Regulatory requirements vary by product. Please see product-specific literature to determine product use.

Product FAQ

Which fluorochromes are recommended for antibody staining in the PrimeFlow RNA Assay?

Answer

Most organic and protein-based fluorochromes are compatible with this assay kit, including PE, PE tandems, APC, APC tandems, and small organic dyes such as FITC, eFluor 450, eFluor 506, eFluor 660, and Alexa Fluor 700. However, PerCP, PerCP-Cyanine5.5, and PerCP-eFluor 710 may not be used, and we recommend using PE-Cyanine5 or PE-Cyanine5.5 instead. Qdot nanocrystal and eVolve-antibody conjugates are not compatible with this assay. If Super Bright or Brilliant Violet conjugated antibodies will be utilized, please contact Tech Support at techsupport@thermofisher.com for assistance with optimizing your multicolor panel.

Answer Id: E14578

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