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    Products

    QuantStudio™ 5 Real-Time PCR System, 96-well, 0.1 mL Applied Biosystems™

    The Applied Biosystems® QuantStudio® 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio® 5 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 6 decoupled channels and white LED) and featuring six independent Veriflex® temperature zones, the QuantStudio® 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio® 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.

    Quantstudio 3/5 Firmware and Design & Analysis Software >

    Features of the QuantStudio® 5 system with 96-well block include:

    • Proven OptiFlex® technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
    • User account management, locked workflow, and Pause features for greater control of experiment data
    • Built-in software features to support compliance with 21 CFR Part 11 guidelines
    • Secure cloud-based software provides increased accessibility and storage options
    • Simplified instrument software with interactive touchscreen for greater ease of use
    • 96-well 0.2mL format or 96-well 0.1mL format

    Thermo Fisher Cloud
    Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.

    Easy to use
    The QuantStudio® 5 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

    Design and analysis software options:

    Option 1: QuantStudio® Design and Analysis cloud software
    • Supports web browser-based system configuration with PC or MAC computer
    • Streamlined software for improved usability and response time
    • Enables secure access of your data when and where you want through the cloud
    • No software to install, no licenses and no versions to update

    Option 2: QuantStudio® Design and Analysis desktop software
    • Supports traditional co-located computer system configuration on Windows® 7 OS
    • Streamlined software for improved usability and response time
    • No licenses to purchase

    Total control
    With built-in software features to support compliance with 21 CFR Part 11 guidelines, user account management, locked workflow and Pause features, the QuantStudio® 5 system offers users greater control of experiment data

    Multiplex performance results that you can trust
    Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex® technology. In the 96-well system this technology features 6 decoupled excitation and emission filter channels, for a total of 21 filter combinations, with white LED.

    Sensitive data analysis
    Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

    High application versatility
    Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

    QuantStudio™ 3 Real-Time PCR System, 96-well, 0.2 mL, laptop Applied Biosystems™

    The Applied Biosystems® QuantStudio® 3 Real-Time PCR System is designed for users who need an affordable, easy-to-use real-time PCR system that doesn't compromise performance and quality. The simplified Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio® 3 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 4 coupled channels and white LED) and featuring three independent Veriflex® temperature zones, the QuantStudio® 3 system enables improved data accuracy and sensitivity for a broad range of genomic applications.

    Quantstudio 3/5 Firmware and Design & Analysis Software >

    Features of the QuantStudio® 3 system include:

    • Simplified instrument software with interactive touchscreen for greater ease of use
    • Proven OptiFlex® technology (4 coupled channels with white LED) for trusted results
    • Secure cloud-based software option provides increased accessibility and storage options
    • User account management, locked workflow, and Pause features for greater control of experiments
    • 96-well 0.2 mL format or 96-well 0.1 mL format

    Thermo Fisher Cloud
    Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.

    Easy to use
    The QuantStudio® 3 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

    Design and analysis software options:

    Option 1: QuantStudio® Design and Analysis cloud software
    • Supports web browser-based system configuration with PC or MAC computer
    • Streamlined software for improved usability and response time
    • Enables secure access of your data when and where you want through the cloud
    • No software to install, no licenses and no versions to update

    Option 2: QuantStudio® Design and Analysis desktop software
    • Supports traditional co-located computer system configuration on Windows® 7 OS
    • Streamlined software for improved usability and response time
    • No licenses to purchase

    Results you can trust
    Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex® technology, which features 4 coupled excitation and emission filter channels with white LED.

    Sensitive data analysis
    Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

    High application versatility
    Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

    QuantStudio™ 7 Flex Real-Time PCR System, 96-well Fast, laptop Applied Biosystems™

    The Applied Biosystems® QuantStudio™ 7 Flex Real-Time PCR System advances your research further by enabling a broad range of real-time PCR-based applications through its multiplexing capabilities and interchangeable block formats. With a simplified workflow, intuitive software, automation capabilities, and touch-screen interface, the QuantStudio™ 7 Flex system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation.

