) without any sample pre-treatment.

RNA IQ assay enables fast and facile detection of intact vs degraded in <5 seconds per sample

Selection of pre-programmed applications

  • Direct A280 quantitation with Protein Editor
  • A205 peptide bond absorbance
  • Colorimetric protein assays: Bradford, BCA, Lowry and Thermo Scientific Pierce™ 660 nm assay

Protein Quantitation

Qubit assays exhibit minimal protein-to-protein variability and are resistant to most contaminants, including salts, reducing agents (e.g., DTT, b-mercaoptoethanol), DNA or amino acids, but not detergents.

1dsDNA quantitation range for NanoDrop One is 0.2 ng/µL with cuvette and 2.0 ng/µL without.

Key instrument features include:

NanoDrop One Microvolume UV-Vis Spectrophotometer
• Fast and easy sample evaluation of nucleic acids and proteins with “pipette, measure, clean” workflow and no sample carryover
• Modern stand-alone design with local control and high-resolution touchscreen interface, which saves bench space
• Accurate measurements up to 27,500 ng/µL (dsDNA) with extended auto-range path length technology
• No need for cuvettes or consumables needed so you can pipette directly onto pedestal sample retention system
• Pre-programmed applications designed specifically for life scientists
• Enhanced connectivity with data transfer via USB, Ethernet, Bluetooth™ and WiFi options; PC software available for data management

Qubit 4 Fluorometer
• Fast and highly accurate quantitation of DNA, RNA, and protein in less than three seconds per sample
• Measures intact RNA in less than 5 seconds per sample
• High levels of accuracy using only 1–20 μL of sample, even with very dilute samples
• On-board Reagent Calculator quickly generates Qubit working solution preparation instructions
• Stores results from up to 1000 samples
• Large 5.7-inch, state-of-the-art color touchscreen for easy workflow navigation
• Graphical display indicates when samples are in the extended range or out of range
• Small footprint saves space on your bench
• Exports data to a USB drive or directly to your computer via a USB cable
• Ability to personalize your Qubit fluorometer with the assays you run most, add new assays, or even create your own assays with the MyQubit software and web tool
• Language of your choice including English, French, Spanish, Italian, German, simplified Chinese, and Japanese

Qubit™ 1X dsDNA HS Assay Lambda Standard Invitrogen™

The Qubit dsDNA HS (High-Sensitivity) Assay Lambda Standard can be used with either the Qubit 1X dsDNA HS Assay Kit or the Qubit dsDNA HS Assay Kit. Sufficient material is supplied to perform standards with up to 500 samples.

Note: 500-mL thin-walled PCR tubes (Cat. No. Q32856) are required for all Qubit assays and are not included with this Qubit assay standard.

Qubit™ Protein Assay Kit Invitrogen™

The Qubit Protein Assay Kit is designed specifically for use with the Qubit Fluorometer. Using between 1 and 20 µl of your sample, this assay can quantitate samples ranging from 12.5 µg⁄ml to 5 mg⁄ml and exhibits low protein to protein variation. The assay is highly selective for proteins and is designed to be accurate in the presence of reducing reagents, but not in the presence of a large amount of detergent. Common contaminants, such as reducing reagents (DTT, β-mercaptoethanol), salts, free nucleotides, amino acids, solvents, or DNA, but not detergents, are well tolerated in the assay. Slight protocol modifications are required for other contaminants. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted BSA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration.

Which product to choose for fluorometric protein quantitation?
• For 1–20 samples: use this Qubit Protein Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT Protein Assay Kit with microplate reader

Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.

Qubit™ microRNA Assay Kit Invitrogen™

The Qubit microRNA Assay Kit allows easy and accurate quantification of microRNA (miRNA) using the Qubit Fluorometer, even in the presence of common contaminants such as salts, free nucleotides, solvents, detergents, and protein. The assay is highly selective for miRNA over rRNA or large mRNA (>1000 bp). Although the reagent is not exclusively selective for miRNA, we have been able to reproducibly quantify miRNA in pure samples at levels as low as 0.5 ng in the assay tube following the supplied protocol below, even in the presence of mRNA.

Features of Qubit microRNA assay:
• Accurately detects as little as 0.5 ng miRNA in the assay tube
• Core dynamic range of 5–500 ng/mL
• Accurate results for sample concentrations from 50 ng/mL to 100 µg/mL

The Qubit microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using the Qubit Fluorometer. Because the assay tolerates 1–20 µL of sample in an assay volume of 200 µL, accurate results can be obtained for initial sample concentrations from 50 ng/mL–100 µg/mL.

Which product to choose for microRNA quantitation?

