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Products

WesternBreeze™ Chromogenic Kit, anti-mouse Invitrogen™

WesternBreeze® Chromogenic Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use BCIP/NBT substrate for alkaline phosphatase. Non-fading purple precipitates develop at the protein bands on the membrane. WesternBreeze® chromogenic offers:

• Clear background
• High sensitivity-low picogram levels detectable
• High specificity
• One simple protocol-no optimization required

SuperSignal™ West Pico Rabbit IgG Detection Kit Thermo Scientific™

Thermo Scientific SuperSignal West Pico Complete Rabbit IgG Detection Kit is a a kit containing goat anti-rabbit secondary antibody HRP conjugate for the detection of rabbit primary antibody. The kit is ideal for busy researchers or new beginners to Western blot analysis.

Features of SuperSignal West Pico Complete Rabbit IgG Detection Kit:

Optimized—secondary antibodies are validated for use with the SuperSignal West Pico Chemiluminescent Substrate, eliminating the need to test multiple dilutions
Large packages—kits provide sufficient reagents to complete up to 50 experiments with standard mini gel blots (4000 square centimeters of membrane)
Standard or complete—choose a kit with substrate and antibody only, or a complete kit that also includes TBS wash buffer and high-performance SuperBlock Blocking Buffer

All the components of these Western blotting kits are optimized for compatibility the SuperSignal West Pico Chemiluminescent Substrate. The kits are offered in a standard format (substrate and optimized HRP-conjugates) and a complete format that also includes the Thermo Scientific SuperBlock Buffer and BupH Tris Buffered Saline.

Related Products
SuperSignal™ West Pico Complete Mouse IgG Detection Kit
SuperSignal™ West Pico Mouse IgG Detection Kit
SuperSignal™ West Pico Complete Rabbit IgG Detection Kit

WesternBreeze™ Chemiluminescent Kit, anti-mouse Invitrogen™

WesternBreeze® Chemiluminescent Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use CDP-Star® chemiluminescent substrate for alkaline phosphatase. Protein bands can be captured either by X-ray film or CDP-Star® compatible imaging system. WesternBreeze® Chemiluminescent Kit offers:

• High specificity, clean background
• Ultra-sensitivity-femtogram levels detectable
• Long-lasting signals-up to 5 days
• Results in less than 3 hours
• Permanent photographic image

SuperSignal™ West Pico Complete Rabbit IgG Detection Kit Thermo Scientific™

Thermo Scientific SuperSignal West Pico Complete Rabbit IgG Detection Kit is a a kit containing goat anti-rabbit secondary antibody HRP conjugate for the detection of rabbit primary antibody. The kit is ideal for busy researchers or new beginners to Western blot analysis.

Features of SuperSignal West Pico Complete Rabbit IgG Detection Kit:

Optimized—secondary antibodies are validated for use with the SuperSignal West Pico Chemiluminescent Substrate, eliminating the need to test multiple dilutions
Large packages—kits provide sufficient reagents to complete up to 50 experiments with standard mini gel blots (4000 square centimeters of membrane)
Standard or complete—choose a kit with substrate and antibody only, or a complete kit that also includes TBS wash buffer and high-performance SuperBlock Blocking Buffer

All the components of these Western blotting kits are optimized for compatibility the SuperSignal West Pico Chemiluminescent Substrate. The kits are offered in a standard format (substrate and optimized HRP-conjugates) and a complete format that also includes the Thermo Scientific SuperBlock Buffer and BupH Tris Buffered Saline.

Related Products
SuperSignal™ West Pico Complete Mouse IgG Detection Kit
SuperSignal™ West Pico Mouse IgG Detection Kit
SuperSignal™ West Pico Rabbit IgG Detection Kit

WesternBreeze™ Chromogenic Kit, anti-goat Invitrogen™

WesternBreeze® Chromogenic Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use BCIP/NBT substrate for alkaline phosphatase. Non-fading purple precipitates develop at the protein bands on the membrane. WesternBreeze® chromogenic offers:

• Clear background
• High sensitivity-low picogram levels detectable
• High specificity
• One simple protocol-no optimization required

Novex™ AP Mouse Chemiluminescent Detection Kit Invitrogen™

The Novex® AP Mouse Chemiluminescent Detection Kit provides superior chemiluminescent detection of proteins transferred to either nitrocellulose or PVDF membranes and treated with a protein-specific primary antibody. Detection is performed by treatment of the membrane with an alkaline phosphatase (AP)-conjugated anti-mouse secondary antibody followed by a ready-to-use chemiluminescent CDP-Star® substrate of alkaline phosphatase. Protein-specific signal is then captured by a chemiluminescent-compatible imaging system or X-ray film.