    Features of the QuantStudio™ 7 Flex Real-Time PCR System include:

    • Increased application versatility through the accomodation of 4 different block types
    • Compatible with over 8 million TaqMan® assays for gene expression, genetic variation, gene regulation, or protein expression experiments
    • The intelligent design of the QuantStudio™ platform combined with Applied Biosystems® instrument reliability and accuracy
    • Easy-to-use software, responsive touch-screen, automation capabilities, and effortless block exchange without the need for any tools

    Thermo Fisher Cloud
    Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

    >> Download a 90-day trial of the QuantStudio™ 7 software

    The QuantStudio™ 7 Flex system accommodates the interchange of a 96-well, 96-well Fast, 384-well, or TaqMan® array card block. The system is delivered with one block type (a 96-well Fast block in this case); other block types may be purchased separately. In addition, a Twister® Robot Automation Accessory is available for this system to support high throughput workflows. For a more value-priced system that does not support TaqMan® array cards or automation (but that can be upgraded to do so at a later date), please see our QuantStudio™ 6 Flex system. And for the ultimate in flexibility, consider the QuantStudio™ 12K Flex system, which also accommodates TaqMan® OpenArray® plates.

    Accomplish More, Faster
    Run hundreds of real-time PCR reactions effortlessly using TaqMan® Array 384-well microfluidic cards. And when integrated with the Twister® Robot Automation Accessory (available separately), the QuantStudio™ 7 Flex system maximizes your throughput capabilities.

    High Application Versatility
    Simple workflows, reliable assays and reagents, multiple formats, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, High Resolution Melt (HRM), and Protein Thermal Shift™ experiments.

    Results You Can Trust
    Improved well-to-well and instrument-to-instrument data accuracy is achieved with the OptiFlex™ system, which features 6 decoupled excitation and emission filter channels with 21 filter combinations for maximum multiplexing and chemistry flexibility.

    Sensitive Data Analysis
    Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

    QuantStudio™ 3 Real-Time PCR System, 96-well, 0.1 mL Applied Biosystems™

    The Applied Biosystems® QuantStudio® 3 Real-Time PCR System is designed for users who need an affordable, easy-to-use real-time PCR system that doesn't compromise performance and quality. The simplified Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio® 3 system provides access to your data wherever and whenever you want. Using proven OptiFlex® technology (featuring 4 coupled channels and white LED) and featuring three independent Veriflex® temperature zones, the QuantStudio® 3 system enables improved data accuracy and sensitivity for a broad range of genomic applications.

    Quantstudio 3/5 Firmware and Design & Analysis Software >

    Features of the QuantStudio® 3 system include:

    • Simplified instrument software with interactive touchscreen for greater ease of use
    • Proven OptiFlex® technology (4 coupled channels with white LED) for trusted results
    • Secure cloud-based software option provides increased accessibility and storage options
    • User account management, locked workflow, and Pause features for greater control of experiments
    • 96-well 0.2 mL format or 96-well 0.1 mL format

    Thermo Fisher Cloud
    Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use.

    Easy to use
    The QuantStudio® 3 Real-Time PCR System is compact in form, factory calibrated and is delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

    Design and analysis software options:

    Option 1: QuantStudio® Design and Analysis cloud software
    • Supports web browser-based system configuration with PC or MAC computer
    • Streamlined software for improved usability and response time
    • Enables secure access of your data when and where you want through the cloud
    • No software to install, no licenses and no versions to update

    Option 2: QuantStudio® Design and Analysis desktop software
    • Supports traditional co-located computer system configuration on Windows® 7 OS
    • Streamlined software for improved usability and response time
    • No licenses to purchase

    Results you can trust
    Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex® technology, which features 4 coupled excitation and emission filter channels with white LED.

    Sensitive data analysis
    Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

    High application versatility
    Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

    PowerUp™ SYBR™ Green Master Mix Applied Biosystems™

    PowerUp™ SYBR® Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp™ SYBR® Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.

    PowerUp™ SYBR® Green Master Mix features include:
    • A dual hot-start mechanism for excellent specificity
    • Highly reproducible CTs over a broad dynamic range
    • Inclusion of UDG to help prevent carryover contamination
    • Stability of pre-assembled reactions for up to 72 hours
    • Compatibility with most real-time qPCR instruments

    Exceptional specificity with dual hot-start mechanism
    Specificity is paramount in obtaining high-quality data in SYBR® Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding. PowerUp™ SYBR® Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. In an experiment evaluating 24 different primer sets, PowerUp™ SYBR® Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification.

    Tight reproducibility in CTs over a wide dynamic range
    PowerUp™ SYBR® Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested (see figure). In addition, this master mix provides efficient amplification over 6 logs of sample input. Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR® Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality (see figure).

    UDG included for carryover contamination control
    The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR® Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives.

    Stability of pre-assembled reactions
    The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR® Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data (see figure). This consistency allows users to have confidence and flexibility in the use of PowerUP™ SYBR® Green Master Mix across numerous workflow scenarios1.