• For 1–20 samples: use this Qubit microRNA Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT microRNA Assay Kit with microplate reader
• For >2000 samples: use the Quant-iT microRNA Reagent with microplate reader

Notes:
1. The Qubit microRNA assay file is included on all Qubit 3 and Qubit 4 fluorometers, but may require installation onto the Qubit 2.0 Fluorometer. Uploading new assays to the Qubit 2.0 Fluorometer requires version 3.10 or later of the Qubit firmware.
2. 500 µL thin-walled PCR tubes are required but not included.

Qubit™ dsDNA HS Assay Kit Invitrogen™

The Qubit dsDNA HS (High Sensitivity) Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.

Which product to choose for dsDNA HS (High Sensitivity) quantitation?
• For 1–20 samples: use this Qubit dsDNA HS Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT dsDNA HS Assay Kit with microplate reader

Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.

Qubit™ 4 Fluorometer, with WiFi Invitrogen™

The Qubit 4 Fluorometer with WiFi is the latest version of the popular Qubit fluorometer designed to accurately measure DNA, RNA, and protein quantity, and now also RNA integrity and quality, using the highly sensitive Qubit assays. The Qubit 4 Fluorometer was re-engineered to enable data transfer via WiFi as well as to run the Qubit RNA IQ (integrity & quality) assay. The Qubit 4 Fluorometer and RNA IQ Assay Kit work together to accurately distinguish intact from degraded RNA in just two easy steps.

For all Qubit assays, the concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result. The easy-to-use touch-screen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.

Key features of the Qubit 4 Fluorometer include:
• Fast and highly accurate quantitation of DNA, RNA, and protein in less than three seconds per sample
• Measures intact RNA in less than 5 seconds per sample
• High levels of accuracy using only 1–20 μL of sample, even with very dilute samples
• On-board Reagent Calculator quickly generates Qubit working solution preparation instructions
• Stores results from up to 1000 samples
• Large 5.7-inch, state-of-the-art color touch screen for easy workflow navigation
• Graphical display indicates when samples are in the extended range or out of range
• Small footprint saves space on your bench
• Flexibility to export data to using the included WiFi dongle, a USB drive, or directly to your computer via a USB cable
• Ability to personalize your Qubit fluorometer with the assays you run most, add new assays, or even create your own assays with the MyQubit software and web tool
• Language of your choice including English, French, Spanish, Italian, German, simplified Chinese, and Japanese
• Pre-loaded program to test performance of fluorometer using the Qubit Fluorometer Verification Assay Kit

Learn more about the Qubit Fluorometer ›

The Qubit 4 Fluorometer can also be used to directly measure the fluorescence of samples using Fluorometer mode. Additionally, Ion Sphere Particle quality can be evaluated on the Qubit 4 Fluorometer using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion PGM sequencer.

Enables more accurate results than with UV absorbance at low concentrations
The Qubit 4 Fluorometer is far more sensitive than UV absorbance, which measures anything absorbing at 260 nm, including DNA, RNA, protein, free nucleotides, or excess salts. Moreover, UV spectrophotometry is often not sensitive enough to accurately measure low concentrations of DNA and RNA. With the Qubit fluorometer, your research is enhanced by more accurate measurements because the dyes in the Qubit assay kits fluoresce only when bound to the selected molecule (DNA, RNA or protein) in your sample. This allows you to avoid repeating work due to inaccurate measurements.

Fast, simple, and accurate measurement of RNA quality
The Qubit RNA IQ assay provides a fast, simple method to check whether an RNA sample has degraded. The assay utilizes two unique dyes: one that binds to large, intact RNA, and another that selectively binds to small, degraded RNA. Together they enable you to quickly assess the quality and integrity of an RNA sample. To use, simply add your samples to the RNA IQ working solution, then measure on the Qubit 4 Fluorometer.

Save samples and bench space
The thoughtfully designed Qubit 4 Fluorometer requires samples of only 1–20 µL. It is intended for use at room temperature and occupies only a small amount of bench space. The Qubit 4 Fluorometer features advanced optics and data analysis algorithms, and comes equipped with a WiFi dongle, a USB thumb drive, and cable for data transfer to Excel™ software and for software downloads, as well as a universal power supply, four plug adapters, and electromagnetic CE certification. The Qubit 4 Fluorometer enables maximum flexibility for data export using WiFi, USB drive, or USB cable direct to computer. Additionally, all data can be saved in the Qubit cloud application, including direct transfer from the instrument via WiFi.

