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PrepFiler™ Spin Tubes and Filter Columns, ethylene oxide-treated (Applied Biosystems™)

PrepFiler Spin Tubes and Filter Columns are available here as a separate purchase, providing ordering flexibility and allowing researchers to select only the plastic consumables needed for use with all PrepFiler forensic DNA extraction kits. These kits use magnetic particles with an optimized multi-component surface chemistry to deliver superior DNA yield from most forensic sample types, including body fluids, stains, and swabs of body fluids.

PrepFiler Filter Columns are used for substrate removal, and PrepFiler Spin Tubes are used for sample processing in tube format. The packaged Spin Tubes and Filter Columns are treated with ethylene oxide.

PrepFiler forensic DNA extraction kits contain other components required for DNA recovery from both routine and challenging samples, including removal of PCR inhibitors.

PrepFiler™ 96-Well Spin Plates and Filter Plates, ethylene oxide-treated (Applied Biosystems™)

PrepFiler 96-Well Spin Plates and Filter Plates are intended for use with the updated PrepFiler Automated Forensic DNA Extraction Kit, which does not include plastic consumables, providing users the flexibility to order only the plastics consumables they need. PrepFiler Forensic DNA Extraction kits use magnetic particles with an optimized multi-component surface chemistry to deliver superior DNA yield from most forensic sample types, including body fluids, and stains and swabs of body fluids. The PrepFiler Spin Plates and Filter Plates are used for sample lysis and substrate removal for sample processing in 96-well plate format. The packaged plates are treated with ethylene oxide.

Pierce™ Micro-Spin Columns (Thermo Scientific™)

Thermo Scientific Pierce Micro-Spin Columns are convenient tools for manipulating small volumes of affinity supports (5 to 100 µL) for protein purification.

Features of Micro-Spin Columns:

Small volume: ideal for very small resin and sample volumes
Column volume: 400 µL
Resin volume: 5 to 100 µL
Filter type: Polyethylene filter, ~30 µm pore size
Cap: O-ring screw top caps; press-on bottom caps

Simply add the affinity resin and sample to one of the micro-spin columns, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Micro-spin columns allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

Related Products
Pierce™ Spin Columns - Screw Cap
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POROS™ GoPure™ AAV9 Pre-packed Column, 0.5 x 5 cm, 1 mL (Thermo Scientific™)

GoPure 0.5-cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS CaptureSelect AAV9 50 µm resin in this GoPure column has a novel AAV9 CaptureSelect ligand, making it suited primarily for affinity capture of adeno-associated virus (AAV9) serotypes.

• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the GoPure family of pre-packed columns simplifies process scale-up and process modeling

GoPure 0.5-cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS resin-screening platform. The 1-mL bed volume reduces sample requirements, and the recommended > 3-minute residence time (0.3 mL/min) on this 5-cm-length column equates to 400 cm/hr on a 20-cm bed height. Detailed specifications may be found in the GoPure 0.5-cmD Operating Instructions below.

Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, GoPure Pre-Packed Columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 ml to 6.3 liters.

Pierce™ Centrifuge Columns, 10 mL (Thermo Scientific™)

Thermo Scientific Pierce Centrifuge Columns are convenient tools for handling 40 µL to 10 mL of an affinity support for purification.

Column details:

• Made from low protein binding polypropylene for compatibility with protein purification
• Total volume: 22 mL (10 mL resin bed, 12 mL reservoir)
• Resin volume: 10 mL
• Filter type, pore size: Polyethylene filter, ~30 µm pore size
• Receiver tube: Fits standard 50 mL conical centrifuge tubes
• Caps: Screw-top cap
• Twist-off bottom and press-on cap to reseal

Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results.

Applications:

• Affinity purification, affinity chromatography
• Immunodepletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange

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POROS™ GoPure™ AAVX Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

POROS GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) and hydrophobic interaction chromatography (HIC) resins or for small-scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS CaptureSelect AAVX 50 µm resin in this GoPure column has a novel AAVX CaptureSelect ligand, making it suited primarily for affinity capture of a broad range of adeno-associated virus (AAV) serotypes.

Other POROS hydrophobic interaction and ion exchange resins are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

POROS™ GoPure™ HS Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS HS 50 µm resin in this GoPure column has a sulphopropyl functional group for strong cation exchange chromatography.

Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

POROS™ GoPure™ Ethyl Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

POROS GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) and hydrophobic interaction chromatography (HIC) resins or for small-scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS Ethyl HIC resin in this GoPure column has a novel hydrophobic ethyl ligand, making it suited primarily for bind/elute purifications.

Other POROS hydrophobic interaction and ion exchange resins are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

Pierce™ Spin Cups - Paper Filter (Thermo Scientific™)

Thermo Scientific Pierce Paper Filter Spin Cups are convenient tools for manipulating small volumes of affinity supports (20 to 300 µL) for protein purification.