    Broad instrument compatibility
    PowerUp™ SYBR® Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments2. It is also compatible with the Bio-Rad IQ™5 and CFX96™ / CFX384™ systems, Roche LightCycler® LC480, and Stratagene® MX3005P® systems. For optimal results, recommended primer concentrations are 300–800 nM.

    Learn more about PowerUp™ SYBR® Green Master Mix >

    1 Pre-PCR stability is influenced not only by the master mix, but also by the target being analyzed. For maximum confidence, researchers should confirm stability profiles of their specific targets.
    2 For optimal performance, standard cycling using the Applied Biosystems® 7900 system is recommended.

    TaqMan™ Fast Advanced Master Mix Applied Biosystems™

    TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms. It provides best in class performance, including the widest quantifiable range, in both single and duplex reactions, even with challenging targets. TaqMan Fast Advanced Master Mix contains AmpliTaq Fast DNA Polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components. It is supplied at a 2X concentration.

    Features of the TaqMan Fast Advanced Master Mix include:
    Best-in-class performance—superior sensitivity, accuracy, dynamic range, and specificity compared to standard mixes in standard mode
    Engineered for enhanced benchtop stability—stable at room temperature for up to 72 hours in preassembled reactions
    Optimized for multiplexing—validated for duplexing with exogenous and endogenous internal positive control assays
    Reduced run times (<40 minutes) on fast and standard instrumentation—optimized for fast mode on fast instruments and fast cycling conditions on standard instruments
    Seamlessly transitions into your experiments—validated with TaqMan assays for gene expression and microRNAs, and TaqMan array micro-fluidic cards

    Best-in-class performance
    TaqMan Fast Advanced Master Mix has been designed to provide performance equal to or better than standard master mixes. It has been benchmarked against the leading suppliers' standard and fast master mixes to help ensure that it succeeds in providing best-in-class sensitivity, accuracy, dynamic range, and specificity. TaqMan Fast Advanced Master Mix’s dynamic range (up to 7 logs) is the widest in the industry. The impressive sensitivity of the mix is showcased in the figure below which compares the Ct values of TaqMan Fast Advanced Master Mix and TaqMan Universal PCR Master Mix across a panel of gene expression assays.

    Benchtop stability for high-throughput handling and convenience
    TaqMan Fast Advanced Master Mix has been engineered to retain a high level of performance in preassembled reactions for up to 72 hours. The stability of this mix provides users of high-throughput liquid handling systems the assurance that the results on the first plate will mimic those of the last plate. The figure below shows an assay that was run upon assembly (time 0) and after 72 hours of incubation at 30°C, simulating the most extreme room temperature scenario.

    For those with less extreme throughput needs, the enhanced stability of this master mix provides an overall added convenience to your workflow, as you are no longer constrained to immediately running plates upon assembly.

    Optimized for multiplexing
    We realize that confidence is paramount when it comes to your results. For added confidence in the results in every well, TaqMan Fast Advanced Master Mix has been designed to help deliver accurate results for duplex reactions using an internal positive control (IPC). The figure below shows results for the experimental target β-actin (gene name: ACTB), which was serially diluted and amplified in single-target reactions and duplex reactions.

    Validated for microRNA assays
    TaqMan Fast Advanced Master Mix has been validated for use with multiple real-time PCR applications, including microRNA assays. The formulation provides high specificity and dynamic range, the two most critical performance attributes that define successful results when working with microRNAs. The data in the figure below demonstrate excellent PCR linearity over a 6-log range of input template.

    Reduced run times on standard instrumentation
    TaqMan Fast Advanced Master Mix has been optimized for use with both fast and standard instrumentation, enabling researchers who currently own standard instruments to also reap the performance benefits and time savings this mix provides. The figure below showcases the impressive results achieved when using TaqMan Fast Advanced Master Mix under fast thermal cycling conditions on the Applied Biosystems 7300 Real-Time PCR System. The mix has been tested with all Applied Biosystems standard real-time PCR instrumentation (7900HT, 7500, and 7300 systems) to enable success independent of whether or not you own a fast-enabled instrument.

    TaqMan™ Fast Virus 1-Step Master Mix Applied Biosystems™

    TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR even in the presence of challenging PCR inhibitors. The 4X formulation provides enhanced detection of both RNA and DNA viruses and is ideal for multiplex gene expression studies, even with low target input. The TaqMan Fast Virus 1-Step Master Mix provides a single-tube format for uniform handling and processing, while delivering qPCR cycling in <30 minutes, helpful when working in high throughput workflows.

