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RapidFinder™ Quant Multi-Meat Set

The RapidFinder™ Quant Multi-Meat Set, when used with one or more of the RapidFinder™ Meat ID kits listed below, enables the determination of the percentage of a target meat species with respect to total meat in food and feed samples. Using the included standards, the kit enables quantification of as little as 0.05% target species DNA as compared to other meat DNA in only a few hours.

The RapidFinder Quant Multi-Meat Set is designed to be used in combination with one or more of the following meat species-specific ID kits (available separately):

NameCatalog Number
RapidFinder™ Beef ID KitA24391
RapidFinder™ Pork ID KitA24392
RapidFinder™ Equine ID KitA15570
RapidFinder™ Poultry ID KitA24397
RapidFinder™ Chicken ID KitA24393
RapidFinder™ Turkey ID KitA24394


• Simple-real-time PCR based assay, no electrophoresis needed
• Flexible-compatible with any food or feed sample, both raw and processed
• Reliable-Includes multi-meat DNA quantification standard
• Fast-from sample to result in the same day

How It Works
The method relies on quantitative real-time PCR. First, a standard curve is created by serially diluting the included multi-meat DNA standard. Then assays for the target species DNA (from one of the kits listed above) and for all meat DNA (from the RapidFinder™ Quant Multi-Meat Set) are run side-by-side on both the food sample DNA and the DNA standards. This approach enables rapid and accurate quantification and reporting of the percentage of target meat species as compared to total meat in a sample. Included in each reaction mix is an internal positive control detected in the VIC® dye channel, which is used to verify the absence of PCR inhibitors.

The RapidFinder™ Quant Multi-Meat Set is compatible with Applied Biosystems® StepOne™, StepOnePlus™, 7500, 7500 Fast, and 7300 real-time PCR systems.

Sample Preparation
DNA sample preparation for the RapidFinder™ meat species identification and quantification workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

RapidFinder™ Quant Equine Set

The RapidFinder™ Quant Equine Set, when used with the RapidFinder™ Equine ID Kit, enables the fast and accurate quantification of horse meat in food and feed samples. Using the included standards, the kit enables quantification of as little as 0.05% horse meat DNA relative to other meat DNA in only a few hours.

• Simple—real-time PCR based assay, no electrophoresis needed
• Flexible—compatible with any food or feed sample, both raw and processed
• Reliable—includes equine DNA quantification standard
• Fast—from sample to result on the same day

How It Works
The kit enables the quantitative real-time PCR-based generation of a standard curve using the included Equus caballus DNA standard. Then assays for both equine DNA and all meat DNA, detected in the FAM™ channel, are run side by side on both food samples and the standards. This approach enables rapid and accurate quantification and reporting of the percentage of horse meat as compared to other meat species in a sample. Included in each reaction mix is an internal positive control detected in the VIC® channel, which is used to verify the absence of PCR inhibitors.

The RapidFinder™ Quant Equine Set is designed to be used with the RapidFinder™ Equine ID Kit, and is compatible with Applied Biosystems® StepOne™, StepOnePlus™, 7500, 7500 Fast, and 7300 Real-time PCR Systems.

Sample Preparation
DNA sample preparation for the RapidFinder™ Equine ID Kit and RapidFinder™ Quant Equine Set workflow is accomplished with the easy-to-use GMO Extraction Kit. Using 10 g of sample rather than smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

RapidFinder™ Equine ID Kit

The RapidFinder™ Equine ID Kit enables the simple and reliable detection of horse meat in food and feed samples. The method works by detection of horse (Equus caballus) DNA by real-time PCR. Unlike ELISA based methods, the RapidFinder™ Equine ID Kit detects both raw and processed horse meat and provides sensitivity down to 0.01% horse meat DNA when used with the GMO Extraction Kit. A positive control is included in the kit (0.1% equine DNA) that can be used as a reference to set the threshold of detection of the assay.

• Simple—real-time PCR based assay, no electrophoresis needed
• Flexible—equine DNA detected in any food or feed sample, both raw and processed
• Reliable—internal positive control (IPC) provides process control check for PCR inhibitors
• Fast—from sample to result on the same day

How the RapidFinder™ Equine Kit Works
Equine DNA detection is accomplished by real-time PCR using the supplied primer/probe set specific for a mitochondrial DNA sequence that is unique to Equus caballus. The target sequence, detected in the FAM™ channel, is not only specific for horse, but also stable. Included in each reaction mix is an internal positive control detected in the VIC® channel, used to verify the absence of PCR inhibitors.

By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control is provided as 0.1% horse DNA in a background of beef DNA, and by comparing sample results to control results, a threshold of detection is provided.

The assay is compatible with Applied Biosystems® StepOne™, StepOnePlus™, 7500, 7500 Fast, and 7300 real-time PCR systems.

Sample Preparation
DNA sample preparation for the RapidFinder™ Equine ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit. Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

Quantification
If a positive result is obtained with the RapidFinder™ Equine ID Kit, you can quantify the amount of equine DNA in the sample using the RapidFinder™ Quant Equine Set.