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Products

Protein Expression Sample Prep Kit Applied Biosystems™

The Applied Biosystems® Protein Expression Sample Preparation Kit contains the reagents required for the preparation of target and control cell lysates for use with TaqMan® Protein Expression Assay experiments. The Cell Lysis Reagent is a buffered detergent solution, which is combined with user-supplied protease and phosphatase inhibitors, and used to lyse adherent or nonadherent cultured cells under nondenaturing conditions. The Cell Resuspension Buffer is used to resuspend the cells before adding the lysis reagent and to dilute the lysates in preparation for the assay-binding step.

Si-Carb Sample Prep Kit Thermo Scientific™

The Thermo Scientific™ Si-Carb Sample Prep Kit is useful for preparing intractable samples, such as coatings, paints and hard polymers for diffuse reflectance analysis.

PrepSEQ™ Rapid Spin Sample Prep Kit - Bead Beating Applied Biosystems™

The PrepSEQ® Rapid Spin Sample Prep Kit - Bead Beating was developed for the AFNOR-approved MicroSEQ® Listeria species and Listeria monocytogenes Real-Time PCR detection workflows. A combined spin and bead beating protocol efficiently lyses Listeria from a 750 µL aliquot of enriched food samples (those with little-to-no fat and lean meats) and simple environmental samples (swabs and sponges). The PrepSEQ® Rapid Spin Sample Prep Kit - Bead Beating is designed to meet the needs of lower throughput food laboratories.

NOTE: Difficult samples (e.g., samples containing high fat or otherwise prone to PCR inhibition) should be processed using the PrepSEQ® Rapid Spin Sample Prep Kit - Extra Clean & Bead Beating.

• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput (up to 100 samples a day)
• Inactivates PCR inhibitors and produces high quality DNA for downstream detection
• Uses a simple spin protocol

Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from a broad range of sample types.

Designed for Laboratories that Prepare up to 100 Samples a Day
Spin column-based preparation enables small batch sizes to be managed while requiring only simple benchtop devices to process, thereby limiting the capital expenditure associated with much higher throughput automation devices.

Removes PCR Inhibitors and Produces High Quality DNA
The PrepSEQ® Rapid Spin Sample Prep Kit - Bead Beating offers a simple, cost-effective way to prepare Listeria DNA from enriched cultures. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube, where they are then lysed via a zirconium bead beating step.

TaqMan® E. coli O157:H7 Detection Kit with PrepMan® Ultra Sample Preparation Reagent Applied Biosystems™

The TaqMan® E. coli O157:H7 Detection Kit with PrepMan® Ultra Sample Preparation Reagent combines the simple and reliable target sequence amplification of our TaqMan® pathogen detection kits with the rapid and effective DNA template preparation of our PrepMan® Ultra reagent.

The TaqMan® E. coli O157:H7 Detection Kit utilizes the polymerase chain reaction (PCR) to amplify a target sequence unique to E. coli O157:H7. The kit demonstrates 100% inclusivity for 16 strains of serotypes 0157:H7 and 055:H7. It has also demonstrated 100% exclusivity against 31 other Escherichia coli strains and 21 non-E. coli strains.

The PrepMan® Ultra Sample Preparation Reagent was developed specifically for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step.

For your convenience, both the detection kit and sample preparation reagent are offered in this single product. Sufficient quantities of each are provided for 200 preparations and assays.

For testing of Food and Environmental samples only.

resDNASEQ™ Quantitative HEK293 DNA Kit with PrepSEQ™ Residual DNA Sample Preparation Kit Applied Biosystems™

The resDNASEQ Quantitative HEK293 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host-cell DNA from HEK293 cell lines used in the development of gene therapy, cell-based vaccine, and similar biotherapeutics. Reliable and rapid, the resDNASEQ system enables sensitive and specific quantitation of HEK293 cell DNA*. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples with different sample matrices to purified final product—whether the sample contains high molecular weight or sheared DNA.

This kit is bundled together with sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts. For the quantitation kit alone, please see Cat. No. A46014.

• Highly sensitive quantitation using proven TaqMan real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, TaqMan primer/probe mix, and HEK293 genomic DNA standard

Integrated workflow solution
The resDNASEQ Quantitative HEK293 DNA Kit is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing. The kit is compatible with the PrepSEQ Residual DNA Sample Preparation Kit, which offers both a manual and automated sample preparation workflow. The resDNASEQ kit has been validated on the Applied Biosystems 7500 Fast Real-Time PCR System and the Applied Biosystems QuantStudio 5 Real-Time PCR System. Data analysis is streamlined using AccuSEQ Real-Time PCR Detection Software that provides accurate quantitation and security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance.