    Key product features:
    High sensitivity—4X master mix to amplify both RNA and DNA with improved sensitivity
    Consistent results—formulated to handle common RT-PCR inhibitors found in blood, stool, and other difficult samples
    Simplified application— one-tube, one-step master mix, offering a single run profile with RNA and DNA, which allows for easy mix-and-match of targets on a plate
    Target flexibility—works in singleplex, multiplex, and with exogenous or endogenous internal controls
    High efficiency—increased qRT-PCR speed on fast and on standard instruments

    High sensitivity
    Many common research samples with viral nucleic acids have very low levels of target. We have optimized the master mix for high-sensitivity detection of viral targets. The higher-concentration master mix allows you to set up smaller reactions, which means that you can perform fast cycling protocols and obtain at least the same sensitivity as is expected from standard-cycling qPCR. Alternatively, larger sample input amounts can be added to standard reaction volumes for more accurate quantification of low-titer samples (Figure 1).

    Consistent results in the presence of inhibitors
    Research samples commonly assayed for viruses include blood, dirt, and tissues. Buffer components and proprietary additives in the TaqMan Fast Virus 1-Step Master Mix have been optimized to handle RT-PCR inhibitors (Figure 2) to help ensure consistent performance even with these difficult samples, so that you can be more confident in your results.

    Flexibility in targets
    It is common for labs to test for both RNA and DNA viruses in a variety of samples. To simplify your experiments, a single TaqMan Fast Virus 1-Step Master Mix protocol has been developed to assay both types of nucleic acid, so you can perform RNA and DNA virus queries next to each other on the same plate using the same handling steps (Figure 3). Additionally, because virus research often includes multiplexed primers and probes and internal reaction controls, we have optimized the master mix to work with multiple target amplicons (Figure 4).

    Fast results
    The TaqMan Fast Virus 1-Step Master Mix speeds your time to results and maximizes the use of your real-time PCR instruments with a fast protocol. The high-concentration formulation allows for more target nucleic acid sample to be added in the smaller reaction volumes required to run fast protocols. This enables you to maintain sensitivity with low-titer research samples while improving speed and throughput (Figure 5).

    TaqMan Fast Virus 1-Step Master Mix provides a reliable and efficient reagent for real-time RT-PCR of virus samples that does not sacrifice accuracy. Its robust performance in the presence of common RT-PCR inhibitors and its convenient and flexible reaction setup allows you to have more confidence in your results.

    TaqPath™ ProAmp™ Master Mix Applied Biosystems™

    TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples. It is designed to deliver sensitive and confident results from genomic DNA targets on a broad range of qPCR instrument platforms. TaqPath ProAmp Master Mix is manufactured under a strong ISO13485 quality management system and is designed to produce consistent product performance lot after lot.

    Request an evaluation sample ›

    Two TaqPath ProAmp Master Mix formulations for different throughput needs
    • TaqPath ProAmp Master Mix—includes ROX passive reference dye for lower multiplexing of up to three targets
    TaqPath ProAmp Multiplex Master Mix—includes MUSTANG PURPLE passive reference dye for higher multiplexing of up to four targets

    Benefits of TaqPath ProAmp Master Mix
    Exceptional data quality—high specificity, dynamic range*, and reproducibility for genotyping and copy number determination even in the presence of inhibitors
    Tolerance of inhibitors common in samples prepared from human or animal sources (buccal swabs, blood, and card punches)
    72-hour pre-PCR benchtop stability for automated workflows, enabled by our proprietary Dual-Lock Taq DNA hot-start mechanism
    Multiplexing breadth—two formulations that enable single to four-target detection per reaction
    Excellent manufacturing consistency—manufactured in an ISO 13485-certified facility

    High accuracy genotyping with purified or crude lysate sample inputs
    Accurate genotyping is important when limited sample is available or PCR inhibitors are unavoidable, such as in clinical diagnostic testing. TaqPath ProAmp Master Mix has been optimized to yield accurate and reproducible results, even in low-volume reactions prepared with purified or crude lysate sample inputs. The figure below shows a comparison of genotyping accuracy versus competitor mixes for the same set of TaqMan genotyping assays using crude lysate from blood samples. TaqPath ProAmp Master Mix has been optimized to provide high-specificity allelic discrimination as demonstrated by excellent cluster separation in genotyping plots, whether the sample is purified genomic DNA or crude lysate.

    High accuracy copy number quantitation
    The versatile TaqPath ProAmp Master Mix can also be used for highly accurate determination of copy number variation using purified genomic DNA samples. The figure below shows a comparison of copy number variation results using TaqPath ProAmp Master Mix versus two other mixes. TaqPath ProAmp Master Mix enables determination of copy number accurately in samples with up to four copies.