The Novex® AP Mouse Chemiluminescent Detection Kit is compatible with most western protocols but was designed for use with the iBind™ Western System.

Each kit includes a secondary AP-conjugated anti-mouse antibody, CDP-Star® chemiluminescent substrate, and an enhancer, sufficient for 10 mini-membranes processed using the iBind™ Western System.

Features include:

• Easy-to-use protocol
• High specificity, clean background
• Ultra-sensitivity
• Long-lasting signals—up to 5 days
• Results in less 5 minutes

iBind™ Flex Fluorescent Detection (FD) Solution Kit Invitrogen™

iBind Flex Fluorescent Detection (FD) Solution is compatible with downstream fluorescent detection (e.g., infrared dual wavelength) and serves as a combined blocking, washing, and antibody diluent solution to process up to one midi blot, two mini blots, or six vertically cut strip blots when used with the iBind Flex Western Device and iBind Flex Cards. Each kit contains reagents sufficient for 10 midi blots or 20 mini blots.

Learn more about the iBind western systems ›

iBind Flex Western System
The iBind Flex Western System offers automated western blot processing and can accommodate various blot formats including midi, mini, and vertically cut strips. Based on Sequential Lateral Flow (SLF) technology, all blocking, antibody incubation and washing steps during immunodetection are hands-free. Compared to manual methods, the iBind Flex Western System requires less primary antibody, enables reproducible results and is compatible with downstream detection protocols.

Prionics™-Check WESTERN Kit (12000)

The Prionics®-Check WESTERN Kit is the gold standard of rapid BSE testing and is used as a confirmatory test by most National Reference Laboratories across Europe. It owes its success to its precision and reliability. The test uses the relative protease resistance of the disease-specific prion protein PrPSc for TSE detection. Protease digestion leads to a small decrease in PrPSc protein size, whereas the normal protein PrPC is completely degraded.

The Prionics®-Check WESTERN Kit delivers a completely reliable test-result based on three independent and easily interpretable criteria: signal (PrPSc), characteristic three-band pattern BSE positive, position relative to control (PrPC).

Test procedure
1. The homogenized brain sample is incubated with an optimized reagent mixture consisting of digestion enzymes and a buffer solution. This solution degrades the normal prion protein. Only the BSE-specific prion protein remains in the test sample.
2. The proteins in the sample are then separated according to size by gel electrophoresis and transferred to a special blot membrane for detection. The BSE-specific prion protein on the membrane is detected with specific antibodies and is visualized on a digital file or on a film.

SuperSignal™ West HisProbe Kit Thermo Scientific™

The Thermo Scientific SuperSignal West HisProbe Kit contains HisProbe-HRP Conjugate, as well as reagents required for detection of polyhistidine-tagged protein by western blotting. HisProbe-HRP Conjugate is a nickel (Ni2+)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins.

Features of the SuperSignal West HisProbe Kit:

Fast—His-tag detection is achieved with a one-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol
Economical—no need to purchase expensive primary/secondary antibodies; considerably less costly than anti-6xHis-tag antibodies
High specificity—HisProbe-HRP can recognize his-tags independent of adjacent tags.
Less steric hindrance—HisProbe-HRP is smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies

Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags. The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC). The Thermo Scientific SuperSignal West HisProbe Kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate.