Easy to use
With the resDNASEQ Quantitative HEK293 DNA Kit, you perform qPCR analysis to compare the amount of HEK293 DNA in your test samples to a standard curve generated with known amounts of purified HEK293 DNA standard. The resDNASEQ kit includes almost everything you need to carry out these steps, from master mix to TaqMan primer/probe mix to HEK293 genomic DNA standard, as well as step-by-step instructions.

Consistent performance
For accurate quantitation of residual HEK293 DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ system you get consistent assay performance across a range of DNA fragment sizes.

*Due to the high degree of similarity between genomes, DNA from closely-related primate species, such as monkey (Vero), chimpanzee, and orangutan, are expected be detected by the resDNASEQ HEK293 Quantitation Kit.

MicroSEQ™ E. coli O157:H7 Detection Starter Kit, with PrepSEQ™ Rapid Spin Sample Preparation Kit Applied Biosystems™

The MicroSEQ® E. coli O157:H7 Detection Kit detects E. coli O157:H7 simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.

Key product features:
• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software guided 3-step process
• Get your results quickly with time-to-results in as little as 8 hours for a 25g sample
• Genome sequencing using the Applied Biosystems SOLiD™ platform helps ensure high specificity
• Detect 1-3 colony-forming units (CFU) per 25 grams of food
• Get optimal performance on a wide range of food sample and environmental surface types
• Eliminate false negatives with internal positive control

Lyophilized for Efficiency and Ease of Use
For maximum ease of use and consistency of results, the reagents used in the MicroSEQ® E. coli O157:H7 Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Enhanced Specificity
To design an assay highly specific for E. coli O157:H7, the genome of E. coli O55:H7 was sequenced using the next-generation Applied Biosystems® SOLiD™ platform and compared to the publicly available sequence of E. coli O157:H7. Sequences specific to E. coli O157:H7 were then used as targets to design the MicroSEQ® E. coli O157:H7 Detection Kit. The MicroSEQ® E. coli O157:H7 Detection Kit owes its additional specificity to the use of TaqMan® chemistry. Unlike SYBR® Green chemistry that uses only target-specific primers, TaqMan® chemistry uses both primers and probes that are specific to the target to help prevent false positives.

Workflow Flexibility
To better meet the needs of food testing laboratories, the MicroSEQ® E. coli O157:H7 Detection Kit offers the flexibility of three different workflows.

Fast Time to Results
• 8-10 hour time-to-results
• 25 g sample
• BHI enrichment
Standard Media
• 18 hour time to results
• 25 g sample
• BPW enrichment
Larger Volumes
• 18 hour time to results
• 375 g sample of ground beef or beef trim
• BPW enrichment

Simple, Three-Step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps.
1. Enrich sample
2. Prepare DNA
3. Run test

AOAC Certified
The full workflow including the MicroSEQ® E. coli O157:H7 Detection Kit, PrepSEQ™ sample preparation kits, 7500 Fast instrument and RapidFinder™ Express software has earned the Performance Tested MethodsSM certification from the AOAC Research Institute.

Optimized Sample Preparation
The MicroSEQ® E. coli O157:H7 Detection Kit is optimized for use with PrepSEQ™ Sample Preparation Kits. PrepSEQ™ kits reduce pre-enrichment time, enhance assay performance and enable you to enrich and prepare high quality samples from a broad range of sample types for detection of 1-3 CFU per 25 grams of sample.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder™ Express software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

A Complete Solution
The MicroSEQ® E. coli O157:H7 Detection Kit is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make E. coli O15:H7 detection as fast, easy and reliable as possible.

For Research Use Only. Not for use in diagnostics procedures.

EasyPep™ Mini MS Sample Prep Kit Thermo Scientific™

The Thermo Scientific EasyPep Mini MS Sample Prep Kit provides pre-formulated reagents and a robust method for the preparation of high-quality samples for mass spectroscopy (MS) analysis in less than three hours.