    General purpose reagents
    TaqPath master mixes are general purpose reagents manufactured in an ISO 13485-certified facility and labeled 'For Laboratory Use.'

    Learn more about multiplexing, TaqPath probes, and how our products can help with your projects ›

    * Dynamic range is a property of both the assay and template concentration in the sample, as well as the formulation of the master mix; thus, individual results may vary.

    QuantStudio™ 7 Flex Real-Time PCR System, 96-well Fast, desktop Applied Biosystems™

    The Applied Biosystems® QuantStudio™ 7 Flex Real-Time PCR System advances your research further by enabling a broad range of real-time PCR-based applications through its multiplexing capabilities and interchangeable block formats. With a simplified workflow, intuitive software, automation capabilities, and touch-screen interface, the QuantStudio™ 7 Flex system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation.

    Features of the QuantStudio™ 7 Flex Real-Time PCR System include:

    • Increased application versatility through the accomodation of 4 different block types
    • Compatible with over 8 million TaqMan® assays for gene expression, genetic variation, gene regulation, or protein expression experiments
    • The intelligent design of the QuantStudio™ platform combined with Applied Biosystems® instrument reliability and accuracy
    • Easy-to-use software, responsive touch-screen, automation capabilities, and effortless block exchange without the need for any tools

    Thermo Fisher Cloud
    Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

    >> Download a 90-day trial of the QuantStudio™ 7 software

    The QuantStudio™ 7 Flex system accommodates the interchange of a 96-well, 96-well Fast, 384-well, or TaqMan® array card block. The system is delivered with one block type (a 96-well Fast block in this case); other block types may be purchased separately. In addition, a Twister® Robot Automation Accessory is available for this system to support high throughput workflows. For a more value-priced system that does not support TaqMan® array cards or automation (but that can be upgraded to do so at a later date), please see our QuantStudio™ 6 Flex system. And for the ultimate in flexibility, consider the QuantStudio™ 12K Flex system, which also accommodates TaqMan® OpenArray® plates.

    Accomplish More, Faster
    Run hundreds of real-time PCR reactions effortlessly using TaqMan® Array 384-well microfluidic cards. And when integrated with the Twister® Robot Automation Accessory (available separately), the QuantStudio™ 7 Flex system maximizes your throughput capabilities.

    High Application Versatility
    Simple workflows, reliable assays and reagents, multiple formats, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, High Resolution Melt (HRM), and Protein Thermal Shift™ experiments.

    Results You Can Trust
    Improved well-to-well and instrument-to-instrument data accuracy is achieved with the OptiFlex™ system, which features 6 decoupled excitation and emission filter channels with 21 filter combinations for maximum multiplexing and chemistry flexibility.

    Sensitive Data Analysis
    Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

    QuantStudio™ 6 Flex Real-Time PCR System, 384-well, desktop Applied Biosystems™

    The Applied Biosystems® QuantStudio™ 6 Flex Real-Time PCR System facilitates real-time PCR based applications with an upgradable block selection, allowing you to plan for experiments today and grow with tomorrow’s needs. With a simplified workflow, intuitive software, and touch-screen interface, the QuantStudio™ 6 Flex system offers exceptional reproducibility at a price you can afford.

    Features of the QuantStudio™ 6 Flex Real-Time PCR System include:

    • Affordable price allows you to obtain a high performance instrument with minimal upfront capital investment
    • Compatible with over 8 million TaqMan® assays for gene expression, genetic variation, gene regulation, or protein expression experiments
    • The intelligent design of the QuantStudio™ platform combined with Applied Biosystems® instrument reliability and accuracy
    • Easy-to-use software, responsive touch-screen, and effortless block exchange without the need for any tools

    Thermo Fisher Cloud
    Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

    >> Download a 90-day trial of the QuantStudio™ 6 software

    The QuantStudio™ 6 Flex system accommodates the interchange of a 96-well, 96-well Fast, or 384-well block. The system is delivered with one block type (a 384-well block in this case); other block types may be purchased separately. For a system that also supports TaqMan® array cards, as well as automation, please see our QuantStudio™ 7 Flex system. The QuantStudio™ 6 Flex system can also be upgraded to a QuantStudio™ 7 Flex system at a later date, as your needs change. For the ultimate in productivity, consider the QuantStudio™ 12K Flex system, which also accommodates TaqMan® OpenArray® plates.

    Upgrade Capabilities When You Need Them

    The QuantStudio™ 6 Flex system can be seamlessly upgraded to a QuantStudio™ 7 Flex system by a field service engineer to give you additional automation, throughput, and multiplexing capabilities.