Features of Paper Filter Spin Cups:

Resistant: paper filters are resistant to clogging from cellular debris
Column volume: 600µL
Resin volume: 20 to 300µL
Filter type: paper filter, ~10µm pore size
Cap: collection tube cap fits onto inserted spin cup

Simply add the affinity resin and sample to one of the spin cups, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Spin cups allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

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Centri-Sep™ Spin Columns (Invitrogen™)

Centri-Sep™ Spin Columns are used for the fast and efficient purification of nucleic acids. The Centri-Sep™ gel provides excellent recovery of DNA fragments larger than 16 base pairs, while removing greater than 98% of salts, NTPs, and other low molecular weight impurities. Features of Centri-Sep™ columns:

• Processes samples faster than ultrafiltration columns
• Achieves rapid and efficient separation
• Permits use of custom buffers
• Convenient 20–100 µL sample size
• Dry gel format
• Room temperature storage
• Microcentrifuge format

Designed for performance
The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri-Sep™ unit consists of a specially fritted microfuge tube, dry gel, a wash tube, and a sample collection tube, all specifically designed for this purpose.

Excellent recovery
The Centri-Sep™ gel provides excellent recovery of DNA fragments with sizes greater than 16 base pairs or a 25-mer, while removing >98% of salts, NTPs, and other unwanted low molecular weight impurities.

Versatile
The column gel is hydrated with either reagent-grade water or a buffer of your choice, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the intersititial fluid. Your sample is then applied to the column and spun again, leaving you not only with a sample purified through removal of low molecular weight components, but also exchanged into the buffer of your choice.

PureLink™ Nucleic Acid Purification Rack (Invitrogen™)

Convenient large capacity fits multiple-sized column

• The compact nesting feature is convenient for storage

The PureLink® Nucleic Acid Purification Rack is designed for use with columns in the PureLink® HiPure Plasmid Purification Systems. The rack includes both a Collection Tube Rack for holding various sized recovery tubes (Figure 1) and a large-capacity Waste Reservoir. One rack holds as many as 12 miniprep columns, 8 midiprep columns, and 4 maxiprep columns. When you're finished, the Waste Reservoir nests into to the Collection Tube Rack for compact storage.

NucAway™ Spin Columns (Invitrogen™)

Ambion® NucAway™ Spin Columns are for the fast removal of unincorporated nucleotides and salts from probes after probe synthesis reactions. 30 columns are provided.
• Fast, efficient removal of unincorporated nucleotides and salts after probe synthesis
• RNase- and DNase-free
• Replaces more time- consuming purification methods To use, simply rehydrate the column, centrifuge to remove the interstitial fluid, add the probe to the top of the column, and centrifuge again to elute the probe.

PureLink™ 96 Receiver Plates (Invitrogen™)

PureLink® 96: Sample size 1.3 ml culture; Typical yields up to 3-5 µg
PureLink® 384: Sample size 200 µl culture; Typical yields up to 2-3 µg

––Purify 96 or 384 plasmid DNA samples simultaneously
–centrifugation method eliminates cross-contamination


The PureLink® 96 Plasmid Purification System is designed for high-throughput isolation of high-copy number plasmids. Following the PureLink® 96 Plasmid Purification Systems protocol, up to 96 plasmid DNA samples can be simultaneously purified. The novel technology eliminates time-consuming cell harvesting and alkaline lysis steps, saving time and effort. The unique centrifugationbased format of the PureLink® 96 Plasmid Purification System eliminates opportunities for cross-contamination between wells, a critical factor in PCR and microarrays. The isolated plasmid DNA is ideal for use in automated sequencing reactions, offering superior read lengths and data quality. Samples may be processed 96 at a time or in smaller increments. The PureLink® 96 Plasmid Purification System is compatible with a variety of centrifuges, robots, pipetting devices, automated sequencers, and sequencing chemistries.

The PureLink® 384 Plasmid Purification Plate is designed for ultra highthroughput DNA isolation. By utilizing the same technology as the PureLink® 96 Plasmid Purification System, the PureLink® 384 Plasmid Purification Plate reduces time and effort while reliably isolating high-quality plasmid DNA for DNA sequencing, PCR, and restriction digestion.

POROS™ GoPure™ MabCapture A Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

POROS GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) and hydrophobic interaction chromatography (HIC) resins or for small-scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS MabCapture A 50µm resin in this GoPure column has a protein A ligand immobilized to POROS backbone suitable for capture affinity chromatography.

Other POROS hydrophobic interaction and ion exchange resins are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

Pierce™ Centrifuge Columns, 2 mL (Thermo Scientific™)

Thermo Scientific Pierce Centrifuge Columns are convenient tools for handling 40 µL to 10 mL of an affinity support for purification.

Column details:

• Made from low protein binding polypropylene for compatibility with protein purification
• Total volume: 5 mL (2 mL resin bed, 3 mL reservoir)
• Resin volume: 2 mL
• Filter type, pore size: Polyethylene filter, ~30 µm pore size
• Receiver tube: Fits standard 15 mL conical centrifuge tubes
• Caps: Screw-top cap
• Twist-off bottom and press-on cap to reseal

Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results.

Applications:

• Affinity purification, affinity chromatography
• Immunodepletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange

Related Products
Pierce™ Centrifuge Columns, 0.8 mL
Pierce™ Centrifuge Columns, 5 mL
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Column Extenders for Pierce™ Centrifuge Columns