Features of the EasyPep Mini MS Sample Prep Kit include:
Complete—includes pre-formulated reagents for lysis through digestion, peptide clean-up columns, and an optimized protocol for up to 20 samples
Optimized—streamlined protocol and reagents minimize the number of steps and time time needed to process samples
Flexible—reagents and protocol have been verified using cells, plasma, and tissue samples for 10 to 100 µg samples
Time-saving—sample processing time reduced from more than one day to less than three hours
Compatible—final preparation is ready for direct MS analysis and other downstream applications, including TMT labeling

The EasyPep Mini MS Sample Prep Kit enables efficient and reproducible processing of cultured mammalian cells and tissues for proteomic MS analysis. The kit contains pre-formulated buffers, MS-grade enzyme mix, peptide clean-up columns, and an optimized protocol to generate MS-compatible peptide samples in less than three hours. The kit is optimized to process protein samples from 10–100 µg with a high yield of MS-ready peptides.

The sample preparation of peptides for MS analysis is complex, with numerous steps and varied protocols, resulting in sample variability in both quality and quantity. This kit has been designed to improve reproducibility while saving hands-on and processing time. The number of steps and processing time have been reduced through the addition of Universal Nuclease to reduce viscosity from nucleic acids (replacing sonication), a rapid 'one pot' reduction/alkylation solution for cysteine modification (carbamidomethylation, + 57.02), and a trypsin/Lys-C protease mix for more complete digestion. In addition, the kit includes Peptide Clean-up columns and buffers to prepare detergent-free peptide samples for MS analysis. The optimized reagents and protocol produce high-quality peptides that are compatible with TMT labeling and other downstream applications, or are ready for MS analysis.

Related products
Pierce Colorimetric Peptide Quantitation Assay
Pierce Trypsin/LysC Protease Mix, MS-Grade
TMT10plex Label Reagent Set

Pierce™ SDS-PAGE Sample Prep Kit Thermo Scientific™

The Thermo Scientific Pierce SDS-PAGE Sample Prep Kit quickly and easily removes high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis of proteins.

Features of the SDS-PAGE Sample Prep Kit:

Eliminates gel artifacts caused by incompatible contaminants—removes dyes, reducing agents, detergents, sugars, glycerol, guanidine, urea and ammonium sulfate to provide reproducible results by SDS-PAGE analysis
Compatible with the BCA protein assay—allows protein quantitation of the processed sample before gel-loading
Enriches dilute protein solutions—concentrates protein samples for SDS-PAGE analysis by six-fold in less than 20 minutes
Powerful and flexible—process 2 to 350 µL of sample containing up to 70 µg of protein; recover 75 to 85% of the total protein in 50 µL of elution buffer
Convenient, complete kit—includes PAGEprep Resin, DMSO, spin cups and sample loading buffer

The SDS-PAGE Sample Prep Kit provides a simple and effective method for concentrating samples while removing chemicals that interfere with SDS-PAGE analysis, such as acids and bases, detergents, guanidine and ammonium sulfate. The proprietary PAGE-prep Resin binds protein in the presence of dimethylsulfoxide (DMSO). Interfering contaminants are then removed by washing the protein-bound resin in an easy-to-use spin cup format. Proteins are eluted in a buffer compatible with the Pierce BCA Protein Assay, allowing quantitation of a portion of the processed sample. The eluted proteins can then be combined with sample buffer and analyzed on the SDS-PAGE system of choice.

Includes:
• PAGEprep resin slurry, DMSO solution, elution buffer, sample buffer, spin cups and collection tubes

Applications:
• Inclusion bodies solubilized in guanidine·HCl
• Samples containing low-pH buffers, thiocyanates or urea
• Proteins precipitated in ammonium sulfate
• Concentration of dilute protein solutions

The SDS-PAGE Sample Prep Kit uses a unique resin composed of modified diatomaceous earth that binds protein in the presence of DMSO. The simple protocol involves combining 2 to 350 µL of sample, containing up to 70 µg of protein, with 20 µL of the PAGEprep Resin in DMSO (supplied). Proteins in the sample bind to the resin in 3 to 5 minutes, after which the nonbound contaminating chemicals are washed away. Finally, the purified proteins are recovered from the resin in 50 µL of Elution Buffer. The recovered protein sample is then ready to mix with the supplied 5X Sample Loading Buffer for gel loading (or another sample loading buffer). The procedure also allows for dilute protein samples to be concentrated up to six-fold, enabling more protein to be loaded per well. In addition, the Thermo Scientific Pierce BCA Protein Assay (Part No. 23225) is compatible with the Elution Buffer and may be used to determine final protein concentration before gel loading.