    Results You Can Trust
    Improved well-to-well and instrument-to-instrument data accuracy is achieved with the OptiFlex® system, which features 5 coupled excitation and emission filter channels.

    Sensitive Data Analysis
    Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

    QuantStudio™ 7 Flex Real-Time PCR System, 384-well, laptop Applied Biosystems™

    The Applied Biosystems® QuantStudio™ 7 Flex Real-Time PCR System advances your research further by enabling a broad range of real-time PCR-based applications through its multiplexing capabilities and interchangeable block formats. With a simplified workflow, intuitive software, automation capabilities, and touch-screen interface, the QuantStudio™ 7 Flex system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation.

    Features of the QuantStudio™ 7 Flex Real-Time PCR System include:

    • Increased application versatility through the accomodation of 4 different block types
    • Compatible with over 8 million TaqMan® assays for gene expression, genetic variation, gene regulation, or protein expression experiments
    • The intelligent design of the QuantStudio™ platform combined with Applied Biosystems® instrument reliability and accuracy
    • Easy-to-use software, responsive touch-screen, automation capabilities, and effortless block exchange without the need for any tools

    Thermo Fisher Cloud
    Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

    >> Download a 90-day trial of the QuantStudio™ 7 software

    The QuantStudio™ 7 Flex system accommodates the interchange of a 96-well, 96-well Fast, 384-well, or TaqMan® array card block. The system is delivered with one block type (a 384-well block in this case); other block types may be purchased separately. In addition, a Twister® Robot Automation Accessory is available for this system to support high throughput workflows. For a more value-priced system that does not support TaqMan® array cards or automation (but that can be upgraded to do so at a later date), please see our QuantStudio™ 6 Flex system. And for the ultimate in flexibility, consider the QuantStudio™ 12K Flex system, which also accommodates TaqMan® OpenArray® plates.

    Accomplish More, Faster
    Run hundreds of real-time PCR reactions effortlessly using TaqMan® Array 384-well microfluidic cards. And when integrated with the Twister® Robot Automation Accessory (available separately), the QuantStudio™ 7 Flex system maximizes your throughput capabilities.

    High Application Versatility
    Simple workflows, reliable assays and reagents, multiple formats, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, High Resolution Melt (HRM), and Protein Thermal Shift™ experiments.

    Results You Can Trust
    Improved well-to-well and instrument-to-instrument data accuracy is achieved with the OptiFlex™ system, which features 6 decoupled excitation and emission filter channels with 21 filter combinations for maximum multiplexing and chemistry flexibility.

    Sensitive Data Analysis
    Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

    QuantStudio™ 7 Flex Real-Time PCR System, array card, desktop Applied Biosystems™

    The Applied Biosystems® QuantStudio™ 7 Flex Real-Time PCR System advances your research further by enabling a broad range of real-time PCR-based applications through its multiplexing capabilities and interchangeable block formats. With a simplified workflow, intuitive software, automation capabilities, and touch-screen interface, the QuantStudio™ 7 Flex system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation.

    Features of the QuantStudio™ 7 Flex Real-Time PCR System include:

    • Increased application versatility through the accomodation of 4 different block types
    • Compatible with over 8 million TaqMan® assays for gene expression, genetic variation, gene regulation, or protein expression experiments
    • The intelligent design of the QuantStudio™ platform combined with Applied Biosystems® instrument reliability and accuracy
    • Easy-to-use software, responsive touch-screen, automation capabilities, and effortless block exchange without the need for any tools

    Thermo Fisher Cloud
    Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

    >> Download a 90-day trial of the QuantStudio™ 7 software

    The QuantStudio™ 7 Flex system accommodates the interchange of a 96-well, 96-well Fast, 384-well, or TaqMan® array card block. The system is delivered with one block type (an array card block in this case); other block types may be purchased separately. In addition, a Twister® Robot Automation Accessory is available for this system to support high throughput workflows. For a more value-priced system that does not support TaqMan® array cards or automation (but that can be upgraded to do so at a later date), please see our QuantStudio™ 6 Flex system. And for the ultimate in flexibility, consider the QuantStudio™ 12K Flex system, which also accommodates TaqMan® OpenArray® plates.

    Accomplish More, Faster
    Run hundreds of real-time PCR reactions effortlessly using TaqMan® Array 384-well microfluidic cards. And when integrated with the Twister® Robot Automation Accessory (available separately), the QuantStudio™ 7 Flex system maximizes your throughput capabilities.

    High Application Versatility
    Simple workflows, reliable assays and reagents, multiple formats, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, High Resolution Melt (HRM), and Protein Thermal Shift™ experiments.