Single sample measurements are a breeze
This user-friendly fluorometer is combined with quick and simple assay procedures. Calculations and settings are automatically performed by the instrument. The Qubit assays for use with the Qubit 4 Fluorometer are all performed using the same general protocol, which uses a simple mix-and-read format with incubation times of only two minutes for the DNA and RNA assays. 500 L thin-walled PCR tubes (Cat. No. Q32856) are required.

Qubit™ 4 NGS Starter Kit, with WiFi Invitrogen™

The Qubit 4 NGS Starter Kit for next-generation sequencing includes:
• One Qubit 4 Fluorometer (Cat. No. Q33226)
• Qubit Assay Tubes (set of 500) (Cat. No. Q32856)
• WiFi Dongle (Cat. No. A26774)
• Qubit 1X dsDNA HS Assay Kit, 500 assays

The Qubit 4 Fluorometer with WiFi is the latest version of the popular Qubit fluorometer designed to accurately measure DNA, RNA, and protein quantity, and now also RNA integrity and quality, using the highly sensitive Qubit assays. The Qubit 4 Fluorometer was re-engineered to enable data transfer via WiFi as well as to run the Qubit RNA IQ (integrity & quality) assay. The Qubit 4 Fluorometer and RNA IQ Assay Kit work together to accurately distinguish intact from degraded RNA in just two easy steps.

For all Qubit assays, the concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result. The easy-to-use touch-screen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.

Key features of the Qubit 4 Fluorometer include:
• Fast and highly accurate quantitation of DNA, RNA, and protein in less than three seconds per sample
• Measures intact RNA in less than 5 seconds per sample
• High levels of accuracy using only 1–20 μL of sample, even with very dilute samples
• On-board Reagent Calculator quickly generates Qubit working solution preparation instructions
• Stores results from up to 1000 samples
• Large 5.7-inch, state-of-the-art color touch screen for easy workflow navigation
• Graphical display indicates when samples are in the extended range or out of range
• Small footprint saves space on your bench
• Flexibility to export data to using the included WiFi dongle, a USB drive, or directly to your computer via a USB cable
• Ability to personalize your Qubit fluorometer with the assays you run most, add new assays, or even create your own assays with the MyQubit software and web tool
• Language of your choice including English, French, Spanish, Italian, German, simplified Chinese, and Japanese
• Pre-loaded program to test performance of fluorometer using the Qubit Fluorometer Verification Assay Kit

Learn more about the Qubit Fluorometer ›

The Qubit 4 Fluorometer can also be used to directly measure the fluorescence of samples using Fluorometer mode. Additionally, Ion Sphere Particle quality can be evaluated on the Qubit 4 Fluorometer using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion PGM sequencer.

Enables more accurate results than with UV absorbance at low concentrations
The Qubit 4 Fluorometer is far more sensitive than UV absorbance, which measures anything absorbing at 260 nm, including DNA, RNA, protein, free nucleotides, or excess salts. Moreover, UV spectrophotometry is often not sensitive enough to accurately measure low concentrations of DNA and RNA. With the Qubit fluorometer, your research is enhanced by more accurate measurements because the dyes in the Qubit assay kits fluoresce only when bound to the selected molecule (DNA, RNA or protein) in your sample. This allows you to avoid repeating work due to inaccurate measurements.

Fast, simple, and accurate measurement of RNA quality
The Qubit RNA IQ assay provides a fast, simple method to check whether an RNA sample has degraded. The assay utilizes two unique dyes: one that binds to large, intact RNA, and another that selectively binds to small, degraded RNA. Together they enable you to quickly assess the quality and integrity of an RNA sample. To use, simply add your samples to the RNA IQ working solution, then measure on the Qubit 4 Fluorometer.

Save samples and bench space
The thoughtfully designed Qubit 4 Fluorometer requires samples of only 1–20 µL. It is intended for use at room temperature and occupies only a small amount of bench space. The Qubit 4 Fluorometer features advanced optics and data analysis algorithms, and comes equipped with a WiFi dongle, a USB thumb drive, and cable for data transfer to Excel™ software and for software downloads, as well as a universal power supply, four plug adapters, and electromagnetic CE certification. The Qubit 4 Fluorometer enables maximum flexibility for data export using WiFi, USB drive, or USB cable direct to computer. Additionally, all data can be saved in the Qubit cloud application, including direct transfer from the instrument via WiFi.

Single sample measurements are a breeze
This user-friendly fluorometer is combined with quick and simple assay procedures. Calculations and settings are automatically performed by the instrument. The Qubit assays for use with the Qubit 4 Fluorometer are all performed using the same general protocol, which uses a simple mix-and-read format with incubation times of only two minutes for the DNA and RNA assays. 500 L thin-walled PCR tubes (Cat. No. Q32856) are required.
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