Related Products
HisProbe™-HRP Conjugate

WesternBreeze™ Chromogenic Kit, anti-rabbit Invitrogen™

WesternBreeze® Chromogenic Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use BCIP/NBT substrate for alkaline phosphatase. Non-fading purple precipitates develop at the protein bands on the membrane. WesternBreeze® chromogenic offers:

• Clear background
• High sensitivity-low picogram levels detectable
• High specificity
• One simple protocol-no optimization required

iBind™ Solution Kit Invitrogen™

The iBind Solutions Kit is intended for use with the iBind Western Device, which is an automated western-processing device that performs every step in the western workflow from blocking to washes to antibody incubations. The iBind solutions act as the blocking, washing, and dilution solutions in the iBind immunodetection process and have been optimized for ideal viscosity. Each solution is released from the iBind device wells to the iBind Card through sequential lateral flow (SLF); the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction.

Learn more about the iBind western systems ›

The iBind Solutions Kit, together with the iBind Western Device and iBind Cards, bring automation and simplicity to the western protocol. Each iBind Solutions Kit contains sufficient solution for 10 mini-membrane reactions. An iBind Western Device is required for use of this kit. For fluorescent applications use the iBind Fluorescent Detection (FD) Solution Kit.

WesternBreeze™ Chemiluminescent Kit, anti-goat Invitrogen™

WesternBreeze® Chemiluminescent Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use CDP-Star® chemiluminescent substrate for alkaline phosphatase. Protein bands can be captured either by X-ray film or CDP-Star® compatible imaging system. WesternBreeze® Chemiluminescent Kit offers:

• High specificity, clean background
• Ultra-sensitivity-femtogram levels detectable
• Long-lasting signals-up to 5 days
• Results in less than 3 hours
• Permanent photographic image

Pierce™ S-Nitrosylation Western Blot Kit Thermo Scientific™

Thermo Scientific Pierce S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications in a complete, easy-to-use kit.

Features of Pierce S-Nitrosylation Western Blot Kit:

Specific—optimized labeling reactions switch S-nitrosocysteine post translational modifications with iodoTMT Reagent for easy detection by Western blot using anti-TMT antibody
Better signal-to-noise—uses a non-biological iodoTMT Reagent for labeling instead of HPDP-biotin, resulting in less background during Western blot detection
Workflow-compatible—labeling method is compatible with alternative methods of analysis, including enrichment via anti-TMT resin and multiplex quantitation by mass spectrometry
Convenient—all needed reagents included one kit

Includes:
• Cysteine-blocking reagent, reducing agent, reaction buffer, iodoTMT™ labeling reagent, and anti-TMT antibody

The Pierce S-Nitrosylation Western Blot Kit contains a cell lysis/reaction buffer, a sulfhydryl-reactive blocking agent, a reducing agent, a labeling agent and a detection monoclonal antibody. Each kit has enough reagents to label 40 samples of 100 µg each. Similar to the traditional S-nitrosylation switch assay, unmodified cysteines are first blocked using a sulfhydryl-reactive compound (MMTS). S-nitrosylated cysteines are then selectively reduced with ascorbate in HENS Buffer for specific labeling with iodoTMTzero™ Reagents, which irreversibly bind to the cysteine thiol that was S-nitrosylated. Detection of the TMT™ reagent-modified proteins is facilitated using an anti-TMT antibody.

S-nitrosylation is a post-translational modification that regulates numerous processes, including cellular proliferation, apoptosis, smooth muscle relaxation, neurotransmitter release, and differentiation (Ref.1). During S-nitrosylation, nitric oxide radicals react with cysteine thiols to produce an S-NO adduct that alters protein activity.

In 1998, Jaffrey et al. described the biotin switch assay, which used HPDP-biotin to measure in vivo and in vitro S-nitrosylation (Ref.2). In this assay, free sulfhydryls are chemically blocked, whereas nitrosylated cysteines are selectively reduced and biotinylated for Streptavidin-HRP Western blot detection. However, most cells produce biotinylated polypeptides which yield false-positive signals during Western blot analysis. In addition, the biotin labeling chemistry is reversible, preventing S-nitrosylation site identification by mass spectrometry.

The Pierce S-Nitrosylation Western Blot Kit provides all of the necessary reagents for a modified form of the traditional S-nitrosylation switch assay. Labeling is accomplished using the non-biological iodoTMT™ Reagent instead of HPDP-biotin, and this results in less background during Western blot detection. In addition to detection of S-nitrosylated proteins by Western blot, an immobilized anti-TMT resin can be used to selectively enrich S-nitrosylated proteins/peptides labeled with iodoTMT reagents. This workflow allows for S-nitrosylation site mapping and multiplexed quantititation using mass spectrometry.