    Results You Can Trust
    Improved well-to-well and instrument-to-instrument data accuracy is achieved with the OptiFlex™ system, which features 6 decoupled excitation and emission filter channels with 21 filter combinations for maximum multiplexing and chemistry flexibility.

    Sensitive Data Analysis
    Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

    QuantStudio™ 12K Flex Real-Time PCR System, OpenArray™ block, Accufill™ System Applied Biosystems™

    The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems real-time technology in a single instrument. The QuantStudio™ 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray® plates, TaqMan® array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it’s now or in the future, for maximum flexibility and consistency in your research. Note: this particular system includes an OpenArray block and AccuFill™ sample handling system. For a package that does not include an AccuFill system, please see Cat. No. 4472380.

    Thermo Fisher Cloud
    Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

    The QuantStudio™ 12K Flex System’s intuitive software, easy touch screen setup, automation integration, effortless block exchange, and easy-to- use OpenArray® components are designed to let you get started right away and reduce hands on time. Plan for the future and save time, money, and space in your lab with the only all-in-one qPCR instrument enabling a broad range of applications with maximum throughput.

    Flexibility & Scalability
    Includes five interchangeable blocks: The QuantStudio™ 12K Flex blocks accomodate 96-well, FAST 96-well, 384-well, TaqMan® array cards, and OpenArray® plates, for effortless scaling from 1 to 12,000 data points in a single 100 minute run. Increase throughput as you dive deeper into your research, using just one instrument. As you begin your project, start with 96-well format experiments and then scale up to a higher throughput to get the statistically significant answers you need. Additionally, you can easily validate and screen your targets of interest with a selected panel of genes using novel formats such as the TaqMan® array cards and OpenArray® plates.
    Compatible with a full range of TaqMan® assays: The QuantStudio™ 12K Flex System is optimized to enable clear, clean data for all TaqMan® assays that might be used across a broad range of applications, including gene expression, microRNA and small noncoding RNAs, SNP genotyping, protein analysis, protein thermal shift, high resolution melt, copy number variation, and pathogen detection.
    Provides a seamless switch from qPCR to digital PCR: Increase the precision and sensitivity of your nucleic acid experiment by switching from qPCR to digital PCR mode using QuantStudio® Digital PCR kits and DigitalSuite Software with the QuantStudio™ OpenArray ® block.
    Delivers peace of mind: Leave early on a Friday and monitor runs from home, knowing that the system can safely store over 100 runs.

    Horsepower
    Enhanced OptiFlex™ System: Building upon Applied Biosystems’ proprietary technology, the Enhanced OptiFlex™ System provides enhanced fluorescence detection for the accurate and sensitive data analysis necessary to collect 12,000 data points in a single 100 minute run.
    Unprecedented coverage and sample throughput: The OpenArray® format accelerates genomic validation and screening, driving over 150,000 data points per work day, enabling most projects to be completed in days instead of weeks.
    Simple OpenArray® workflow: QuantStudio™ Open Array® plates arrive in a case to facilitate ease of handling and ensure optimal data quality upon loading of samples using the Accufill™ System. Easily load four arrays into the QuantStudio™ 12K Flex instrument to achieve over 12,000 data points. Integrated sample tracking software lets you easily track your samples throughout the course of your project.
    Tools to maximize your resources: New intuitive touch-screen interface and integrated calendar feature allows for scheduling time of use on the instrument. Additional analysis software such as DigitalSuite v1.0, ExpressionSuite v1.0, and Genotyper v1.1 is available to help you analyze hundreds of targets and samples and achieve the statistical power you need.

    QuantStudio™ 12K Flex Software
    • Intuitive interface and innovative design for novice and experienced users
    • Convenient walk-away operation
    • Improved data analysis across block types
    • Fully compatible with high-throughput environments

    Expandable Software Architecture
    Optional add-ons are available to upgrade the existing software:
    21 CFR Part 11 compliance: This optional SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability.
    High Resolution Melt (HRM): Using the built-in MeltDoctor™ protocols and calibrations, you can spend less time optimizing the run and more time customizing your results. Define the number of variants you want to see by quickly changing analysis settings.
    DigitalSuite Software v1.0: For OpenArray™ block users needing sensitivity for rare targets and precision to measure small differences in target abundance.
    ExpressionSuite software v1.0: our latest stand-alone gene expression software with enhanced analysis tools that can analyze over 200 384-well plates or 10 OpenArray® plates.

