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PrepFiler™ 96-Well Spin Plates and Filter Plates, ethylene oxide-treated (Applied Biosystems™)

PrepFiler 96-Well Spin Plates and Filter Plates are intended for use with the updated PrepFiler Automated Forensic DNA Extraction Kit, which does not include plastic consumables, providing users the flexibility to order only the plastics consumables they need. PrepFiler Forensic DNA Extraction kits use magnetic particles with an optimized multi-component surface chemistry to deliver superior DNA yield from most forensic sample types, including body fluids, and stains and swabs of body fluids. The PrepFiler Spin Plates and Filter Plates are used for sample lysis and substrate removal for sample processing in 96-well plate format. The packaged plates are treated with ethylene oxide.

PrepFiler™ Spin Tubes and Filter Columns, ethylene oxide-treated (Applied Biosystems™)

PrepFiler Spin Tubes and Filter Columns are available here as a separate purchase, providing ordering flexibility and allowing researchers to select only the plastic consumables needed for use with all PrepFiler forensic DNA extraction kits. These kits use magnetic particles with an optimized multi-component surface chemistry to deliver superior DNA yield from most forensic sample types, including body fluids, stains, and swabs of body fluids.

PrepFiler Filter Columns are used for substrate removal, and PrepFiler Spin Tubes are used for sample processing in tube format. The packaged Spin Tubes and Filter Columns are treated with ethylene oxide.

PrepFiler forensic DNA extraction kits contain other components required for DNA recovery from both routine and challenging samples, including removal of PCR inhibitors.

Pierce™ Spin Columns - Screw Cap (Thermo Scientific™)

Thermo Scientific Pierce Screw Cap Spin Columns are convenient tools for manipulating small volumes of affinity supports (20 to 400 µL) for protein purification.

Features of Screw Cap Spin Columns:

Adaptable: included Luer-Lok adaptors allow these columns to be used for syringe-based purifications
Column volume: 900µL
Resin volume: 20 to 400µL
Filter type: polyethylene filter, ~10µm pore size
Cap: O-ring screw-top caps
Frit options: included small & large frit options for different sample sizes

Simply add the affinity resin and sample to one of the spin columns, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Spin columns allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

Related Products
Pierce™ Spin Cups - Paper Filter
Pierce™ Spin Cups - Cellulose Acetate Filter
Pierce™ Spin Columns - Snap Cap
Pierce™ Micro-Spin Columns

Pierce™ Centrifuge Columns, 0.8 mL (Thermo Scientific™)

Thermo Scientific Pierce Centrifuge Columns are convenient tools for handling 40 µL to 10 mL of an affinity support for purification.

Column details:

• Made from low protein binding polypropylene for compatibility with protein purification
• Total volume: 800 µL
• Resin volume: 40-400 µL
• Filter type, pore size: Polyethylene filter, ~30 µm pore size
• Receiver tube: Fits standard microcentrifuge tubes
• Caps: O-ring screw-top cap
• Twist-off bottom

Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results.

Applications:

• Affinity purification, affinity chromatography
• Immunodepletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange

Related Products
Pierce™ Centrifuge Columns, 2 mL
Pierce™ Centrifuge Columns, 5 mL
Pierce™ Centrifuge Columns, 10 mL
Column Extenders for Pierce™ Centrifuge Columns

Antibody Conjugate Purification Kit, 20-50 µg conjugate (Invitrogen™)

Molecular Probes® Antibody Conjugate Purification kits efficiently purify antibody conjugates from unconjugated dye or biotin. This particular kit is optimized for the purification of 20–50 µg of antibody conjugate. Purification is accomplished using an easy-to-assemble size-exclusion spin column containing BioGel® P-6 fine resin. Each kit contains sufficient material for 3 separate purifications, and can also be used to purify proteins with a molecular weight greater than 12 kDa away from unconjugated labels.

Additional Features of this Antibody Conjugate Purification Kit:

• Purified conjugate ready-to-use in ~10 minutes with ~5 minutes of hands-on time

• Designed for low-molecular weight labels (<3000 Da, e.g. Alexa Fluor® dyes); cannot be used for removing unconjugated high-molecular weight labels (>8 kDa) such as enzymes (HRP) or proteins (R-phycoerythrin)

More about Protein and Antibody Labeling

We offer a wide selection of Molecular Probes® antibody and protein labeling kits to fit your starting material and your experimental set-up. To learn more about our labeling kits, see Kits for Labeling Proteins and Nucleic Acids in The Molecular Probes® Handbook or visit our antibody labeling page.

Antibody Conjugate Purification Kit, 50-100 µg conjugate (Invitrogen™)

Molecular Probes® Antibody Conjugate Purification kits efficiently purify antibody conjugates from unconjugated dye or biotin. This particular kit is optimized for the purification of 50–100 µg of antibody conjugate. Purification is accomplished using an easy-to-assemble size-exclusion spin column. Each kit contains sufficient material for 5 separate purifications, and can also be used to purify high-molecular weight proteins (> 30 kDa) away from unconjugated labels.

Additional Features of this Antibody Conjugate Purification Kit:

• Purified conjugate ready-to-use in ~45 minutes with ~15 minutes of hands-on time

• Designed for low-molecular weight labels (<3000 Da, e.g. Alexa Fluor® dyes); cannot be used for removing unconjugated high-molecular weight labels (>10 kDa) such as enzymes (HRP) or proteins (R-phycoerythrin)

More about Protein and Antibody Labeling

We offer a wide selection of Molecular Probes® antibody and protein labeling kits to fit your starting material and your experimental set-up. To learn more about our labeling kits, see Kits for Labeling Proteins and Nucleic Acids in The Molecular Probes® Handbook or visit our antibody labeling page.