For reliable SDS-PAGE analysis, protein samples must be prepared in a buffer free of interfering substances and at protein concentrations adequate for analysis. Many interfering substances, which interfere with typical sample buffers for polyacrylamide gel electrophoresis (SDS-PAGE), are difficult to remove by traditional sample preparation methods. For example, protein samples containing 6M guanidine-HCl will precipitate when mixed with Laemmli buffer for SDS-PAGE, causing the sample to run poorly on a gel. Fortunately, samples containing a wide range of interfering chemicals, such as chaotropic agents, detergents, lipids, pH extremes and salts, can be cleaned up within minutes using the SDS-PAGE Sample Prep Kit.

Related Products
Pierce™ SDS-PAGE Sample Prep Kit

NativePAGE™ Sample Prep Kit Invitrogen™

The NativePAGE™ Sample Prep Kit includes sample preparation reagents for native gel electrophoresis. The kit includes two ready-to-use detergent solutions, 10% DDM (n-dodecyl-β-D-maltoside) and 5% Digitonin, which improve the solubility of hydrophobic and membrane proteins during sample preparation.

The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis, showing increased resolution and reduced streaking.

PrepSEQ™ Rapid Spin Sample Preparation Kit - Extra Clean Applied Biosystems™

The PrepSEQ™ Rapid Spin Sample Preparation Kit – Extra Clean is recommended for foods with a high lipid content, such as infant formula and chicken wing samples. The kit was developed for the rapid preparation of DNA from food and environmental samples for use in PCR amplification reactions. Using a simple spin protocol, PrepSEQ™ Rapid Spin efficiently inactivates PCR inhibitors. For laboratories involved in food and environmental testing, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. This has created the demand for a sample preparation method that is fast, cost-effective and capable of extracting high-quality DNA from a broad range of sample types. While such methods have been developed for dedicated, high-throughput laboratories, there is a growing need for methods suitable to low-to-medium throughput applications. The PrepSEQ™ Rapid Spin Kit – Extra Clean meets this need.

• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput work loads (up to 100 samples a day)
• Inactivates PCR inhibitors so you will most likely not have to repeat the template preparation step
• Uses a simple spin protocol
Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from a broad range of sample types including chocolate, soft cheeses, and soil.

Designed for Laboratories that Prepare up to 100 Samples a Day
Kits exist for dedicated, high-throughput laboratories, but now you have a kit that is suitable for low-to-medium throughput applications. This kit is designed for laboratories that routinely prepare up to 100 samples a day.

Removes PCR Inhibitors
The PrepSEQ™ Rapid Spin Sample Preparation Kit offers a simple, cost-effective way to prepare bacterial DNA from broth cultures. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube where it can be stored or used for PCR.

For Research Use Only. Not for use in diagnostics procedures.

resDNASEQ™ Quantitative CHO DNA Kit with PrepSEQ™ Residual DNA Sample Preparation Kit Applied Biosystems™

This resDNASEQ® Quantitative CHO DNA kit is a quantitative PCR (qPCR)-based kit—part of the resDNASEQ® Quantitative CHO DNA System—enables you to detect sub-picogram amounts of residual DNA from the Chinese hamster ovary (CHO) cell line in under 5 hours. The system overcomes the limitations of traditional methods by combining high-recovery PrepSEQ™ sample preparation and TaqMan®-based quantitation of residual DNA.

MycoSEQ™ Mycoplasma Detection and PrepSEQ™ Express Sample Preparation Cartridge Combo Kit Applied Biosystems™

This product consists of one MycoSEQ Mycoplasma Detection Kit and one PrepSEQ Express Sample Preparation Cartridge Kit.

MycoSEQ Mycoplasma Detection Kit
The MycoSEQ Mycoplasma Detection Kit is a real-time PCR assay validated to meet the requirements of European Pharmacopeia Chapter 2.6.7. The primers were designed using a proprietary bioinformatics pipeline to detect >90 mycoplasma species with high specificity and no cross-reactivity to closely-related bacterial species. The assay's sensitivity has been demonstrated by internal and external validations to detect 10 cfu/mL or the genomic equivalent of 10 GC/mL as recommended by regulatory guidance.

Learn more ›

PrepSEQ Express Sample Preparation Cartridge Kit
The PrepSEQ Express Sample Preparation Cartridge Kit uses the established PrepSEQ nucleic acid extraction chemistry for quantitative recovery of DNA and RNA in an optimized cartridge format to be used on the Automate Express magnetic particle processor instrument. The AutoMate Express is an easy-to-use benchtop instrument that enables hands-free automation of the nucleic acid extraction process for mycoplasma contaminant testing.