    For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

    QuantStudio™ 6 Flex Real-Time PCR System, 96-well Fast, desktop Applied Biosystems™

    The Applied Biosystems® QuantStudio™ 6 Flex Real-Time PCR System facilitates real-time PCR based applications with an upgradable block selection, allowing you to plan for experiments today and grow with tomorrow’s needs. With a simplified workflow, intuitive software, and touch-screen interface, the QuantStudio™ 6 Flex system offers exceptional reproducibility at a price you can afford.

    Features of the QuantStudio™ 6 Flex Real-Time PCR System include:

    • Affordable price allows you to obtain a high performance instrument with minimal upfront capital investment
    • Compatible with over 8 million TaqMan® assays for gene expression, genetic variation, gene regulation, or protein expression experiments
    • The intelligent design of the QuantStudio™ platform combined with Applied Biosystems® instrument reliability and accuracy
    • Easy-to-use software, responsive touch-screen, and effortless block exchange without the need for any tools

    Thermo Fisher Cloud
    Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

    >> Download a 90-day trial of the QuantStudio™ 6 software

    The QuantStudio™ 6 Flex system accommodates the interchange of a 96-well, 96-well Fast, or 384-well block. The system is delivered with one block type (a 96-well Fast block in this case); other block types may be purchased separately. For a system that also supports TaqMan® array cards, as well as automation, please see our QuantStudio™ 7 Flex system. The QuantStudio™ 6 Flex system can also be upgraded to a QuantStudio™ 7 Flex system at a later date, as your needs change. For the ultimate in productivity, consider the QuantStudio™ 12K Flex system, which also accommodates TaqMan® OpenArray® plates.

    Upgrade Capabilities When You Need Them

    The QuantStudio™ 6 Flex system can be seamlessly upgraded to a QuantStudio™ 7 Flex system by a field service engineer to give you additional automation, throughput, and multiplexing capabilities.

    Results You Can Trust
    Improved well-to-well and instrument-to-instrument data accuracy is achieved with the OptiFlex® system, which features 5 coupled excitation and emission filter channels.

    Sensitive Data Analysis
    Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

    QuantStudio™ Dx Real-Time PCR Instrument with 96-well fast block Applied Biosystems™

    The QuantStudio™ Dx Real-Time PCR Instrument is a real-time PCR platform that delivers the proven performance, flexibility, expandability, and support needed to meet the evolving needs of the clinical laboratory today and tomorrow. The QuantStudio™ Dx instrument is approved for in vitro diagnostic (IVD) use with the Quidel® Molecular Direct C. difficile Assay, the first available from a growing menu of assays that are under development. In Test Development Mode, the QuantStudio™ Dx Real-Time PCR Instrument offers easy-to-change blocks that can also accommodate array cards, as well as 96-well standard, 96-well Fast, and 384-well plates, allowing you to select the format that is right for your throughput needs.

    Built on a legacy of innovation and excellence in real-time PCR, the QuantStudio™ Dx instrument leverages the best of our portfolio of Applied Biosystems® real-time PCR systems, and refines a platform to specifically meet the needs of the diagnostic laboratory.

    Results You Can Trust
    The QuantStudio™ Dx Real-Time PCR Instrument's proven performance, security, and reliability—combined with the outstanding sensitivity and specificity of the Quidel® Molecular Assays—help ensure the right answer is delivered the first time, every time.

    Flexibility You Want, Security You Need
    In Test Development Mode, The QuantStudio™ Dx Real-Time PCR Instrument with the 96-Well fast Block is designed with easily interchangeable thermal cycling blocks to accommodate your laboratory's varying needs for throughput, format type, and assay type. In addition to the 96-well fast format that was cleared for use in the US with the Quidel Molecular Direct C. difficile Assay, the instrument also accepts 96-well standard, 384-well, and array card formats that can be used in Test Development Mode for assay development.

    Efficient Workflows and Assays
    A simplified instrument and assay workflow, as well as intuitive software, make the entire test easy and efficient, from processing to reporting. Plus, it frees up highly trained operators to perform more complex tasks.

    QuantStudio™ Dx Software
    • Track reagent and patient information for quality control purposes
    • Enter reagent information by barcode and upload patient information from Excel files to minimize data entry errors
    • Print calibration and maintenance reports for your instrument records
    • Included SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability as part of 21 CRF Part 11 compliance

    For In Vitro Diagnostic Use. The QuantStudio™ Dx Real-Time PCR Instrument meets the requirements of the in vitro Diagnostic Medical Devices Directive (98/79/EC). The CE IVD-registered QuantStudio™ Dx Real-Time PCR Instrument is for distribution and use in specific countries only.
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