Microcentrifuge Spin Columns (Invitrogen™)

The microcentrifuge spin columns included with the FastTrack® 2.0 and Micro-FastTrack™ 2.0 Kits are available separately. The columns are made from polypropylene and are designed to enhance the speed and efficiency of polyA+ RNA isolation. The columns are sterilized and RNase-free.

Pierce™ Spin Columns - Snap Cap (Thermo Scientific™)

Thermo Scientific Pierce Snap Cap Spin Columns are convenient tools for manipulating small volumes of affinity supports (20 to 500 µL) for protein purification.

Features of Snap Cap Spin Columns:

Formulation: polypropylene
Column volume: 1 mL
Resin volume: 20 to 500µL
Filter type: Polyethylene filter, ~30 µm pore size
Cap: Attached snap-cap (no cap on collection tube); press-on bottom caps

Simply add the affinity resin and sample to one of the spin columns, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Spin columns allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

Related Products
Pierce™ Spin Columns - Screw Cap
Pierce™ Spin Cups - Paper Filter
Pierce™ Spin Cups - Cellulose Acetate Filter
Pierce™ Micro-Spin Columns

POROS™ GoPure™ HS Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS HS 50 µm resin in this GoPure column has a sulphopropyl functional group for strong cation exchange chromatography.

Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

POROS™ GoPure™ MabCapture A Select Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

POROS GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) and hydrophobic interaction chromatography (HIC) resins or for small-scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS MabCapture A Select 50 µm resin in this GoPure column has a CaptureSelect protein A ligand immobilized to POROS backbone suitable for capture affinity chromatography.

Other POROS hydrophobic interaction and ion exchange resins are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

Pierce™ Centrifuge Columns, 5 mL (Thermo Scientific™)

Thermo Scientific Pierce Centrifuge Columns are convenient tools for handling 40 µL to 10 mL of an affinity support for purification.

Column details:

• Made from low protein binding polypropylene for compatibility with protein purification
• Total volume: 8 mL (5 mL resin bed, 3 mL reservoir)
• Resin volume: 5 mL
• Filter type, pore size: Polyethylene filter, ~30 µm pore size
• Receiver tube: Fits standard 15 mL conical centrifuge tubes
• Caps: Screw-top cap
• Twist-off bottom and press-on cap to reseal

Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results.

Applications:

• Affinity purification, affinity chromatography
• Immunodepletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange

Related Products
Pierce™ Centrifuge Columns, 0.8 mL
Pierce™ Centrifuge Columns, 2 mL
Pierce™ Centrifuge Columns, 10 mL
Column Extenders for Pierce™ Centrifuge Columns

POROS™ GoPure™ XQ Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

POROS GoPure 0.5 cm diameter x 5 cm long prepacked columns are intended for screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification. These prepacked columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS XQ resin in this GoPure column features a strong anion exchange functional group and is a high capacity and high resolution resin with excellent salt tolerance.

Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS high performance chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

Pierce™ Spin Cups - Paper Filter (Thermo Scientific™)

Thermo Scientific Pierce Paper Filter Spin Cups are convenient tools for manipulating small volumes of affinity supports (20 to 300 µL) for protein purification.

Features of Paper Filter Spin Cups:

Resistant: paper filters are resistant to clogging from cellular debris
Column volume: 600µL
Resin volume: 20 to 300µL
Filter type: paper filter, ~10µm pore size
Cap: collection tube cap fits onto inserted spin cup

Simply add the affinity resin and sample to one of the spin cups, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Spin cups allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

Related Products
Pierce™ Spin Columns - Screw Cap
Pierce™ Spin Cups - Cellulose Acetate Filter
Pierce™ Spin Columns - Snap Cap
Pierce™ Micro-Spin Columns

Centri-Sep™ Spin Columns (Invitrogen™)

Centri-Sep™ Spin Columns are used for the fast and efficient purification of nucleic acids. The Centri-Sep™ gel provides excellent recovery of DNA fragments larger than 16 base pairs, while removing greater than 98% of salts, NTPs, and other low molecular weight impurities. Features of Centri-Sep™ columns:

• Processes samples faster than ultrafiltration columns
• Achieves rapid and efficient separation
• Permits use of custom buffers
• Convenient 20–100 µL sample size
• Dry gel format
• Room temperature storage
• Microcentrifuge format

Designed for performance
The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri-Sep™ unit consists of a specially fritted microfuge tube, dry gel, a wash tube, and a sample collection tube, all specifically designed for this purpose.

Excellent recovery
The Centri-Sep™ gel provides excellent recovery of DNA fragments with sizes greater than 16 base pairs or a 25-mer, while removing >98% of salts, NTPs, and other unwanted low molecular weight impurities.

Versatile
The column gel is hydrated with either reagent-grade water or a buffer of your choice, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the intersititial fluid. Your sample is then applied to the column and spun again, leaving you not only with a sample purified through removal of low molecular weight components, but also exchanged into the buffer of your choice.

POROS™ GoPure™ XS Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS XS resin in this GoPure column has a sulphopropyl functional group for strong cation exchange chromatography.

Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.