The AutoMate Express system automatically performs all steps for efficient nucleic acid extraction from contaminants in a closed environment. The system uses PrepSEQ Express kits that contain sealed cartridges with extraction reagents and plastics, including tips and sample, lysis, and elution tubes. The workflow has been designed for a completely hands-free operation enabling reproducible extraction efficiencies and reduced time-to-results.

PrepSEQ™ Rapid Spin Sample Preparation Kit with Proteinase K Applied Biosystems™

The PrepSEQ™ Rapid Spin Sample Preparation Kit with Proteinase K is recommended for difficult to lyse bacteria like Listeria monocytogenes. The kit was developed for the rapid preparation of DNA from food and environmental samples for use in PCR amplification reactions. Using a simple spin protocol, PrepSEQ™ Rapid Spin efficiently inactivates PCR inhibitors. For laboratories involved in food and environmental testing, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. This has created the demand for a sample preparation method that is fast, cost-effective and capable of extracting high-quality DNA from a broad range of sample types. While such methods have been developed for dedicated, high-throughput laboratories, there is a growing need for methods suitable to low-to-medium throughput applications. The PrepSEQ™ Rapid Spin Kit with Proteinase K meets this need.
• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput work loads (up to 100 samples a day)
• Inactivates PCR inhibitors so you will most likely not have to repeat the template preparation step
• Uses a simple spin protocol

Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from a broad range of sample types including chocolate, soft cheeses, and soil. Even samples with difficult-to-lyse bacteria, such as Listeria monocytogenes, produce excellent results with the addition of a Proteinase K digestion step.

Designed for Laboratories that Prepare up to 100 Samples a Day
Kits exist for dedicated, high-throughput laboratories, but now you have a kit that is suitable for low-to-medium throughput applications. This kit is designed for laboratories that routinely prepare up to 100 samples a day.

Removes PCR Inhibitors
The PrepSEQ™ Rapid Spin Sample Preparation Kit offers a simple, cost-effective way to prepare bacterial DNA from broth cultures. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube where it can be stored or used for PCR.


.
For Research Use Only. Not for use in diagnostics procedures.

resDNASEQ™ Quantitative NS0 DNA Kit with PrepSEQ™ Residual DNA Sample Preparation Kit Applied Biosystems™

The resDNASEQ® Quantitative NS0 DNA kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from NS0 (murine myeloma) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of NS0 cell DNA, typically in less than 4 hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high molecular weight or sheared DNA.

This kit is bundled together with sample preparation reagents in the form of a PrepSEQ® Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts. For the quantitation kit alone without the sample preparation kit, please see resDNASEQ® Quantitative NS0 DNA Kit.

• Highly sensitive quantitation using proven TaqMan® real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Integrated system includes sample preparation kit, master mix, TaqMan® primer/probe mix, and NS0 genomic DNA standard

The resDNASEQ® Quantitative NS0 DNA Kit is designed to provide highly sensitive detection of NS0 DNA, allowing you to use small sample volumes to generate accurate results. The broad linear range of TaqMan® real-time qPCR technology allows you to test samples with variable levels of NS0 DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts.

Easy to use, results in under 4 hours
The easy workflow of the resDNASEQ® Quantitative NS0 DNA Kit consists of sample preparation, assay setup, and instrument run and data analysis—all of which you can perform in under 4 hours. Start by extracting DNA from your in-process purification and drug substance samples. Then perform qPCR analysis to compare the amount of NS0 DNA in your test samples to a standard curve generated with known amounts of purified NS0 genomic DNA standard. The resDNASEQ® system includes almost everything you need to carry out these steps, from sample preparation kit to master mix to TaqMan® primer/probe mix to NS0 genomic DNA standard, as well as step-by-step instructions.

Specific to NS0 DNA, no cross-reactivity with unrelated DNA
The target of the assay is highly specific—it only detects a NS0-specific region of a multicopy genetic element. We selected this region using extensive bioinformatic analysis of multiple related and unrelated species.

Consistent performance even with fragmented DNA
For accurate quantitation of residual NS0 DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ® system you get consistent assay performance across a range of DNA fragment sizes.

TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 10 mL PCR) Applied Biosystems™

The Applied Biosystems® TaqMan® Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hour. The TaqMan® Sample-to-SNP™ Kit combines DNA extraction and TaqMan® chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan®™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 400 sample preparation reactions at 50 µL/ reaction and 4,000 PCR reactions at 5 µL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan® Sample-to-SNP™ Kit.
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