More Product Data
Specific labeling, enrichment and quantitation of S-nitrosylated peptides using iodoTMT reagents

Related Products
HENS Buffer for Pierce™ S-Nitrosylation Western Blot Kit

Pierce™ Fast Western Blot Kit, ECL Substrate Thermo Scientific™

The Thermo Scientific Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

Features of this kit:

Fast—all the sensitivity of Pierce ECL substrate and saving 4 to 5 hours per blot
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Economical—cost as little as $8 per blot; no expensive consumables or extra equipment
Excellent stability—kit is stable for 1 year stored at 4°C
Easy—complete kit gives you all the components you need to block and probe and develop a blot with your mouse or rabbit primary antibody

The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The included Pierce ECL Substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed.

The Pierce Fast Western Blot Kit is reagent-based system that provides optimized reagents for blocking, antibody dilution and detection of Western blots with Pierce ECL substrate. The protocol is a quick, efficient and economic way to obtain Western blot results without the hassle of buying an instrument and the consumables required by other popular rapid Western blotting systems. No vacuum pump is needed so there are never any clogged lines or membranes that can occur when using instrument-based systems. Because there is no apparatus, there is no need to buy disposable blotting trays that limit the number of blots processed at one time.

Pierce™ Far-Western Blot Kit for Biotinylated Proteins Thermo Scientific™

The Thermo Scientific Pierce Far-Western Blot Kit for Biotinylated Proteins enables in-gel or on-membrane Western blot detection of target proteins via interaction with any biotin-labeled test protein used as the primary probe.

Features of the Far-Western Blot Kit for Biotinylated Proteins:

On-membrane or in-gel detection options—on-membrane detection offers greater sensitivity; in-gel detection is faster and prevents problems associated with incomplete or inefficient transfer
Nonradioactive alternative for far-Western analysis—reliable and sensitive biotin/streptavidin-HRP chemistry combined with chemiluminescent detection offers a practical and safe alternative to radiolabeling the bait protein
Useful interaction range—effective for interaction pairs that have moderate to strong binding associations between the prey and the biotinylated bait protein
Primary antibody-free detection—uses a biotinylated protein as the probe, eliminating the need for primary antibodies or even to have prior knowledge of target-protein identities
Compatible with both SDS-PAGE and native gels—provides option to probe for prey proteins in a more native environment because reduced or denaturing systems may not always allow for the intended interaction
Reduced nonspecific binding—biotin/streptavidin-HRP systems demonstrate less nonspecific binding compared to antibodies directed against the bait protein
Compatible with protein staining—gels can be used for total protein staining after the in-gel chemiluminescent detection step, eliminating the need to run two gels

This far-Western blotting kit provides the reagents and protocol necessary to probe for target proteins by virtue of their interaction with a specific binding partner, which the researcher supplies in purified, biotinylated form. The kit is successful for analysis of protein-protein interactions in which the target (prey) proteins can be bound after they have been separated by gel electrophoresis. The kit includes reagents and procedures for probing and chemiluminescent detection with blots or directly from mini gels.

Includes:
• Streptavidin-HRP conjugate, dilution and wash buffers, chemiluminescent substrate solutions, and cellophane sheets for mini gels

Applications:
• Protein interaction discovery—search and screen for proteins in cell lysates that have binding affinity to the purified biotinylated protein
• Protein interaction confirmation—characterize and identify the presence of native or off-target binding interactions involving a given biotinylated protein and particular samples

Far-Western blotting involves probing for targets first with a putative interaction-protein rather than a primary antibody. The method is called far-Western blotting because primary antibodies are used secondarily to detect the primary interaction probe rather than the target protein. The Pierce Far-Western Blot Kit for Biotinylated Proteins eliminates the use of antibodies altogether. The primary probe (i.e., the biotinylated bait protein supplied by the researcher) is secondarily detected with streptavidin-HRP conjugate, whose activity is visualized by reaction with a very sensitive chemiluminescent substrate.
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